##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926631_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 902449 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.406599154079622 27.0 10.0 31.0 2.0 31.0 2 16.64124842511876 22.0 2.0 30.0 2.0 31.0 3 20.06799054572613 25.0 15.0 31.0 2.0 31.0 4 22.170144794885918 28.0 7.0 35.0 2.0 37.0 5 22.76905620151388 29.0 7.0 35.0 2.0 37.0 6 23.721675130672203 31.0 2.0 35.0 2.0 37.0 7 23.608571786328092 31.0 7.0 35.0 2.0 37.0 8 23.68202746083158 31.0 10.0 35.0 2.0 37.0 9 26.457821993264993 32.0 17.0 37.0 2.0 39.0 10 27.181973718182412 32.0 24.0 37.0 2.0 39.0 11 24.275289794769566 30.0 8.0 37.0 2.0 39.0 12 25.427875702671287 31.0 15.0 37.0 2.0 39.0 13 22.078138487604285 28.0 2.0 37.0 2.0 39.0 14 25.42551878277886 31.0 17.0 38.0 2.0 40.0 15 26.519436555417535 31.0 17.0 38.0 2.0 40.0 16 26.88413528077487 32.0 17.0 39.0 2.0 40.0 17 26.189169692691774 31.0 16.0 38.0 2.0 40.0 18 26.25591695486393 31.0 17.0 38.0 2.0 40.0 19 26.65356158630571 31.0 18.0 39.0 2.0 40.0 20 26.920490797817937 31.0 18.0 39.0 2.0 40.0 21 24.753442022762506 30.0 8.0 38.0 2.0 40.0 22 25.702926148735276 31.0 8.0 38.0 2.0 40.0 23 25.694694104597602 31.0 2.0 38.0 2.0 40.0 24 24.75549532438952 31.0 2.0 39.0 2.0 40.0 25 24.551363013311555 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.815996244154192 1101 2 10.600801931299765 1101 3 7.107462403564579 1101 4 8.661944006485996 1101 5 8.14183661694732 1101 6 7.94578910946975 1101 7 7.735186255221841 1101 8 6.177802695209522 1101 9 6.133281017151919 1101 10 5.642304930134422 1101 11 8.594496886376977 1101 12 6.169978104553035 1101 13 10.605516889199944 1101 14 8.03464747520838 1101 15 7.298302098338734 1101 16 6.7661770156921754 1101 17 2.4331084642277645 1101 18 5.25303322493075 1101 19 5.954970657783758 1101 20 4.046355588371238 1101 21 1.24199679776844 1101 22 5.030701743429713 1101 23 6.026466610700666 1101 24 7.759087266198204 1101 25 8.151164110099085 1102 1 5.195563825141626 1102 2 10.18349925966512 1102 3 6.209118505724703 1102 4 7.661356834036599 1102 5 7.281369676923635 1102 6 6.397228962716046 1102 7 6.391453927043674 1102 8 6.332124225325757 1102 9 5.410215582890604 1102 10 4.83398205622084 1102 11 7.416540679983889 1102 12 4.811209038689231 1102 13 9.324186805675645 1102 14 6.6354127697699425 1102 15 4.576213183090577 1102 16 4.967552733155824 1102 17 5.914383377186343 1102 18 5.876756174112746 1102 19 4.461185030898353 1102 20 4.711368977085311 1102 21 3.9312875998499486 1102 22 5.0427829150949 1102 23 5.3141629834330075 1102 24 6.8059274705657415 1102 25 6.404085293034829 1103 1 4.585275060278729 1103 2 8.961322199081119 1103 3 5.78747529614774 1103 4 7.2630594952029846 1103 5 6.774791580992229 1103 6 6.3984260831560675 1103 7 6.536170015371749 1103 8 6.232633810377926 1103 9 5.0350275217404 1103 10 4.23409731950645 1103 11 5.2357835821920915 1103 12 3.9839361275835117 1103 13 7.977021878069078 1103 14 6.394732918601132 1103 15 5.3751070599813 1103 16 4.9891898762440725 1103 17 2.7337354761914376 1103 18 1.6898375459480626 1103 19 3.594006757016608 1103 20 4.104364204780854 1103 21 6.582203639646114 1103 22 2.126538880475472 1103 23 4.393211353569708 1103 24 5.776351315910723 1103 25 