##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926634_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 844967 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.082198476390204 27.0 7.0 31.0 2.0 31.0 2 16.379507128680764 16.0 2.0 30.0 2.0 31.0 3 19.742295261235054 23.0 15.0 30.0 2.0 31.0 4 21.810133413494256 28.0 2.0 35.0 2.0 35.0 5 22.433640603715883 28.0 2.0 35.0 2.0 35.0 6 23.35533577050938 30.0 2.0 35.0 2.0 37.0 7 23.23768502201861 30.0 7.0 35.0 2.0 37.0 8 23.30961919222881 30.0 7.0 35.0 2.0 37.0 9 25.980324675401526 31.0 17.0 37.0 2.0 39.0 10 26.68706825236962 32.0 22.0 37.0 2.0 39.0 11 23.849627263549937 30.0 7.0 37.0 2.0 39.0 12 24.940769284480933 31.0 10.0 37.0 2.0 39.0 13 21.66804739119989 27.0 2.0 35.0 2.0 39.0 14 24.930629243508918 30.0 13.0 38.0 2.0 40.0 15 25.984864497666774 31.0 17.0 38.0 2.0 40.0 16 26.361423582222738 31.0 16.0 38.0 2.0 40.0 17 25.648299874432965 31.0 10.0 38.0 2.0 40.0 18 25.70464408669214 31.0 10.0 38.0 2.0 40.0 19 26.100819321938015 31.0 11.0 38.0 2.0 40.0 20 26.357613965989206 31.0 10.0 39.0 2.0 40.0 21 24.225454958596018 30.0 2.0 37.0 2.0 40.0 22 25.141727428408448 31.0 2.0 38.0 2.0 40.0 23 25.112971275801304 31.0 2.0 38.0 2.0 40.0 24 24.229844479133504 31.0 2.0 38.0 2.0 40.0 25 24.01194247822696 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.249897956320524 1101 2 10.859949524601252 1101 3 7.36202312524728 1101 4 8.940530114578817 1101 5 8.598516534048837 1101 6 8.231677178249548 1101 7 8.057404230170722 1101 8 6.467107100102417 1101 9 6.700741897328587 1101 10 5.982350474201702 1101 11 8.66505358714954 1101 12 6.455949084361425 1101 13 10.757899219344925 1101 14 8.179972464430577 1101 15 7.495562243412792 1101 16 7.127924522436757 1101 17 2.66174390800688 1101 18 5.428961326681435 1101 19 6.275675424706332 1101 20 4.4498921673258245 1101 21 1.2589379462575572 1101 22 5.155558322137573 1101 23 6.395523150039239 1101 24 7.859671187910724 1101 25 8.124371172863203 1102 1 5.407173413200585 1102 2 10.315566825259065 1102 3 6.47714631019624 1102 4 7.852191973093319 1102 5 7.596418719306474 1102 6 6.604215131271168 1102 7 6.670863646620667 1102 8 6.884076633586435 1102 9 5.90134322562777 1102 10 5.241549403162043 1102 11 7.972351538396957 1102 12 5.333845856487599 1102 13 9.787096184008398 1102 14 7.239224981581966 1102 15 5.203684022576947 1102 16 5.62318169596745 1102 17 6.498591854230405 1102 18 6.619553793014564 1102 19 5.322938036714248 1102 20 5.658818674561587 1102 21 4.46820073749625 1102 22 5.504942012680253 1102 23 6.200972670777958 1102 24 7.650525168236978 1102 25 7.306123398085681 1103 1 4.727924137399949 1103 2 8.94463643059255 1103 3 5.927578559239986 1103 4 7.463237313263804 1103 5 6.617297549252793 1103 6 6.43733616599868 1103 7 6.619065057373284 1103 8 6.058436229733182 1103 9 5.21532854955003 1103 10 4.369477591285403 1103 11 5.122660767777994 1103 12 4.071242698665042 1103 13 8.21640211061348 1103 14 6.3580663352614515 1103 15 5.389096315551747 1103 16 5.159721805418165 1103 17 2.90547904806062 1103 18 2.0217772292366583 1103 19 3.891975045697091 1103 20 4.2380201320880175 1103 21 6.290423020267095 1103 22 2.0461290774964915 1103 23 3.9808891772556763 1103 24 5.3904904829588745 1103 25 1.6693274858442493 