##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 697288 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.00591434242379 31.0 28.0 31.0 16.0 34.0 2 28.50401268916144 31.0 28.0 33.0 22.0 34.0 3 28.87400328128406 31.0 28.0 33.0 23.0 34.0 4 32.58801384793658 35.0 32.0 37.0 26.0 37.0 5 32.21399335712073 35.0 32.0 37.0 23.0 37.0 6 32.26892188019871 35.0 32.0 37.0 24.0 37.0 7 32.07086024712888 35.0 32.0 37.0 19.0 37.0 8 32.01914273585663 35.0 32.0 37.0 19.0 37.0 9 33.19949145833572 37.0 33.0 39.0 17.0 39.0 10 33.095974690515256 37.0 33.0 39.0 17.0 39.0 11 33.32333985383371 37.0 33.0 39.0 19.0 39.0 12 33.11665050882849 37.0 33.0 39.0 17.0 39.0 13 33.25055930978304 37.0 33.0 39.0 19.0 39.0 14 33.8563721159693 38.0 33.0 40.0 17.0 41.0 15 33.98367102259038 38.0 33.0 40.0 17.0 41.0 16 33.93843720241851 38.0 33.0 40.0 17.0 41.0 17 33.85124654375237 38.0 33.0 40.0 17.0 41.0 18 33.812315714597126 38.0 33.0 40.0 17.0 41.0 19 33.87133150147428 39.0 33.0 40.0 18.0 41.0 20 33.84727831254804 39.0 33.0 40.0 16.0 41.0 21 33.76060250570783 39.0 33.0 40.0 11.0 41.0 22 33.67819466275054 39.0 33.0 40.0 10.0 41.0 23 33.33144410917727 38.0 33.0 40.0 2.0 41.0 24 33.40467353518202 39.0 33.0 40.0 2.0 41.0 25 33.18951136402749 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5733271506726325 1101 2 0.8647030412700865 1101 3 0.721975353221719 1101 4 0.6786186962581056 1101 5 0.5720984564181748 1101 6 0.38561922547776817 1101 7 0.49000660544909636 1101 8 0.5906436804250816 1101 9 0.9166303086355754 1101 10 1.0211600113034862 1101 11 0.8512846380695649 1101 12 0.7565460752824578 1101 13 0.8866404675077746 1101 14 1.1415004915843667 1101 15 1.2284973485958304 1101 16 1.0310678511148197 1101 17 0.9733769633483504 1101 18 1.0319201387487382 1101 19 0.8058636443736731 1101 20 0.8491688578856369 1101 21 0.8004951268221348 1101 22 0.8893363330164661 1101 23 1.158844924613085 1101 24 1.0177067432595308 1101 25 1.0587455697124994 1102 1 0.44427733962074 1102 2 0.4858037600412608 1102 3 0.4393550865289768 1102 4 0.34843310101918235 1102 5 0.5052129219499832 1102 6 0.20964043237070484 1102 7 0.44289810522906237 1102 8 0.4039622518237209 1102 9 0.8081203094039395 1102 10 0.9218361731073301 1102 11 0.5856263931564953 1102 12 0.5725916463527909 1102 13 0.38983267767674334 1102 14 0.7152223851168245 1102 15 0.4744770497714299 1102 16 0.5231439053474674 1102 17 0.5083781158968819 1102 18 0.944990737585016 1102 19 0.5757451065037245 1102 20 0.3693859561176964 1102 21 0.5214400769132936 1102 22 0.6678940407720688 1102 23 0.8316336030939553 1102 24 0.771629250087507 1102 25 0.8406435726602268 1103 1 0.44783929862804683 1103 2 0.23237998652517433 1103 3 0.22418694915716486 1103 4 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1107 15 0.886992444281411 1107 16 0.8730563276907191 1107 17 0.8969519684927363 1107 18 0.9672929352368413 1107 19 0.9673253358477112 1107 20 0.8804535824895225 1107 21 0.8407413714205347 1107 22 0.8346377940220222 1107 23 1.195462662455185 1107 24 