1.663787630655147 1104 1 5.5942598294134065 1104 2 10.106111113061162 1104 3 6.755182578455273 1104 4 7.183493038341087 1104 5 7.296739802456376 1104 6 6.9982563174647545 1104 7 7.021882070337394 1104 8 6.902974462854743 1104 9 5.710420305096953 1104 10 4.535496685287452 1104 11 7.8281008838785375 1104 12 4.8526764181070625 1104 13 9.196397588762714 1104 14 6.754197756282711 1104 15 5.943523834753478 1104 16 5.57941873961472 1104 17 5.791634385526951 1104 18 3.559716547853924 1104 19 4.35534542355515 1104 20 3.6234948763162755 1104 21 2.4756007952700045 1104 22 4.6765480894634415 1104 23 4.977747060419592 1104 24 6.060773712169471 1104 25 5.387891155695588 1105 1 3.7872280543910257 1105 2 9.858523944649386 1105 3 6.546761192785848 1105 4 5.918072818324585 1105 5 2.4579162917927313 1105 6 5.273916326163423 1105 7 6.212196773305788 1105 8 3.281583118692513 1105 9 4.029218800281395 1105 10 3.787884835661732 1105 11 7.230867589659422 1105 12 4.266193638792483 1105 13 5.745585693428165 1105 14 3.9984278909177604 1105 15 4.604737443080879 1105 16 4.891446031207135 1105 17 5.677316444072346 1105 18 3.194243350680562 1105 19 2.854702351610772 1105 20 2.3193690713103017 1105 21 3.309546753905842 1105 22 4.733373960972653 1105 23 2.0011090926279955 1105 24 5.0596686934098045 1105 25 5.97508755247156 1106 1 6.15956643118027 1106 2 10.956600821902116 1106 3 7.558851267947443 1106 4 9.065920570097958 1106 5 8.736990383445267 1106 6 7.637104918445072 1106 7 7.498897573438288 1106 8 7.243276526105387 1106 9 6.191015256541693 1106 10 5.52391454873046 1106 11 7.972695069951037 1106 12 5.5931893508197845 1106 13 4.501653680666983 1106 14 5.715505288102317 1106 15 5.738191950608563 1106 16 5.809862047763701 1106 17 6.093127527956373 1106 18 6.245061120239434 1106 19 6.189857594816598 1106 20 5.623269028770427 1106 21 5.862375304139814 1106 22 6.205889645843186 1106 23 6.580138534988414 1106 24 7.290550456381048 1106 25 6.45746670738507 1107 1 4.062813521817191 1107 2 -13.87265215989324 1107 3 -5.5375759859035405 1107 4 1.8031107772542647 1107 5 4.435227478428125 1107 6 4.953297878027865 1107 7 4.603016169217902 1107 8 5.358787656531774 1107 9 3.684104444817322 1107 10 3.0846357810449128 1107 11 5.9523989668074755 1107 12 3.3302694609168455 1107 13 4.797739826787026 1107 14 3.9122970211652373 1107 15 3.818773726647965 1107 16 3.5410873121415065 1107 17 4.0778893223452854 1107 18 -0.5887521976416821 1107 19 1.8352631672730197 1107 20 2.018620615037264 1107 21 -1.3166425142000406 1107 22 1.8484106753472673 1107 23 -1.0566091592508045 1107 24 2.348710290269697 1107 25 3.166659425526941 1108 1 4.87408456199535 1108 2 10.18464578944331 1108 3 0.47118674955159534 1108 4 5.540828509461747 1108 5 7.099273502367996 1108 6 6.941780758235712 1108 7 6.82582622767573 1108 8 6.658420207334409 1108 9 5.173289561269023 1108 10 4.377525453495501 1108 11 6.5689560791764094 1108 12 2.3707467737126144 1108 13 3.0858307611843543 1108 14 4.427321610568594 1108 15 4.5870205485835704 1108 16 4.4633539911802025 1108 17 -0.24348916888603966 1108 18 3.1963289937845047 1108 19 4.181601480347158 1108 20 4.291506575879801 1108 21 4.2267684536017605 1108 22 1.144299698266444 1108 23 3.9914119969151045 1108 24 