FastQCFastQC Report
Thu 26 May 2016
SRR1926625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23966
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA4331.8067261954435452No Hit
CCCATGTACTCTGCGTTGATACCAC3221.3435700575815739No Hit
GTACATGGGAAGCAGTGGTATCAAC3181.3268797463072686No Hit
GAGTACATGGGAAGCAGTGGTATCA1500.6258866727864475No Hit
CATGTACTCTGCGTTGATACCACTG1160.4840190269548527No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA950.39639489276475004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA930.38804973712759744No Hit
GCGTTGATACCACTGCTTCCCATGT910.3797045814904448No Hit
ACTCTGCGTTGATACCACTGCTTCC820.34215138112325794No Hit
GGTATCAACGCAGAGTACATGGGAA790.329633647667529No Hit
GTATCAACGCAGAGTACATGGGAAG770.3212884920303764No Hit
GNACATGGGAAGCAGTGGTATCAAC760.3171159142118No Hit
ACGCAGAGTACATGGGAAGCAGTGG740.3087707585746474No Hit
AAACAAAAAAAAAAAAAAAAAAAAA680.2837352916631895No Hit
CNCATGTACTCTGCGTTGATACCAC680.2837352916631895No Hit
TATCAACGCAGAGTACATGGGAAGC650.2712175582074606No Hit
CATGGGAAGCAGTGGTATCAACGCA480.2002837352916632No Hit
GCTTCCCATGTACTCTGCGTTGATA460.19193857965451055No Hit
GTGGTATCAACGCAGAGTACATGGG420.1752482683802053No Hit
CAGTGGTATCAACGCAGAGTACATG420.1752482683802053No Hit
GTACTCTGCGTTGATACCACTGCTT420.1752482683802053No Hit
GNGTACATGGGAAGCAGTGGTATCA370.1543853792873237No Hit
GTATCAACGCAGAGTACTTTTTTTT360.15021280146874738No Hit
ATACCACTGCTTCCCATGTACTCTG360.15021280146874738No Hit
CTGCTTCCCATGTACTCTGCGTTGA360.15021280146874738No Hit
GGTATCAACGCAGAGTACTTTTTTT350.14604022365017108No Hit
TACCACTGCTTCCCATGTACTCTGC350.14604022365017108No Hit
GATACCACTGCTTCCCATGTACTCT340.14186764583159475No Hit
CTTCCCATGTACTCTGCGTTGATAC310.12934991237586582No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA300.1251773345572895No Hit
GGGAAGCAGTGGTATCAACGCAGAG300.1251773345572895No Hit
GCAGTGGTATCAACGCAGAGTACAT290.12100475673871318No Hit
ACATGGGAAGCAGTGGTATCAACGC280.11683217892013686No Hit
ACACAAAAAAAAAAAAAAAAAAAAA280.11683217892013686No Hit
GAACAAAAAAAAAAAAAAAAAAAAA280.11683217892013686No Hit
NNANNNNAAAANNAAAAAAAAAANA270.11265960110156054No Hit
GNTATCAACGCAGAGTACATGGGAA260.10848702328298424No Hit
CNTGTACTCTGCGTTGATACCACTG260.10848702328298424No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA260.10848702328298424No Hit
CCACTGCTTCCCATGTACTCTGCGT240.1001418676458316No Hit
ATGGGAAGCAGTGGTATCAACGCAG240.1001418676458316No Hit
GTTGATACCACTGCTTCCCATGTAC240.1001418676458316No Hit
GCAGAGTACATGGGAAGCAGTGGTA240.1001418676458316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA1409.528867E-711.8318682
CCCATGT1401.3855151E-611.4786781
ATACCAC1351.9372237E-910.63407419
GATACCA1301.2178134E-810.41799618
ATGTACT1701.7127604E-810.143794
GTACTCT1754.503454E-89.5388716
TGTACTC1754.503454E-89.5388715
TGATACC1354.8716174E-89.53728717
CATGTAC1906.8714144E-89.2709073
TACTCTG1652.989218E-79.2428537
CTCTGCG1503.2721255E-79.2025649
ACTCTGC1603.685218E-79.1183948
TGCGTTG1503.9498082E-79.08607612
TTGATAC1451.1050906E-79.04172616
CTGCGTT1608.3323357E-78.62740311
TCTGCGT1608.3323357E-78.62740310
TACATGG2807.903327E-88.3809062
GTACATG3056.148184E-98.3424271
GCGTTGA1651.5639725E-68.26006913
GTTGATA1604.8168295E-78.21281415
Produced by FastQC (version 0.11.5)