##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21766 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.790039511164203 30.0 26.0 31.0 10.0 33.0 2 27.117936230818707 31.0 26.0 31.0 15.0 34.0 3 27.535881650280253 31.0 27.0 31.0 16.0 34.0 4 31.18896443995222 35.0 31.0 37.0 19.0 37.0 5 30.823715887163466 35.0 31.0 37.0 10.0 37.0 6 30.742626114122945 35.0 32.0 37.0 8.0 37.0 7 30.424147753376825 35.0 31.0 37.0 7.0 37.0 8 30.382201598823855 35.0 31.0 37.0 2.0 37.0 9 31.399476247358265 37.0 31.0 39.0 2.0 39.0 10 31.191353487089955 37.0 30.0 39.0 2.0 39.0 11 31.445005972617846 37.0 31.0 39.0 2.0 39.0 12 31.216070936322705 37.0 31.0 39.0 2.0 39.0 13 31.333088302857668 37.0 31.0 39.0 2.0 39.0 14 31.79711476614904 38.0 31.0 40.0 2.0 41.0 15 31.87016447670679 38.0 31.0 40.0 2.0 41.0 16 31.676835431406783 38.0 31.0 40.0 2.0 41.0 17 31.6316732518607 38.0 31.0 40.0 2.0 41.0 18 31.646466966829 38.0 31.0 40.0 2.0 41.0 19 31.59827253514656 38.0 31.0 40.0 2.0 41.0 20 31.63608380042268 38.0 31.0 40.0 2.0 41.0 21 31.524901222089497 38.0 31.0 40.0 2.0 41.0 22 31.472847560415328 38.0 31.0 40.0 2.0 41.0 23 31.080400624827714 38.0 30.0 40.0 2.0 41.0 24 31.059358632729946 38.0 30.0 40.0 2.0 41.0 25 30.843195809978866 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9603070032765899 1101 2 0.4683634146266442 1101 3 0.8999405589085718 1101 4 1.5056809388577612 1101 5 1.4819962855987292 1101 6 2.3635176524422583 1101 7 0.5789923709900755 1101 8 0.8006981369426249 1101 9 1.4109706716093413 1101 10 0.928310861945846 1101 11 1.0614798642654435 1101 12 2.278825384807753 1101 13 1.8212718185495902 1101 14 3.053878419883439 1101 15 1.9320598289765805 1101 16 1.331285367764636 1101 17 0.07301667548559365 1101 18 1.445893679645259 1101 19 1.6562152948901883 1101 20 1.4586404346927218 1101 21 1.6682225306679008 1101 22 2.411592732965399 1101 23 3.2329639658291036 1101 24 2.104977816417801 1101 25 3.014805232055405 1102 1 3.099195892165479 1102 2 2.412807859071087 1102 3 2.6221627811307933 1102 4 1.0056809388577612 1102 5 0.7875518411542863 1102 6 0.446850985775594 1102 7 2.273436815434522 1102 8 2.5506981369426214 1102 9 3.2720817827204556 1102 10 2.7894219730569603 1102 11 2.478146530932108 1102 12 2.5843809403633102 1102 13 2.82127181854959 1102 14 4.248322864327882 1102 15 2.848726495643252 1102 16 3.4979520344313073 1102 17 3.9063500088189294 1102 18 3.445893679645259 1102 19 3.4617708504457454 1102 20 4.264195990248279 1102 21 3.4460003084456794 1102 22 3.6338149551876207 1102 23 2.8162972991624393 1102 24 2.8827555941955723 1102 25 3.070360787610962 1103 1 1.216717259686849 1103 2 1.7974232436864739 1103 3 1.5965217554897677 1103 4 1.648843332020153 1103 5 2.099517653120099 1103 6 1.0408680797926877 1103 7 2.202923994921697 1103 8 1.4566810429255312 1103 9 2.9323381929768644 1103 10 3.8407040243390114 1103 11 3.2302832830688573 1103 12 1.921987777970152 1103 13 3.620417117694892 1103 14 3.761143377148393 