1104 1 5.666859341140764 1104 2 10.258023204139644 1104 3 6.722059521163835 1104 4 7.413328161627195 1104 5 7.602813915318258 1104 6 7.136042912825669 1104 7 7.2234858990566515 1104 8 7.02649902198435 1104 9 6.066164888895386 1104 10 4.8898249526748465 1104 11 7.835463706989753 1104 12 5.1557076285247625 1104 13 9.154800240205997 1104 14 6.958667537666262 1104 15 6.092794378343999 1104 16 5.757817997802935 1104 17 5.958776309249664 1104 18 3.858546768314813 1104 19 4.517568900072508 1104 20 3.9230461841922235 1104 21 2.8663067878021664 1104 22 4.99334083434352 1104 23 5.189601268104042 1104 24 6.405040917160612 1104 25 5.423117733005238 1105 1 2.9036127928172917 1105 2 8.921389410582773 1105 3 5.753858337731881 1105 4 5.08788568956934 1105 5 1.5240670382636878 1105 6 4.001754944383851 1105 7 5.374522059477428 1105 8 2.779510198841681 1105 9 3.3448723182182007 1105 10 3.1927309355808298 1105 11 6.41038941376161 1105 12 3.8421691669862987 1105 13 4.2800296945123755 1105 14 2.983453862452585 1105 15 3.5984169950435394 1105 16 3.887633908388075 1105 17 4.81840944534434 1105 18 2.371043809734932 1105 19 2.4167128842934176 1105 20 1.6373052242974175 1105 21 2.258605275639116 1105 22 3.7230134181699484 1105 23 1.4579076454873423 1105 24 4.375109293228988 1105 25 4.954983610717267 1106 1 6.459824488593846 1106 2 10.854287726034194 1106 3 7.65644200552487 1106 4 9.05446131622952 1106 5 8.85604358466589 1106 6 7.925874905676427 1106 7 7.836635139449857 1106 8 7.475444509026353 1106 9 6.382827980941858 1106 10 5.7015586434702 1106 11 7.939903385273933 1106 12 5.273099943091339 1106 13 4.313366817507159 1106 14 5.731723837934357 1106 15 5.919986514179566 1106 16 5.751383681449045 1106 17 6.353930911573169 1106 18 6.563606643203109 1106 19 6.3830487928339 1106 20 5.570304450501428 1106 21 5.736328235523345 1106 22 6.264384623440929 1106 23 6.734387684547293 1106 24 7.484519022611909 1106 25 6.386837125319804 1107 1 3.428405679442772 1107 2 -13.69531012919369 1107 3 -5.593011984560944 1107 4 1.284639027905012 1107 5 4.12726282436989 1107 6 4.309612150241957 1107 7 4.327001561186702 1107 8 4.926730173420946 1107 9 3.1059544762792726 1107 10 2.6036465124069714 1107 11 5.284553791241208 1107 12 2.281888661145196 1107 13 3.7827168528653203 1107 14 3.135012320556733 1107 15 3.019938037881694 1107 16 2.7904492356512804 1107 17 3.557566112662929 1107 18 -1.3152073902507517 1107 19 1.4858346162099032 1107 20 1.8005169122112363 1107 21 -1.3399550398063873 1107 22 1.4009813614552087 1107 23 -1.2495851652508954 1107 24 1.8884338746028249 1107 25 2.8126688239536044 1108 1 5.126904943746627 1108 2 10.335308763314455 1108 3 0.5800030607946702 1108 4 5.625708183342724 1108 5 7.288835630844858 1108 6 7.345324128895157 1108 7 7.281820318166314 1108 8 7.05117871796698 1108 9 5.441315811805268 1108 10 4.61069357373891 1108 11 6.849646117413204 1108 12 2.1803634374281273 1108 13 3.5792019774481254 1108 14 4.844986755907303 1108 15 4.995565279538351 1108 16 4.7687300499201974 1108 17 0.06789900197496479 1108 18 3.5587726097914683 1108 19 4.677393004307035 1108 20 5.054412090815461 1108 21 4.774326331776109 1108 22 1.5512474575791337 1108 23 4.500452516746819 1108 24 5.360387279809906 1108 25 5.640672564709906 