##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888330 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.178951515765537 31.0 28.0 31.0 18.0 34.0 2 28.67817027456013 31.0 28.0 33.0 22.0 34.0 3 29.03466617135524 31.0 28.0 33.0 24.0 34.0 4 32.81884547409184 35.0 33.0 37.0 27.0 37.0 5 32.514160278274964 35.0 33.0 37.0 25.0 37.0 6 32.60007204529848 35.0 33.0 37.0 26.0 37.0 7 32.43971384508009 35.0 33.0 37.0 24.0 37.0 8 32.40572197269033 35.0 33.0 37.0 24.0 37.0 9 33.62157418977182 37.0 33.0 39.0 22.0 39.0 10 33.51087321153175 37.0 33.0 39.0 22.0 39.0 11 33.746155145047446 37.0 34.0 39.0 25.0 39.0 12 33.56525728051513 37.0 33.0 39.0 22.0 39.0 13 33.67992300158725 37.0 34.0 39.0 23.0 39.0 14 34.33249017819954 38.0 33.0 40.0 19.0 41.0 15 34.450638839170125 38.0 34.0 40.0 22.0 41.0 16 34.41918881496741 38.0 34.0 40.0 22.0 41.0 17 34.33370031407247 38.0 33.0 40.0 19.0 41.0 18 34.29867841905598 38.0 33.0 40.0 19.0 41.0 19 34.34153974311348 39.0 34.0 40.0 18.0 41.0 20 34.32836558486148 39.0 34.0 40.0 18.0 41.0 21 34.258335303321964 39.0 34.0 40.0 18.0 41.0 22 34.17046705616156 39.0 34.0 40.0 18.0 41.0 23 33.83566467416388 39.0 34.0 40.0 11.0 41.0 24 33.90693323427105 39.0 34.0 40.0 11.0 41.0 25 33.71762070401765 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.659685321728464 1101 2 0.6579096055095555 1101 3 0.696991257357741 1101 4 0.6080071498018143 1101 5 0.6562031014147323 1101 6 0.034846806642129025 1101 7 0.4870202571921922 1101 8 0.6085022189434781 1101 9 0.9138883419240287 1101 10 0.8630217175269195 1101 11 0.8139337229761878 1101 12 0.7410610244627094 1101 13 0.6823669243862724 1101 14 1.063016203452655 1101 15 0.9064363396590096 1101 16 0.7241510957591117 1101 17 0.6801893780612289 1101 18 0.7838770995413782 1101 19 0.8626035041316626 1101 20 0.8452478012874991 1101 21 0.8878594326615925 1101 22 0.8241950172184858 1101 23 0.9992703024908636 1101 24 0.8991975041819344 1101 25 0.8882073384078595 1102 1 0.8013625577488206 1102 2 0.7524160835868408 1102 3 0.684045136979492 1102 4 0.3645819567438693 1102 5 0.578313654178551 1102 6 0.3227612189593074 1102 7 0.46012700498867787 1102 8 0.767822783427242 1102 9 0.9779843812259656 1102 10 1.0046925316614903 1102 11 0.8089346862590574 1102 12 0.8322309315059115 1102 13 0.7325672069492484 1102 14 1.0682171282042034 1102 15 0.8237180356790503 1102 16 0.8533650569388058 1102 17 0.9996765904811582 1102 18 1.0379293094728297 1102 19 1.0203446860797385 1102 20 0.8526125001813298 1102 21 1.0148313529659916 1102 22 1.016471094088459 1102 23 1.0005442440838124 1102 24 0.9304708838598756 1102 25 1.1923109234256017 1103 1 -0.0018323304300942311 1103 2 -0.16196091321826955 1103 3 -0.31339895247737815 1103 4 -0.21816564708999664 1103 5 -0.10091957265851192 1103 6 -0.3967275989320669 1103 7 -0.17246085443623826 1103 8 0.06878124988091372 1103 9 3.4859847836798963E-4 1103 10 -0.037479992300170295 1103 11 -0.3284455054342317 1103 12 -0.11217801418099071 1103 13 -0.22142640327439267 1103 14 -0.06085635422390823 1103 15 -0.09513180457654613 1103 16 0.013748443520277931 1103 17 -0.1690135053654913 1103 18 -0.21446685666136034 1103 19 -0.04738694331323501 1103 