##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888330 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.24999831143832 27.0 7.0 31.0 2.0 31.0 2 16.567784494500916 20.0 2.0 30.0 2.0 31.0 3 19.930126191843122 24.0 15.0 31.0 2.0 31.0 4 22.02300383866356 28.0 2.0 35.0 2.0 37.0 5 22.618390688145173 29.0 2.0 35.0 2.0 37.0 6 23.559314669098196 31.0 2.0 35.0 2.0 37.0 7 23.405290826607228 31.0 7.0 35.0 2.0 37.0 8 23.476759762700798 31.0 8.0 35.0 2.0 37.0 9 26.14605157993088 32.0 17.0 37.0 2.0 39.0 10 26.83657199464163 32.0 23.0 37.0 2.0 39.0 11 23.997004491574078 30.0 7.0 37.0 2.0 39.0 12 25.11543120236849 31.0 10.0 37.0 2.0 39.0 13 21.844487971812278 27.0 2.0 35.0 2.0 39.0 14 25.093987594700167 31.0 16.0 38.0 2.0 40.0 15 26.15059831368973 31.0 17.0 38.0 2.0 40.0 16 26.515866851282745 31.0 17.0 38.0 2.0 40.0 17 25.802655544673712 31.0 10.0 38.0 2.0 40.0 18 25.826746817061228 31.0 11.0 38.0 2.0 40.0 19 26.24273411907737 31.0 16.0 39.0 2.0 40.0 20 26.50066867042653 31.0 11.0 39.0 2.0 40.0 21 24.347665844899982 30.0 2.0 37.0 2.0 40.0 22 25.28791665259532 31.0 2.0 38.0 2.0 40.0 23 25.267786745916496 31.0 2.0 38.0 2.0 40.0 24 24.38687199576734 31.0 2.0 38.0 2.0 40.0 25 24.169952607702093 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.455025933504899 1101 2 10.25642363481137 1101 3 6.757147203353004 1101 4 8.215548188949523 1101 5 7.988890051769243 1101 6 7.584541975793304 1101 7 7.260755885386022 1101 8 5.954118756431175 1101 9 5.946037148121292 1101 10 5.577568631769267 1101 11 8.336328406571052 1101 12 5.720571981970654 1101 13 10.249549512725885 1101 14 7.460273933085034 1101 15 6.935525232179284 1101 16 6.209868541227635 1101 17 2.4631047079546455 1101 18 4.7816403508135465 1101 19 5.454923370094015 1101 20 3.7770138905580417 1101 21 0.6808395240261227 1101 22 4.496398002200586 1101 23 5.663029652537453 1101 24 6.885783185494354 1101 25 7.501308154556547 1102 1 6.127694788964305 1102 2 11.143291279745306 1102 3 7.148059833596875 1102 4 8.449071235492138 1102 5 8.438628359923431 1102 6 7.3248906841915264 1102 7 7.288684265304784 1102 8 7.440082922308484 1102 9 6.367365033554851 1102 10 5.700844756803384 1102 11 8.45411020694673 1102 12 6.10331958580452 1102 13 10.446929383324889 1102 14 7.955510900670568 1102 15 5.750235765677445 1102 16 6.132096694774923 1102 17 7.072608296183329 1102 18 7.035855516096834 1102 19 5.822899031146882 1102 20 6.1938504696194805 1102 21 5.366011791112353 1102 22 6.38895343110228 1102 23 6.728186908465659 1102 24 8.14001520822783 1102 25 7.797175269684903 1103 1 4.576257089283793 1103 2 8.58354687079962 1103 3 5.7180278847150845 1103 4 7.234374749869136 1103 5 6.560673088357937 1103 6 6.402206978121239 1103 7 6.360249760512453 1103 8 5.645833721030531 1103 9 4.7041062476123585 1103 10 3.668895875014247 1103 11 4.768487203751842 1103 12 3.381274857370016 1103 13 7.556194559043739 1103 14 5.901836779264816 1103 15 5.031385925421855 1103 16 4.666920975925084 1103 17 2.4310747498574514 1103 18 1.4032676566719111 1103 19 3.2005348138944854 1103 20 3.5523169232936027 1103 21 5.7934878294510135 1103 22 1.445183463051162 1103 23 3.438091061820291 1103 24 4.972603067652749 1103 25 1.2086768671289931 1104 