##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926630_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713118 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.31414857008237 27.0 7.0 31.0 2.0 31.0 2 16.559412327272625 20.0 2.0 30.0 2.0 31.0 3 19.95358411931826 24.0 15.0 31.0 2.0 31.0 4 22.071180365661785 28.0 7.0 35.0 2.0 37.0 5 22.671343312046535 29.0 7.0 35.0 2.0 37.0 6 23.60806907131779 31.0 2.0 35.0 2.0 37.0 7 23.488442305481 31.0 7.0 35.0 2.0 37.0 8 23.569104972809548 31.0 10.0 35.0 2.0 37.0 9 26.253218962359664 32.0 17.0 37.0 2.0 39.0 10 26.949213173696357 32.0 23.0 37.0 2.0 39.0 11 24.090234715713247 30.0 7.0 37.0 2.0 39.0 12 25.18136128943597 31.0 11.0 37.0 2.0 39.0 13 21.898008464237336 27.0 2.0 35.0 2.0 39.0 14 25.19600823426137 31.0 17.0 38.0 2.0 40.0 15 26.260349619558053 31.0 17.0 38.0 2.0 40.0 16 26.634967845433714 31.0 17.0 39.0 2.0 40.0 17 25.916121876042954 31.0 11.0 38.0 2.0 40.0 18 25.962715847868093 31.0 16.0 38.0 2.0 40.0 19 26.359063997823643 31.0 17.0 39.0 2.0 40.0 20 26.62686259497026 31.0 17.0 39.0 2.0 40.0 21 24.456911759344177 30.0 2.0 37.0 2.0 40.0 22 25.410535142851533 31.0 2.0 38.0 2.0 40.0 23 25.39402174675159 31.0 2.0 38.0 2.0 40.0 24 24.506418292624783 31.0 2.0 39.0 2.0 40.0 25 24.28985525537148 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.680123876371635 1101 2 10.308121822240281 1101 3 6.832946617297669 1101 4 8.232985273472025 1101 5 8.067519422708141 1101 6 7.407094586448398 1101 7 7.2963651033034935 1101 8 5.905568794499661 1101 9 5.679260732565972 1101 10 5.436323851298667 1101 11 8.193456922603762 1101 12 5.641543464919231 1101 13 10.110784918381352 1101 14 7.53738913047448 1101 15 6.977767265136883 1101 16 6.294326389024839 1101 17 2.7656188347803834 1101 18 4.775007450509083 1101 19 5.355706103471668 1101 20 3.477496351281367 1101 21 0.8395945887921457 1101 22 4.365687784441992 1101 23 5.6194299545446995 1101 24 7.067690532792792 1101 25 7.431790229190817 1102 1 5.65057795449875 1102 2 10.501324442191834 1102 3 6.559704828468572 1102 4 7.93680887811211 1102 5 8.036272087044402 1102 6 6.741873139356475 1102 7 6.718901250510264 1102 8 6.9574838567134805 1102 9 5.979771532844818 1102 10 5.3188422947746155 1102 11 7.967789522043063 1102 12 5.405005572415483 1102 13 9.612877519425524 1102 14 7.0462710872131815 1102 15 5.068144058705361 1102 16 5.60307469011866 1102 17 6.599682718667982 1102 18 6.719134124966061 1102 19 5.208673609853538 1102 20 5.520427898527256 1102 21 4.625922343527769 1102 22 5.720495877002914 1102 23 6.025892317817231 1102 24 7.6961642003739 1102 25 7.365136685686281 1103 1 5.133133970008995 1103 2 9.136671218071843 1103 3 6.1869538263244515 1103 4 8.020026522903354 1103 5 7.448830445720034 1103 6 7.042348827125906 1103 7 6.977609385325419 1103 8 6.609043825370495 1103 9 5.711927572542574 1103 10 4.898546451247672 1103 11 5.550086277640833 1103 12 4.6073605796087485 1103 13 8.563225088958326 1103 14 6.944761635186342 1103 15 5.901235096979583 1103 16 5.681365388759875 1103 17 3.342600942195883 1103 18 2.0594977943126693 1103 19 4.169192532803706 1103 20 4.623300417732395 1103 21 6.973762726093611 1103 22 