4.836787120556419 1108 25 5.302205867022721 1109 1 3.7047609060324547 1109 2 -13.87265215989324 1109 3 -5.560943396126609 1109 4 1.9839274222477634 1109 5 1.1689039813790103 1109 6 2.4219564756071357 1109 7 3.8016121480115395 1109 8 3.754110253310806 1109 9 3.3828868795477405 1109 10 2.4797935283691075 1109 11 0.9239500321270739 1109 12 1.5734288087211858 1109 13 2.7944304339691826 1109 14 3.531494780493034 1109 15 3.4529595824046915 1109 16 3.2796836910551797 1109 17 3.6654599935466443 1109 18 3.932156074840062 1109 19 2.2575566553194335 1109 20 1.363525286438687 1109 21 0.49342450590599185 1109 22 2.8287903892614388 1109 23 3.3189699144713103 1109 24 4.320946361090943 1109 25 4.766591805240857 1110 1 3.291859196454041 1110 2 9.467386775862762 1110 3 6.410167437447928 1110 4 7.495175670180938 1110 5 8.031907755970181 1110 6 3.867265830905616 1110 7 6.449943539057667 1110 8 7.110475371801577 1110 9 5.554617697949652 1110 10 5.211760425381755 1110 11 2.9575876055535453 1110 12 5.513142520368749 1110 13 10.03322248040855 1110 14 7.485684931113823 1110 15 6.559550644733125 1110 16 6.213241857142254 1110 17 6.7572990962183965 1110 18 6.86091984349045 1110 19 5.730643389905673 1110 20 5.837837307494709 1110 21 3.887987845706114 1110 22 5.917176411881982 1110 23 6.207190371523417 1110 24 7.425963622271894 1110 25 7.860151962840373 1111 1 5.530247329388931 1111 2 10.010106460796417 1111 3 7.588996476117195 1111 4 8.698559053116334 1111 5 8.834664179657928 1111 6 7.960020810268038 1111 7 7.867753087472064 1111 8 7.925279096126662 1111 9 6.51022646073244 1111 10 5.688106241618819 1111 11 7.802868221690524 1111 12 6.927584146231531 1111 13 8.601698351021412 1111 14 6.832268084584577 1111 15 6.0967713152662455 1111 16 5.8637899887752205 1111 17 7.148818668860841 1111 18 6.969318696980935 1111 19 5.802701797608201 1111 20 5.629595295529189 1111 21 8.175819506537845 1111 22 7.516473371272031 1111 23 7.482735909473572 1111 24 7.403579834518553 1111 25 7.939223688849825 1112 1 -2.328400697445403 1112 2 7.673496789820318 1112 3 5.943384891538557 1112 4 -0.3113182488437616 1112 5 4.126114027906652 1112 6 3.223636222051759 1112 7 5.764607316453862 1112 8 5.968461063964568 1112 9 4.5566673395928206 1112 10 3.190532466154835 1112 11 -0.6246238016398635 1112 12 3.6963359545759538 1112 13 7.88148579946489 1112 14 5.4713275259882295 1112 15 3.533051942385125 1112 16 3.85304108974519 1112 17 4.9211981408342425 1112 18 4.73958741945669 1112 19 4.507902446517008 1112 20 4.1827888567255584 1112 21 -1.9233525959705915 1112 22 2.033902718631797 1112 23 3.4508763228037402 1112 24 5.157450240326007 1112 25 5.2331342823060645 1113 1 -18.722417247413578 1113 2 -13.87265215989324 1113 3 -7.63563933431983 1113 4 -6.245027631050233 1113 5 -1.4667730539115063 1113 6 -0.9571945361323699 1113 7 -20.83882998462825 1113 8 -9.778705023798278 1113 9 -5.71521203109431 1113 10 -4.842552933354877 1113 11 -5.9334205700570415 1113 12 -4.060525322154756 1113 13 -2.7089154833008102 1113 14 -3.0052648633170627 1113 15 -3.4459758075548557 1113 16 -3.642120566485058 1113 17 -3.347858881008456 1113 18 -4.029071601421293 1113 19 -3.662738429745879 1113 20 -4.450564372985092 1113 21 -1.7797141138806367 1113 22 -2.7990910547565413 1113 23 -3.126136973996612 1113 24 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4.879346076169721 2204 8 4.7191734898245095 2204 9 3.174553511899699 2204 10 2.7486135664007847 2204 11 4.397088205371318 2204 12 4.570062124238323 2204 13 2.676448649313457 2204 14 3.9012832344453052 2204 15 4.0266412182444 2204 16 3.959890254607526 2204 17 -1.5501857007949695 2204 18 2.36327781161442 2204 19 3.022406344551726 2204 20 3.473406604222241 2204 21 5.050086184586924 2204 22 4.4442257481781695 2204 23 3.4316270174920227 2204 24 5.013810110019293 2204 25 4.748906417293885 2205 1 6.038519289863071 2205 2 10.28199971934274 2205 3 7.201626440751749 2205 4 8.030723839484704 2205 5 8.065915567911038 2205 6 7.564253847505089 2205 7 7.525926638908409 2205 8 7.3892478679162 2205 9 6.2483954538698825 2205 10 5.604189503970169 2205 11 7.713232750399115 2205 12 6.896575352191313 2205 13 10.073784757826413 2205 14 7.659158957749909 2205 15 6.456005443790961 2205 16 6.009631603340143 2205 17 6.892517882940574 2205 18 6.678124905538567 2205 19 5.221464219455587 2205 20 5.4830077423892725 2205 21 -0.6741912131905146 2205 22 3.9623379704797372 2205 23 5.390762185362682 2205 24 7.277751856220689 2205 25 7.7975996596612625 2206 1 4.414898023522383 2206 2 9.028209909072276 2206 3 6.438324734066143 2206 4 1.880825062968551 2206 5 6.004379808856317 2206 6 6.400829139869469 2206 7 6.509076419312635 2206 8 6.501779320040679 2206 9 4.978083603589582 2206 10 4.006198494193832 2206 11 4.037630872832487 2206 12 4.9854771153313955 2206 13 8.347007352364898 2206 14 0.1798945960041891 2206 15 2.46570324540507 2206 16 3.5532324428728437 2206 17 4.873191709613192 2206 18 -0.6915411328444101 2206 19 1.4466561191487273 2206 20 2.9079987885878538 2206 21 3.68141735696328 2206 22 -1.8819144478054426 2206 23 -2.6218543278752833 2206 24 1.2824647427138025 2206 25 2.879718538827433 2207 1 1.586801702650444 2207 2 -13.87265215989324 2207 3 -6.087925242280967 2207 4 -4.447782948738052 2207 5 1.3565547708540073 2207 6 2.1798141786286713 2207 7 3.1176360845138724 2207 8 3.2843722128205535 2207 9 0.8509390749758943 2207 10 0.6165975657390916 2207 11 -1.082014113070393 2207 12 -1.8242724478785846 2207 13 0.8205689413344501 2207 14 -4.937611380292459 2207 15 -1.083497129256692 2207 16 -0.05328361270829163 2207 17 1.0452246912017813 2207 18 0.9569958571204573 2207 19 0.4183846092113619 2207 20 0.19407496350569886 2207 21 -3.623619762304049 2207 22 -3.848787788161058 2207 23 -1.089492444979161 2207 24 0.7317356029851503 2207 25 1.1893892590736783 2208 1 4.877463846522215 2208 2 7.0667057473600305 2208 3 6.471830764302258 2208 4 8.168238006742971 2208 5 3.935647857098207 2208 6 6.526975734060059 2208 7 6.980432797773652 2208 8 6.734856439116609 2208 9 5.1103453667966505 2208 10 4.689738497591147 2208 11 6.547079658291974 2208 12 6.072681973493449 2208 13 8.438883520192313 2208 14 3.0038711545229617 2208 15 4.490015288890717 2208 16 4.957704160216142 2208 17 5.725005027092383 2208 18 0.49921816730847013 2208 19 3.302907180810017 2208 20 4.3022360911188535 2208 21 6.924495325368241 2208 22 5.842308416132045 2208 23 1.8854923682347895 2208 24 3.114000329600163 2208 25 4.459491767976406 2209 1 2.9112088504534235 