##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23966 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.56442460151882 26.0 2.0 30.0 2.0 31.0 2 14.484269381623967 7.0 2.0 30.0 2.0 31.0 3 17.876449970791956 16.0 2.0 30.0 2.0 31.0 4 19.474922807310357 25.0 2.0 33.0 2.0 35.0 5 20.249019444212635 27.0 2.0 35.0 2.0 35.0 6 20.574188433614285 28.0 2.0 35.0 2.0 37.0 7 20.67983810398064 28.0 2.0 35.0 2.0 37.0 8 20.720812818159057 27.0 2.0 35.0 2.0 37.0 9 22.85412667946257 29.0 2.0 37.0 2.0 39.0 10 23.51606442460152 30.0 2.0 37.0 2.0 39.0 11 20.87177668363515 27.0 2.0 35.0 2.0 39.0 12 22.050154385379287 29.0 2.0 35.0 2.0 39.0 13 19.031294333639323 21.0 2.0 35.0 2.0 39.0 14 21.954226821330217 27.0 2.0 36.0 2.0 40.0 15 22.803388133188683 30.0 2.0 37.0 2.0 40.0 16 23.089251439539346 30.0 2.0 37.0 2.0 40.0 17 22.545022114662437 30.0 2.0 37.0 2.0 40.0 18 22.610531586414087 30.0 2.0 36.0 2.0 40.0 19 22.768421931069014 30.0 2.0 37.0 2.0 40.0 20 22.989443378119002 30.0 2.0 37.0 2.0 40.0 21 21.022698823333055 28.0 2.0 35.0 2.0 40.0 22 21.857548193273804 30.0 2.0 36.0 2.0 40.0 23 21.785070516565135 30.0 2.0 37.0 2.0 40.0 24 20.957523157806893 29.0 2.0 37.0 2.0 40.0 25 20.721855962613702 28.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.505247901449 1101 2 10.437709990141444 1101 3 7.304725083572659 1101 4 9.023365106813632 1101 5 8.227466471388542 1101 6 7.475931431916337 1101 7 9.360478091303236 1101 8 6.298021760761063 1101 9 6.7609846400467575 1101 10 7.134779772534845 1101 11 8.563193809696706 1101 12 6.332736032524629 1101 13 10.95637729035575 1101 14 9.238687109679805 1101 15 8.435008483384358 1101 16 8.605076820347772 1101 17 2.1314940170884036 1101 18 6.1791073469131526 1101 19 7.3464093883562 1101 20 4.525611919112865 1101 21 2.139933383609403 1101 22 5.960364140030503 1101 23 6.836154689900212 1101 24 8.474841871146044 1101 25 9.09370150133703 1102 1 5.93620028240138 1102 2 9.38890046633192 1102 3 6.399963178810754 1102 4 7.149555583004105 1102 5 7.628656947579017 1102 6 6.878312384297288 1102 7 6.722382853208 1102 8 6.724212236951537 1102 9 5.52288940195152 1102 10 4.103827391582467 1102 11 7.187003333506233 1102 12 6.157736032524628 1102 13 8.88732967130813 1102 14 6.762496633489331 1102 15 3.6207227690986414 1102 16 4.7896006298715825 1102 17 5.354113064707452 1102 18 5.719583537389344 1102 19 2.9214093883561993 1102 20 4.241088109589054 1102 21 4.017314335990356 1102 22 4.322268901935267 1102 23 4.605202308947831 1102 24 6.7081752044793745 1102 25 6.119891977527505 1103 1 3.3978281893781244 1103 2 7.611574884936571 1103 3 5.911591085787499 1103 4 6.999555583004106 1103 5 5.785052296416225 1103 6 6.32366122150659 1103 7 5.296801457859161 1103 8 5.192816888114329 1103 9 3.569401029858497 1103 10 4.079408786931303 1103 11 4.889328914901579 1103 12 4.216456962757189 1103 13 7.5931436247965 1103 14 5.1462175637218905 1103 15 4.3137460249125965 1103 16 4.077391327546 1103 17 2.5860898088934974 1103 18 1.2067928397149252 1103 19 3.0440838069608525 1103 20 4.053297411914638 1103 21 5.696965498781054 1103 22 2.338547971702706 1103 23 4.4552023089478325 1103 24 6.052361250991005 1103 25 1.2908222100856435 1104 1 7.019533615734712 1104 2 8.802789355220808 1104 3 6.888852067699641 1104 4 9.088444471892995 1104 5 8.514768058690127 1104 6 7.550534606519513 1104 7 7.289049519874666 1104 8 7.307545570284873 1104 9 6.300667179729299 1104 10 5.820494058249132 1104 11 7.453670000172899 1104 12 4.466069365857962 1104 13 8.092885226863686 1104 14 6.706941077933774 1104 15 6.576278324654197 1104 16 5.559045074316028 1104 