1103 15 3.0602649571817118 1103 16 2.8248751113543804 1103 17 2.3016491541180706 1103 18 2.809141542893123 1103 19 3.7673264060013025 1103 20 4.225734451786739 1103 21 3.614803727249093 1103 22 4.392361963734633 1103 23 4.324844307709444 1103 24 3.5836102950502777 1103 25 4.997711214961388 1104 1 0.5480263015222064 1104 2 1.55315873626407 1104 3 0.9642680442886871 1104 4 0.6387218745302761 1104 5 1.2992477475870352 1104 6 1.5111784711557128 1104 7 1.1637876926275013 1104 8 0.8723355638432082 1104 9 1.285239677457298 1104 10 1.4736324993727479 1104 11 0.7559243087098864 1104 12 -0.5442740303969238 1104 13 0.06980983024549658 1104 14 0.827270232748937 1104 15 0.8969721096783339 1104 16 0.4935660695190265 1104 17 -0.35826987422200673 1104 18 0.31723870888502503 1104 19 0.24978254635217567 1104 20 1.5624416042833609 1104 21 0.6404447528901223 1104 22 1.6528208031408376 1104 23 0.18179437518583086 1104 24 0.4149193368856352 1104 25 0.546968974745468 1105 1 2.932529225498815 1105 2 2.412807859071087 1105 3 1.1777183366863504 1105 4 1.9779031610799827 1105 5 2.620885174487622 1105 6 2.2246287635533726 1105 7 3.773436815434522 1105 8 2.467364803609293 1105 9 3.88319289383157 1105 10 3.8449775286125174 1105 11 4.228146530932108 1105 12 3.223269829252203 1105 13 4.460160707438476 1105 14 3.692767308772325 1105 15 2.7931709400876947 1105 16 2.831285367764636 1105 17 3.545238897707815 1105 18 3.6681159018674805 1105 19 3.3228819615568526 1105 20 3.3475293235816075 1105 21 3.3626669751123437 1105 22 2.5227038440765135 1105 23 3.3718528547179965 1105 24 4.2438667053066865 1105 25 4.709249676499855 1106 1 2.4047514477210363 1106 2 1.635030081293312 1106 3 2.7054961144641254 1106 4 3.03345871663554 1106 5 3.1486629522653935 1106 6 2.641295430220037 1106 7 3.0512145932123005 1106 8 2.77292035916485 1106 9 3.5776373382760127 1106 10 4.011644195279182 1106 11 3.228146530932108 1106 12 3.778825384807753 1106 13 3.1823829296606974 1106 14 4.664989530994546 1106 15 4.043170940087695 1106 16 3.525729812209086 1106 17 3.0174611199300365 1106 18 4.307004790756373 1106 19 3.850659739334631 1106 20 4.069751545803829 1106 21 3.4460003084456794 1106 22 4.161592732965399 1106 23 4.816297299162439 1106 24 4.632755594195572 1106 25 4.959249676499855 1107 1 2.0019736699432578 1107 2 1.9044745257377542 1107 3 2.147162781130792 1107 4 2.78345871663554 1107 5 2.3847740633765078 1107 6 1.9690732079978126 1107 7 2.190103482101186 1107 8 2.211809248053733 1107 9 0.5470817827204542 1107 10 0.11442197305695956 1107 11 0.4309243087098835 1107 12 -0.1350635040811312 1107 13 0.5934940407718088 1107 14 0.6483228643278807 1107 15 1.5153931623099162 1107 16 0.8396187010979688 1107 17 0.27857223104114937 1107 18 0.5014492352008162 1107 19 0.2589930726679661 1107 20 -0.2191373430850554 1107 21 0.8404447528901251 1107 22 0.3949260662987335 1107 23 1.335741743606885 1107 24 1.143866705306685 1107 25 1.3509163431665208 1108 1 0.26586255883214704 