2209 2 4.609154615514539 2209 3 5.191875611432625 2209 4 0.46273938453155594 2209 5 4.917915635941878 2209 6 5.409078208111833 2209 7 5.961671897429465 2209 8 4.512346193352542 2209 9 4.044259256128971 2209 10 3.291130906998003 2209 11 -0.49307463612660385 2209 12 3.0747952788880824 2209 13 1.897692341023106 2209 14 3.3683398741717028 2209 15 3.867092228723056 2209 16 3.3406070496185833 2209 17 1.1241273302595793 2209 18 3.11463164561669 2209 19 3.594966284732724 2209 20 3.726183205520808 2209 21 5.992219564749867 2209 22 4.647979056777629 2209 23 0.03391607962575094 2209 24 -3.4041541050589395 2209 25 -0.42226905627983413 2210 1 -2.680610099739944 2210 2 -13.87265215989324 2210 3 -7.914168547758409 2210 4 -2.887694347492868 2210 5 -1.328035761366472 2210 6 -2.0889885476166192 2210 7 -2.068094987999796 2210 8 -20.859673881929766 2210 9 -13.504738544964306 2210 10 -7.935772746368336 2210 11 -10.501303403647423 2210 12 -5.596145394625456 2210 13 -5.637536276745314 2210 14 -4.807769372319903 2210 15 -6.7030587335418765 2210 16 -6.1832793043842464 2210 17 -5.765608369443708 2210 18 -6.120074361654336 2210 19 -11.242210975576715 2210 20 -8.491344424643216 2210 21 -6.72959546819143 2210 22 -10.565490875563052 2210 23 -8.81457129421631 2210 24 -11.425904160687304 2210 25 -9.268852374877644 2211 1 -0.5579094753928509 2211 2 4.344316752023859 2211 3 0.10548379887126913 2211 4 1.080862072591053 2211 5 0.39091022055646363 2211 6 -0.34459570507297244 2211 7 -20.83882998462825 2211 8 -13.523533985556709 2211 9 -9.596210023098184 2211 10 -7.641370994562902 2211 11 -9.878724189828633 2211 12 -5.356494987070402 2211 13 -6.903783887840314 2211 14 -5.36953239294397 2211 15 -5.66782172970915 2211 16 -11.776516912649246 2211 17 -8.09681454373682 2211 18 -6.24889966675288 2211 19 -9.017667880953368 2211 20 -7.111973645624353 2211 21 -4.851754112446354 2211 22 -6.694910568176052 2211 23 -8.884077074754991 2211 24 -22.068520478961073 2211 25 -21.841340574473058 2212 1 -0.38310591312376374 2212 2 -13.87265215989324 2212 3 -7.416844499312656 2212 4 -19.404889222745734 2212 5 -19.86815713695649 2212 6 -20.837471352741368 2212 7 -9.76422453269138 2212 8 -8.931409893407528 2212 9 -4.565882973789897 2212 10 -4.811546097334958 2212 11 -5.33437423703316 2212 12 -2.3532166885147845 2212 13 -5.704830750411958 2212 14 -3.1188187497209796 2212 15 -3.054449096439992 2212 16 -3.302404134718664 2212 17 -3.3654975003012204 2212 18 -2.4701383581096223 2212 19 -3.1249968481778154 2212 20 -3.2052778509157207 2212 21 -0.8602778745355444 2212 22 -2.395554891367226 2212 23 -2.4384840075015504 2212 24 -1.5584774373541848 2212 25 -1.8284280924070622 2213 1 -18.722417247413578 2213 2 -13.87265215989324 2213 3 -8.676256581933673 2213 4 -6.052494655197485 2213 5 -19.86815713695649 2213 6 -10.812453482834291 2213 7 -20.83882998462825 2213 8 -10.740302902658858 2213 9 -8.502166627926947 2213 10 -7.533925169084302 2213 11 -6.547132786642791 2213 12 -5.537540293303202 2213 13 -19.21272171167451 2213 14 -12.534642001213385 2213 15 -7.313706836391567 2213 16 -6.485678619567956 2213 17 -5.210843068923204 2213 18 -5.173459955930568 2213 19 -6.10548967816603 2213 20 -5.944113645698426 2213 21 -4.032206378498714 