2.3972322759356395 1103 23 4.908334702403739 1103 24 6.246115195463329 1103 25 1.8336047342326367 1104 1 5.48516605846498 1104 2 10.062644464265023 1104 3 6.707557933173522 1104 4 7.3583777160244495 1104 5 7.254033931494092 1104 6 7.00918853212157 1104 7 6.91002310637538 1104 8 6.895781847270499 1104 9 5.619561231434684 1104 10 4.563001676321278 1104 11 7.7602554683425815 1104 12 4.714054821636669 1104 13 8.992809734030448 1104 14 6.601055652650459 1104 15 5.935769334778051 1104 16 5.549421564956866 1104 17 5.8023361734122645 1104 18 3.2599480548142346 1104 19 4.431107065128181 1104 20 3.4493050071922475 1104 21 3.0695233289920445 1104 22 4.964745546473349 1104 23 5.234915806567862 1104 24 6.220000212248969 1104 25 5.28733817044909 1105 1 3.1782266043807788 1105 2 9.313395149324833 1105 3 6.012426956885495 1105 4 5.298647648384229 1105 5 2.1526623329924455 1105 6 4.669764888176438 1105 7 5.66870335490097 1105 8 2.837881275991929 1105 9 3.4075078759510333 1105 10 3.1220203416208037 1105 11 6.393820102434066 1105 12 3.6896317538380856 1105 13 4.853664489026439 1105 14 3.3369513078892936 1105 15 3.907978736627019 1105 16 4.109647365107342 1105 17 4.7830509771742875 1105 18 2.3594963189493896 1105 19 2.257824366963039 1105 20 1.59967124537431 1105 21 2.2250711889003156 1105 22 3.874739748035907 1105 23 1.2089627153721807 1105 24 4.167689186775899 1105 25 4.912539128516098 1106 1 6.425749876729498 1106 2 11.09445212355481 1106 3 7.742505687740021 1106 4 9.021424139197958 1106 5 8.729125779898094 1106 6 7.843623182282986 1106 7 7.511480954854676 1106 8 7.272322965670238 1106 9 6.560472142957192 1106 10 5.985122931643488 1106 11 8.257396488120616 1106 12 5.903604747485247 1106 13 4.729975489464671 1106 14 5.947389400096199 1106 15 6.0532772791327005 1106 16 6.216183273815481 1106 17 6.53370003503824 1106 18 6.436039635989218 1106 19 6.492487040725788 1106 20 6.118121849162382 1106 21 6.394621318109095 1106 22 6.570379079827294 1106 23 6.9013053697008715 1106 24 7.647816593202762 1106 25 6.66622325147873 1107 1 4.097842470015664 1107 2 -13.79676776766508 1107 3 -5.4477887581014794 1107 4 1.6009276077603403 1107 5 4.499122849053542 1107 6 4.991264030765457 1107 7 4.689443262885842 1107 8 5.305258405644697 1107 9 3.563754926653136 1107 10 3.2235373269056247 1107 11 5.610495484677912 1107 12 2.872149592082117 1107 13 4.500710917430396 1107 14 4.080865292562148 1107 15 3.866668087932844 1107 16 3.2764373952784744 1107 17 4.079326388621748 1107 18 -0.9454778518786924 1107 19 1.8621872206784573 1107 20 2.123609008436844 1107 21 -1.121446903391412 1107 22 2.013538233009399 1107 23 -0.756917135499446 1107 24 2.7091461503242087 1107 25 3.471893989819705 1108 1 5.188699541293303 1108 2 10.509150756547065 1108 3 0.3708681869710837 1108 4 6.020184879695822 1108 5 7.174606424456371 1108 6 7.21124548226501 1108 7 7.375137923046616 1108 8 6.944907619038901 1108 9 5.767853710062049 1108 10 4.746249242347048 1108 11 7.107473561103145 1108 12 2.5633447000768506 1108 13 3.6270130321594003 1108 14 4.951245736773444 1108 15 4.952563405936122 1108 16 4.891607175219395 1108 17 0.24537839852290588 1108 18 3.774509774613083 1108 