FastQCFastQC Report
Thu 26 May 2016
SRR1926634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844967
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAG500.002499958715.9411041
GAACTAG400.002986273715.5227766
GTCCTAA1900.014.6825941
TGTAGGA4500.014.4964182
ATTAGAC450.004731165714.485143
CTAACCA450.0053260314.2289994
GTCCTAC2900.013.7423291
TACTATA1101.2754299E-613.5903922
CTGTAGG4850.013.4212041
GTCTAGG700.001510386813.2842531
TCTAACC755.918018E-513.0366263
TATAGGA853.7598272E-412.7909572
TAGGACC2100.012.7044624
GTCTTAA958.226877E-512.5850811
CCATGTA1953.274181E-1112.5449782
GTAGGAC4450.012.4507093
TCTACAC1151.5455043E-712.2808783
GTGCTGG1307.9680103E-712.2623871
GTCCTAT1102.4045374E-512.0765931
TCTAGGA1153.0014005E-511.8177322
Produced by FastQC (version 0.11.5)