FastQCFastQC Report
Thu 26 May 2016
SRR1926633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888330
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17440.19632343836187No Hit
CTGTAGGACGTGGAATATGGCAAGA13640.15354654238852677No Hit
GTCCTACAGTGGACATTTCTAAATT13200.14859342811792914No Hit
GGTATCAACGCAGAGTACTTTTTTT10770.1212387288507649No Hit
CTTTAGGACGTGAAATATGGCGAGG10230.11515990679139508No Hit
GTCCTAAAGTGTGTATTTCTCATTT9870.1111073587518152No Hit
CCCATGTACTCTGCGTTGATACCAC9230.10390282890367318No Hit
GTACATGGGAAGCAGTGGTATCAAC9040.10176398410500602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4200.014.5221594
TGTAGGA9350.013.66284752
GGCGTGC500.001498593713.3000798
CTGAGCG500.001498593713.3000799
GTAGGAC8900.013.279493
CTGTAGG10050.012.6127771
CCCCTAT701.0575283E-412.2537671
AGGACCT7000.011.9807825
TTAGCCT657.8069686E-411.7294374
TGGCGAG8550.011.63981118
GGACCTG6800.011.6317526
GTCCTAG1251.7416824E-811.4368491
GGCGAGA5650.011.23892119
TGGAATA11550.011.1679411
GGCGAGG3150.011.13241619
CGCCTTT600.005973889511.05781916
GTCCTAA3450.011.0500961
ATGGCGA9800.010.92886517
GTTCTAA1053.3288525E-610.8922371
TTGTACC700.001451395510.891624
Produced by FastQC (version 0.11.5)