FastQCFastQC Report
Thu 26 May 2016
SRR1926630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713118
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC250.002797227121.6933773
AGTCGGC250.00412761420.2854217
GTCCAGT602.4192468E-517.6480461
GATATAC1257.2759576E-1216.9421251
GAATAGA500.002560473515.8832431
TGTAGGA3850.015.829362
CCTACAC804.467165E-714.9141973
GTCCTAC4150.014.3523281
GCCGGTT400.00655461213.7902311
CTGTAGG4350.013.6924511
AATGCCG500.001251288913.6205748
GACAGTT450.00745746713.5236157
GTAGGAC3700.013.4850713
TAGGACA1355.4569682E-1113.4176454
TTAGACA808.788236E-613.3189884
GTTTTAA802.8146384E-413.2360351
ATATTGG600.00859157213.2360351
TAGGACC1553.6379788E-1213.06124
TTAGACC500.00960091613.0160263
TCCTACA5000.013.0011812
Produced by FastQC (version 0.11.5)