1108 2 -0.08719214092891292 1108 3 -0.18339277442476387 1108 4 -0.8832079500311281 1108 5 -2.4902259366234922 1108 6 -0.5809267920021846 1108 7 0.5512145932122969 1108 8 0.7729203591648464 1108 9 0.5220817827204556 1108 10 -0.5439113602763719 1108 11 -0.04963124684567077 1108 12 0.9454920514744245 1108 13 -0.4565059592281884 1108 14 0.4149895309945464 1108 15 1.404282051198802 1108 16 0.33128536776463946 1108 17 -0.28809443562551706 1108 18 0.3625603463119269 1108 19 -0.8160069273320332 1108 20 0.4864182124705003 1108 21 -0.35955524710987774 1108 22 -0.6161850448123758 1108 23 -0.29481381194867495 1108 24 0.3827555941955758 1108 25 0.48702745427763006 1109 1 -0.481134438164851 1109 2 -0.677282231019003 1109 3 -0.13459397562596465 1109 4 0.8172425004193222 1109 5 -1.219955666353222 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0.6094339754389964 2201 15 1.5431709400876947 2201 16 1.1646187010979716 2201 17 1.1285722310411508 2201 18 0.6681159018674805 2201 19 -1.482673593998701 2201 20 0.45864043469272175 2201 21 0.4460003084456794 2201 22 0.5782593996320706 2201 23 1.8718528547179965 2201 24 -0.4783555169155349 2201 25 -0.12408365683348066 2202 1 0.9412593842289709 2202 2 0.6509030971663279 2202 3 1.1078770668450773 2202 4 -0.3665412833644588 2202 5 1.6812026348050786 2202 6 1.4262160651406752 2202 7 0.5186749106726154 2202 8 2.0618092480537342 2202 9 0.06493892557760006 2202 10 1.7322791159141033 2202 11 1.7130671658527419 2202 12 2.4970793530617215 2202 13 1.7720654693432394 2202 14 -0.18024856424354496 2202 15 2.1225360194527703 2202 16 1.5860472725265424 2202 17 3.178572231041148 2202 18 2.472877806629384 2202 19 0.49113592981082377 2202 20 2.2022912283435154 2202 21 2.1547304671758347 2202 22 1.9520689234415904 2202 23 0.1321703150354523 2202 24 1.1938667053066894 2202 25 -0.6740836568334814 2203 1 -1.697350654381065 2203 2 -0.893498447235217 2203 3 -2.1075669485989366 2203 4 -1.6151899320131093 2203 5 -1.1659016122991694 2203 6 -0.10119706227245473 2203 7 -0.6612478692501647 2203 8 -0.44629886005437314 2203 9 -0.09953983890116547 2203 10 -1.4538212701862818 2203 11 -1.257589204803626 2203 12 -0.33303647705410455 2203 13 -0.8078573105795392 2203 14 0.221295837300854 2203 15 -0.7616338647171119 2203 16 -0.9367326502533793 2203 17 -1.1619683094993896 2203 18 -0.8228750891235066 2203 19 -0.5660069273320332 2203 20 -0.3258940998418147 2203 21 -0.5757714633260953 2203 22 -0.6672360958634265 2203 23 -0.509528526663388 2203 24 -0.4926197811798012 2203 25 0.9097001269503018 2204 1 0.9047514477210363 2204 2 1.4683634146266442 2204 3 1.5943850033530147 2204 4 2.394569827746654 2204 5 3.0931073967098435 2204 6 2.6690732079978154 2204 7 2.440103482101186 2204 8 2.02292035916485 2204 9 3.6609706716093413 2204 10 3.733866417501403 2204 11 3.728146530932108 2204 12 1.667714273696646 2204 13 2.9879384852162545 2204 14 3.331656197661218 2204 15 3.959837606754359 2204 16 5.136840923320193 