##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926630_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713118 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.220848443034672 31.0 28.0 31.0 20.0 34.0 2 28.63734613345898 31.0 28.0 33.0 22.0 34.0 3 29.026347673176108 31.0 28.0 33.0 24.0 34.0 4 32.78800002243668 35.0 33.0 37.0 26.0 37.0 5 32.468928003500125 35.0 33.0 37.0 25.0 37.0 6 32.56632282455358 35.0 33.0 37.0 25.0 37.0 7 32.41049587866244 35.0 33.0 37.0 23.0 37.0 8 32.3900153971713 35.0 33.0 37.0 24.0 37.0 9 33.59374605605243 37.0 33.0 39.0 22.0 39.0 10 33.48145608440679 37.0 33.0 39.0 21.0 39.0 11 33.72246949312736 37.0 34.0 39.0 25.0 39.0 12 33.51607447855755 37.0 33.0 39.0 22.0 39.0 13 33.65215013504076 37.0 33.0 39.0 23.0 39.0 14 34.296424995582775 38.0 33.0 40.0 19.0 41.0 15 34.4161415081375 38.0 33.0 40.0 22.0 41.0 16 34.39104327755014 38.0 34.0 40.0 22.0 41.0 17 34.31329878084693 38.0 33.0 40.0 19.0 41.0 18 34.27614644420699 38.0 33.0 40.0 19.0 41.0 19 34.316250606491494 39.0 34.0 40.0 18.0 41.0 20 34.29593699780401 39.0 34.0 40.0 18.0 41.0 21 34.23130533796651 39.0 34.0 40.0 18.0 41.0 22 34.145191959815904 39.0 34.0 40.0 18.0 41.0 23 33.80320507966424 39.0 33.0 40.0 11.0 41.0 24 33.87691377864533 39.0 34.0 40.0 11.0 41.0 25 33.67109791086468 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3192145314429915 1101 2 0.6437305522058807 1101 3 0.5386134185230951 1101 4 0.36146786313971546 1101 5 0.3700138054695259 1101 6 -0.02294302741909604 1101 7 0.49116915790647653 1101 8 0.7003132697639742 1101 9 1.0358491841726902 1101 10 1.0598285500590023 1101 11 0.6705476522045757 1101 12 0.8821800444393446 1101 13 0.6925445917512363 1101 14 1.0327020262085043 1101 15 0.7208866926364905 1101 16 0.6535142776476235 1101 17 0.8050147175284934 1101 18 0.8008725282157556 1101 19 0.8050604919167412 1101 20 0.9828522693346855 1101 21 0.7967816118296724 1101 22 0.7644824285761658 1101 23 0.8637987270410648 1101 24 0.9547825614767547 1101 25 1.0930508124396354 1102 1 0.14403912408164743 1102 2 0.23106686792408482 1102 3 0.04857114020840925 1102 4 -0.2578806094746682 1102 5 -0.05158700298413521 1102 6 -0.4874289923225774 1102 7 -0.2458113278678553 1102 8 0.04571155355527878 1102 9 0.08472627614683148 1102 10 0.18856551675291655 1102 11 -0.21179525852949865 1102 12 -0.24179747733925439 1102 13 -0.42863569040510896 1102 14 -0.07793876860375093 1102 15 -0.26078643530681944 1102 16 -0.29237650086516 1102 17 -0.07168606935971411 1102 18 -0.1937010024498136 1102 19 -0.11537929282996373 1102 20 -0.1518553181934621 1102 21 -0.2891266196528761 1102 22 -0.21591056415677912 1102 23 0.04112691810092883 1102 24 -0.13343141795080982 1102 25 -0.11520248632210439 1103 1 0.11539329730959835 1103 2 -0.019406174336037196 1103 3 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0.44412748086310216 2202 14 0.7660732874655025 2202 15 0.6814277506512738 2202 16 0.6700154142404315 2202 17 0.6842657364783449 2202 18 0.7527136093856654 2202 19 0.5083033293901948 