1.0360070382043105 1107 25 0.9543316867301286 1108 1 1.2218517137830993 1108 2 1.0678612291454783 1108 3 0.9655415682439106 1108 4 0.7064544814903257 1108 5 -0.43990788333628217 1108 6 0.2982193155545403 1108 7 0.7032107599823334 1108 8 0.827243041636379 1108 9 1.0900377203723721 1108 10 1.4887636597565361 1108 11 1.0477544537559922 1108 12 1.1571752374653386 1108 13 0.8847598570485573 1108 14 1.3743173159999813 1108 15 1.1534606474684992 1108 16 1.1959970445839119 1108 17 1.4662529908939632 1108 18 1.4389014323474427 1108 19 1.2377989541243082 1108 20 1.4341707316200072 1108 21 1.52638532255002 1108 22 1.491271952191127 1108 23 1.5998250424004965 1108 24 1.3826428128180481 1108 25 1.41034890186544 1109 1 0.6215757856407151 1109 2 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2202 22 0.43728203143666633 2202 23 1.0340536004476135 2202 24 0.9876919965986914 2202 25 0.7230445388574012 2203 1 -0.29935716616476427 2203 2 -0.49068579151017744 2203 3 -0.5388672380349533 2203 4 -0.7226221059349385 2203 5 -0.7047663538545166 2203 6 -0.6012225001029563 2203 7 -0.3619303444859767 2203 8 -0.42040450886938885 2203 9 -0.3161788098177496 2203 10 -0.2004927083617858 2203 11 -0.5150802443427835 2203 12 -0.48506047499888894 2203 13 -0.571936938349964 2203 14 -0.2910164612362962 2203 15 -0.17349370608603465 2203 16 -0.3326531126710677 2203 17 -0.2411627856056242 2203 18 -0.48361153056304573 2203 19 -0.5703230530725847 2203 20 -0.4993089952380103 2203 21 -0.39583296436239834 2203 22 -0.5589178871537825 2203 23 -0.254381882485454 2203 24 -0.3190664496419302 2203 25 -0.34527260948242144 2204 1 0.28465621991027845 2204 2 0.3306574022296118 2204 3 0.027701971126052882 2204 4 -0.18048320032539777 2204 5 0.32299040367693976 2204 6 0.20701552552263536 2204 7 0.33470378857218464 2204 8 0.32349078163996836 2204 9 0.7204652123108346 2204 10 0.32126539637636853 2204 11 0.2804703905642043 2204 12 0.20597271788098226 2204 13 0.18550279564837524 2204 14 0.4946594352294369 2204 15 0.03577622254067592 2204 16 0.15438647227461644 2204 17 0.13171751364624384 2204 18 0.013041950676381475 2204 19 -0.0407575988919433 2204 20 0.25835718414113273 2204 21 0.02702225391374924 2204 22 0.01948237655476248 2204 23 0.17990524452460477 2204 24 0.07495066274950801 2204 25 0.1803056861577872 2205 1 0.5229885688624591 2205 2 0.5855505968745796 2205 3 0.6487405205857364 2205 4 0.2929802080396513 2205 5 0.45869213071898685 2205 6 0.3586799499877884 2205 7 0.36660867547772824 2205 8 0.586112051929156 2205 9 1.0334355277503064 2205 10 0.7342112390066333 2205 11 0.5504540115555798 2205 12 0.5775642981408566 2205 13 0.5394117458605621 2205 14 0.9706384843172415 2205 15 0.9626961759466397 2205 16 0.782342350123848 2205 17 0.9557122143943744 2205 18 0.8682770810521987 2205 19 0.8496349777804113 2205 20 0.9162252606603545 2205 21 0.9492158105922286 2205 22 0.9921199985086488 2205 23 1.1794089523602764 2205 24 1.1175374523544406 2205 25 1.1113743959622226 2206 1 0.253837231781894 2206 2 0.41034087962027854 