20 -0.0017005988602036837 1103 21 -0.08484915821611594 1103 22 -0.08896021581569613 1103 23 -0.046101123328327276 1103 24 -0.23694125671519828 1103 25 -0.19171463567982983 1104 1 0.5802161939111592 1104 2 0.8100533688808653 1104 3 0.5949619275403357 1104 4 0.28929726373782927 1104 5 0.6036849413072645 1104 6 0.2038726288223387 1104 7 0.3567743494355824 1104 8 0.278215264368626 1104 9 0.7106786051212026 1104 10 0.7137698890777457 1104 11 0.6165731577739422 1104 12 0.6394763685096763 1104 13 0.3965784523229843 1104 14 0.8275847143238408 1104 15 0.6645244286186625 1104 16 0.6206198255466617 1104 17 0.44848008904187253 1104 18 0.46094436656959203 1104 19 0.5496277341928533 1104 20 0.7292407860131078 1104 21 0.7041624825645769 1104 22 0.6066966342143516 1104 23 0.5926455947939644 1104 24 0.5086898918629146 1104 25 0.9267540322732302 1105 1 0.9943701093026966 1105 2 0.7052866422082005 1105 3 0.7689355749050719 1105 4 0.5579303770491606 1105 5 0.634500522865423 1105 6 0.21642388394784717 1105 7 0.3913715591565605 1105 8 0.5219583886810568 1105 9 0.9457458618468735 1105 10 1.158726884777316 1105 11 0.7408980902671942 1105 12 0.7760275236251886 1105 13 0.5440744519229455 1105 14 0.9959828041328223 1105 15 0.8159333195077494 1105 16 0.9563283964037481 1105 17 0.9041881598489567 1105 18 0.969663988531785 1105 19 1.1058208535047882 1105 20 1.0246390758420887 1105 21 0.9955728736036775 1105 22 1.0675614706980028 1105 23 1.2543430145359338 1105 24 0.9168869615698867 1105 25 1.0809069475647561 1106 1 0.6217771849856355 1106 2 0.8092760436113942 1106 3 0.6841592401287393 1106 4 0.6974692385707826 1106 5 0.6805304029470207 1106 6 -0.5781287856048181 1106 7 -0.3984291573682981 1106 8 -0.2262627388504157 1106 9 -0.24057547964687842 1106 10 -0.01172455076032719 1106 11 -0.2730405572921484 1106 12 -0.15582797719583397 1106 13 -0.3803048086632117 1106 14 -0.15575004156553973 1106 15 -0.23862084280503382 1106 16 -0.15151108212538844 1106 17 -0.22390931738645037 1106 18 -0.22052297967242396 1106 19 -0.1977567949004495 1106 20 -0.16745116150676864 1106 21 -0.004672809831660629 1106 22 -0.15977656482968428 1106 23 0.17529203677806038 1106 24 -0.11193574828730846 1106 25 -0.010157639189486645 1107 1 0.8386903838715902 1107 2 0.731292557716877 1107 3 0.7498217850730526 1107 4 0.5291934218619332 1107 5 0.6169876873277218 1107 6 0.47538783282892894 1107 7 0.7534244080878594 1107 8 0.8571333426405374 1107 9 1.1420109994103527 1107 10 0.7258448880141017 1107 11 0.9144150275962843 1107 12 0.8678783523544666 1107 13 0.8843347901114598 1107 14 1.1143278568701405 1107 15 1.1068047409040318 1107 16 1.1311916700239237 1107 17 1.056791541734107 1107 18 1.2763879965679479 1107 19 1.200120200566566 1107 20 0.8883905407675883 1107 21 1.056531213912848 1107 22 1.0445586524603812 1107 23 1.2102661966331496 1107 24 1.0144719272487777 1107 25 1.219719102260889 1108 1 1.298866612799202 1108 2 1.175794800716357 1108 3 0.9767086906781905 1108 4 0.7439773855961676 1108 5 -0.5644555765906816 1108 6 0.5215855001094667 1108 7 0.933924006709006 1108 8 1.1971036878263561 1108 9 1.5845609356619477 1108 10 1.570244755795187 1108 11 1.3039889993581042 