2202 20 0.5954440495154003 2202 21 0.7990647566437943 2202 22 0.6601150738123067 2202 23 0.8342074771873413 2202 24 0.7146110392889184 2202 25 0.6089164280795529 2203 1 -0.18440270500633105 2203 2 -0.011038013964455473 2203 3 -0.34205820545372134 2203 4 -0.8034202380325297 2203 5 -0.37389008910763977 2203 6 -0.4645712137571465 2203 7 -0.4675563582090625 2203 8 -0.33215779619460406 2203 9 -0.17367010903843294 2203 10 -0.1277960992545104 2203 11 -0.39800465751351055 2203 12 -0.0780791234615208 2203 13 -0.2334397046811958 2203 14 -0.10881617355381223 2203 15 -0.10725382836876207 2203 16 -0.06709543556591058 2203 17 -0.04166502261058014 2203 18 0.07452862573290986 2203 19 0.1771828093095138 2203 20 -0.033423048753675744 2203 21 -0.22533634203500696 2203 22 -0.3275469456682387 2203 23 0.004407002857107045 2203 24 -0.14171493719494066 2203 25 -0.2206451471900266 2204 1 -0.14923537695255007 2204 2 -0.19960034497091428 2204 3 -0.1946038244934627 2204 4 -0.7040057082906301 2204 5 -0.6479236399966162 2204 6 -0.6765818498108516 2204 7 -0.40937288692378004 2204 8 -0.44385623701376176 2204 9 -0.2568606173799921 2204 10 -0.2911526244170375 2204 11 -0.44316492606937885 2204 12 -0.3560418844497448 2204 13 -0.34154171282302315 2204 14 0.004978316190680232 2204 15 -0.21607589247362569 2204 16 -0.44333910301675417 2204 17 -0.3589025724710879 2204 18 -0.2385149027080189 2204 19 -0.17682183675060514 2204 20 -0.24343124956914153 2204 21 -0.21259013322903542 2204 22 -0.4157906695437674 2204 23 -0.12872127803012035 2204 24 -0.28375383047598035 2204 25 -0.40387726873906615 2205 1 0.2690038186169197 2205 2 0.37691513027852963 2205 3 0.5327900599906954 2205 4 0.27628151321938077 2205 5 0.24897813112729494 2205 6 -0.037544251560277075 2205 7 -0.0251574151589935 2205 8 0.16270800512481287 2205 9 0.5141862659591183 2205 10 0.3807771003988094 2205 11 0.24438279695034026 2205 12 0.3962404145205696 2205 13 0.3586687728223197 2205 14 0.6728811198741482 2205 15 0.7130388023344167 2205 16 0.6534787917477587 2205 17 0.5581319702835401 2205 18 0.46213269370241505 2205 19 0.3852139623153832 2205 20 0.44737554686134473 2205 21 0.4152861625904123 2205 22 0.16471688024559228 2205 23 0.2426461939596436 2205 24 0.2210693658613394 2205 25 0.41063309689950955 2206 1 0.47303795360528156 2206 2 0.5056971318301215 2206 3 0.6592446759720758 2206 4 0.7068478437081254 2206 5 0.9215925609178299 2206 6 0.6001593946771209 2206 7 0.7436556182002008 2206 8 0.7523123495778776 2206 9 0.9951792838024076 2206 10 0.9952068909341065 2206 11 0.8634673586260604 2206 12 0.7624157442335218 2206 13 0.5720124500915205 2206 14 1.0351774736367503 2206 15 0.694419717772746 2206 16 0.7326099011348859 2206 17 0.7947495032548417 2206 18 0.8345143849359289 2206 19 0.7917306419119754 2206 20 0.9384539021755458 2206 21 0.9614459763995669 2206 22 1.0894647468088152 2206 23 1.0533521675826023 2206 24 0.8742889159001308 2206 25 1.1771187446884923 2207 1 -0.6972481677817512 2207 2 0.0365206867193244 2207 3 0.2905315546976297 2207 