FastQCFastQC Report
Thu 26 May 2016
SRR1926634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844967
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15660.18533268163135364No Hit
CTGTAGGACGTGGAATATGGCAAGA12760.15101181466258445No Hit
GTCCTACAGTGGACATTTCTAAATT11470.13574494625233885No Hit
GGTATCAACGCAGAGTACTTTTTTT9330.11041851338572986No Hit
GTCCTAAAGTGTGTATTTCTCATTT9270.10970842648292774No Hit
CTTTAGGACGTGAAATATGGCGAGG9180.10864329612872456No Hit
GTACATGGGAAGCAGTGGTATCAAC8750.10355433999197601No Hit
GTCCTACAGTGTGCATTTCTCATTT8590.10166077491783702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG350.002118419916.3434355
TAGGACC2950.012.2813914
CCAACGA701.116901E-412.18214119
TCCAACG951.0706899E-611.96912818
GGACCTG5200.011.9163796
GTAGGAC7100.011.8191963
GGACCTA657.7861134E-411.733056
CTTTTAG657.8016886E-411.7302571
AGGACCT4800.011.7184715
GGCGAGG2600.011.66153719
TGTAGGA7700.011.6426672
ACTGTTC2500.011.4023868
GACGTGG3100.011.3383917
GTCCTAT1354.478352E-911.2958021
AATCCCG1354.9094524E-911.22962819
AGATAGT855.13081E-511.2154156
CTGTAGG7900.011.0991981
TAGGACA2700.010.9467174
GTCCCAA700.001450421510.8923811
GGACGTG6450.010.7894036
Produced by FastQC (version 0.11.5)