1108 12 1.10492495952262 1108 13 1.1309687798172732 1108 14 1.5796292687792857 1108 15 1.5453420219164542 1108 16 1.3831994142747632 1108 17 1.4831998148606118 1108 18 1.4866115409793395 1108 19 1.5265683282522602 1108 20 1.5716215933246076 1108 21 1.4315981261289323 1108 22 1.709252612889145 1108 23 1.7994117791047017 1108 24 1.7209157105986321 1108 25 1.7378385714963827 1109 1 0.5471793492430841 1109 2 -0.20823546410079175 1109 3 0.5491475305268665 1109 4 0.7636999581056259 1109 5 -0.840047001559153 1109 6 0.28184779519143177 1109 7 0.6348067305425502 1109 8 0.6635248731983623 1109 9 1.325443659495491 1109 10 -0.9430768078870315 1109 11 0.29769391739088746 1109 12 0.795235173898412 1109 13 0.9022272917970966 1109 14 1.3077604163202707 1109 15 1.2098867886251554 1109 16 1.1687832730389474 1109 17 1.2356436988454789 1109 18 1.0389747187851412 1109 19 1.159748656121117 1109 20 1.20807052144567 1109 21 1.205762060555159 1109 22 1.2290306708967123 1109 23 1.0452831536318143 1109 24 1.2205374003842593 1109 25 1.26532406960483 1110 1 0.46111876893859005 1110 2 -0.3838745746263825 1110 3 0.1470099021044824 1110 4 0.48902150544262213 1110 5 0.6301607053775129 1110 6 0.601461067186591 1110 7 0.7357346652662642 1110 8 0.6275247457056139 1110 9 0.8627423006352757 1110 10 0.8543091657857147 1110 11 0.5944154413542151 1110 12 0.7258276964486896 1110 13 0.5721345408223115 1110 14 1.0404251210151898 1110 15 0.7768606419872413 1110 16 0.3512309213289555 1110 17 0.7882491194121855 1110 18 0.9145507204000509 1110 19 1.0353641804881661 1110 20 0.764855154319676 1110 21 0.8144056439513534 1110 22 0.9005179800560015 1110 23 1.237015248868893 1110 24 1.0895721740315096 1110 25 1.0501137596912073 1111 1 0.07555991432971254 1111 2 0.010137476128800671 1111 3 0.2805047550789048 1111 4 0.06261383958145927 1111 5 0.3160397523697682 1111 6 -0.20649718722903287 1111 7 0.1618965767746019 1111 8 0.2906922388102515 1111 9 0.4697944876396889 1111 10 0.5650135890291352 1111 11 0.1758287926397557 1111 12 0.19883114119624423 1111 13 0.11886303543246157 1111 14 0.3808489070221768 1111 15 0.21201624175346012 1111 16 0.16066830713900515 1111 17 0.22054944714047764 1111 18 0.1889645523090948 1111 19 0.1516277597768081 1111 20 -0.12706693778252287 1111 21 0.010533526645723157 1111 22 0.22193955385515807 1111 23 0.4939043253181765 1111 24 0.3569562591973252 1111 25 0.45655294239679733 1112 1 0.45655050702997 1112 2 0.1997823808445638 1112 3 0.46373513298588165 1112 4 0.7237289881602749 1112 5 0.791204279178551 1112 6 0.5565438334428023 1112 7 0.796556218247467 1112 8 0.6621493609032783 1112 9 0.48370356386707414 1112 10 0.8706902686156965 1112 11 0.5966035826914364 1112 12 0.45311085695851716 1112 13 0.41193272212496623 1112 14 0.6136240604459502 1112 15 0.6626228712754241 1112 16 0.7673684847875819 1112 17 0.4898889174247145 1112 18 0.5182553711075428 1112 19 0.7224954115855979 1112 20 0.6975202311451199 1112 21 0.6784366677955944 1112 22 0.7313872282737606 1112 23 0.9108889425662383 1112 24 0.6460338997012016 1112 25 0.7102472253425347 1113 1 -0.0625809805431281 1113 2 -0.04803605848292136 1113 3 -1.1832175351427736 1113 4 -1.1990140673952823 