2206 3 0.4674601807799128 2206 4 0.4515269845683534 2206 5 0.623801886297251 2206 6 0.28213315216799373 2206 7 0.5097273946056404 2206 8 0.42844885571045666 2206 9 0.5752618776055272 2206 10 0.7130186184990563 2206 11 0.5692992891914201 2206 12 0.6169900773948171 2206 13 0.39278798074388277 2206 14 0.7388769658365746 2206 15 0.3989479856894391 2206 16 0.7065779999279727 2206 17 0.3428987030675117 2206 18 0.41358186303141764 2206 19 0.4450323760089887 2206 20 0.6361223182314717 2206 21 0.54169632924323 2206 22 0.600824329116719 2206 23 0.8929426348675165 2206 24 0.7467158291216052 2206 25 0.8872963002374732 2207 1 -0.9416651309169808 2207 2 0.09333370244046435 2207 3 0.4339063997969106 2207 4 0.33833615774341297 2207 5 0.5411931848642126 2207 6 0.39271397455207335 2207 7 0.5684531593249744 2207 8 0.5371855231482883 2207 9 0.9018700950642327 2207 10 0.6941868314903132 2207 11 0.5325531390476925 2207 12 0.7060659523379798 2207 13 0.33504744948898235 2207 14 -5.0118782468595064 2207 15 -1.9476075530245183 2207 16 -0.7908558724251264 2207 17 -0.4030978718833609 2207 18 0.1841766993975682 2207 19 0.13508736825742318 2207 20 0.45471304879338703 2207 21 0.6463524548425426 2207 22 0.6317814016570296 2207 23 0.8068915995081412 2207 24 0.5625935112568357 2207 25 0.47361923875944 2208 1 0.7824265914891164 2208 2 1.0918579833313728 2208 3 1.1675781374081673 2208 4 1.0184295644915267 2208 5 1.0922258042720259 2208 6 0.9509850409863105 2208 7 1.2847100035642995 2208 8 1.2753925692270727 2208 9 1.4883974067468628 2208 10 1.5992736467784 2208 11 1.3484255027836554 2208 12 1.358937269119707 2208 13 1.3794649308089149 2208 14 1.7804869225858155 2208 15 1.6176063046562632 2208 16 1.4586724862117322 2208 17 1.3679493639270817 2208 18 1.5564303643773343 2208 19 1.2093686431353845 2208 20 1.3610036054709838 2208 21 1.6207423928188263 2208 22 1.4701722406794957 2208 23 1.8390304066970558 2208 24 1.6674278032316323 2208 25 1.5973044664655873 2209 1 -0.39898770803668526 2209 2 -0.2708171544001594 2209 3 0.107766587909218 2209 4 0.23172928979576568 2209 5 0.49876402381379137 2209 6 0.21404843585763444 2209 7 0.5376180955797025 2209 8 0.6820995962209224 2209 9 0.6252005005270718 2209 10 0.5573480141341136 2209 11 0.5475223829150124 2209 12 0.7694961752474185 2209 13 0.6121353112395269 2209 14 0.7329983170888283 2209 15 0.5680498078712759 2209 16 0.6450972978379639 2209 17 0.642622107662028 2209 18 0.6473408503893339 2209 19 0.7780349108026243 2209 20 0.5957648963876068 2209 21 0.7052343591187196 2209 22 0.7194893378051646 2209 23 0.9431961142304672 2209 24 0.758150299126541 2209 25 0.7195057156407287 2210 1 -0.24459767616186667 2210 2 -0.3482922823823884 2210 3 0.4107415710059037 2210 4 0.5995743588578719 2210 5 0.9154162019264191 2210 6 0.5957349035481627 2210 7 0.9609253495157617 2210 8 0.8366441726721945 2210 9 1.0409930244331917 2210 10 1.1303896445793953 2210 11 0.7583694803746894 2210 12 0.596850565279297 2210 13 0.8146176834987742 2210 14 1.1990371464136587 