1109 1 3.205207888858606 1109 2 -13.69531012919369 1109 3 -5.69777726998416 1109 4 1.3661995309218042 1109 5 0.7288692403528358 1109 6 2.1620788740981425 1109 7 3.294907752445308 1109 8 3.6534561450825542 1109 9 2.9896531323255395 1109 10 2.160032421554515 1109 11 0.4439090735181068 1109 12 0.8562664113740013 1109 13 1.9279150396527456 1109 14 2.7056938170576856 1109 15 2.916550948191855 1109 16 2.6317460752579827 1109 17 3.305146986981338 1109 18 3.524112466793376 1109 19 1.5761774754528695 1109 20 0.941225990554603 1109 21 0.05872449181322992 1109 22 2.2419106821996735 1109 23 2.764320493553555 1109 24 3.7998707926792825 1109 25 4.172334883765956 1110 1 2.9856236368171913 1110 2 9.121984152535738 1110 3 6.018826606240545 1110 4 6.8984801839424 1110 5 7.897098434146709 1110 6 3.429482243369602 1110 7 6.071466373254697 1110 8 6.927349789069428 1110 9 4.965436855422585 1110 10 4.847202916412645 1110 11 2.5778263290260135 1110 12 5.23397262155126 1110 13 9.638618929187867 1110 14 7.206087342408242 1110 15 6.581223945986956 1110 16 6.090663186087454 1110 17 6.646857201857756 1110 18 6.64992194265233 1110 19 5.426698607185479 1110 20 5.73640183272779 1110 21 3.7776345423349404 1110 22 5.815187417328179 1110 23 6.282541295998044 1110 24 7.084117625668284 1110 25 7.65650127554359 1111 1 4.957908872599933 1111 2 9.395051316589441 1111 3 7.2657837514181836 1111 4 7.918969393220234 1111 5 8.301852737272743 1111 6 7.245194184579347 1111 7 7.085175323420774 1111 8 7.327449490525183 1111 9 5.6368449424429095 1111 10 4.9902801066239775 1111 11 6.857463706989751 1111 12 5.963011510720211 1111 13 7.829121525346558 1111 14 5.971251205671617 1111 15 5.251514592534093 1111 16 5.02040166580829 1111 17 6.447014595728916 1111 18 6.254605670590582 1111 19 5.260522045989511 1111 20 4.657338018194903 1111 21 7.421731151925325 1111 22 6.704885680394391 1111 23 6.664243838385165 1111 24 6.286189511538122 1111 25 7.099256956566148 1112 1 -2.0992398324129553 1112 2 7.757169209649284 1112 3 6.11778403907017 1112 4 0.13400584757760825 1112 5 4.296968185290709 1112 6 3.449111783349082 1112 7 5.954141546439573 1112 8 6.268207010965067 1112 9 4.8676580018430435 1112 10 3.702249195099089 1112 11 -0.22655833157774197 1112 12 4.12257815194074 1112 13 8.130342939930514 1112 14 5.600127592762679 1112 15 4.112783359060252 1112 16 3.9750356286569293 1112 17 5.1643024800485655 1112 18 5.085610129196358 1112 19 4.66755237114689 1112 20 4.672533787498011 1112 21 -1.4095664904347487 1112 22 2.906139732415145 1112 23 4.374124684082002 1112 24 5.980445875838299 1112 25 5.90536015724766 1113 1 -18.471140658859238 1113 2 -13.69531012919369 1113 3 -7.596691436690572 1113 4 -6.11126187668698 1113 5 -1.8145577321913215 1113 6 -1.0016275852586247 1113 7 -20.503847434276683 1113 8 -9.742351552728293 1113 9 -5.723589900376645 1113 10 -4.967186541578027 1113 11 -5.626061197224807 1113 12 -3.698591222049952 1113 13 -2.5431537827825075 1113 14 -2.9022520025636247 1113 15 -3.212447000966346 1113 16 -3.6182356420438104 1113 17 -3.3976144953480336 1113 18 -4.323721430918901 1113 19 -3.5992203719581433 1113 20 -4.4604480686813375 1113 21 -1.9293790359526248 1113 22 -3.0353488208288937 1113 23 -3.422452371742704 1113 24 