2204 17 3.934127786596708 2204 18 3.8070047907563733 2204 19 4.87843751711241 2204 20 3.9030848791371646 2204 21 4.168222530667901 2204 22 4.856037177409849 2204 23 5.399630632495768 2204 24 5.299422260862244 2204 25 5.4314718987220765 2205 1 0.9300039729735623 2205 2 1.3522017984650283 2205 3 1.591859750827762 2205 4 1.0940647772416021 2205 5 3.327955881558328 2205 6 2.502406541331151 2205 7 1.4173762093739128 2205 8 2.540597126841611 2205 9 3.037233297871971 2205 10 3.0015431851781713 2205 11 3.5286515814371597 2205 12 3.185391041373414 2205 13 3.9677364650142337 2205 14 3.066504682509702 2205 15 4.35630225321901 2205 16 3.8085580950373625 2205 17 3.439178291647213 2205 18 3.7135704473220272 2205 19 3.646114284789178 2205 20 2.1278323538846386 2205 21 3.428323540768911 2205 22 3.2525018238744927 2205 23 2.51831750118264 2205 24 2.5696242810642644 2205 25 2.216825434075613 2206 1 3.3374999857327303 2206 2 3.60579031521144 2206 3 3.490583833762372 2206 4 4.13872187453028 2206 5 4.851879326534405 2206 6 4.958546892208343 2206 7 5.242735061048556 2206 8 5.451282932264263 2206 9 5.679976519562558 2206 10 5.921000920425378 2206 11 5.282240098183571 2206 12 4.666252285392552 2206 13 5.517178251298127 2206 14 4.5377965485384095 2206 15 4.870656320204656 2206 16 5.019881858992711 2206 17 5.115414336304308 2206 18 5.948817656253446 2206 19 6.039256230562707 2206 20 5.746652130599156 2206 21 6.008865805521705 2206 22 5.863347118930317 2206 23 6.234425954133197 2206 24 6.0201824947803715 2206 25 6.704863711587574 2207 1 0.6841165270861147 2207 2 1.2223316685948973 2207 3 1.479305638273651 2207 4 0.9191730023498259 2207 5 0.42405977766222236 2207 6 0.2833589222835293 2207 7 0.43296062495833 2207 8 0.7189521051965926 2207 9 1.5220817827204556 2207 10 1.3037076873426727 2207 11 1.2844957372813184 2207 12 -0.045777789795419466 2207 13 1.0292083264860992 2207 14 -5.323105707100691 2207 15 -1.8203211234043692 2207 16 0.21461870109797232 2207 17 0.5785722310411536 2207 18 1.3300206637722454 2207 19 0.6054216440965376 2207 20 1.8022912283435168 2207 21 1.4118733243186945 2207 22 2.180640352013018 2207 23 1.2750274578925946 2207 24 2.2224381338781143 2207 25 0.8116306288808062 2208 1 0.37056341353300226 2208 2 0.7974232436864739 2208 3 -0.22399106502305344 2208 4 0.1873048704816931 2208 5 -1.6440720904696455 2208 6 -0.1642601253355167 2208 7 -0.899640107642405 2208 8 -0.9792163929719067 2208 9 -0.5804823198436466 2208 10 -1.5182703346353463 2208 11 -1.6415115887260114 2208 12 0.10147495745732726 2208 13 -0.3283008310230606 2208 14 0.6072972233022398 2208 15 -1.2987094017926495 2208 16 -1.5084582219789517 2208 17 0.30164915411807414 2208 18 -0.08829435454277501 2208 19 0.43399307266796683 2208 20 0.09752932358161104 2208 21 -0.744170631725261 2208 22 -1.0435354721628052 2208 23 -0.03413005126491342 2208 24 -0.4163897049497223 2208 25 -0.5663913491411741 2209 1 -2.3730263300567422 2209 2 -1.920525474262245 