2213 22 -4.983503549098767 2213 23 -4.848991410309253 2213 24 -3.774739206623689 2213 25 -4.475364877546681 2214 1 -18.722417247413578 2214 2 -13.87265215989324 2214 3 -11.959638740642493 2214 4 -19.404889222745734 2214 5 -19.86815713695649 2214 6 -20.837471352741368 2214 7 -16.35775344972497 2214 8 -14.630069172089565 2214 9 -13.44452543161407 2214 10 -13.396961424104859 2214 11 -14.214426678531204 2214 12 -11.902739272990123 2214 13 -19.21272171167451 2214 14 -16.497114895765606 2214 15 -23.794764734890496 2214 16 -17.961410714642476 2214 17 -23.493187600731638 2214 18 -17.54682168072378 2214 19 -14.54712617089092 2214 20 -13.225561826574948 2214 21 -10.75292122287572 2214 22 -11.770536338950464 2214 23 -11.657748837066682 2214 24 -10.52016051260279 2214 25 -10.266067235532772 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 157672.0 3 9.0 4 709.0 5 2756.0 6 3922.0 7 3912.0 8 3395.0 9 3089.0 10 2944.0 11 2831.0 12 2688.0 13 2897.0 14 4278.0 15 8990.0 16 13255.0 17 13995.0 18 14235.0 19 16429.0 20 19851.0 21 23599.0 22 24578.0 23 26775.0 24 28319.0 25 28992.0 26 24145.0 27 19458.0 28 20319.0 29 23800.0 30 23308.0 31 26849.0 32 34187.0 33 43392.0 34 52527.0 35 60298.0 36 59394.0 37 61281.0 38 43371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.802728657123225 18.97854440291257 12.575522609472062 25.643204330492146 2 13.61431827009339 20.067954724348553 42.418768840507475 23.89895816505058 3 18.508603401110616 25.03106491221091 29.179605504866252 27.280726181812227 4 11.700828502850975 17.074012836103748 38.82480214523288 32.4003565158124 5 12.35981204417852 37.6827259952566 36.22320707568464 13.73425488488024 6 31.61464647051457 37.93465814100901 18.023038898635818 12.427656489840603 7 27.394205550472705 31.31369319914608 23.06410491003355 18.227996340347666 8 24.18200219622388 37.87542224734165 20.42930321869873 17.51327233773574 9 25.859702880116973 15.852791309393192 20.33033536094929 37.95717044954054 10 13.765763579179435 30.010326457642982 35.20355706575785 21.020352897419734 11 32.91772441535611 22.68468613977615 24.939109957877317 19.458479486990424 12 22.91335986982805 25.15359374761705 32.18283199067117 19.750214391883727 13 27.71672211287423 21.845106418690772 25.697710860594203 24.7404606078408 14 19.939572279767965 22.649929951106433 28.294351639819283 29.11614612930632 15 22.12503277436418 31.776427537305775 23.873188860136114 22.225350828193932 16 21.283218494113942 28.995486653291252 28.114830855003746 21.606463997591057 17 20.809481751616854 28.89632183253084 29.11319410809425 21.181002307758057 18 20.791225347995233 28.216510378016146 30.599743641637993 20.392520632350628 19 22.22061148738626 27.586268897571877 27.806639898501228 22.386479716540634 20 22.367678177679778 28.28604008108685 28.726927590867064 20.619354150366316 21 21.651281150469888 26.930508623439298 27.21017524233921 24.2080349837516 22 21.69525061508575 28.621690185865074 28.494171035482513 21.188888163566663 23 21.429839353865272 28.24065856039762 28.58846631756457 21.74103576817254 24 21.75274112550747 27.852216102487603 28.959477746652706 21.435565025352222 25 21.976561381864414 27.793279509348928 28.661549061223766 21.568610047562885 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 