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2204 7 4.970117852772717 2204 8 4.84839933351083 2204 9 3.4739521608135 2204 10 3.108801365669951 2204 11 4.534366243705371 2204 12 4.7965870232733145 2204 13 3.019383239760959 2204 14 3.9971188060240728 2204 15 4.212315517640839 2204 16 4.014894099271558 2204 17 -1.4676972109995567 2204 18 2.295954868003289 2204 19 2.998426042929651 2204 20 3.3834449380863063 2204 21 5.011345506093221 2204 22 4.420142792509964 2204 23 3.290205629474759 2204 24 4.981239404031996 2204 25 4.74135360305419 2205 1 5.933980811922115 2205 2 10.2272112102679 2205 3 6.904982261369568 2205 4 7.551778150683884 2205 5 7.978894895839158 2205 6 7.444900868614681 2205 7 7.386500563096924 2205 8 7.0101650320522 2205 9 5.973616410015982 2205 10 5.369597331751233 2205 11 7.498304153476948 2205 12 6.688255669671619 2205 13 9.594937250492713 2205 14 7.081517176536913 2205 15 6.060627164511651 2205 16 5.6464260083085165 2205 17 6.349688543874311 2205 18 6.14762796770199 2205 19 4.678615518588597 2205 20 5.307623115687083 2205 21 -1.0506873028084698 2205 22 3.729048429003182 2205 23 4.9895938266507684 2205 24 6.798559411360657 2205 25 7.19412933291769 2206 1 4.093079524627001 2206 2 8.583195768578264 2206 3 6.012314654409792 2206 4 1.8845898479531016 2206 5 5.680198799547178 2206 6 6.096956849042357 2206 7 6.021054269524104 2206 8 6.062746291534374 2206 9 4.662331773998051 2206 10 3.7329791684896136 2206 11 3.652352304630643 2206 12 4.754341092919692 2206 13 8.07164864562321 2206 14 0.7785889007068896 2206 15 2.7025226439621726 2206 16 3.5878966347623056 2206 17 4.472633015234813 2206 18 -0.9462273688629921 2206 19 1.1314535658654314 2206 20 2.701024340592401 2206 21 3.7467235636868317 2206 22 -1.6905001442497536 2206 23 -2.431086368418459 2206 24 1.7419417472174068 2206 25 3.3088809483831305 2207 1 1.5086709158379357 2207 2 -13.69531012919369 2207 3 -6.012514727378113 2207 4 -4.66675348933736 2207 5 1.2173379171127827 2207 6 1.7837557885546929 2207 7 2.994133723193034 2207 8 3.1344712068205993 2207 9 0.9504564994830957 2207 10 0.6692237862324966 2207 11 -1.3989292943561438 2207 12 -2.550038445948097 2207 13 0.3856329005918191 2207 14 -5.487362072024183 2207 15 -1.5456318217457294 2207 16 -0.349382540555073 2207 17 0.7400674712057018 2207 18 0.7641062120115372 2207 19 0.10725126884774383 2207 20 -0.19018306659287987 2207 21 -3.9890929430188287 2207 22 -4.033277380102419 2207 23 -1.3420694362476873 2207 24 0.3094940351507418 2207 25 0.931207459339916 2208 1 4.956520271669763 2208 2 6.847073554681391 2208 3 6.386032752521388 2208 4 8.566022871633571 2208 5 4.096321882176127 2208 6 6.617988100843512 2208 7 6.875375000395081 2208 8 7.062426364240565 2208 9 5.485633340138218 2208 10 5.068970905511048 2208 11 6.964538276779425 2208 12 6.4821652448408855 2208 13 9.105078124208546 2208 14 3.4795725727204356 2208 15 4.980001516648652 2208 16 5.349309393596432 2208 17 6.135371592869806 2208 18 0.8902943782574511 2208 19 3.570355388281559 2208 20 4.620389715103634 2208 21 7.1717586679806224 2208 22 6.208412854738675 2208 23 2.1871576734577687 2208 24 3.198531038256853 2208 25 4.730161710060685 2209 1 3.005539725228555 2209 2 4.959696729516875 2209 3 5.188635652851076 2209 4 0.7308837171827527 