FastQCFastQC Report
Thu 26 May 2016
SRR1926626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27399
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA4631.6898426949888683No Hit
CCCATGTACTCTGCGTTGATACCAC4051.4781561370852951No Hit
GTACATGGGAAGCAGTGGTATCAAC3741.365013321654075No Hit
GAGTACATGGGAAGCAGTGGTATCA1960.7153545749844885No Hit
CATGTACTCTGCGTTGATACCACTG1500.5474652359575167No Hit
ACGCAGAGTACATGGGAAGCAGTGG1500.5474652359575167No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1400.5109675535603488No Hit
GCGTTGATACCACTGCTTCCCATGT1370.5000182488411986No Hit
ACTCTGCGTTGATACCACTGCTTCC1040.3795758969305449No Hit
GGTATCAACGCAGAGTACATGGGAA1010.3686265922113946No Hit
GTATCAACGCAGAGTACATGGGAAG990.361327055731961No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA960.3503777510128107No Hit
TATCAACGCAGAGTACATGGGAAGC900.32847914157451No Hit
CNCATGTACTCTGCGTTGATACCAC900.32847914157451No Hit
GCTTCCCATGTACTCTGCGTTGATA660.24088470382130733No Hit
GNACATGGGAAGCAGTGGTATCAAC610.22263586262272345No Hit
CATGGGAAGCAGTGGTATCAACGCA600.21898609438300667No Hit
AAACAAAAAAAAAAAAAAAAAAAAA560.20438702142413956No Hit
CAGTGGTATCAACGCAGAGTACATG560.20438702142413956No Hit
GTGGTATCAACGCAGAGTACATGGG510.18613818022555567No Hit
ATACCACTGCTTCCCATGTACTCTG490.17883864374612213No Hit
GTACTCTGCGTTGATACCACTGCTT480.17518887550640536No Hit
CTGCTTCCCATGTACTCTGCGTTGA480.17518887550640536No Hit
GCAGAGTACATGGGAAGCAGTGGTA470.17153910726668856No Hit
GATACCACTGCTTCCCATGTACTCT460.16788933902697178No Hit
ACATGGGAAGCAGTGGTATCAACGC420.15329026606810467No Hit
GTATCAACGCAGAGTACTTTTTTTT420.15329026606810467No Hit
CCACTGCTTCCCATGTACTCTGCGT420.15329026606810467No Hit
GNGTACATGGGAAGCAGTGGTATCA410.1496404978283879No Hit
GAACAAAAAAAAAAAAAAAAAAAAA390.14234096134895435No Hit
GCAGTGGTATCAACGCAGAGTACAT380.13869119310923758No Hit
ATGGGAAGCAGTGGTATCAACGCAG360.131391656629804No Hit
GTTGATACCACTGCTTCCCATGTAC360.131391656629804No Hit
GGGAAGCAGTGGTATCAACGCAGAG360.131391656629804No Hit
CTTCCCATGTACTCTGCGTTGATAC350.1277418883900872No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA340.12409212015037044No Hit
GGTATCAACGCAGAGTACTTTTTTT340.12409212015037044No Hit
CNTGTACTCTGCGTTGATACCACTG340.12409212015037044No Hit
CCCNTGTACTCTGCGTTGATACCAC340.12409212015037044No Hit
TACCACTGCTTCCCATGTACTCTGC320.1167925836709369No Hit
NNANNNNAAAANNAAAAAAAAAANA290.10584327895178655No Hit
AAGCAGTGGTATCAACGCAGAGTAC290.10584327895178655No Hit
CCATGTACTCTGCGTTGATACCACT280.10219351071206978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACAT2901.6734703E-109.4418172
GAGTACA2852.1464075E-109.3267261
ATACCAC2302.7121132E-97.72219819
GATACCA2406.208211E-97.40043918
TGATACC2506.384653E-107.36990217
GAAGCAG2158.704119E-77.306368411
GGAAGCA2158.704119E-77.306368410
TTGATAC2503.425157E-97.1901816
GGGAAGC2251.7884231E-66.9816419
AGCAGTG2502.2622226E-76.898137613
GTTGATA2752.3179382E-86.53652715
AAGCAGT2501.7171224E-66.535077612
AGTGGTA2808.456482E-96.516507614
GTATCAA2602.1786036E-76.505880418
CGTTGAT2702.9262083E-86.464039314
GTGGTAT2854.712456E-86.307174715
CATGGGA3103.4971326E-66.2385074
TACATGG3454.397316E-76.2284614
GCAGTGG2554.9899063E-76.2220714
GTACATG3554.9784285E-76.18283463
Produced by FastQC (version 0.11.5)