FastQCFastQC Report
Thu 26 May 2016
SRR1926627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21766
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA2811.2910043186621336No Hit
GTACATGGGAAGCAGTGGTATCAAC2811.2910043186621336No Hit
CCCATGTACTCTGCGTTGATACCAC2511.15317467610034No Hit
GAGTACATGGGAAGCAGTGGTATCA1200.5513185702471746No Hit
CATGTACTCTGCGTTGATACCACTG930.4272718919415603No Hit
ACTCTGCGTTGATACCACTGCTTCC790.36295139207938987No Hit
ACGCAGAGTACATGGGAAGCAGTGG710.3261968207295783No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA690.3170081778921253No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA670.3078195350546724No Hit
CNCATGTACTCTGCGTTGATACCAC640.29403657079849305No Hit
GCGTTGATACCACTGCTTCCCATGT600.2756592851235873No Hit
GTATCAACGCAGAGTACATGGGAAG550.25268767802995495No Hit
GNACATGGGAAGCAGTGGTATCAAC520.23890471377377562No Hit
TATCAACGCAGAGTACATGGGAAGC500.22971607093632268No Hit
GGTATCAACGCAGAGTACATGGGAA470.21593310668014334No Hit
GTACTCTGCGTTGATACCACTGCTT440.20215014242396398No Hit
GTATCAACGCAGAGTACTTTTTTTT410.18836717816778462No Hit
GCAGTGGTATCAACGCAGAGTACAT350.1608012496554259No Hit
GTGGTATCAACGCAGAGTACATGGG350.1608012496554259No Hit
GNGTACATGGGAAGCAGTGGTATCA330.15161260681797298No Hit
AAACAAAAAAAAAAAAAAAAAAAAA320.14701828539924652No Hit
GATACCACTGCTTCCCATGTACTCT310.14242396398052007No Hit
CAGTGGTATCAACGCAGAGTACATG300.13782964256179364No Hit
GCTTCCCATGTACTCTGCGTTGATA290.13323532114306716No Hit
ACATGGGAAGCAGTGGTATCAACGC280.1286409997243407No Hit
GTTGATACCACTGCTTCCCATGTAC280.1286409997243407No Hit
CCACTGCTTCCCATGTACTCTGCGT260.11945235688688781No Hit
CATGGGAAGCAGTGGTATCAACGCA250.11485803546816134No Hit
TATCAACGCAGAGTACTTTTTTTTT250.11485803546816134No Hit
GGTATCAACGCAGAGTACTTTTTTT240.11026371404943491No Hit
GCAGAGTACATGGGAAGCAGTGGTA240.11026371404943491No Hit
GGGAAGCAGTGGTATCAACGCAGAG230.10566939263070844No Hit
TACCACTGCTTCCCATGTACTCTGC220.10107507121198199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1104.57622E-58.62191714
CAGTGGT950.00248443478.60215913
ATACCAC1259.673438E-58.0812519
TACATGG2407.25473E-68.0239362
GTACATG2358.198605E-67.9576231
GATACCA1301.5274351E-47.77043318
ACATGGG2558.762436E-87.7347963
CATGGGA1650.00126215767.1357624
GTGGTAT1150.0042120027.134186715
CGTTGAT1300.00262196136.687512414
Produced by FastQC (version 0.11.5)