2209 5 4.900857811065858 2209 6 5.304427000617164 2209 7 5.791762990963374 2209 8 4.322342643383529 2209 9 4.136241706453685 2209 10 3.300359932098715 2209 11 -0.5979546743545576 2209 12 3.071883211515157 2209 13 2.336591735404898 2209 14 3.5523575239488387 2209 15 3.873930180813133 2209 16 3.393689054044362 2209 17 1.033533510895758 2209 18 3.005312200413581 2209 19 3.5249790509641414 2209 20 3.5535153199946947 2209 21 5.806380861876239 2209 22 4.705505443397019 2209 23 0.09139276330294166 2209 24 -3.6038397412756034 2209 25 -0.21949955094537899 2210 1 -2.7803831883936354 2210 2 -13.69531012919369 2210 3 -7.747622665539895 2210 4 -2.6680816136797283 2210 5 -1.2638522880637204 2210 6 -1.765000404292703 2210 7 -1.6171205406005171 2210 8 -20.576834311348982 2210 9 -13.11424326491513 2210 10 -7.5133624459816275 2210 11 -10.362133235331305 2210 12 -5.141277314722863 2210 13 -5.436281992826201 2210 14 -4.371909251770848 2210 15 -6.286773840326376 2210 16 -5.7942667832950505 2210 17 -5.251542430615981 2210 18 -5.5061240679140475 2210 19 -10.532547847668978 2210 20 -7.645293866769091 2210 21 -5.67318313575586 2210 22 -9.865481495978463 2210 23 -8.121717240119263 2210 24 -10.8385777995408 2210 25 -8.726028433313965 2211 1 -0.42906492253385053 2211 2 4.313105018071386 2211 3 0.1376714893732789 2211 4 0.9752346599909174 2211 5 0.4540219564589769 2211 6 -0.6856812573473121 2211 7 -20.503847434276683 2211 8 -13.123818883578997 2211 9 -9.42400528408264 2211 10 -7.594756627502804 2211 11 -9.747024371551623 2211 12 -5.434487789895062 2211 13 -6.874388165591105 2211 14 -5.1688219433996565 2211 15 -5.62138607981402 2211 16 -11.576894484665509 2211 17 -8.038924485514244 2211 18 -6.3176234046632125 2211 19 -9.229283834850353 2211 20 -7.2522263730775265 2211 21 -5.055912006026727 2211 22 -6.541774640177284 2211 23 -8.788591345266694 2211 24 -21.667625749506055 2211 25 -21.43554893366143 2212 1 -0.3907468934777789 2212 2 -13.69531012919369 2212 3 -7.356414091086643 2212 4 -19.158005171706137 2212 5 -19.624519418015076 2212 6 -20.542623753841 2212 7 -9.448064005236485 2212 8 -8.996030373695167 2212 9 -4.293491113292198 2212 10 -4.837208134555123 2212 11 -4.90627706413412 2212 12 -2.1217777419975263 2212 13 -5.718522702096436 2212 14 -3.124761502530621 2212 15 -3.083173901666939 2212 16 -3.1670794591289777 2212 17 -3.261859731220664 2212 18 -2.3085202264896694 2212 19 -2.7978355297880313 2212 20 -3.010780449660661 2212 21 -0.9424266002208022 2212 22 -2.2982249299441477 2212 23 -2.252893126208239 2212 24 -1.227100728997442 2212 25 -1.5979771535137672 2213 1 -18.471140658859238 2213 2 -13.69531012919369 2213 3 -8.434771881667569 2213 4 -5.836769650470615 2213 5 -19.624519418015076 2213 6 -10.525635336852583 2213 7 -20.503847434276683 2213 8 -10.439382573897245 2213 9 -8.225189036458538 2213 10 -7.196867454017557 2213 11 -6.66299961347357 2213 12 -5.221646296829164 2213 13 -18.91786642646067 2213 14 -12.179942500943778 2213 15 -6.932786059236438 2213 16 -6.284846094861159 2213 17 -4.829159340685983 2213 18 -4.738884377116332 2213 19 -5.616631872128263 2213 20 -5.633767200621161 2213 21 -3.715955760460382 2213 22 -4.512213863211176 2213 23 -4.2252275607247896 2213 24 -3.4351933170736224 2213 25 -4.228599126711622 2214 1 -18.471140658859238 2214 2 -13.69531012919369 2214 3 -12.18603505784424 2214 4 -19.158005171706137 2214 5 -19.624519418015076 2214 6 -20.542623753841 2214 7 -16.436373385833775 2214 8 -14.892578255985661 2214 9 -13.90369900960519 2214 10 -13.947902844326306 2214 11 -14.619179891626166 2214 12 -12.548945197311456 2214 13 -18.91786642646067 2214 14 -16.72368540351021 2214 15 -23.428538954989335 2214 16 -18.153773697698796 2214 17 -23.112557024083667 2214 18 -17.68313027897469 2214 19 -14.98093629023891 2214 20 -13.804893838875826 2214 21 -11.33084200112517 2214 22 -12.350010953430413 2214 23 -12.263411419323871 2214 24 -11.160705334281142 2214 25 -10.894026442311949 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 157511.0 3 9.0 4 667.0 5 2750.0 6 4004.0 7 3892.0 8 3325.0 9 3270.0 10 2950.0 11 2823.0 12 2680.0 13 2864.0 14 4199.0 15 8562.0 16 12351.0 17 12757.0 18 13209.0 19 15627.0 20 18390.0 21 21823.0 22 22827.0 23 24997.0 24 26124.0 25 27068.0 26 22425.0 27 18058.0 28 19761.0 29 22468.0 30 21945.0 31 24944.0 32 32450.0 33 40100.0 34 48335.0 35 54475.0 36 53256.0 37 54970.0 38 37101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83629759434033 18.962266583957835 12.605314630640367 25.59612119106147 2 13.57719528130071 20.28180610255213 42.38669589971569 23.75430271643146 3 18.58887737789589 25.137005840577753 29.144958435305718 27.12915834622064 4 11.690759497820173 17.06548223904896 38.98572458779952 32.25803367533134 5 12.371517515432833 37.567728648250906 36.2870785075328 13.773675328783472 6 31.5235057298407 37.75513864476092 18.23822230071109 12.483133324687294 7 27.43245125076298 31.314560284029458 23.045507964251644 18.20748050095592 8 24.239164417561522 37.72090678815025 20.485934319749376 17.553994474538854 9 25.736854389729242 15.95944205576362 20.28359282345233 38.020110731054814 10 13.742813271721657 30.072668642754323 35.19981175544055 20.984706330083476 11 32.974937305722825 22.803322671552063 24.830687910140707 19.391052112584404 12 22.876027667860257 25.268306754544938 32.177244878122195 19.678420699472603 13 27.811204425220616 21.69286567817291 25.648102955944147 24.847826940662323 14 20.010363454276366 22.681898459788716 28.285858213037514 29.021879872897404 15 22.11150134904251 31.628648934536706 23.88572988439912 22.374119832021663 16 21.298079736292884 29.032651309758332 28.01717412081375 21.65209483313503 17 20.883118275378138 28.844363987213473 29.006144745049117 21.26637299235927 18 20.711895966603596 28.296242904766196 30.58197435204373 20.40988677658648 19 22.133399290163627 27.616020786006906 27.906462968921847 22.34411695490762 20 22.290220580555108 28.244613675424684 28.79867632675928 20.666489417260927 21 21.641944049898104 26.823812758599395 27.35762366454641 24.17661952695609 22 21.683027698908905 28.553939695676384 28.581959692092706 21.181072913322005 23 21.56931742381428 28.228614623061222 28.52374876040108 21.67831919272342 24 21.645818559892554 27.864859153742742 28.92530591555577 21.564016370808933 25 21.97226955077241 27.846446962139005 28.597410845254995 21.583872641833594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 937.0 1 937.0 2 1892.0 3 2847.0 4 2847.0 5 2847.0 6 4112.5 7 5378.0 8 5378.0 9 5378.0 