FastQCFastQC Report
Thu 26 May 2016
SRR1926629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679438
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12970.190893061618573No Hit
GGTATCAACGCAGAGTACTTTTTTT8580.126280837986689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA801.2176133E-714.3126861
AGGCGTG551.9559693E-413.8195257
ATAATCC500.001443607313.3654443
AAAGGCG655.21302E-513.2146495
GTCTTGG1154.965841E-912.4458121
CTATTAC1001.357821E-712.4043271
TAGGACT701.04323524E-412.2707464
GTCTAGA701.04540566E-412.2680171
ATTAGAC550.002954688412.1504053
GTATAAC550.002966207512.1440971
GGCGAGA1251.4788384E-912.12001219
TGGCGAG1603.6379788E-1211.8359518
TAGTAGT657.7089545E-411.7463554
GTAGGAC2200.011.7164613
ACTGTTC1157.0296664E-811.5671958
TATGGCG1657.2759576E-1211.47812816
AGGACTG1354.3764885E-911.3113055
GTCCTAA855.079966E-511.2256361
CTTATAC1201.186927E-711.1378723
AAGGCGT600.005671707411.1345666
Produced by FastQC (version 0.11.5)