FastQCFastQC Report
Thu 26 May 2016
SRR1926627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21766
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC7973.6616741707249836No Hit
GTACATGGGAAGCAGTGGTATCAAC7063.2435909216208767No Hit
GAGTACATGGGAAGCAGTGGTATCA3051.4012680327115685No Hit
GTATCAACGCAGAGTACTTTTTTTT3011.3828907470366627No Hit
CATGTACTCTGCGTTGATACCACTG2861.313975925755766No Hit
GGTATCAACGCAGAGTACTTTTTTT2571.1807406046126987No Hit
TATCAACGCAGAGTACTTTTTTTTT2291.052099604888358No Hit
ACGCAGAGTACATGGGAAGCAGTGG2050.941835890838923No Hit
GCGTTGATACCACTGCTTCCCATGT1840.8453551410456676No Hit
GTATCAACGCAGAGTACATGGGAAG1600.7350914269962326No Hit
ACTCTGCGTTGATACCACTGCTTCC1570.7213084627400533No Hit
TATCAACGCAGAGTACATGGGAAGC1480.6799595699715153No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1420.6523936414591566No Hit
GGTATCAACGCAGAGTACATGGGAA1210.5559128916659009No Hit
GCTTCCCATGTACTCTGCGTTGATA980.4502434990351925No Hit
GGGAAGCAGTGGTATCAACGCAGAG910.4180832491041073No Hit
GTGGTATCAACGCAGAGTACATGGG870.39970596342920156No Hit
CCACTGCTTCCCATGTACTCTGCGT810.3721400349168428No Hit
GTACTCTGCGTTGATACCACTGCTT750.34457410640448405No Hit
ATACCACTGCTTCCCATGTACTCTG750.34457410640448405No Hit
CATGGGAAGCAGTGGTATCAACGCA710.3261968207295783No Hit
CTGCTTCCCATGTACTCTGCGTTGA680.3124138564733989No Hit
GAGTACTTTTTTTTTTTTTTTTTTT660.30322521363594596No Hit
ACATGGGAAGCAGTGGTATCAACGC660.30322521363594596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA660.30322521363594596No Hit
CAGTGGTATCAACGCAGAGTACATG650.2986308922172195No Hit
AAGCAGTGGTATCAACGCAGAGTAC650.2986308922172195No Hit
GTACTTTTTTTTTTTTTTTTTTTTT580.2664706422861343No Hit
GCAGTGGTATCAACGCAGAGTACAT570.2618763208674079No Hit
GCAGAGTACATGGGAAGCAGTGGTA550.25268767802995495No Hit
GTTGATACCACTGCTTCCCATGTAC540.24809335661122853No Hit
GCAGAGTACTTTTTTTTTTTTTTTT510.23431039235504916No Hit
TACCACTGCTTCCCATGTACTCTGC490.22512174951759625No Hit
GATACCACTGCTTCCCATGTACTCT490.22512174951759625No Hit
CCATGTACTCTGCGTTGATACCACT470.21593310668014334No Hit
CTTCCCATGTACTCTGCGTTGATAC440.20215014242396398No Hit
ATGGGAAGCAGTGGTATCAACGCAG410.18836717816778462No Hit
ATCAACGCAGAGTACTTTTTTTTTT400.18377285674905816No Hit
AAAAAGTACTCTGCGTTGATACCAC380.17458421391160525No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA370.16998989249287882No Hit
GGAAGCAGTGGTATCAACGCAGAGT270.12404667830561426No Hit
GTGGTATCAACGCAGAGTACTTTTT270.12404667830561426No Hit
ATCAACGCAGAGTACATGGGAAGCA260.11945235688688781No Hit
CTCTGCGTTGATACCACTGCTTCCC240.11026371404943491No Hit
CTGCGTTGATACCACTGCTTCCCAT230.10566939263070844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA1751.2732926E-1110.8835362
GAAGCAG1656.4210326E-1010.3888319
GGAAGCA1656.4210326E-1010.3888318
CCCATGT1854.3291948E-109.7804761
TGTACTC1951.05501385E-109.7672775
GTACTCT2052.5465852E-119.7553656
TACTCTG2052.5465852E-119.7553657
CAGTGGT1951.1459633E-109.72236913
ATGTACT2001.70985E-109.5230944
TGCGTTG2104.5474735E-119.4793112
AGTGGTA2001.8553692E-109.4793114
AGCAGTG2001.8553692E-109.4793111
GCAGTGG2001.8553692E-109.4793112
AAGCAGT2001.8553692E-109.4793110
CATGTAC2052.728484E-109.2908243
GGGAAGC1951.1277734E-99.2789137
CTGCGTT2157.2759576E-119.25886111
CTCTGCG2201.03682396E-109.0902279
TCTGCGT2201.1459633E-109.04843210
TTGATAC2201.1459633E-109.04843216
Produced by FastQC (version 0.11.5)