FastQCFastQC Report
Thu 26 May 2016
SRR1926632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851570
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1850.015.014784
GTTCTAA801.6188631E-514.9593761
TGTAGGA4050.014.789142
GTCCTAC5150.014.4591161
CTGTAGG4050.013.7897131
TGCCGGT400.006624629313.76864210
GACCATC450.007432430513.5310547
CAGGAGT652.9797322E-413.1490384
TATACCT652.9797322E-413.1490384
CCTAGAC500.00940636113.0570323
GTAGGAC4000.013.0570323
ACCGTGC450.0098366412.9691538
TCCTACA5250.012.7052152
GTCATGT750.002518808512.4107421
CCCTTAA750.002518808512.4107421
TAGTACT705.567609E-412.2098214
CCTTATA906.0022227E-412.1002052
TAGGACG3900.012.0532844
CTTTAGG2101.4551915E-1112.0308211
GTATCAA5250.011.904181
Produced by FastQC (version 0.11.5)