17 6.234668620263008 1104 18 4.014027981833788 1104 19 4.613076055022866 1104 20 2.74942144292239 1104 21 1.692314335990357 1104 22 4.088935568601933 1104 23 5.277424531170055 1104 24 5.841508537812711 1104 25 4.947669755305281 1105 1 4.349614916547722 1105 2 9.230973637063626 1105 3 7.765816837347341 1105 4 7.780043387882156 1105 5 4.15487645977414 1105 6 6.489287994053388 1105 7 7.6955535849153165 1105 8 5.530309797927149 1105 9 6.0594747678051775 1105 10 4.290412757436123 1105 11 6.855296016433062 1105 12 5.283955544719753 1105 13 5.532451622527642 1105 14 3.262496633489331 1105 15 5.513405695927911 1105 16 5.524356727432558 1105 17 6.435210625683062 1105 18 3.832998171535685 1105 19 4.151287437136688 1105 20 3.8502344510524686 1105 21 5.702070433551331 1105 22 6.441781097057216 1105 23 1.9673974308990516 1105 24 6.235004472772058 1105 25 5.545501733625066 1106 1 8.351316561471148 1106 2 11.518551629122618 1106 3 8.260428295089824 1106 4 9.860020699283176 1106 5 9.133889505718553 1106 6 8.858544942436826 1106 7 8.389824713673114 1106 8 7.4486308416027 1106 9 7.081028936835242 1106 10 6.870106461349906 1106 11 8.842817286994602 1106 12 8.379247660431606 1106 13 3.8722133922383613 1106 14 6.169473377675377 1106 15 6.709094862121898 1106 16 7.961112257778559 1106 17 7.237252599591173 1106 18 9.323071909482366 1106 19 8.695246597658524 1106 20 7.495157877030916 1106 21 8.46440735924617 1106 22 8.547850297284104 1106 23 9.455202308947833 1106 24 9.959337995177052 1106 25 9.895473372876342 1107 1 3.347738743939839 1107 2 -12.086099533668081 1107 3 -5.471831692984118 1107 4 2.127760711209234 1107 5 3.2549389988610677 1107 6 3.4667739227588292 1107 7 3.365972596797743 1107 8 3.543443006182308 1107 9 1.8049406840028013 1107 10 1.6769043146593887 1107 11 4.151105897608797 1107 12 0.6660693658579611 1107 13 4.439893773872232 1107 14 2.9291633001559987 1107 15 2.4258509742268473 1107 16 2.5043442196151737 1107 17 3.012446398040783 1107 18 -1.6560574882516832 1107 19 1.525896567843379 1107 20 2.9596778531787997 1107 21 -1.7794805358045167 1107 22 1.5812432609096234 1107 23 0.8654587192042413 1107 24 3.026123922428095 1107 25 3.7493791570146833 1108 1 6.314771710972806 1108 2 11.03294808537954 1108 3 1.5190107978583747 1108 4 7.951936535385059 1108 5 8.65603789995997 1108 6 6.833074289059194 1108 7 8.670001900827046 1108 8 7.678974141713443 1108 9 5.689556068618188 1108 10 3.9681131058681807 1108 11 7.444146190649089 1108 12 2.6184503182389136 1108 13 3.45637729035575 1108 14 3.572020443013141 1108 15 3.3397703881462633 1108 16 3.9860292013001555 1108 17 2.3933987789931663 1108 18 3.4648216326274373 1108 19 3.179742721689532 1108 20 3.216088109589055 1108 21 3.1637429074189285 1108 22 0.5079831876495504 1108 23 3.2885356422811647 1108 24 3.9272228235269964 1108 25 4.09370150133703 1109 1 1.3862002824013793 1109 2 -12.086099533668081 1109 3 -5.836878926452403 1109 4 1.5785029514251576 1109 5 1.782604316000068 1109 6 1.1125229106130803 1109 7 3.385540747944841 1109 8 2.721580658004168 1109 9 2.049205191425205 1109 10 0.3064589705298353 1109 11 0.6133191229799166 1109 12 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7.749379157014683 1111 1 5.6303863289130085 1111 2 7.751109768657502 1111 3 6.748800388113079 1111 4 7.697230001608759 1111 5 7.319936017346457 1111 6 7.486451919181011 1111 7 6.715406109021952 1111 8 7.123049446253862 1111 9 5.755447541486404 1111 10 6.451501810187118 1111 11 8.30793356606437 1111 12 7.216456962757189 1111 13 8.872213392238361 1111 14 6.913659424187006 1111 15 7.453280908633527 1111 16 