FastQCFastQC Report
Thu 26 May 2016
SRR1926628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697288
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1400.016.1381954
GTCCTAC2750.016.0400411
TGTAGGA2600.015.8220752
TCCTACA3000.015.0837122
GTCCAAT658.230951E-414.3949071
CTATAGT901.1821794E-714.3450614
GTAGGAC2700.014.2399573
TATACTC601.8157916E-413.9398755
GGACCTG1355.456968E-1213.932976
CTGTAGG2700.013.8617641
AGGACGT1600.013.7220645
TCCTATA802.0856291E-413.7124662
AGGACCT1950.013.4037265
GTGTAGC1501.7043931E-913.36671
ATAGGAC500.00882907313.1821313
TTGCACT756.477864E-512.9105564
CCTACAG3600.012.815963
GTAAGAG600.001978706312.815963
GGACGTG1553.6379788E-1212.8093426
CCCTACA750.00199806912.7983012
Produced by FastQC (version 0.11.5)