FastQCFastQC Report
Thu 26 May 2016
SRR1926632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851570
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17990.21125685498549737No Hit
CTGTAGGACGTGGAATATGGCAAGA12300.14443909484833894No Hit
GTCCTACAGTGGACATTTCTAAATT12190.14314736310579282No Hit
GGTATCAACGCAGAGTACTTTTTTT10890.12788144251206596No Hit
CTTTAGGACGTGAAATATGGCGAGG9770.11472926476977817No Hit
TATCAACGCAGAGTACTTTTTTTTT9350.10979719811642025No Hit
GTCCTAAAGTGTGTATTTCTCATTT9040.10615686320560846No Hit
GTCCTACAGTGTGCATTTCTCATTT8820.10357339972051623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3950.014.001084
GTATTAC655.2725813E-513.19949151
GTTGTAA655.2725813E-513.19949151
AGGACCT6050.012.6085535
GTAGGAC7800.012.1045383
TGTAGGA8100.012.0108612
TGGCGAG6750.011.65043518
GGCGAGG2550.011.51832619
TAGAGGT1001.8421142E-611.4422624
GGACCCT1001.8421142E-611.4422626
TATGGCG7650.011.39447316
GTCCTAA2850.011.372661
GTCCTAC6850.011.2725571
CTGTAGG8350.011.188391
AGGACGT6250.011.13713555
CTAGGAC1201.2055716E-711.1263933
GGACCTA600.005712376411.1244216
ATGGCGA7900.011.03388817
GGACCTG6400.011.0250966
TAGGACG6550.010.9181894
Produced by FastQC (version 0.11.5)