4 0.39128684411971193 2207 5 0.15140819728213728 2207 6 0.24372918456028003 2207 7 0.3353523333443249 2207 8 0.5430236018072918 2207 9 0.8099500304791363 2207 10 0.7939806300515997 2207 11 0.5762644109734723 2207 12 0.6895752818079117 2207 13 0.3878567595015312 2207 14 -5.128067429708764 2207 15 -1.903983534537879 2207 16 -0.7752338742622555 2207 17 -0.5652649913801184 2207 18 0.08150579458839502 2207 19 0.3253306945749941 2207 20 0.5126512371560281 2207 21 0.6320307881631066 2207 22 0.7972864972513136 2207 23 0.991915463795479 2207 24 0.375019492258744 2207 25 0.3509510068174393 2208 1 0.15448705552667974 2208 2 0.6840268181143188 2208 3 0.4969933160112241 2208 4 0.43544592729686826 2208 5 0.35104274361818 2208 6 0.4944436743298297 2208 7 0.6031647914854759 2208 8 0.8037682121658847 2208 9 0.7911468882651462 2208 10 0.8455851055846964 2208 11 0.5673426237473933 2208 12 0.5381935397923598 2208 13 0.5222258807413453 2208 14 0.9282945816021595 2208 15 0.7019132731064204 2208 16 0.6920151712078564 2208 17 0.6534654868390604 2208 18 0.8114962285453586 2208 19 0.5661910931719731 2208 20 0.47331192237868436 2208 21 0.69103264802947 2208 22 0.660103276114512 2208 23 1.030800184086516 2208 24 0.8843881689798252 2208 25 1.086233584409669 2209 1 -0.20883176269315484 2209 2 -0.3028242673950814 2209 3 0.2603693295458456 2209 4 0.5359558788010119 2209 5 0.39839229350547356 2209 6 0.6066582384678867 2209 7 0.8230891879307123 2209 8 0.8746110980780486 2209 9 0.8628344864944921 2209 10 0.6510525451090601 2209 11 0.7213758547059257 2209 12 0.6484879755053043 2209 13 0.6926510966656778 2209 14 1.151957100406925 2209 15 1.0425366059110601 2209 16 0.8727800023374925 2209 17 1.0415621325020652 2209 18 0.9701519463946227 2209 19 1.181433644563647 2209 20 0.8555941115654093 2209 21 0.7714004763858497 2209 22 1.1104611073066266 2209 23 1.3117000730603152 2209 24 1.2119082474191174 2209 25 1.1979074365381521 2210 1 -0.18422555161415133 2210 2 -0.33642691029195504 2210 3 0.46989827523621486 2210 4 0.5902736505187605 2210 5 0.8335558464922954 2210 6 0.7009525388735511 2210 7 0.8957193316077081 2210 8 0.9179855401256205 2210 9 1.383735486399523 2210 10 1.3507940893314512 2210 11 1.038484134787872 2210 12 0.8519700057059651 2210 13 0.7872350574835068 2210 14 1.1998372203412586 2210 15 0.9659990992970364 2210 16 1.106067885442215 2210 17 1.131526450680802 2210 18 0.9211762724423522 2210 19 0.8731786619975423 2210 20 0.9142988478862435 2210 21 0.9732354445706903 2210 22 1.223237370076781 2210 23 1.3019038528912716 2210 24 1.182902861227845 2210 25 1.0710626321098076 2211 1 -1.6074994056809082 2211 2 -0.5714877070260442 2211 3 -0.04062916849035503 2211 4 0.09648823260049966 2211 5 0.36842798187877435 2211 6 -0.1167535218283291 2211 7 0.02637838155553851 2211 8 0.10058302393833429 2211 9 0.43473799134442004 2211 10 0.18345252797435307 2211 11 0.011250851693965558 2211 12 0.04649566470321531 2211 13 0.08731551083286604 2211 14 0.30426988369844565 2211 15 0.11420049265067433 2211 