1113 5 -1.2111600300319765 1113 6 -0.5446074823466738 1113 7 -0.1220521516355717 1113 8 -0.3018720206756704 1113 9 0.38826313989046923 1113 10 0.13211205224143185 1113 11 0.14121706953353907 1113 12 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23 -3.891711136843327 1114 24 -1.5863120690955128 1114 25 -1.1500970384840699 1115 1 -0.5023249287270026 1115 2 -2.1734408719774834 1115 3 -2.25565293972328 1115 4 -2.122210171866289 1115 5 -1.1248735968174657 1115 6 -1.2854423883367474 1115 7 -9.567565420198747 1115 8 -11.404225763598713 1115 9 -15.800275104791623 1115 10 -6.931123310401567 1115 11 -4.631883510602059 1115 12 -2.7062101921782684 1115 13 -2.4541498226957508 1115 14 -2.204211368670915 1115 15 -4.15255132776176 1115 16 -4.247950447815338 1115 17 -5.079935949773162 1115 18 -3.0721460440650965 1115 19 -4.061861950632199 1115 20 -3.248925755740668 1115 21 -4.9588241846280425 1115 22 -3.181585879295959 1115 23 -2.324349790003481 1115 24 -5.056004301660021 1115 25 -5.137829218880572 1116 1 -0.9742487718161854 1116 2 -2.904474430052872 1116 3 -3.322983617014117 1116 4 -3.621022614403831 1116 5 -1.704378653513757 1116 6 -1.7126068075828407 1116 7 -1.642806762521765 1116 8 -1.2442809275582611 1116 9 -1.746785218184204 1116 10 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18 0.5136980794408785 2202 19 0.5093183282522631 2202 20 0.5639004394784521 2202 21 0.6268481261289267 2202 22 0.7038872282737572 2202 23 0.8923733175662392 2202 24 0.6148099413678665 2202 25 0.8330597253425367 2203 1 0.00114164117044524 2203 2 -0.17631967108509272 2203 3 -0.07692845344772792 2203 4 -0.3509098695476567 2203 5 0.09787747714199213 2203 6 -0.012587687831519645 2203 7 0.33678382809352314 2203 8 0.42731082257742514 2203 9 0.5759518667321615 2203 10 0.4966089932254931 2203 11 0.3954989416287873 2203 12 0.5349809125497131 2203 13 0.2404310583982152 2203 14 0.7977844964893563 2203 15 0.5336193199872099 2203 16 0.3656177297629952 2203 17 0.366596074790543 2203 18 0.5536375438411341 2203 19 0.5489775649424473 2203 20 0.5210929507035971 2203 21 0.6514416155452238 2203 22 0.5200137516862071 2203 23 0.7803928813892043 2203 24 0.660395653362734 2203 25 0.651741171782561 2204 1 0.44165823522946823 2204 2 0.5434106679106421 2204 3 0.3850464959093429 2204 4 -0.008473150066407698 2204 5 0.3443111447682625 2204 6 0.24114886271925684 2204 7 0.44522407427005106 2204 8 0.40980168994970256 2204 9 0.7629375402367984 2204 10 0.47321666970311327 2204 11 0.5047568789064059 2204 12 0.46418101588783856 2204 13 0.3596026954624847 2204 14 0.6486782022799105 2204 15 0.5975211552047455 2204 16 0.629457111408243 2204 17 0.542357811193007 2204 18 0.6735749614985949 2204 19 0.7063525189674422 2204 20 0.7216349125022958 2204 21 0.6790109241213997 2204 22 0.6958241793402564 2204 23 0.9865608959853134 2204 24 0.8576322437518087 2204 25 0.7496822159322463 2205 1 0.5634819974145842 2205 2 0.5357948007163564 2205 3 0.4062471522166504 2205 4 0.4430543086730907 2205 5 0.3050828849477796 2205 6 0.17697011549407904 2205 7 0.13823169901669985 2205 8 0.3644883032109689 2205 9 0.6510224741234865 2205 10 0.5573216788721069 2205 11 0.479988999358099 