964.0 1 964.0 2 2010.0 3 3056.0 4 3056.0 5 3056.0 6 4360.0 7 5664.0 8 5664.0 9 5664.0 10 6787.5 11 7911.0 12 7911.0 13 7911.0 14 9948.0 15 11985.0 16 11985.0 17 11985.0 18 16777.0 19 21569.0 20 21569.0 21 21569.0 22 29817.0 23 38065.0 24 38065.0 25 38065.0 26 48704.0 27 59343.0 28 59343.0 29 59343.0 30 71323.0 31 83303.0 32 83303.0 33 83303.0 34 91265.0 35 99227.0 36 99227.0 37 99227.0 38 104111.0 39 108995.0 40 108995.0 41 108995.0 42 110185.0 43 111375.0 44 111375.0 45 111375.0 46 109122.5 47 106870.0 48 106870.0 49 106870.0 50 98296.5 51 89723.0 52 89723.0 53 89723.0 54 77322.0 55 64921.0 56 64921.0 57 64921.0 58 53928.5 59 42936.0 60 42936.0 61 42936.0 62 34061.5 63 25187.0 64 25187.0 65 25187.0 66 18944.0 67 12701.0 68 12701.0 69 12701.0 70 9111.0 71 5521.0 72 5521.0 73 5521.0 74 3840.5 75 2160.0 76 2160.0 77 2160.0 78 1420.5 79 681.0 80 681.0 81 681.0 82 449.0 83 217.0 84 217.0 85 217.0 86 138.5 87 60.0 88 60.0 89 60.0 90 37.0 91 14.0 92 14.0 93 14.0 94 7.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.75440495806411 2 35.52455595828684 3 5.295257682151568 4 13.345463289338234 5 12.864328067292446 6 14.166562320973263 7 9.163841945639033 8 7.264454833458734 9 0.11767978024242923 10 1.3853414431175612 11 5.323292507388229 12 5.5446900600477145 13 15.496498971132993 14 1.5273993322614354 15 2.796723138925302 16 2.849579311407071 17 2.7673585986576525 18 1.4481704783317395 19 1.3946494483344765 20 0.2961940231525549 21 4.114692353806143 22 1.546347771453013 23 0.120671639062152 24 8.78132725505818 25 0.12366349788187478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 902449.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.55823643554541 #Duplication Level Percentage of deduplicated Percentage of total 1 96.84126618357325 90.60298078320336 2 2.4331382986078247 4.552802564430632 3 0.35051358339505784 0.9838029812743527 4 0.1409179019133113 0.5273612154082631 5 0.06618965809153678 0.3096293840657955 6 0.040234931484280145 0.22585855396645513 7 0.021749390996272527 0.14243842656108716 8 0.017355493171271438 0.12989994668586458 9 0.014072822434357788 0.11849656037661843 >10 0.06474379673480082 1.1064311004714558 >50 0.005455455243818273 0.36768424212212786 >100 0.003998944106052196 0.7269538992313083 >500 3.635402481234254E-4 0.2056603422027055 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.1080958591565839E-4 0.0 12 0.0 0.0 0.0 2.2161917183131677E-4 0.0 13 0.0 0.0 0.0 2.2161917183131677E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTGA 25 0.00792301 18.121576 13 TGTAGGA 400 0.0 16.314241 2 TAGGACC 210 0.0 15.792262 4 ATAGCCC 90 5.999027E-9 15.729543 3 GTAGGAC 435 0.0 14.519578 3 GTCCTAG 65 8.698273E-4 14.291686 1 GATTAAG 65 8.698273E-4 14.291686 1 TATAGCC 105 7.5216667E-7 14.242592 2 GTATTGG 75 1.5939873E-4 14.155575 1 GTACTAA 75 1.5939873E-4 14.155575 1 GTCCTAA 170 3.6379788E-12 14.051491 1 CTGTAGG 435 0.0 14.033545 1 CTCTAGG 95 5.370548E-6 13.969317 1 TAGCCCT 115 9.0039975E-10 13.953892 4 CTAGGAC 125 1.6189006E-10 13.938793 3 GTATAGC 105 9.878459E-7 13.902797 1 AGGATAG 45 0.0064234054 13.832745 5 TAGGACA 185 0.0 13.300197 4 GTATAAC 60 0.008447615 13.270851 1 GTAATAG 50 0.009356954 13.067619 3 >>END_MODULE