16 0.0679730488608854 2211 17 -0.16106376071125084 2211 18 0.014725600182600829 2211 19 -0.04707836713078706 2211 20 -0.14095819880831328 2211 21 0.05974071268411052 2211 22 -0.26519315722641323 2211 23 0.0736475123592939 2211 24 -0.06929766084061129 2211 25 0.04418840282829706 2212 1 1.332055971298221 2212 2 0.5149615697394445 2212 3 0.8349630382624156 2212 4 0.9702731598897714 2212 5 0.9572017104899402 2212 6 1.0578409698420543 2212 7 1.4261824853708305 2212 8 1.3877750602904655 2212 9 1.5280024866939002 2212 10 1.2519274422899471 2212 11 1.2074641171252267 2212 12 1.4855290283730227 2212 13 1.3304349397325552 2212 14 1.3241822306444675 2212 15 1.0603274282147055 2212 16 0.48494630757939916 2212 17 1.1030822351965526 2212 18 1.0924474921460146 2212 19 1.2721606068508393 2212 20 1.3106559276625163 2212 21 1.1511301555902165 2212 22 1.3467581236829034 2212 23 1.8278115165160358 2212 24 1.6358221036966896 2212 25 1.011664633876073 2213 1 0.7973489174629407 2213 2 0.6962327162553557 2213 3 0.027920980786721117 2213 4 -0.7935550554513568 2213 5 -1.7434332203422152 2213 6 -0.32854602745975825 2213 7 0.14990727015818806 2213 8 0.16909867158003067 2213 9 -0.3208079548667655 2213 10 -0.11244576427136366 2213 11 0.008328179825980442 2213 12 -0.33212911544470103 2213 13 -0.38249709161782874 2213 14 -7.60264547075602 2213 15 -3.055352044139571 2213 16 -1.6392453066224988 2213 17 -0.7539084282151549 2213 18 -4.6688859696724805 2213 19 -2.462362284921827 2213 20 -4.800767473767948 2213 21 -1.7442133280707566 2213 22 -1.2046573544960353 2213 23 -3.948875921918159 2213 24 -1.5526870401638604 2213 25 -1.2821648663166556 2214 1 0.7632818224318534 2214 2 0.7776161832360771 2214 3 -1.445938397911675 2214 4 -1.5583500813437325 2214 5 0.08179467299389387 2214 6 -0.017254410586126312 2214 7 0.006344571346872385 2214 8 -0.09196115511134195 2214 9 -4.567538933927711 2214 10 -1.0071731870993759 2214 11 -0.2096065494442385 2214 12 -0.5175814671191858 2214 13 -0.6977721512251378 2214 14 -2.052552235103633 2214 15 -1.4769013147077032 2214 16 -1.0224957958931782 2214 17 -1.3836647454357518 2214 18 -0.4033029148914835 2214 19 -3.6456208565400843 2214 20 -1.2221348915156298 2214 21 -2.663360986787854 2214 22 -5.308244184887968 2214 23 -5.267912159700824 2214 24 -4.622506977006086 2214 25 -2.526448048683285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25055.0 3 12.0 4 448.0 5 2826.0 6 3616.0 7 2935.0 8 2167.0 9 1791.0 10 1526.0 11 1409.0 12 1412.0 13 1376.0 14 1434.0 15 1352.0 16 1508.0 17 1573.0 18 1744.0 19 2079.0 20 2602.0 21 3358.0 22 4387.0 23 5519.0 24 6559.0 25 7699.0 26 8824.0 27 9949.0 28 11495.0 29 13749.0 30 16791.0 31 21410.0 32 27351.0 33 35470.0 34 45837.0 35 59920.0 36 80798.0 37 117627.0 38 179510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.69653040057375 19.995143880095945 9.445293563676223 29.86303215565408 2 13.607115954084644 21.76378253190943 38.95861417888359 25.670487335122328 3 18.245244908264603 25.180384895920998 29.31478987824721 