2205 12 0.5541557287533934 2205 13 0.40112262597111936 2205 14 0.729167730317748 2205 15 0.7890343296087607 2205 16 0.6927378758132221 2205 17 0.90966135332215 2205 18 0.7309192332870325 2205 19 0.6994914051753369 2205 20 0.7636215933246078 2205 21 0.7663673568981579 2205 22 0.6175603051968395 2205 23 1.0049502406431614 2205 24 0.8070695567524808 2205 25 0.9673770330348432 2206 1 0.8626133525459423 2206 2 0.832846019767576 2206 3 1.0745593805288784 2206 4 0.8186627002814859 2206 5 1.0125888384537376 2206 6 0.869747662271628 2206 7 1.1152659080509082 2206 8 1.1974975642202281 2206 9 1.42428121538223 2206 10 1.365917029467461 2206 11 1.0781216774907776 2206 12 1.0652371878348532 2206 13 0.7371880200365126 2206 14 1.0782582614082799 2206 15 0.909265287839716 2206 16 0.8457974108727626 2206 17 1.021919527580323 2206 18 1.1230730794408785 2206 19 0.8674233651072996 2206 20 0.9670530807560951 2206 21 1.221224661755464 2206 22 1.035364501001034 2206 23 1.3821583298512508 2206 24 1.3484662681491955 2206 25 1.3141515558093673 2207 1 -0.4252103102777234 2207 2 0.341999928921485 2207 3 0.5500933060628057 2207 4 0.4994902061089874 2207 5 0.7565187823836794 2207 6 0.7159188334427995 2207 7 0.7639240067090043 2207 8 0.7358472775699454 2207 9 1.161868627969639 2207 10 1.034552448102879 2207 11 0.7717838711529694 2207 12 0.6638480364456996 2207 13 0.5604303182788115 2207 14 -4.8694989363489185 2207 15 -1.6008887473143147 2207 16 -0.30977494469959765 2207 17 -0.11644121078041536 2207 18 0.3974320537998537 2207 19 0.583132430816363 2207 20 0.8014164651194804 2207 21 0.7522647927956001 2207 22 0.7978936385301694 2207 23 1.0423092150021347 2207 24 0.8063003259832513 2207 25 0.6227872894450996 2208 1 0.46996869892714344 2208 2 0.7305786482578718 2208 3 0.7799628652468549 2208 4 0.5678563809243542 2208 5 0.7142126319296054 2208 6 0.7600509240421189 2208 7 0.6243500208632184 2208 8 0.6437596848659979 2208 9 1.0348426323176696 2208 10 1.0155182652082004 2208 11 0.7631880372414415 2208 12 0.7727453027392386 2208 13 0.5234616795813736 2208 14 1.0761584329404386 2208 15 0.865796807059148 2208 16 0.8215963799068433 2208 17 0.6925316063403244 2208 18 0.8604452983224178 2208 19 0.33988883336350995 2208 20 0.586448921138107 2208 21 0.811725606585938 2208 22 0.7161850334451358 2208 23 1.0595906957983487 2208 24 0.9000673827388823 2208 25 0.8799565232980342 2209 1 -0.5073888093637358 2209 2 -0.36524526647399824 2209 3 0.2260208788111875 2209 4 0.4125787392842284 2209 5 0.350311085135516 2209 6 0.19490085014157899 2209 7 0.5502216204632688 2209 8 0.5739815100683359 2209 9 0.5823357491593057 2209 10 0.37369083059386554 2209 11 0.40320030314743605 2209 12 0.5509954105872907 2209 13 0.48230794290210355 2209 14 0.5954237453367668 2209 15 0.6059797374443434 2209 16 0.5032061333103854 2209 17 0.5773066277651111 2209 18 0.6065348649257842 2209 19 0.6051348022406984 2209 20 0.5027182782710042 2209 21 0.5862082738489036 2209 22 0.5726886091343886 2209 23 0.6576061370909656 2209 24 0.6172212291006858 2209 25 0.6750784311871101 2210 1 -0.29259032784242933 2210 2 -0.367387717235804 2210 3 0.4154985858065672 2210 4 0.6445983495885272 