FastQCFastQC Report
Thu 26 May 2016
SRR1926626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27399
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC11524.204533012153728No Hit
GTACATGGGAAGCAGTGGTATCAAC10523.839556188182051No Hit
GTATCAACGCAGAGTACTTTTTTTT5191.8942297164130077No Hit
GAGTACATGGGAAGCAGTGGTATCA4571.6679440855505676No Hit
CATGTACTCTGCGTTGATACCACTG4321.5766998795576481No Hit
ACGCAGAGTACATGGGAAGCAGTGG3821.3942114675718091No Hit
GGTATCAACGCAGAGTACTTTTTTT3711.3540640169349245No Hit
GCGTTGATACCACTGCTTCCCATGT3391.2372714332639878No Hit
TATCAACGCAGAGTACTTTTTTTTT2741.0000364976823972No Hit
ACTCTGCGTTGATACCACTGCTTCC2730.9963867294426804No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2580.9416402058469288No Hit
GGTATCAACGCAGAGTACATGGGAA2480.9051425234497609No Hit
GTATCAACGCAGAGTACATGGGAAG2410.8795941457717436No Hit
TATCAACGCAGAGTACATGGGAAGC2320.8467462316142924No Hit
CATGGGAAGCAGTGGTATCAACGCA1830.6679075878681704No Hit
GCTTCCCATGTACTCTGCGTTGATA1740.6350596737107194No Hit
ACATGGGAAGCAGTGGTATCAACGC1550.5657140771561006No Hit
CAGTGGTATCAACGCAGAGTACATG1520.5547647724369503No Hit
GTGGTATCAACGCAGAGTACATGGG1500.5474652359575167No Hit
GATACCACTGCTTCCCATGTACTCT1360.49636848060148175No Hit
ATACCACTGCTTCCCATGTACTCTG1320.48176940764261467No Hit
GTACTCTGCGTTGATACCACTGCTT1300.4744698711631811No Hit
GCAGTGGTATCAACGCAGAGTACAT1250.4562210299645973No Hit
GCAGAGTACATGGGAAGCAGTGGTA1140.41607357932771266No Hit
CTTCCCATGTACTCTGCGTTGATAC1090.39782473812912883No Hit
CTGCTTCCCATGTACTCTGCGTTGA1080.39417496988941203No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1040.3795758969305449No Hit
GGGAAGCAGTGGTATCAACGCAGAG1040.3795758969305449No Hit
ATGGGAAGCAGTGGTATCAACGCAG1030.37592612869082814No Hit
CCACTGCTTCCCATGTACTCTGCGT920.33577867805394357No Hit
AAGCAGTGGTATCAACGCAGAGTAC900.32847914157451No Hit
GTTGATACCACTGCTTCCCATGTAC890.3248293733347932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA840.30658053213620934No Hit
GTACTTTTTTTTTTTTTTTTTTTTT800.29198145917734225No Hit
GCAGAGTACTTTTTTTTTTTTTTTT780.2846819226979087No Hit
CCATGTACTCTGCGTTGATACCACT780.2846819226979087No Hit
TACCACTGCTTCCCATGTACTCTGC750.27373261797875836No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA580.2116865579035731No Hit
ATCAACGCAGAGTACTTTTTTTTTT580.2116865579035731No Hit
ATCAACGCAGAGTACATGGGAAGCA570.20803678966385633No Hit
GTGGTATCAACGCAGAGTACTTTTT510.18613818022555567No Hit
ACCATGTACTCTGCGTTGATACCAC420.15329026606810467No Hit
AAAGTACTCTGCGTTGATACCACTG380.13869119310923758No Hit
GGAAGCAGTGGTATCAACGCAGAGT380.13869119310923758No Hit
TGATACCACTGCTTCCCATGTACTC380.13869119310923758No Hit
CTGCGTTGATACCACTGCTTCCCAT370.1350414248695208No Hit
CTCTGCGTTGATACCACTGCTTCCC360.131391656629804No Hit
AAAAAGTACTCTGCGTTGATACCAC360.131391656629804No Hit
TTCCCATGTACTCTGCGTTGATACC330.12044235191065367No Hit
GAGTAAGCAGTGGTATCAACGCAGA310.11314281543122011No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA290.10584327895178655No Hit
TCAACGCAGAGTACATGGGAAGCAG280.10219351071206978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA3050.09.052852
TGATACC3650.08.31674617
CCCATGT3400.08.1060651
TACTCTG3600.07.9342517
GATACCA3850.07.88470718
TTGATAC3750.07.841998616
TGCGTTG3850.07.6383112
ATACCAC4000.07.58903119
CATGTAC3650.07.56470973
GTACTCT3800.07.5166596
ATGTACT3700.07.46248444
CTGCGTT3850.07.39191311
TGTACTC3651.8189894E-127.3038585
TCTGCGT3900.07.29714610
GCTTCCC1701.1014253E-47.25422115
CTCTGCG3950.07.21797139
ACTCTGC4000.07.12774758
GTTGATA4250.06.919410715
TCCCATG1802.228761E-46.85120818
TGCTTCC1802.228761E-46.85120814
Produced by FastQC (version 0.11.5)