FastQCFastQC Report
Thu 26 May 2016
SRR1926630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713118
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18810.2637712131793055No Hit
CTGTAGGACGTGGAATATGGCAAGA12260.1719210565432369No Hit
GTCCTACAGTGGACATTTCTAAATT11520.1615440922820627No Hit
GGTATCAACGCAGAGTACTTTTTTT10780.15116712802088855No Hit
CTTTAGGACGTGAAATATGGCGAGG10380.1455579581499836No Hit
CCCATGTACTCTGCGTTGATACCAC10100.14163153924035013No Hit
GTACATGGGAAGCAGTGGTATCAAC9980.13994878827907864No Hit
GTCCTAAAGTGTGTATTTCTCATTT9240.12957182401790449No Hit
GTCCTACAGTGTGCATTTCTCATTT8510.11933508900350293No Hit
TATCAACGCAGAGTACTTTTTTTTT8270.11596958708095995No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8000.11218339741809912No Hit
CTGTAGGACCTGGAATATGGCGAGA7180.10068459918274396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG851.7687853E-814.49300618
GGACCGT400.005152919314.3005266
GTTTTAG500.00146104213.3443331
CCAACGA701.1151141E-412.18368119
ATCTAGG550.002990151812.1312111
GATATAC2250.011.86162951
ACTGTTC2500.011.7785628
CTATAGT907.1511968E-611.6531024
GGCGAGG2700.011.58201719
TAGGACC2250.011.4412284
GTCCTAA3450.011.3274881
TGTAGGA7250.011.3129112
CTGTAGG6800.011.2137261
GTCTAAA951.3080438E-511.0366671
TCCTACA7750.010.9522232
CCTACAC1407.84712E-910.8971763
GTAGGAC6750.010.8769963
TATAGTG1158.3897066E-710.7779675
ATTATAC803.6393083E-410.7269083
GTCCTAC7200.010.7231251
Produced by FastQC (version 0.11.5)