1 5.3602917427491406 1104 2 9.86770093716472 1104 3 6.551067298880142 1104 4 7.0432634981688835 1104 5 6.623741288149162 1104 6 6.429002521094066 1104 7 6.534018858668276 1104 8 6.4485307918558235 1104 9 5.3374683138509305 1104 10 4.1389398442674015 1104 11 7.262577846841712 1104 12 4.105472972069663 1104 13 8.508405674723996 1104 14 5.967743210163498 1104 15 5.2838354714946725 1104 16 4.989894910698752 1104 17 5.2364143253485445 1104 18 3.0842549087517526 1104 19 3.8585224297487244 1104 20 3.2810159553373808 1104 21 2.2605729684760973 1104 22 4.517726799653193 1104 23 4.843051046636663 1104 24 5.685082901898156 1104 25 5.0815298073750945 1105 1 3.473984362011066 1105 2 9.520559973068309 1105 3 6.1304689890491595 1105 4 5.389035575376607 1105 5 2.213444008421188 1105 6 4.954355056347591 1105 7 5.911094066189026 1105 8 2.6902362123978776 1105 9 3.716543721391684 1105 10 3.4693666369466705 1105 11 6.80038162721975 1105 12 4.09241541631409 1105 13 4.982859370093532 1105 14 3.783281013943874 1105 15 3.986899527900313 1105 16 4.261315802787962 1105 17 4.987795713487358 1105 18 2.559202403501228 1105 19 2.342083285834331 1105 20 1.5149205958014278 1105 21 2.392361147293979 1105 22 3.9887238993631655 1105 23 1.3861116026922673 1105 24 4.468554999107877 1105 25 5.2343388382781875 1106 1 6.703720143470985 1106 2 11.342896640232563 1106 3 7.822192256203461 1106 4 9.266523508899024 1106 5 9.168832250447362 1106 6 8.141108676290866 1106 7 7.7000768745683885 1106 8 7.331557056304927 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3.0296935222884827 2207 8 3.389400936562602 2207 9 0.8299664098143751 2207 10 0.6675892476692802 2207 11 -1.1859528716147487 2207 12 -2.1191716077729374 2207 13 0.5125310872652484 2207 14 -5.48871782055496 2207 15 -1.325046859046072 2207 16 -0.3395097605376023 2207 17 0.8056958688875149 2207 18 0.8628154571142836 2207 19 0.31008056633161374 2207 20 0.09264009852093125 2207 21 -3.8376213701885398 2207 22 -4.095668097528833 2207 23 -1.2232221191414538 2207 24 0.5443999874528629 2207 25 1.2920020084413544 2208 1 4.635788057413677 2208 2 6.221183921512463 2208 3 6.035464904305147 2208 4 7.946825592201499 2208 5 3.285549990065654 2208 6 5.781200252141975 2208 7 6.178101558429532 2208 8 6.267655018620051 2208 9 4.86002908616388 2208 10 3.980592470143339 2208 11 6.358481036829982 2208 12 5.84264053278244 2208 13 8.075168890432217 2208 14 2.468642217683012 2208 15 4.099028028784076 2208 16 4.425445448614841 2208 17 5.287830411034083 2208 18 -0.027119322477744845 2208 19 2.5996503120799517 2208 20 3.8221176414230094 2208 21 6.4132081470570945 2208 22 5.5015137795237585 2208 23 1.446576823146966 2208 24 3.0250007707145024 2208 25 4.287119728964971 2209 1 2.5113887527391547 2209 2 4.229666938678168 2209 3 4.629414367691069 2209 4 -0.010355736586959097 2209 5 4.248064052795566 2209 6 4.889891781985472 2209 7 5.472601481381414 2209 8 4.228713470528316 2209 9 3.994759404231644 2209 10 2.945285507735484 2209 11 -0.672413520792162 2209 12 2.7693837995685975 2209 13 1.9929421341502866 2209 14 3.0310440489178916 2209 15 3.52280774299928 2209 16 3.163484162687535 2209 17 0.7418353876859918 2209 18 2.7710462757497254 2209 19 