2210 15 1.036503396203159 2210 16 1.0404416424723664 2210 17 1.1174506597725227 2210 18 0.9340789939226113 2210 19 0.9813286420882363 2210 20 1.1256113177147569 2210 21 1.1098900144843142 2210 22 1.1869383863901177 2210 23 1.138395573324452 2210 24 0.9986602962288345 2210 25 1.019650601181155 2211 1 -1.6376904045827168 2211 2 -0.9163983687475685 2211 3 -0.33782791459180217 2211 4 -0.19090992734215462 2211 5 -0.02109227429644278 2211 6 -0.22191215527536912 2211 7 -0.10343565500165397 2211 8 -0.3298929624130551 2211 9 -0.0032484872003664123 2211 10 -0.02503853825443514 2211 11 -0.00369395771731007 2211 12 -0.2159390592410375 2211 13 -0.28011200028225147 2211 14 -0.12712013730907756 2211 15 -0.3897413213370342 2211 16 -0.2829663334347572 2211 17 -0.3148415634751558 2211 18 -0.12945138932895617 2211 19 -0.11649036990962713 2211 20 -0.24620099102376258 2211 21 0.01438799864907736 2211 22 0.043862658989326064 2211 23 0.15031571710451175 2211 24 0.08684701600344624 2211 25 0.0467502271587108 2212 1 0.9937068604931731 2212 2 0.1503424432939049 2212 3 0.5079537431914325 2212 4 0.5929829043295598 2212 5 0.7667028080270128 2212 6 0.6547056186676841 2212 7 0.8663929722853183 2212 8 0.7353651796254468 2212 9 0.8691236209340829 2212 10 0.501642753243047 2212 11 0.5355839727290075 2212 12 0.7975545914972031 2212 13 0.6511864438760924 2212 14 0.7818577466123244 2212 15 0.6321175521330034 2212 16 -0.015597684388659161 2212 17 0.5254247898904936 2212 18 0.3172497945983608 2212 19 0.2961001417148381 2212 20 0.5129816239045368 2212 21 0.6518285155102674 2212 22 0.6403754989400525 2212 23 1.0518474769504422 2212 24 0.9656015162025895 2212 25 0.30415912417620206 2213 1 -0.051555873453523304 2213 2 -0.12211875564685215 2213 3 -0.4922825685862371 2213 4 -1.3632421053074104 2213 5 -2.2257870309590935 2213 6 -0.9061705720178637 2213 7 -0.6174078946099151 2213 8 -0.2886836654694349 2213 9 -0.8123602914154446 2213 10 -0.5596601332935407 2213 11 -0.6998759834159216 2213 12 -1.0259134447833276 2213 13 -0.9035110966757145 2213 14 -7.676700154876279 2213 15 -3.539362364113707 2213 16 -2.368593309087874 2213 17 -1.4986923579441154 2213 18 -5.260595308919548 2213 19 -3.0186820628306705 2213 20 -5.163608828942632 2213 21 -2.26060187653961 2213 22 -1.8602435437067655 2213 23 -4.473572526017193 2213 24 -2.332041411205733 2213 25 -1.7400186168245213 2214 1 0.1957811149484847 2214 2 0.06926519746234305 2214 3 -1.7241814337289831 2214 4 -2.1463924542633137 2214 5 -0.3547838577929028 2214 6 -0.524450226341358 2214 7 -0.5967763098281793 2214 8 -0.6214328652495773 2214 9 -5.245147827846811 2214 10 -1.4394520992247273 2214 11 -0.9922682366410527 2214 12 -1.3739936099542511 2214 13 -1.4460486134768686 2214 14 -2.689279195351471 2214 15 -2.3205968042831238 2214 16 -1.3765442381743043 2214 17 -2.075553648549789 2214 18 -1.3671665804492719 2214 19 -4.007597689865861 2214 20 -2.3055579012418583 2214 21 -3.644625192613752 2214 22 -5.715586887678903 2214 23 -5.9660876412811845 2214 24 -5.7010155131812255 2214 25 -3.5493422751571977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27359.0 3 18.0 4 541.0 5 3420.0 6 4200.0 7 3282.0 8 2493.0 9 2139.0 10 1816.0 11 1708.0 12 1569.0 13 1536.0 14 1525.0 15 1574.0 16 1537.0 17 1685.0 18 1877.0 19 2199.0 20 2764.0 21 3544.0 22 4673.0 23 5885.0 24 6694.0 25 8072.0 26 9366.0 27 10073.0 28 11604.0 29 13821.0 30 16667.0 31 20978.0 32 26245.0 33 34021.0 34 43834.0 35 57435.0 36 76282.0 37 110533.0 38 174319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1501769878477 19.50296989340537 9.028676911770194 30.318176206976737 2 13.272171692537228 21.583077496915617 39.53576449660001 25.608986313947153 3 18.34210537640887 24.423290168856447 28.93468808680521 28.299916367929466 4 11.424566900216334 17.922354777439217 37.60909982957186 33.04397849277259 5 12.611170401992174 38.82974145350639 35.28706349479579 13.27202464970565 6 31.374838227651665 38.86567172773588 17.406200829483666 12.353289215128791 7 28.2026846968828 32.00578967718381 21.10702422379086 18.684501402142526 8 24.87526789911038 38.75686188264618 19.381737006803306 16.986133211440134 9 25.535318977661504 16.20518644165253 19.69065348014835 38.56884110053762 10 13.808560047850666 31.1765153069055 34.11482725135512 20.900097393888714 11 34.34895657798309 23.720551347893867 23.213289486327447 18.717202587795597 12 22.097672630896582 26.23854816225612 31.618832357142445 20.044946849704846 13 28.448790169915444 22.45729167861773 25.59659710191485 23.497321049551978 14 20.02244826779463 23.14927380427809 27.974790193379484 28.8534877345478 15 22.571915362308864 31.492030438361745 23.24137574970098 22.694678449628412 16 21.351086226974683 30.024552687888296 27.422713530940403 21.20164755419662 17 20.926060049766583 29.52850822152584 27.751970226100926 21.79346150260665 18 20.591601411238276 29.38573846197975 29.13145741904024 20.891202707741733 19 22.2317512857065 28.260302144612943 27.84311082173353 21.664835747947027 20 22.0114358158739 28.71072434004558 28.587101650262518 20.690738193818007 21 22.192551714643017 27.72957515402531 27.770160966487307 22.307712164844368 22 21.59380342125492 28.95432016612935 27.925476990856 21.52639942175973 23 21.624350340175077 28.32674590699969 28.00306329665791 22.045840456167323 24 21.61336954994077 28.199476255080413 28.783598121861043 21.403556073117773 25 21.719433003292757 28.260776035153334 28.31297828157089 21.70681267998302 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 275.0 1 275.0 2 294.5 3 314.0 4 314.0 5 314.0 6 441.5 7 569.0 8 569.0 9 569.0 10 989.5 11 1410.0 12 1410.0 13 1410.0 14 2331.5 15 3253.0 16 3253.0 17 3253.0 18 5588.0 19 7923.0 20 7923.0 21 7923.0 22 12760.0 23 17597.0 24 17597.0 25 17597.0 26 25085.5 27 32574.0 28 32574.0 29 32574.0 30 42253.5 31 51933.0 32 51933.0 33 51933.0 34 60808.0 35 69683.0 36 69683.0 37 69683.0 38 76258.5 39 82834.0 40 82834.0 41 82834.0 42 87147.0 43 91460.0 44 91460.0 45 91460.0 46 94154.0 47 96848.0 48 96848.0 49 96848.0 50 92321.0 51 87794.0 52 87794.0 53 87794.0 54 75845.5 55 63897.0 56 63897.0 57 63897.0 58 53289.0 59 42681.0 60 42681.0 61 42681.0 62 33801.5 63 24922.0 64 24922.0 65 24922.0 66 18822.5 67 12723.0 68 12723.0 69 12723.0 70 9086.0 71 5449.0 72 5449.0 73 5449.0 74 3746.0 75 2043.0 76 2043.0 77 2043.0 78 1403.0 79 763.0 80 763.0 81 763.0 82 513.0 83 263.0 84 263.0 85 263.0 86 158.0 87 53.0 88 53.0 89 53.0 90 39.0 91 25.0 92 25.0 93 25.0 94 13.