27.25958031756719 4 12.481133187074619 17.89513699129742 37.07812684109234 32.54560298053562 5 13.347574411503574 38.567046444335666 34.6554720384425 13.429907105718264 6 31.464517845604167 38.170151602817235 17.45494782098226 12.910382730596345 7 27.811403921546596 32.12162160263965 21.63522790003835 18.43174657577541 8 25.195600344487012 38.045162640861044 19.701369659011245 17.057867355640692 9 26.060530275675347 16.198122074962452 20.030544733141035 37.71080291622116 10 15.133358859244236 30.0145441591246 33.43824202210095 21.41385495953021 11 33.84644294022042 23.356513715409978 24.06258282464104 18.734460519728565 12 23.121010354847034 25.422103054848154 31.257782963303676 20.199103627001133 13 28.507343805653484 22.729337921634286 25.700374973005868 23.062943299706358 14 20.750014384491134 23.00929167958932 27.214334531339247 29.026359404580305 15 22.640953799294916 31.45507119558663 23.158127964145585 22.745847040972873 16 21.8317690431817 28.884607132489975 27.710723415831932 21.572900408496388 17 21.266289996648393 29.493290477652923 27.72423154969001 21.516187976008673 18 21.323296377216035 28.374080420923914 29.572653408086968 20.729969793773087 19 23.192700123964393 27.443809352861 27.43511501764109 21.928375505533523 20 23.021380963131964 28.105722671505557 28.059586796445434 20.81330956891704 21 22.436009799233506 27.233960205688547 27.381341350718046 22.9486886443599 22 22.695542673162088 28.56764238176571 27.128048934397953 21.60876601067425 23 22.01753426501645 28.25212657652731 27.713646268920435 22.016692889535815 24 22.687785280074042 27.545346823443623 28.21606613239099 21.550801764091347 25 22.394470480341262 27.81124582467418 27.896785665205474 21.897498029779083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 267.5 3 273.0 4 273.0 5 273.0 6 446.0 7 619.0 8 619.0 9 619.0 10 1085.0 11 1551.0 12 1551.0 13 1551.0 14 2457.0 15 3363.0 16 3363.0 17 3363.0 18 5808.0 19 8253.0 20 8253.0 21 8253.0 22 13125.5 23 17998.0 24 17998.0 25 17998.0 26 25574.0 27 33150.0 28 33150.0 29 33150.0 30 43640.0 31 54130.0 32 54130.0 33 54130.0 34 61430.5 35 68731.0 36 68731.0 37 68731.0 38 74705.5 39 80680.0 40 80680.0 41 80680.0 42 84723.5 43 88767.0 44 88767.0 45 88767.0 46 92015.5 47 95264.0 48 95264.0 49 95264.0 50 91519.5 51 87775.0 52 87775.0 53 87775.0 54 77230.0 55 66685.0 56 66685.0 57 66685.0 58 57116.0 59 47547.0 60 47547.0 61 47547.0 62 38740.5 63 29934.0 64 29934.0 65 29934.0 66 23053.5 67 16173.0 68 16173.0 69 16173.0 70 11856.5 71 7540.0 72 7540.0 73 7540.0 74 5267.0 75 2994.0 76 2994.0 77 2994.0 78 2001.5 79 1009.0 80 1009.0 81 1009.0 82 657.0 83 305.0 84 305.0 85 305.0 86 198.5 87 92.0 88 92.0 89 92.0 90 57.0 91 22.0 92 22.0 93 22.0 94 11.