FastQCFastQC Report
Thu 26 May 2016
SRR1926631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902449
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGA250.0079230118.12157613
TGTAGGA4000.016.3142412
TAGGACC2100.015.7922624
ATAGCCC905.999027E-915.7295433
GTAGGAC4350.014.5195783
GTCCTAG658.698273E-414.2916861
GATTAAG658.698273E-414.2916861
TATAGCC1057.5216667E-714.2425922
GTATTGG751.5939873E-414.1555751
GTACTAA751.5939873E-414.1555751
GTCCTAA1703.6379788E-1214.0514911
CTGTAGG4350.014.0335451
CTCTAGG955.370548E-613.9693171
TAGCCCT1159.0039975E-1013.9538924
CTAGGAC1251.6189006E-1013.9387933
GTATAGC1059.878459E-713.9027971
AGGATAG450.006423405413.8327455
TAGGACA1850.013.3001974
GTATAAC600.00844761513.2708511
GTAATAG500.00935695413.0676193
Produced by FastQC (version 0.11.5)