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09035004187652734 2 0.0326981103934099 3 0.02681818703319145 4 0.03155080827434289 5 0.02294604238134028 6 0.3784662865272312 7 0.22472780257225136 8 0.22903018551875265 9 0.23347598123013732 10 0.01706611902112183 11 0.002151191473250651 12 0.002868255297667535 13 0.0 14 0.08246233980794163 15 0.0014341276488337674 16 0.001720953178600521 17 0.004732621241151432 18 0.004015557416734549 19 8.604765893002605E-4 20 7.170638244168837E-4 21 0.0 22 0.0 23 0.0 24 2.8682552976675346E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 697288.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.16808566708339 #Duplication Level Percentage of deduplicated Percentage of total 1 94.31873962357355 83.15902715142565 2 4.41737121424375 7.789423272815023 3 0.6084830476139028 1.6094635640697172 4 0.2093986437481413 0.7384911024222879 5 0.12134988944401083 0.5349593724095323 6 0.07406307182034023 0.3917999556613865 7 0.04657900983715511 0.2874747490727148 8 0.03132394920736606 0.2209418109717136 9 0.02421061828635484 0.192114348041195 >10 0.13069784984894134 2.135162190534335 >50 0.008646985275989495 0.5435929846950779 >100 0.007341506603831137 1.1974801770338668 >500 0.0016314459060440749 0.98560170040116 >1k 1.6314459060440747E-4 0.21446762044631196 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1491 0.2138284324411147 No Hit GGTATCAACGCAGAGTACTTTTTTT 965 0.13839331811245856 No Hit CACACACACACACACACACACACAC 888 0.12735053521643855 No Hit TATCAACGCAGAGTACTTTTTTTTT 723 0.1036874290106814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.4341276488337673E-4 0.0 8 0.0 0.0 0.0 1.4341276488337673E-4 0.0 9 0.0 0.0 0.0 1.4341276488337673E-4 0.0 10 0.0 0.0 0.0 1.4341276488337673E-4 0.0 11 0.0 0.0 0.0 1.4341276488337673E-4 0.0 12 0.0 0.0 0.0 1.4341276488337673E-4 0.0 13 0.0 0.0 0.0 1.4341276488337673E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGGT 35 0.002195809 16.255323 10 TAGGACC 255 0.0 14.581841 4 AGGACCT 310 0.0 13.840086 5 ATCTAGG 50 0.0014547962 13.351848 2 GGACCTG 285 0.0 13.04597 6 GTAGGAC 495 0.0 12.328041 3 TCCAACG 85 4.053125E-6 12.261494 18 CCCGTTT 70 1.1083925E-4 12.191492 12 CTGTAGG 505 0.0 12.082177 1 TGTAGGA 500 0.0 12.016663 2 TCCTATA 135 3.45608E-10 12.009599 2 CAATAAG 80 2.7594808E-5 11.916991 1 GTCCTAA 180 0.0 11.652169 1 GTCCTAC 370 0.0 11.594911 1 CTAAATT 180 0.0 11.579469 19 TTAGGAC 215 0.0 11.530661 3 TAGGACG 315 0.0 11.501673 4 TAGGACT 125 1.7293132E-8 11.441137 4 AAAGGCG 75 2.0025205E-4 11.441137 5 TCCTAGG 85 5.1063005E-5 11.220041 2 >>END_MODULE