19 4.791978735298191 1108 20 5.130435609362387 1108 21 4.968141059960889 1108 22 1.7942424996937874 1108 23 4.563866653322876 1108 24 5.430914526954041 1108 25 6.012501620816284 1109 1 3.3219802379227588 1109 2 -13.79676776766508 1109 3 -5.63948531749913 1109 4 1.9755702038139304 1109 5 1.1646337545651804 1109 6 2.3414167691120227 1109 7 3.310814899283425 1109 8 3.884711676162823 1109 9 3.331556055838302 1109 10 2.42119203795167 1109 11 0.6494398208069825 1109 12 1.3133442248257126 1109 13 2.1651779164230724 1109 14 3.013855955433325 1109 15 3.0645908716499193 1109 16 2.9746474898229742 1109 17 3.619195102396457 1109 18 3.5882081599631803 1109 19 1.8558562903275195 1109 20 0.8093540391579701 1109 21 0.7057130842709363 1109 22 2.5457514220586646 1109 23 2.8814358007793643 1109 24 4.114194494865082 1109 25 4.412683990519849 1110 1 2.8750138772964284 1110 2 8.701603567839804 1110 3 5.992594270890493 1110 4 7.0950702722903465 1110 5 7.8173267573498855 1110 6 3.1909754319153656 1110 7 5.906464364496063 1110 8 6.871559505460521 1110 9 5.4750020574871066 1110 10 4.759477603392298 1110 11 2.9079859457133317 1110 12 5.3302578988940965 1110 13 9.689392384549318 1110 14 7.263524314765316 1110 15 6.44745458210479 1110 16 6.102650046275734 1110 17 6.509926215661814 1110 18 6.606314800564057 1110 19 5.746693474899651 1110 20 5.914004124686354 1110 21 3.28324382480978 1110 22 5.681155473176979 1110 23 6.1579630261956595 1110 24 7.364497939362938 1110 25 7.6350470444067575 1111 1 5.135077470341688 1111 2 9.525358893789182 1111 3 7.569156146134027 1111 4 8.32997424602797 1111 5 8.814540582279278 1111 6 7.764196571191011 1111 7 7.590731389069926 1111 8 7.958000332348384 1111 9 6.4796932224597406 1111 10 5.48563657836511 1111 11 7.497896757549736 1111 12 6.585578754713357 1111 13 8.397105683569063 1111 14 6.301899801518253 1111 15 5.643706743682571 1111 16 5.4306470308906505 1111 17 6.6965980353091865 1111 18 6.673457417582135 1111 19 5.5979762869499865 1111 20 4.950124439569979 1111 21 7.757561479329162 1111 22 7.2511581218260375 1111 23 7.12469764938713 1111 24 7.308908780487286 1111 25 7.910942045845687 1112 1 -2.646982554509158 1112 2 7.379165842293425 1112 3 5.373983569010434 1112 4 -0.4449536508312839 1112 5 3.85320603343893 1112 6 2.5508737924381606 1112 7 5.332189153986111 1112 8 5.71697761945352 1112 9 4.079151435080469 1112 10 2.754507811401812 1112 11 -0.7665467424616175 1112 12 3.380168018239388 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23 1.5265244455925924 2208 24 2.913475946306061 2208 25 4.262844866037565 2209 1 3.1478047065013683 2209 2 4.939566637479615 2209 3 5.125573941387053 2209 4 0.5545160026756726 2209 5 4.633321709013636 2209 6 5.2742677431526275 2209 7 5.846944794337809 2209 8 4.315650752567155 2209 9 4.099600128226655 2209 10 3.1142174883907785 2209 11 -0.5490953408792514 2209 12 2.719071435138428 2209 13 1.9531627166711907 2209 14 3.4139296479869294 2209 15 3.9911640618747874 2209 16 3.380960529428748 2209 17 1.4509931741963307 2209 18 3.2654473665223485 2209 19 3.770882546613258 2209 20 3.9067720164299153 2209 21 5.893678798677829 2209 22 4.489672184007858 2209 23 