10 6378.0 11 7378.0 12 7378.0 13 7378.0 14 9264.5 15 11151.0 16 11151.0 17 11151.0 18 15752.0 19 20353.0 20 20353.0 21 20353.0 22 28014.0 23 35675.0 24 35675.0 25 35675.0 26 45688.0 27 55701.0 28 55701.0 29 55701.0 30 66945.5 31 78190.0 32 78190.0 33 78190.0 34 85622.5 35 93055.0 36 93055.0 37 93055.0 38 97541.0 39 102027.0 40 102027.0 41 102027.0 42 103202.5 43 104378.0 44 104378.0 45 104378.0 46 102094.5 47 99811.0 48 99811.0 49 99811.0 50 92000.0 51 84189.0 52 84189.0 53 84189.0 54 72273.0 55 60357.0 56 60357.0 57 60357.0 58 50181.0 59 40005.0 60 40005.0 61 40005.0 62 31706.5 63 23408.0 64 23408.0 65 23408.0 66 17718.0 67 12028.0 68 12028.0 69 12028.0 70 8572.5 71 5117.0 72 5117.0 73 5117.0 74 3535.0 75 1953.0 76 1953.0 77 1953.0 78 1330.5 79 708.0 80 708.0 81 708.0 82 471.5 83 235.0 84 235.0 85 235.0 86 150.0 87 65.0 88 65.0 89 65.0 90 41.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.81067781345307 2 35.60304721959556 3 5.412164025340634 4 13.349515424862746 5 12.827483203486054 6 14.221265445869482 7 9.06496940117188 8 7.257324842271946 9 0.11124694810566566 10 1.421120588141312 11 5.426365763396677 12 5.639510182054447 13 15.617532992412722 14 1.5619544905303995 15 2.8859115208049544 16 2.9188122139681196 17 2.8525374363732547 18 1.4843183224907008 19 1.4318902395004776 20 0.29149067360027076 21 4.17968985771042 22 1.5879910102998103 23 0.11172033937420041 24 8.709689254136551 25 0.11657259987668159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 844967.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.17437216031882 #Duplication Level Percentage of deduplicated Percentage of total 1 97.16614504631974 91.50560704975635 2 2.1746178237849434 4.0958653648717185 3 0.33709624426982115 0.9523748148513564 4 0.11092943132228252 0.41786838195508635 5 0.0586379412848114 0.27610956526353764 6 0.03608985014590105 0.2039243387310133 7 0.02040962063985764 0.1345444246852214 8 0.015335792581631442 0.11553909103648127 9 0.012358768061206723 0.10474913005552294 >10 0.059009884324523726 1.0193377567340707 >50 0.00500623828509722 0.32831712720209744 >100 0.004235024917487233 0.7789285926724385 >500 1.2833406268661368E-4 0.06683436218503047 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.1834781713368688E-4 0.0 8 0.0 0.0 0.0 1.1834781713368688E-4 0.0 9 0.0 0.0 0.0 1.1834781713368688E-4 0.0 10 0.0 0.0 0.0 1.1834781713368688E-4 0.0 11 0.0 0.0 0.0 1.1834781713368688E-4 0.0 12 0.0 0.0 0.0 1.1834781713368688E-4 0.0 13 0.0 0.0 0.0 1.1834781713368688E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTAAG 50 0.0024999587 15.941104 1 GAACTAG 40 0.0029862737 15.522776 6 GTCCTAA 190 0.0 14.682594 1 TGTAGGA 450 0.0 14.496418 2 ATTAGAC 45 0.0047311657 14.48514 3 CTAACCA 45 0.00532603 14.228999 4 GTCCTAC 290 0.0 13.742329 1 TACTATA 110 1.2754299E-6 13.590392 2 CTGTAGG 485 0.0 13.421204 1 GTCTAGG 70 0.0015103868 13.284253 1 TCTAACC 75 5.918018E-5 13.036626 3 TATAGGA 85 3.7598272E-4 12.790957 2 TAGGACC 210 0.0 12.704462 4 GTCTTAA 95 8.226877E-5 12.585081 1 CCATGTA 195 3.274181E-11 12.544978 2 GTAGGAC 445 0.0 12.450709 3 TCTACAC 115 1.5455043E-7 12.280878 3 GTGCTGG 130 7.9680103E-7 12.262387 1 GTCCTAT 110 2.4045374E-5 12.076593 1 TCTAGGA 115 3.0014005E-5 11.817732 2 >>END_MODULE