2210 5 0.9510470322846629 2210 6 0.9659803960127604 2210 7 1.1919164642179183 2210 8 1.0766184886735957 2210 9 1.5100617880667642 2210 10 1.354617540834191 2210 11 0.8748043116482265 2210 12 0.9256525516081737 2210 13 0.8355155587602923 2210 14 1.3426809813380487 2210 15 1.308153201334747 2210 16 1.3601644398469048 2210 17 1.3986119138428919 2210 18 1.3492315750755353 2210 19 1.2012869313415848 2210 20 1.462444344784032 2210 21 1.5259272060483369 2210 22 1.5646855828741622 2210 23 1.6781338951350762 2210 24 1.6121819192053408 2210 25 1.5394268341403858 2211 1 -1.2737920250319874 2211 2 -0.17935591931644268 2211 3 0.42678320510447065 2211 4 0.5510229449668458 2211 5 0.6675874716335812 2211 6 0.5162130817217232 2211 7 0.5662019752428051 2211 8 0.5229947348206636 2211 9 0.7027016623569722 2211 10 0.5078522994840071 2211 11 0.36068187077715663 2211 12 0.6396462222705352 2211 13 0.6697894634640704 2211 14 0.7536838990257877 2211 15 0.46452533560476184 2211 16 0.461240105096266 2211 17 0.6405558339248216 2211 18 0.5293022599871833 2211 19 0.5094490744214824 2211 20 0.5499593168935135 2211 21 0.6364642154027464 2211 22 0.5995792669146667 2211 23 0.9388273482124774 2211 24 0.9485670199821215 2211 25 1.1026054448628528 2212 1 1.3140414173760675 2212 2 0.674715838441518 2212 3 0.9039677821022281 2212 4 0.9153289206590429 2212 5 1.1357510621314262 2212 6 0.9310759272081413 2212 7 1.1437982561708822 2212 8 1.0836504194452488 2212 9 1.552343874372724 2212 10 0.985894920063906 2212 11 1.1974853174729745 2212 12 1.404218490733676 2212 13 1.1528258093856465 2212 14 1.202345027247624 2212 15 1.1539062000064035 2212 16 0.635961621140062 2212 17 1.3394762394669328 2212 18 1.278816820236166 2212 19 1.2377848230976198 2212 20 1.1998549681971582 2212 21 1.367851439825543 2212 22 1.4905459175226525 2212 23 1.556176704900551 2212 24 1.4705067381278099 2212 25 1.065422575121623 2213 1 0.020752352790204043 2213 2 -0.06265594152385745 2213 3 -0.5896502836807862 2213 4 -1.1734292720736441 2213 5 -2.4618910241840233 2213 6 -1.1403677162648016 2213 7 -0.6988065376004862 2213 8 -0.7207316697984751 2213 9 -1.2257719478288323 2213 10 -0.9775370705655888 2213 11 -1.1168238661389722 2213 12 -1.1757268623397295 2213 13 -1.1009282062376045 2213 14 -8.760861959290889 2213 15 -3.951743448169019 2213 16 -2.634381332463878 2213 17 -1.9544641527507238 2213 18 -5.826488324067892 2213 19 -3.676741613268206 2213 20 -5.75682689970283 2213 21 -2.7398404703622994 2213 22 -2.5246434734806265 2213 23 -5.36916177015306 2213 24 -2.9951279241292106 2213 25 -2.4497948068212096 2214 1 0.36970932232847886 2214 2 0.33114132600844925 2214 3 -1.7912891481818107 2214 4 -1.8497066827498827 2214 5 -0.18200241254664462 2214 6 -0.4985163436130975 2214 7 -0.5905603587937982 2214 8 -0.5244761055146334 2214 9 -5.317517810876851 2214 10 -1.4476856825110218 2214 11 -1.0319688584118722 2214 12 -1.2365200327107786 2214 13 -1.434296837796019 2214 14 -2.4593663414050866 2214 15 -1.9390007889162604 2214 16 -1.1591581193242106 2214 17 -1.6149407943107263 2214 18 -0.8617820566434062 2214 19 -4.303760029956692 2214 20 -2.1083773261053906 2214 21 -3.888809248761323 2214 