0.05623957447430428 2209 24 -3.1471907395859198 2209 25 0.25362272231871685 2210 1 -2.5067966203739616 2210 2 -13.79676776766508 2210 3 -7.670451537875433 2210 4 -2.560344865355855 2210 5 -0.9610594216795114 2210 6 -1.3612119263495899 2210 7 -1.3616358018063863 2210 8 -20.72534603199876 2210 9 -13.043141402428505 2210 10 -7.2186735531656545 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2214 22 -12.568543300474397 2214 23 -12.552302325286135 2214 24 -11.381548677541819 2214 25 -11.108794833884183 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 128070.0 3 11.0 4 486.0 5 2313.0 6 3232.0 7 3241.0 8 2840.0 9 2551.0 10 2515.0 11 2324.0 12 2227.0 13 2378.0 14 3575.0 15 7032.0 16 10339.0 17 10875.0 18 11287.0 19 13128.0 20 15699.0 21 18219.0 22 19130.0 23 21070.0 24 22308.0 25 22930.0 26 19131.0 27 15350.0 28 16503.0 29 19167.0 30 18568.0 31 21332.0 32 27309.0 33 33616.0 34 41749.0 35 46475.0 36 46282.0 37 47077.0 38 32779.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50633207841351 19.206529218992866 12.73473604175723 25.5524026608364 2 13.723078925108986 20.274795584184613 42.096644761099185 23.905480729607216 3 18.631056003600147 25.519297497661086 29.152455560687347 26.697190938051417 4 12.132912567291628 17.188238997263944 38.36546920525258 32.31337923019185 5 12.740016917350557 37.45186821159208 35.795605019991044 14.012509851066323 6 31.582987670217015 37.4223989699375 18.05380120771569 12.940812152129801 7 27.071942401696386 31.499380497697672 23.121151226367992 18.30752587423795 8 24.30551358147069 37.49410683848507 20.425093383944006 17.775286196100236 9 25.83790431612154 15.932150563750644 20.39161400735382 37.83833111277399 10 14.418737576672724 29.81996661519606 34.547869088344605 21.21342671978661 11 32.74791537403321 22.87779819665601 24.807604312743504 19.56668211656728 12 23.268920856049835 25.068335000281948 31.785346482936284 19.877397660731937 13 27.809876559570114 21.836466277505746 25.61339225962908 24.74026490329506 14 20.236045910630608 22.705144798461102 27.936798130769265 29.122011160139028 15 22.175088051920692 31.588599494702457 23.861529426872536 22.374783026504314 16 21.515716392198506 28.713399836675336 27.986662780732306 21.784220990393855 17 20.996666623394795 28.881090335804156 28.885706002241484 21.236537038559568 18 20.969412434201168 27.9267321098307 30.607767819035423 20.496087636932707 19 22.511684917462606 27.422123064541125 27.596028194529172 22.470163823467097 20 22.825931020514943 28.167888899203085 28.295316134875964 20.710863945406008 21 21.751866877656262 26.649769949192848 27.088957853756128 24.509405319394762 22 22.10920389720981 28.38022573286284 28.10866847230173 21.40190189762562 23 21.64284400591343 28.163621737048928 28.363403563501276 21.83013069353636 24 22.201037440264287 27.68467219620178 28.530221319566294 21.58406904396763 25 22.072384493128837 27.690339935865392 28.523893228801043 21.713382342204728 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 835.0 1 835.0 2 1702.5 3 2570.0 4 2570.0 5 2570.0 6 3561.5 7 4553.0 8 4553.0 9 4553.0 10 5506.5 11 6460.0 12 6460.0 13 6460.0 14 7978.5 15 9497.0 16 9497.0 17 9497.0 18 13152.0 19 