FastQCFastQC Report
Thu 26 May 2016
SRR1926628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697288
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14910.2138284324411147No Hit
GGTATCAACGCAGAGTACTTTTTTT9650.13839331811245856No Hit
CACACACACACACACACACACACAC8880.12735053521643855No Hit
TATCAACGCAGAGTACTTTTTTTTT7230.1036874290106814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGT350.00219580916.25532310
TAGGACC2550.014.5818414
AGGACCT3100.013.8400865
ATCTAGG500.001454796213.3518482
GGACCTG2850.013.045976
GTAGGAC4950.012.3280413
TCCAACG854.053125E-612.26149418
CCCGTTT701.1083925E-412.19149212
CTGTAGG5050.012.0821771
TGTAGGA5000.012.0166632
TCCTATA1353.45608E-1012.0095992
CAATAAG802.7594808E-511.9169911
GTCCTAA1800.011.6521691
GTCCTAC3700.011.5949111
CTAAATT1800.011.57946919
TTAGGAC2150.011.5306613
TAGGACG3150.011.5016734
TAGGACT1251.7293132E-811.4411374
AAAGGCG752.0025205E-411.4411375
TCCTAGG855.1063005E-511.2200412
Produced by FastQC (version 0.11.5)