2209 3 -1.4318912729232594 2209 4 -1.1827574995806778 2209 5 -1.1118475582451133 2209 6 -0.047143008218402116 2209 7 -1.0666532746555717 2209 8 -1.5003529141084258 2209 9 -1.9914317307930567 2209 10 -1.9943618107268222 2209 11 -0.7981297453441663 2209 12 -0.7384418824595116 2209 13 -1.294343797066027 2209 14 -0.40032578432076704 2209 15 -0.3562284593117049 2209 16 -0.23402994755067752 2209 17 0.16235601482493323 2209 18 -0.8769291431775628 2209 19 0.08264172131661596 2209 20 -0.21778599173370594 2209 21 -0.8190147065693374 2209 22 -0.45101987964721246 2209 23 0.9769579598230997 2209 24 -1.6007278892879064 2209 25 -1.036245818995642 2210 1 -0.07302633005674153 2210 2 -0.15385880759557935 2210 3 0.35549611446412754 2210 4 1.2538330220479565E-4 2210 5 1.876440730043175 2210 6 1.2024065413311469 2210 7 2.0567701487678534 2210 8 1.361809248053735 2210 9 2.122081782720457 2210 10 2.2227553063902903 2210 11 2.5559243087098835 2210 12 1.8066031625855317 2210 13 1.8434940407718088 2210 14 2.9816561976612164 2210 15 2.7987264956432476 2210 16 1.3812853677646402 2210 17 0.7452388977078179 2210 18 2.634782568534149 2210 19 2.2673264060013025 2210 20 2.264195990248279 2210 21 1.407111419556788 2210 22 1.2615927329654006 2210 23 2.4274084102735465 2210 24 2.6272000386400194 2210 25 1.0925830098331843 2211 1 1.6269736699432578 2211 2 2.0206509963259904 2211 3 3.7398098399543223 2211 4 3.445223422517895 2211 5 4.333303475141211 2211 6 4.0514261491742865 2211 7 3.4842211291600123 2211 8 4.161809248053736 2211 9 4.198552370955753 2211 10 4.730598443645192 2211 11 4.197100779298118 2211 12 4.934054142977693 2211 13 5.337611687830638 2211 14 4.454205217269056 2211 15 4.47127551525109 2211 16 4.64991281874503 2211 17 3.672689878099977 2211 18 4.089684529318461 2211 19 3.169287190315025 2211 20 3.254392068679646 2211 21 2.8463271058312962 2211 22 2.524337831004619 2211 23 2.613682920077469 2211 24 3.3468078817772735 2211 25 4.478857519637106 2212 1 1.1269736699432578 2212 2 1.108886290443639 2212 3 1.3574568987778513 2212 4 2.209929304870837 2212 5 2.3627152398470948 2212 6 2.4043673256448734 2212 7 3.160691717395302 2212 8 3.1618092480537356 2212 9 3.6985523709557526 2212 10 2.0541278554099023 2212 11 2.1088654851804733 2212 12 3.6399364959188674 2212 13 3.1317293348894566 2212 14 4.013028746680824 2212 15 3.353628456427561 2212 16 3.3263834069803266 2212 17 2.055042819276448 2212 18 2.324978646965519 2212 19 2.610463660903264 2212 20 2.78380383338553 2212 21 2.963974164654825 2212 22 3.553749595710503 2212 23 2.201918214195114 2212 24 2.4056314111890416 2212 25 2.5376810490488744 2213 1 1.1582236699432578 2213 2 0.42322452573775493 2213 3 0.24716278113079326 2213 4 -0.9352912833644602 2213 5 0.10352406337650777 2213 6 0.13782320799781544 2213 7 -1.6223965178988138 2213 8 -1.4319407519462644 2213 9 -0.8216682172795444 2213 10 -0.42932802694303973 2213 11 -0.9940756912901136 2213 12 -1.9538135040811326 2213 