22 -5.3982495144830445 2214 23 -5.516869439237976 2214 24 -5.392933144672522 2214 25 -3.261705968248336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31176.0 3 21.0 4 525.0 5 3663.0 6 4525.0 7 3765.0 8 2784.0 9 2274.0 10 2069.0 11 1807.0 12 1753.0 13 1779.0 14 1640.0 15 1819.0 16 1876.0 17 1986.0 18 2154.0 19 2501.0 20 3230.0 21 4128.0 22 5513.0 23 6744.0 24 8097.0 25 9383.0 26 10730.0 27 12038.0 28 14150.0 29 16824.0 30 20893.0 31 26158.0 32 33231.0 33 43079.0 34 56260.0 35 73755.0 36 99564.0 37 146372.0 38 230064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.692544120563156 19.96094947091378 9.593856470715483 29.752649937807586 2 13.58421728261604 21.65900952638335 39.27583721820598 25.480935972794633 3 18.318792732251758 24.86713028438623 29.170870454279306 27.643206529082704 4 12.081169459996307 17.810910876194168 37.671439574447 32.436480089362526 5 12.959954242427314 38.69735036181584 35.371481908081776 12.971213487675067 6 31.486053593523128 38.472985723083184 17.569251803682505 12.471708879711185 7 27.877008621301957 32.24391734283063 21.67941203329466 18.19966200257275 8 24.918019107073587 38.3296195725717 19.74713348789795 17.005227832456765 9 25.85572855759006 16.14328131995277 19.992128639235688 38.00886148322149 10 14.515729529994303 30.32463336042927 33.88947559226392 21.270161517312502 11 33.89244556817248 23.46312617286925 24.11098340337246 18.533444855585813 12 22.815387524991447 25.63548064626524 31.51477422143771 20.03435760730561 13 28.407686332781733 22.67850911260455 25.754167933087928 23.159636621525785 14 20.442288952351984 23.047805735336365 27.68485503940157 28.825050272910087 15 22.498060948688593 31.4817504032889 23.344376638625004 22.675812009397504 16 21.698466858867484 29.26668370281896 27.590348832104215 21.444500606209342 17 21.080520638437275 29.479654258612577 27.859132224241073 21.580692878709076 18 21.042511682466564 28.703237768449064 29.45467563213655 20.799574916947822 19 22.791040105188028 27.778353149899697 27.68289141001943 21.747715334892842 20 22.79401252475175 28.1855320317768 28.300693105307655 20.719762338163793 21 22.33825794076062 27.438063418016768 27.5276699597446 22.696008681478013 22 22.26695034502944 28.69057670010019 27.44126619612081 21.601206758749562 23 21.853365303434536 28.36761113550145 27.74351873740614 22.035504823657874 24 22.329227121933428 27.711579831346267 28.458262648185528 21.500930398534777 25 22.095529921909563 28.053345100745332 28.04298857743021 21.808136399914897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 283.0 1 283.0 2 287.5 3 292.0 4 292.0 5 292.0 6 494.0 7 696.0 8 696.0 9 696.0 10 1253.0 11 1810.0 12 1810.0 13 1810.0 14 3056.5 15 4303.0 16 4303.0 17 4303.0 18 7619.5 19 10936.0 20 10936.0 21 10936.0 22 16977.5 23 23019.0 24 23019.0 25 23019.0 26 32364.0 27 41709.0 28 41709.0 29 41709.0 30 54956.0 31 68203.0 32 68203.0 33 68203.0 34 78235.0 35 88267.0 36 88267.0 37 88267.0 38 95513.0 39 102759.0 40 102759.0 41 102759.0 42 107990.0 43 113221.0 44 113221.0 45 113221.0 46 116207.0 47 119193.0 48 119193.0 49 119193.0 50 113396.0 51 107599.0 52 107599.0 53 107599.0 54 94806.5 55 82014.0 56 82014.0 57 82014.0 58 69694.0 59 57374.0 60 57374.0 61 57374.0 62 46263.0 63 35152.0 64 35152.0 65 35152.0 66 26879.0 67 18606.0 68 18606.0 69 18606.0 70 13377.5 71 8149.0 72 8149.0 73 8149.0 74 5663.0 75 3177.0 76 3177.0 77 3177.0 78 2136.5 79 1096.0 80 1096.0 81 1096.0 82 732.5 83 369.0 84 369.0 85 369.0 86 226.5 87 84.0 88 84.0 89 84.0 90 49.5 91 15.0 92 15.0 93 15.0 94 9.