3.365741106818742 2209 20 3.4643571579547547 2209 21 5.5975671086415595 2209 22 4.389585842493894 2209 23 -0.02782910496231139 2209 24 -3.103308870805126 2209 25 -0.3251834072989723 2210 1 -2.3431519100446145 2210 2 -13.667571340063002 2210 3 -7.551432717743413 2210 4 -2.454631959610605 2210 5 -0.6783843659877782 2210 6 -1.1843680545464395 2210 7 -0.905800958605596 2210 8 -20.54713752497586 2210 9 -12.850496730135 2210 10 -6.83532356595542 2210 11 -9.919278455813403 2210 12 -4.467473686263059 2210 13 -5.286215274946526 2210 14 -3.9113470158503745 2210 15 -5.528140055305823 2210 16 -4.965104291370547 2210 17 -4.4966512579055475 2210 18 -4.631656826814012 2210 19 -9.845705023721942 2210 20 -7.079689033059374 2210 21 -5.161153770181652 2210 22 -9.355267983874857 2210 23 -7.3884087010842485 2210 24 -9.861078220258387 2210 25 -7.707773266652925 2211 1 -0.08730053896404044 2211 2 4.654880358804419 2211 3 0.5709459606303966 2211 4 1.4160116990382718 2211 5 0.7746250224253188 2211 6 -0.16967715450174126 2211 7 -20.506203913608953 2211 8 -13.333946319113101 2211 9 -9.483518190195875 2211 10 -7.786327799085875 2211 11 -9.815030068013732 2211 12 -5.212805766197235 2211 13 -6.822134691104726 2211 14 -5.221144066305655 2211 15 -5.610086319918313 2211 16 -11.579454071168122 2211 17 -7.99675036353873 2211 18 -6.354193716257317 2211 19 -8.916847208314351 2211 20 -7.258227271950037 2211 21 -4.84767705955802 2211 22 -6.274685931793169 2211 23 -8.045254439474906 2211 24 -21.628035909983033 2211 25 -21.388767222327875 2212 1 -0.5592362833229814 2212 2 -13.667571340063002 2212 3 -7.415352815148934 2212 4 -19.134954323613293 2212 5 -19.597793365316186 2212 6 -20.51344797395544 2212 7 -9.688089038793047 2212 8 -9.145075669305756 2212 9 -4.697843151758075 2212 10 -5.011507085550765 2212 11 -4.906297647114165 2212 12 -2.425230668986636 2212 13 -5.725809609838334 2212 14 -3.375466810396734 2212 15 -3.316666322057287 2212 16 -3.524921942857002 2212 17 -3.531568584871785 2212 18 -2.6940555069731786 2212 19 -3.2883437756494303 2212 20 -3.3925785488111195 2212 21 -1.336671632640865 2212 22 -2.7497776477724045 2212 23 -2.910546993579729 2212 24 -1.7539563812643308 2212 25 -2.335748076525224 2213 1 -18.423742910716207 2213 2 -13.667571340063002 2213 3 -8.238051744603153 2213 4 -5.470480639402767 2213 5 -19.597793365316186 2213 6 -10.384793003195206 2213 7 -20.506203913608953 2213 8 -10.336611209186385 2213 9 -8.132539213217566 2213 10 -6.7155416565142545 2213 11 -6.340799925146015 2213 12 -4.79916486013461 2213 13 -18.820802246068084 2213 14 -11.957116381058784 2213 15 -6.582345554502663 2213 16 -5.934291586898375 2213 17 -4.48565590799058 2213 18 -4.453423859125035 2213 19 -5.265605443564922 2213 20 -5.309507899679698 2213 21 -3.487722584763439 2213 22 -4.347433729557622 2213 23 -4.091991799753238 2213 24 -3.1382698281116888 2213 25 -4.153387105368811 2214 1 -18.423742910716207 2214 2 -13.667571340063002 2214 3 -11.785676109876887 2214 4 -19.134954323613293 2214 5 -19.597793365316186 2214 6 -20.51344797395544 2214 7 -16.08126976084337 2214 8 -14.398761756758125 2214 9 -13.157065061786897 2214 10 -13.031250265173744 2214 11 -14.019870388104698 2214 12 -11.692070687037248 