13 -1.5502559592281884 2213 14 -7.376677135672118 2213 15 -3.515856837690084 2213 16 -4.054131298902028 2213 17 -1.2776777689588492 2213 18 -7.404800764799184 2213 19 -2.316006927332033 2213 20 -3.944137343085057 2213 21 -1.4533052471098777 2213 22 -2.723823933701265 2213 23 -5.364258256393118 2213 24 -3.0686332946933135 2213 25 -0.5615836568334807 2214 1 -3.603795560825972 2214 2 -3.330781884518654 2214 3 -5.685529526561513 2214 4 -5.42807974490292 2214 5 -5.233815680213237 2214 6 -6.164260125335517 2214 7 -5.361178569180865 2214 8 -5.235626649382162 2214 9 -8.580482319843647 2214 10 -6.1080139243789375 2214 11 -5.128691075905497 2214 12 -5.283140427158056 2214 13 -5.610352113074342 2214 14 -8.315779699774684 2214 15 -9.170504273587518 2214 16 -7.893073606594335 2214 17 -8.493222640753721 2214 18 -6.80624307249149 2214 19 -8.078827440152548 2214 20 -7.030675804623517 2214 21 -7.9749398624944945 2214 22 -9.325586754214086 2214 23 -10.085412102546965 2214 24 -10.801005089565109 2214 25 -9.848442631192455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1462.0 3 1.0 4 32.0 5 210.0 6 225.0 7 184.0 8 126.0 9 100.0 10 93.0 11 76.0 12 65.0 13 63.0 14 59.0 15 70.0 16 75.0 17 66.0 18 79.0 19 65.0 20 115.0 21 171.0 22 186.0 23 199.0 24 226.0 25 272.0 26 299.0 27 308.0 28 374.0 29 457.0 30 564.0 31 675.0 32 839.0 33 1061.0 34 1333.0 35 1670.0 36 2283.0 37 3263.0 38 4420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.58542193607726 17.567256840653023 8.388135203495056 27.459186019774663 2 14.432752837384552 22.34066994440105 33.262877360658 29.963699857556403 3 21.377694039795966 28.656771288084187 26.276365975828313 23.68916869629153 4 13.489911292917222 19.48338465781128 29.489359746288553 37.53734430298295 5 12.770552823859196 38.91365286521759 32.604200174624324 15.711594136298885 6 33.33487063598211 32.306415163953325 17.04561176958908 17.31310243047549 7 25.831107836817385 33.47914172575744 20.158393958928077 20.5313564784971 8 29.78400036844287 32.10979597476166 18.9655966471699 19.140607009625572 9 28.779993552249806 14.221894717450375 18.32082162759637 38.67729010270345 10 18.461679838265024 29.41095386877412 28.487410402499542 23.639955890461312 11 31.60119457845164 25.73397656788422 17.43165632896853 25.23317252469561 12 25.389386629910405 29.13852515506547 26.124511830921204 19.34757638410292 13 32.55536157309565 20.467701920426354 21.76330056050721 25.21363594597078 14 23.1395081590439 24.638014249597795 24.435761893817514 27.786715697540792 15 33.19397225029863 22.911880915188824 21.501424239639803 22.392722594872737 16 20.904162455205366 26.44491408618947 29.59661857943582 23.054304879169347 17 24.843778717147583 27.467377320345527 26.66329718801691 21.025546774489985 18 25.87181254307374 22.412129565816677 29.14311968757179 22.57293820353779 19 23.325369842874206 27.455664798309286 30.510888541762384 18.70807681705412 20 24.15234769824497 23.784801984746853 29.417440044105486 