16807.0 20 16807.0 21 16807.0 22 23092.0 23 29377.0 24 29377.0 25 29377.0 26 37760.0 27 46143.0 28 46143.0 29 46143.0 30 55226.5 31 64310.0 32 64310.0 33 64310.0 34 70664.5 35 77019.0 36 77019.0 37 77019.0 38 80562.5 39 84106.0 40 84106.0 41 84106.0 42 85018.0 43 85930.0 44 85930.0 45 85930.0 46 85099.0 47 84268.0 48 84268.0 49 84268.0 50 77933.0 51 71598.0 52 71598.0 53 71598.0 54 62380.0 55 53162.0 56 53162.0 57 53162.0 58 44572.0 59 35982.0 60 35982.0 61 35982.0 62 28684.5 63 21387.0 64 21387.0 65 21387.0 66 16179.0 67 10971.0 68 10971.0 69 10971.0 70 8064.5 71 5158.0 72 5158.0 73 5158.0 74 3594.0 75 2030.0 76 2030.0 77 2030.0 78 1353.5 79 677.0 80 677.0 81 677.0 82 439.5 83 202.0 84 202.0 85 202.0 86 134.0 87 66.0 88 66.0 89 66.0 90 37.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.579264581738226 2 35.34450119054631 3 5.271217386182932 4 13.12728047812564 5 12.633112612498914 6 13.961644496422752 7 9.00411993527018 8 7.198528153825874 9 0.11709142105514095 10 1.3748075353588045 11 5.303049425200317 12 5.50147380938358 13 15.34472555734114 14 1.51405517740402 15 2.813138919505608 16 2.80738952038793 17 2.7803252757608137 18 1.432862443522671 19 1.3828006024248443 20 0.29784692014505315 21 4.116569768257147 22 1.524572370911967 23 0.11793279653577668 24 8.653126130598302 25 0.1214385277050923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 713118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.45060968570942 #Duplication Level Percentage of deduplicated Percentage of total 1 97.0290619515328 90.6742499660321 2 2.2237893765107817 4.156289460950724 3 0.369293152081383 1.0353201064428794 4 0.13398331290180907 0.5008328911354093 5 0.06210108156614128 0.29016919672489366 6 0.04075337713993265 0.22850567642870154 7 0.024126860926722175 0.15782689043831552 8 0.021452395251378417 0.16037915323681046 9 0.012660158126839616 0.1064789546103586 >10 0.07081855888975133 1.2070786833028448 >50 0.006746669188365899 0.4629686493010239 >100 0.004906452599431837 0.8684484624643388 >500 3.0665328463112633E-4 0.15145190893162838 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.402292467726239E-4 0.0 0.0 0.0 0.0 7 1.402292467726239E-4 0.0 0.0 0.0 0.0 8 1.402292467726239E-4 0.0 0.0 0.0 0.0 9 1.402292467726239E-4 0.0 0.0 0.0 0.0 10 1.402292467726239E-4 0.0 0.0 0.0 0.0 11 1.402292467726239E-4 0.0 0.0 0.0 0.0 12 1.402292467726239E-4 0.0 0.0 0.0 0.0 13 1.402292467726239E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAATAC 25 0.0027972271 21.693377 3 AGTCGGC 25 0.004127614 20.285421 7 GTCCAGT 60 2.4192468E-5 17.648046 1 GATATAC 125 7.2759576E-12 16.942125 1 GAATAGA 50 0.0025604735 15.883243 1 TGTAGGA 385 0.0 15.82936 2 CCTACAC 80 4.467165E-7 14.914197 3 GTCCTAC 415 0.0 14.352328 1 GCCGGTT 40 0.006554612 13.79023 11 CTGTAGG 435 0.0 13.692451 1 AATGCCG 50 0.0012512889 13.620574 8 GACAGTT 45 0.007457467 13.523615 7 GTAGGAC 370 0.0 13.485071 3 TAGGACA 135 5.4569682E-11 13.417645 4 TTAGACA 80 8.788236E-6 13.318988 4 GTTTTAA 80 2.8146384E-4 13.236035 1 ATATTGG 60 0.008591572 13.236035 1 TAGGACC 155 3.6379788E-12 13.0612 4 TTAGACC 50 0.009600916 13.016026 3 TCCTACA 500 0.0 13.001181 2 >>END_MODULE