2214 13 -18.820802246068084 2214 14 -16.15996565378699 2214 15 -23.361585320584535 2214 16 -17.60289644701472 2214 17 -23.050714387522742 2214 18 -17.174768118221706 2214 19 -14.248503774408128 2214 20 -12.876801719538095 2214 21 -10.466025728487542 2214 22 -11.438804789633918 2214 23 -11.465383484261555 2214 24 -10.337430115426406 2214 25 -9.979636405822168 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 162215.0 3 12.0 4 680.0 5 2901.0 6 4173.0 7 4123.0 8 3530.0 9 3371.0 10 3151.0 11 2987.0 12 2752.0 13 2922.0 14 4443.0 15 8931.0 16 12819.0 17 13172.0 18 13552.0 19 15918.0 20 19066.0 21 22679.0 22 24042.0 23 26165.0 24 27668.0 25 28239.0 26 24283.0 27 19265.0 28 20873.0 29 23968.0 30 22860.0 31 26407.0 32 33672.0 33 42481.0 34 51140.0 35 57633.0 36 56467.0 37 58603.0 38 41167.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.676541146799806 19.2084610574015 12.763991552270326 25.351006243528364 2 13.531994330556227 20.26031043661631 42.315138106267504 23.89255712655996 3 18.498099331954545 25.16778086499042 29.143024234553444 27.191095568501595 4 11.747410357537733 17.103445072744794 38.886889164979806 32.262255404737665 5 12.37821421964605 37.64298267250416 36.19324371544264 13.785559392407146 6 31.44893550089564 37.865568153161 18.174089605632265 12.511406740311095 7 27.311905300404014 31.459461864453914 23.074993171652906 18.15363966348917 8 24.352879475884155 37.585980942783536 20.391124719755723 17.670014861576586 9 25.807465875691953 15.969680304368994 20.343519536955228 37.87933428298383 10 13.937606280402465 29.90895787079039 35.04210185594646 21.111333992860693 11 32.969541944957605 22.87705461607693 24.826630666477193 19.326772772488276 12 22.948651649144114 25.221087789881203 32.05701787679811 19.773242684176566 13 27.654241467107166 21.80941281131744 25.59795255448921 24.93839316708618 14 19.99025283284179 22.693094348925758 28.31905879345136 28.997594024781087 15 22.11640272800832 31.476707895041034 23.974107039648594 22.432782337302047 16 21.46339546399188 28.860357434295846 28.065596660062926 21.610650441649348 17 20.82622284100034 28.748649632295596 29.013350587229418 21.411776939474642 18 20.711587677400814 28.20359295095503 30.595282084969917 20.48953728667424 19 22.207138509516415 27.607867542965785 27.792633525052263 22.392360422465536 20 22.480644658738637 28.159001673060118 28.67649881124676 20.68385485695449 21 21.697468369245897 26.746684769250578 27.27864324254731 24.277203618956214 22 21.841995917938696 28.48390690708516 28.52443072595853 21.149666449017612 23 21.54047968408739 27.953735571949235 28.661586279799938 21.84419846416344 24 21.762206501774084 27.842282432030373 28.793254796662804 21.602256269532738 25 22.022767716770595 27.68911393888713 28.66092871818048 21.6271896261618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 906.0 1 906.0 2 1779.0 3 2652.0 4 2652.0 5 2652.0 6 3834.0 7 5016.0 8 5016.0 9 5016.0 10 6166.5 11 7317.0 12 7317.0 13 7317.0 14 9350.5 15 11384.0 16 11384.0 17 11384.0 18 16187.5 19 20991.0 20 20991.0 21 20991.0 22 29189.5 23 37388.0 24 37388.0 25 37388.0 26 47709.0 27 58030.0 28 58030.0 29 58030.0 30 69295.5 31 80561.0 32 80561.0 33 80561.0 34 89134.0 35 97707.0 