22.64541027290269 21 21.533584489570888 25.755765873380504 30.970320683635027 21.74032895341358 22 23.56886887806671 24.37287512634384 24.896627768078655 27.161628227510796 23 20.481484884682533 25.273362124414223 29.265827437287513 24.97932555361573 24 22.48920334466599 29.79876872185978 26.899751906643388 20.812276026830837 25 22.861343379582834 22.54433520169071 25.696039694937056 28.8982817237894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 86.0 1 86.0 2 83.0 3 80.0 4 80.0 5 80.0 6 107.0 7 134.0 8 134.0 9 134.0 10 151.0 11 168.0 12 168.0 13 168.0 14 147.5 15 127.0 16 127.0 17 127.0 18 178.5 19 230.0 20 230.0 21 230.0 22 348.5 23 467.0 24 467.0 25 467.0 26 606.0 27 745.0 28 745.0 29 745.0 30 866.0 31 987.0 32 987.0 33 987.0 34 1075.5 35 1164.0 36 1164.0 37 1164.0 38 1112.0 39 1060.0 40 1060.0 41 1060.0 42 1259.5 43 1459.0 44 1459.0 45 1459.0 46 2859.0 47 4259.0 48 4259.0 49 4259.0 50 3940.5 51 3622.0 52 3622.0 53 3622.0 54 2877.0 55 2132.0 56 2132.0 57 2132.0 58 1862.5 59 1593.0 60 1593.0 61 1593.0 62 1484.0 63 1375.0 64 1375.0 65 1375.0 66 1185.5 67 996.0 68 996.0 69 996.0 70 771.5 71 547.0 72 547.0 73 547.0 74 442.5 75 338.0 76 338.0 77 338.0 78 239.0 79 140.0 80 140.0 81 140.0 82 95.0 83 50.0 84 50.0 85 50.0 86 28.0 87 6.0 88 6.0 89 6.0 90 3.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09648074979325554 2 0.013782964256179364 3 0.02297160709363227 4 0.041348892768538086 5 0.02297160709363227 6 0.3813286777542957 7 0.22052742809886983 8 0.24349903519250204 9 0.24349903519250204 10 0.009188642837452908 11 0.004594321418726454 12 0.004594321418726454 13 0.0 14 0.050537535605990995 15 0.0 16 0.0 17 0.009188642837452908 18 0.004594321418726454 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 21766.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.04337039419278 #Duplication Level Percentage of deduplicated Percentage of total 1 93.00869565217391 61.42607736837269 2 4.883478260869565 6.450427271891941 3 0.9391304347826087 1.860700174584214 4 0.2782608695652174 0.7350914269962326 5 0.1391304347826087 0.45943214187264536 6 0.0765217391304348 0.30322521363594596 7 0.09739130434782609 0.4502434990351925 8 0.0765217391304348 0.40430028484792796 9 0.034782608695652174 0.20674446384269046 >10 0.2434782608695652 3.5973536708628138 >50 0.12521739130434784 5.738307451989341 >100 0.08347826086956522 11.462831939722502 >500 0.013913043478260868 6.905265092345861 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 797 3.6616741707249836 No Hit GTACATGGGAAGCAGTGGTATCAAC 706 3.2435909216208767 No Hit GAGTACATGGGAAGCAGTGGTATCA 305 1.4012680327115685 No Hit GTATCAACGCAGAGTACTTTTTTTT 301 1.3828907470366627 No Hit CATGTACTCTGCGTTGATACCACTG 286 1.313975925755766 No Hit GGTATCAACGCAGAGTACTTTTTTT 257 1.1807406046126987 No Hit TATCAACGCAGAGTACTTTTTTTTT 229 1.052099604888358 No Hit ACGCAGAGTACATGGGAAGCAGTGG 205 0.941835890838923 No Hit GCGTTGATACCACTGCTTCCCATGT 184 0.8453551410456676 