FastQCFastQC Report
Thu 26 May 2016
SRR1926629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679438
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC1600.017.4684961
TAGGACC1005.456968E-1217.181844
ACAGGCG400.004391041614.6483288
CTAGGAC450.004454004614.6164963
GTATTAA1059.543128E-713.9431091
TCTGTAC806.550408E-613.7029663
GTGTAGA700.001488121613.3093311
GGTCTAC700.001488121613.3093311
TAGACAG1202.419256E-913.0589045
TAGGACA1004.3659747E-712.886384
GGCGAGA1202.3646862E-1112.7873219
TCCTGTA750.00206441112.7427062
TAACACT851.5135542E-512.6337064
GGACAGT758.5787644E-512.5289346
TATGCCT600.002346465112.5284264
GTGTATA1502.5944246E-812.4220411
GTAGGAC1801.8189894E-1212.1804133
TCCTACA2202.0008883E-1111.791142
TCTGGAC758.00008E-411.6931983
TTACAGT650.00427239711.56474
Produced by FastQC (version 0.11.5)