FastQCFastQC Report
Thu 26 May 2016
SRR1926631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902449
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17440.19325191783690823No Hit
CTGTAGGACGTGGAATATGGCAAGA15480.1715332389974392No Hit
GTCCTACAGTGGACATTTCTAAATT13900.15402532442276518No Hit
GTCCTACAGTGTGCATTTCTCATTT11310.12532564167060964No Hit
GTCCTAAAGTGTGTATTTCTCATTT11080.1227770211945495No Hit
CTTTAGGACGTGAAATATGGCGAGG10270.11380144473538117No Hit
GTACATGGGAAGCAGTGGTATCAAC10270.11380144473538117No Hit
GGTATCAACGCAGAGTACTTTTTTT9930.11003391881424877No Hit
CCCATGTACTCTGCGTTGATACCAC9560.10593396413536942No Hit
CTGTAGGACCTGGAATATGGCGAGA9460.10482586827621283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG957.090421E-813.0389141
TAGGACC4100.012.7841854
GACGTGG4100.012.743017
CTCGAAC751.5112611E-512.63840618
TGGACCG550.002995067312.1291295
GGACGTG7100.012.0803196
GTCCTAA3700.011.8461661
AGGACGT7350.011.7990855
GTAGGAC8300.011.5980753
TGTAGGA9050.011.5860432
CTGTAGG9150.011.5590881
GTCCTAG1251.7480488E-811.4341241
TCCAACG752.1154039E-411.37456518
TCGAACT752.1164928E-411.37393519
GGCGAGG2750.011.37393419
ATAGGAC1354.478352E-911.2961083
ACGTGGA4600.011.1532498
ATAAGCT600.005736030611.1183674
TAGGACG7550.011.1078494
CCCGTTT600.005941369611.06596412