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08577893350444092 2 0.030394110296849144 3 0.027242128488287012 4 0.02949354406583139 5 0.019249603188004456 6 0.37542354755552554 7 0.15095741447435074 8 0.1731338579131629 9 0.1768486936161111 10 0.01440905969628404 11 0.001913703240912724 12 0.0029268402508076954 13 0.0 14 0.07598527574212285 15 0.0010131370098949715 16 0.0025891279141760383 17 0.004052548039579886 18 0.003714835702948229 19 0.001801132462035505 20 0.0010131370098949715 21 2.251415577544381E-4 22 0.0 23 0.0 24 1.1257077887721906E-4 25 1.1257077887721906E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 888330.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.49671328636644 #Duplication Level Percentage of deduplicated Percentage of total 1 91.81545493402209 77.58104170817364 2 6.312953329413548 10.668476149313381 3 1.0291658862608979 2.6088340444648894 4 0.3219349674875771 1.0880978657861402 5 0.14775295574394148 0.6242319569354506 6 0.08029357593260385 0.4070725958588592 7 0.055695570153982396 0.3294264835835231 8 0.038176349131963584 0.2580620821539001 9 0.029364806066752257 0.22331066390389076 >10 0.1442009052564418 2.2178408944331074 >50 0.013107724087523497 0.7547781681932607 >100 0.010027244414318359 1.7052703568368306 >500 0.0012032691610295084 0.7960940252647042 >1k 6.684828672386157E-4 0.7374630050983382 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1744 0.19632343836187 No Hit CTGTAGGACGTGGAATATGGCAAGA 1364 0.15354654238852677 No Hit GTCCTACAGTGGACATTTCTAAATT 1320 0.14859342811792914 No Hit GGTATCAACGCAGAGTACTTTTTTT 1077 0.1212387288507649 No Hit CTTTAGGACGTGAAATATGGCGAGG 1023 0.11515990679139508 No Hit GTCCTAAAGTGTGTATTTCTCATTT 987 0.1111073587518152 No Hit CCCATGTACTCTGCGTTGATACCAC 923 0.10390282890367318 No Hit GTACATGGGAAGCAGTGGTATCAAC 904 0.10176398410500602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1257077887721906E-4 0.0 10 0.0 0.0 0.0 1.1257077887721906E-4 0.0 11 0.0 0.0 0.0 1.1257077887721906E-4 0.0 12 0.0 0.0 0.0 1.1257077887721906E-4 0.0 13 0.0 0.0 0.0 1.1257077887721906E-4 1.1257077887721906E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 420 0.0 14.522159 4 TGTAGGA 935 0.0 13.6628475 2 GGCGTGC 50 0.0014985937 13.300079 8 CTGAGCG 50 0.0014985937 13.300079 9 GTAGGAC 890 0.0 13.27949 3 CTGTAGG 1005 0.0 12.612777 1 CCCCTAT 70 1.0575283E-4 12.253767 1 AGGACCT 700 0.0 11.980782 5 TTAGCCT 65 7.8069686E-4 11.729437 4 TGGCGAG 855 0.0 11.639811 18 GGACCTG 680 0.0 11.631752 6 GTCCTAG 125 1.7416824E-8 11.436849 1 GGCGAGA 565 0.0 11.238921 19 TGGAATA 1155 0.0 11.16794 11 GGCGAGG 315 0.0 11.132416 19 CGCCTTT 60 0.0059738895 11.057819 16 GTCCTAA 345 0.0 11.050096 1 ATGGCGA 980 0.0 10.928865 17 GTTCTAA 105 3.3288525E-6 10.892237 1 TTGTACC 70 0.0014513955 10.89162 4 >>END_MODULE