36 97707.0 37 97707.0 38 102361.5 39 107016.0 40 107016.0 41 107016.0 42 108101.0 43 109186.0 44 109186.0 45 109186.0 46 107245.0 47 105304.0 48 105304.0 49 105304.0 50 97289.0 51 89274.0 52 89274.0 53 89274.0 54 77367.0 55 65460.0 56 65460.0 57 65460.0 58 54163.0 59 42866.0 60 42866.0 61 42866.0 62 34113.5 63 25361.0 64 25361.0 65 25361.0 66 19221.5 67 13082.0 68 13082.0 69 13082.0 70 9356.5 71 5631.0 72 5631.0 73 5631.0 74 3883.0 75 2135.0 76 2135.0 77 2135.0 78 1445.5 79 756.0 80 756.0 81 756.0 82 493.5 83 231.0 84 231.0 85 231.0 86 142.0 87 53.0 88 53.0 89 53.0 90 35.5 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.157869260297412 2 34.87330158837369 3 4.9111253700764355 4 12.745601296815373 5 12.373104589510655 6 13.401326083775173 7 8.915943399412381 8 7.059426114169284 9 0.11099478797293799 10 1.2308488962435131 11 4.940731484921145 12 5.091126045501109 13 14.71333851158916 14 1.3708869451667736 15 2.6150191933177984 16 2.607589521911902 17 2.5694280278725246 18 1.2947890986457735 19 1.2387288507649186 20 0.28457892900160975 21 3.820652235092815 22 1.3848457217475487 23 0.11212049576171017 24 8.437405018405322 25 0.11493476523364064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 888330.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.60321605575146 #Duplication Level Percentage of deduplicated Percentage of total 1 96.87002291405355 90.67345684149748 2 2.403266119721366 4.499068756874929 3 0.356953376504594 1.002359520683685 4 0.13349409828234418 0.4998190769475993 5 0.060351941230492256 0.2824567897190891 6 0.041332687150017956 0.23213234672807476 7 0.0279524922878527 0.18315102224416216 8 0.015127324080973741 0.11327729474374057 9 0.012710377230972783 0.10707589674907644 >10 0.06906413430195474 1.1525729215691525 >50 0.0051708226996625235 0.34189558784254204 >100 0.004184492700961953 0.7083682188231385 >500 3.692197552613467E-4 0.20436572557728283 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.1257077887721906E-4 0.0 0.0 0.0 5 0.0 1.1257077887721906E-4 0.0 0.0 0.0 6 0.0 1.1257077887721906E-4 0.0 0.0 0.0 7 0.0 1.1257077887721906E-4 0.0 0.0 0.0 8 0.0 1.1257077887721906E-4 0.0 0.0 0.0 9 0.0 1.1257077887721906E-4 0.0 1.1257077887721906E-4 0.0 10 0.0 1.1257077887721906E-4 0.0 1.1257077887721906E-4 0.0 11 0.0 1.1257077887721906E-4 0.0 1.1257077887721906E-4 0.0 12 0.0 1.1257077887721906E-4 0.0 1.1257077887721906E-4 3.3771233663165717E-4 13 0.0 1.1257077887721906E-4 0.0 1.1257077887721906E-4 3.3771233663165717E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTCCG 25 0.0073793014 18.346327 9 GTCCTAA 200 0.0 15.203588 1 TACTACA 90 2.5105928E-6 15.031882 2 CTGTAGG 465 0.0 14.499916 1 GTCTAGG 120 2.0630978E-8 14.322221 1 TGTAGGA 520 0.0 14.3091955 2 TAGGACC 195 0.0 14.214939 4 GTTCAGG 115 1.9058825E-7 13.795316 1 GTCCTAG 125 3.527748E-8 13.749331 1 GTCCTAC 425 0.0 13.687118 1 GAGTTCG 45 0.0075417883 13.501355 7 GTAGGAC 515 0.0 13.469044 3 GTACAAG 140 1.089029E-8 13.220511 1 GTGTAAC 60 0.008662349 13.220511 1 TAGGACA 230 0.0 12.978859 4 TCCTACA 520 0.0 12.227858 2 CTAGACT 80 1.290629E-4 11.993855 4 AGGACCT 345 0.0 11.98486 5 GTACTGG 100 1.3893475E-4 11.89846 1 AGTACTA 70 7.347765E-4 11.813649 5 >>END_MODULE