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08624098676516369 2 0.033795248472202354 3 0.02776539086097953 4 0.031691809770613 5 0.02201599174330195 6 0.3891361597940313 7 0.16897624236101177 8 0.1872060444414529 9 0.19281521431235785 10 0.016406821872396993 11 0.0021034387015893582 12 0.0030850434289977254 13 0.0 14 0.07642493949108002 15 0.0016827509612714867 16 0.0021034387015893582 17 0.0037861896628608445 18 0.002804584935452478 19 0.001402292467726239 20 9.816047274083673E-4 21 1.402292467726239E-4 22 0.0 23 0.0 24 4.2068774031787167E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 713118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.63238103242124 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90572209995578 76.86294369723319 2 6.072427816782142 10.157031939299957 3 1.0624850228024059 2.665744568047547 4 0.3826411604984573 1.2800476533397933 5 0.16389753160463919 0.6853570406716243 6 0.10927803256291382 0.5483509234664966 7 0.05822965657229708 0.3408919378088966 8 0.03820479301626431 0.2556126245440802 9 0.028190172749886604 0.21218501419094643 >10 0.14961944129950833 2.2373828847862627 >50 0.014820863781984784 0.8694762095705424 >100 0.012294055950311654 2.0517143983832833 >500 0.0011788820741447292 0.7931079673363503 >1k 0.0010104703492669108 1.0401531413210703 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1881 0.2637712131793055 No Hit CTGTAGGACGTGGAATATGGCAAGA 1226 0.1719210565432369 No Hit GTCCTACAGTGGACATTTCTAAATT 1152 0.1615440922820627 No Hit GGTATCAACGCAGAGTACTTTTTTT 1078 0.15116712802088855 No Hit CTTTAGGACGTGAAATATGGCGAGG 1038 0.1455579581499836 No Hit CCCATGTACTCTGCGTTGATACCAC 1010 0.14163153924035013 No Hit GTACATGGGAAGCAGTGGTATCAAC 998 0.13994878827907864 No Hit GTCCTAAAGTGTGTATTTCTCATTT 924 0.12957182401790449 No Hit GTCCTACAGTGTGCATTTCTCATTT 851 0.11933508900350293 No Hit TATCAACGCAGAGTACTTTTTTTTT 827 0.11596958708095995 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 800 0.11218339741809912 No Hit CTGTAGGACCTGGAATATGGCGAGA 718 0.10068459918274396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.402292467726239E-4 0.0 5 0.0 0.0 0.0 1.402292467726239E-4 0.0 6 1.402292467726239E-4 0.0 0.0 1.402292467726239E-4 0.0 7 1.402292467726239E-4 0.0 0.0 1.402292467726239E-4 0.0 8 1.402292467726239E-4 0.0 0.0 1.402292467726239E-4 0.0 9 1.402292467726239E-4 0.0 0.0 1.402292467726239E-4 0.0 10 1.402292467726239E-4 0.0 0.0 2.804584935452478E-4 0.0 11 1.402292467726239E-4 0.0 0.0 2.804584935452478E-4 0.0 12 1.402292467726239E-4 0.0 0.0 2.804584935452478E-4 0.0 13 1.402292467726239E-4 0.0 0.0 2.804584935452478E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 85 1.7687853E-8 14.493006 18 GGACCGT 40 0.0051529193 14.300526 6 GTTTTAG 50 0.001461042 13.344333 1 CCAACGA 70 1.1151141E-4 12.183681 19 ATCTAGG 55 0.0029901518 12.131211 1 GATATAC 225 0.0 11.8616295 1 ACTGTTC 250 0.0 11.778562 8 CTATAGT 90 7.1511968E-6 11.653102 4 GGCGAGG 270 0.0 11.582017 19 TAGGACC 225 0.0 11.441228 4 GTCCTAA 345 0.0 11.327488 1 TGTAGGA 725 0.0 11.312911 2 CTGTAGG 680 0.0 11.213726 1 GTCTAAA 95 1.3080438E-5 11.036667 1 TCCTACA 775 0.0 10.952223 2 CCTACAC 140 7.84712E-9 10.897176 3 GTAGGAC 675 0.0 10.876996 3 TATAGTG 115 8.3897066E-7 10.777967 5 ATTATAC 80 3.6393083E-4 10.726908 3 GTCCTAC 720 0.0 10.723125 1 >>END_MODULE