FastQCFastQC Report
Thu 26 May 2016
SRR1926633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888330
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG250.007379301418.3463279
GTCCTAA2000.015.2035881
TACTACA902.5105928E-615.0318822
CTGTAGG4650.014.4999161
GTCTAGG1202.0630978E-814.3222211
TGTAGGA5200.014.30919552
TAGGACC1950.014.2149394
GTTCAGG1151.9058825E-713.7953161
GTCCTAG1253.527748E-813.7493311
GTCCTAC4250.013.6871181
GAGTTCG450.007541788313.5013557
GTAGGAC5150.013.4690443
GTACAAG1401.089029E-813.2205111
GTGTAAC600.00866234913.2205111
TAGGACA2300.012.9788594
TCCTACA5200.012.2278582
CTAGACT801.290629E-411.9938554
AGGACCT3450.011.984865
GTACTGG1001.3893475E-411.898461
AGTACTA707.347765E-411.8136495
Produced by FastQC (version 0.11.5)