No Hit GTATCAACGCAGAGTACATGGGAAG 160 0.7350914269962326 No Hit ACTCTGCGTTGATACCACTGCTTCC 157 0.7213084627400533 No Hit TATCAACGCAGAGTACATGGGAAGC 148 0.6799595699715153 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 142 0.6523936414591566 No Hit GGTATCAACGCAGAGTACATGGGAA 121 0.5559128916659009 No Hit GCTTCCCATGTACTCTGCGTTGATA 98 0.4502434990351925 No Hit GGGAAGCAGTGGTATCAACGCAGAG 91 0.4180832491041073 No Hit GTGGTATCAACGCAGAGTACATGGG 87 0.39970596342920156 No Hit CCACTGCTTCCCATGTACTCTGCGT 81 0.3721400349168428 No Hit GTACTCTGCGTTGATACCACTGCTT 75 0.34457410640448405 No Hit ATACCACTGCTTCCCATGTACTCTG 75 0.34457410640448405 No Hit CATGGGAAGCAGTGGTATCAACGCA 71 0.3261968207295783 No Hit CTGCTTCCCATGTACTCTGCGTTGA 68 0.3124138564733989 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 66 0.30322521363594596 No Hit ACATGGGAAGCAGTGGTATCAACGC 66 0.30322521363594596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 66 0.30322521363594596 No Hit CAGTGGTATCAACGCAGAGTACATG 65 0.2986308922172195 No Hit AAGCAGTGGTATCAACGCAGAGTAC 65 0.2986308922172195 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 58 0.2664706422861343 No Hit GCAGTGGTATCAACGCAGAGTACAT 57 0.2618763208674079 No Hit GCAGAGTACATGGGAAGCAGTGGTA 55 0.25268767802995495 No Hit GTTGATACCACTGCTTCCCATGTAC 54 0.24809335661122853 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 51 0.23431039235504916 No Hit TACCACTGCTTCCCATGTACTCTGC 49 0.22512174951759625 No Hit GATACCACTGCTTCCCATGTACTCT 49 0.22512174951759625 No Hit CCATGTACTCTGCGTTGATACCACT 47 0.21593310668014334 No Hit CTTCCCATGTACTCTGCGTTGATAC 44 0.20215014242396398 No Hit ATGGGAAGCAGTGGTATCAACGCAG 41 0.18836717816778462 No Hit ATCAACGCAGAGTACTTTTTTTTTT 40 0.18377285674905816 No Hit AAAAAGTACTCTGCGTTGATACCAC 38 0.17458421391160525 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 37 0.16998989249287882 No Hit GGAAGCAGTGGTATCAACGCAGAGT 27 0.12404667830561426 No Hit GTGGTATCAACGCAGAGTACTTTTT 27 0.12404667830561426 No Hit ATCAACGCAGAGTACATGGGAAGCA 26 0.11945235688688781 No Hit CTCTGCGTTGATACCACTGCTTCCC 24 0.11026371404943491 No Hit CTGCGTTGATACCACTGCTTCCCAT 23 0.10566939263070844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGTA 175 1.2732926E-11 10.883536 2 GAAGCAG 165 6.4210326E-10 10.388831 9 GGAAGCA 165 6.4210326E-10 10.388831 8 CCCATGT 185 4.3291948E-10 9.780476 1 TGTACTC 195 1.05501385E-10 9.767277 5 GTACTCT 205 2.5465852E-11 9.755365 6 TACTCTG 205 2.5465852E-11 9.755365 7 CAGTGGT 195 1.1459633E-10 9.722369 13 ATGTACT 200 1.70985E-10 9.523094 4 TGCGTTG 210 4.5474735E-11 9.47931 12 AGTGGTA 200 1.8553692E-10 9.47931 14 AGCAGTG 200 1.8553692E-10 9.47931 11 GCAGTGG 200 1.8553692E-10 9.47931 12 AAGCAGT 200 1.8553692E-10 9.47931 10 CATGTAC 205 2.728484E-10 9.290824 3 GGGAAGC 195 1.1277734E-9 9.278913 7 CTGCGTT 215 7.2759576E-11 9.258861 11 CTCTGCG 220 1.03682396E-10 9.090227 9 TCTGCGT 220 1.1459633E-10 9.048432 10 TTGATAC 220 1.1459633E-10 9.048432 16 >>END_MODULE