5.844833188011119 1111 17 7.307020041451636 1111 18 6.834699816459111 1111 19 6.788269853472478 1111 20 5.030041597961148 1111 21 7.603942242967101 1111 22 7.361803785656196 1111 23 7.408690681040856 1111 24 8.37794264633984 1111 25 8.47686872171355 1112 1 0.5203466238647927 1112 2 9.084632173648993 1112 3 7.643865617835143 1112 4 0.4873604610528872 1112 5 6.203656947579017 1112 6 5.342946530638752 1112 7 7.6955535849153165 1112 8 7.6522610174393435 1112 9 7.327767450732008 1112 10 6.241632269631246 1112 11 3.245539918872087 1112 12 6.0644433495977985 1112 13 11.239768695698373 1112 14 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4.928312384297289 2204 7 5.697382853207998 2204 8 4.974212236951537 2204 9 4.0978894019515195 2204 10 3.6288273915824654 2204 11 5.262003333506232 2204 12 5.7327360325246275 2204 13 4.76232967130813 2204 14 5.08749663348933 2204 15 5.020722769098644 2204 16 4.5396006298715825 2204 17 -0.7958869352925504 2204 18 1.769583537389341 2204 19 2.8714093883561986 2204 20 1.1160881095890538 2204 21 2.292314335990355 2204 22 3.822268901935267 2204 23 2.605202308947831 2204 24 4.733175204479377 2204 25 4.969891977527503 2205 1 3.258293305657194 2205 2 7.867388838424942 2205 3 5.516242248578195 2205 4 5.162346280678523 2205 5 4.29668020339297 2205 6 6.719010058715895 2205 7 4.250289829952184 2205 8 5.634677353230607 2205 9 3.9414940531143117 2205 10 3.9863855311173495 2205 11 4.005607984669023 2205 12 4.193201148803698 2205 13 7.011748275959292 2205 14 6.029938493954447 2205 15 4.941653001656782 2205 16 3.542507606615768 2205 17 3.4930665530795437 2205 18 3.2765602815753887 2205 19 2.509200086030617 2205 20 1.7974834584262638 2205 21 -2.4658251988933664 2205 22 0.31529215774921937 2205 23 1.524969750808296 2205 24 2.6337565998282138 2205 25 3.0117524426437825 2206 1 2.536200282401378 2206 2 5.3389004663319195 2206 3 3.824963178810755 2206 4 1.1245555830041063 2206 5 5.378656947579017 2206 6 4.803312384297289 2206 7 4.6223828532079985 2206 8 4.699212236951539 2206 9 1.8978894019515202 2206 10 3.2538273915824654 2206 11 1.412003333506231 2206 12 3.8827360325246296 2206 13 6.562329671308131 2206 14 1.9124966334893294 2206 15 2.7707227690986436 2206 16 3.014600629871584 2206 17 3.554113064707451 2206 18 -0.13041646261065765 2206 19 1.4964093883561986 2206 20 1.2410881095890538 2206 21 1.6173143359903541 2206 22 -2.5277310980647343 2206 23 -2.719797691052168 2206 24 0.808175204479376 2206 25 1.6198919775275051 2207 1 0.8862002824013793 2207 2 -12.086099533668081 2207 3 -5.29448126563369 2207 4 -4.000444416995894 2207 5 0.5647680586901274 2207 6 1.9283123842972891 2207 7 2.59460507543022 2207 8 3.6908789036182057 2207 9 0.6617782908404095 2207 10 0.0593829471380225 2207 11 -3.024107777604879 2207 12 -3.278375078586482 2207 13 0.7817741157525724 2207 14 -4.015281144288448 2207 15 -1.0292772309013571 2207 16 -1.0020660367950853 2207 17 -0.4319980464036597 2207 18 -0.4637497959439898 2207 19 -0.9313683894215785 2207 20 -0.9228007792998341 2207 21 -5.379907886231866 2207 22 -4.583286653620291 2207 23 -3.1003532466077246 2207 24 -2.025158128853956 2207 25 -1.0967746891391634 2208 1 0.2582933056571939 2208 2 2.5650632570295944 2208 3 2.213916667182847 2208 4 3.022811396957593 2208 5 0.8083081103697154 2208 6 0.41668447732054403 2208 7 1.924708434603346 2208 8 0.14630526020735246 2208 9 -2.221296644560109 2208 10 -2.1298935386500943 2208 11 -0.31997341067981466 2208 12 1.0769220790362581 2208 13 2.1745389736337124 2208 14 -3.9700615060455533 2208 15 -1.7792772309013571 2208 16 -1.0853993701284175 2208 17 -2.041817167850688 2208 18 -3.32809088121531 2208 19 -2.816381309318217 2208 20 -1.6676328206435045 2208 21 0.04580270808337872 2208 22 -1.5451729585298501 2208 23 -4.405262807331237 2208 24 -3.8313596792415545 2208 25 -3.546387092239936 2209 1 0.99731139351249 2209 2 1.8472337996652524 2209 3 2.911074289921867 2209 4 -1.4893333058847809 2209 5 2.3147680586901274 2209 6 2.9949790509639556 2209 7 3.5112717420968877 2209 8 2.463101125840428 2209 9 1.3006671797292988 2209 10 0.9316051693602425 2209 11 -3.079663333160436 2209 12 1.488291588080184 2209 13 2.7817741157525724 2209 14 1.6180521890448851 2209 15 1.5318338802097529 2209 16 0.981267296538249 2209 17 -0.7208869352925475 2209 18 1.147361315167121 2209 19 1.3686316105784222 2209 20 1.5716436651446095 2209 21 3.714536558212579 2209 22 2.3333800130463764 2209 23 -5.455908802163279 2209 24 -9.536269239965069 2209 25 -5.585663578028051 2210 1 0.28620028240137785 2210 2 -12.086099533668081 2210 3 -5.942893964046389 2210 4 0.028127011575534766 2210 5 0.8893712332933035 2210 6 1.1283123842972884 2210 7 1.3366685674937138 2210 8 -18.225787763048462 2210 9 -10.305682026619909 2210 10 -4.589029751274676 2210 11 -9.355853809350911 2210 12 -1.0101211103325127 2210 13 -2.9198131858347285 2210 14 -1.4517890807963845 2210 15 -3.35070580232993 2210 16 -2.628256512985562 2210 17 -2.435172649578263 2210 18 -2.5732736054678007 2210 19 -7.853590611643799 2210 20 -4.726769033268088 2210 21 -3.5362570925810743 2210 22 -8.949159669493305 2210 23 -6.516226262480739 2210 24 -7.606110509806339 2210 25 -5.830108022472496 2211 1 0.9593710141086973 2211 2 5.084632173648993 2211 3 1.0828900080790476 2211 4 3.0483360708089826 2211 5 1.5939008500180414 2211 6 0.14782457941923965 2211 7 -18.377617146792 2211 8 -11.884324348414316 2211 9 -7.3307691346338455 2211 10 -5.977879925490704 2211 11 -9.315435690884012 2211 12 -3.9599468943046396 2211 13 -6.345597157960164 2211 14 -4.103357025047256 2211 15 -4.559765035779407 2211 16 -11.50003351646988 2211 17 -5.6867405938291355 2211 18 -4.581635974805781 2211 19 -7.214566221399897 2211 20 -4.515619207484114 2211 21 -2.639392981082814 2211 22 -3.3387067078208332 2211 23 -3.6423586666619236 2211 24 -18.691824795520624 2211 25 -18.430108022472496 2212 1 1.3983954043525983 2212 2 -12.086099533668081 2212 3 -5.819549016311196 2212 4 -17.000444416995894 2212 5 -17.796343052420983 2212 6 -18.07168761570271 2212 7 -7.523958610206636 2212 8 -6.811153616706999 2212 9 -3.160037427316773 2212 10 -2.441294559637047 2212 11 -3.0715332518596234 2212 12 0.4790774959392614 2212 13 -2.369987401862602 2212 14 -0.8594545860228635 2212 15 -0.8280577187062335 2212 16 -0.6951554676893927 2212 17 -0.46722839870718147 2212 18 0.2964128056820243 2212 19 -0.38529792871696955 2212 20 -0.4424484757767999 2212 21 2.1898753116001117 2212 22 0.3930006092523399 2212 23 0.3332510894356382 2212 24 0.6984191069184007 2212 25 -0.2837665590578631 2213 1 -16.11379971759862 2213 2 -12.086099533668081 2213 3 -8.31432253547496 2213 4 -5.371872988424466 2213 5 -17.796343052420983 2213 6 -9.014544758559854 2213 7 -18.377617146792 2213 8 -9.511502048762749 2213 9 -7.2771105980484805 2213 10 -5.8747440369889645 2213 11 -4.898710952208054 2213 12 -4.810121110332513 2213 13 -16.66267032869187 2213 14 -10.880360509367812 2213 15 -6.493562945187072 2213 16 -5.999685084414132 2213 17 -4.520886935292548 2213 18 -4.344702176896373 2213 19 -5.167876325929516 2213 20 -4.5267690332680885 2213 21 -3.6219713782953598 2213 22 -4.263445383779018 2213 23 -3.0305119767664515 2213 24 -2.8918247955206233 2213 25 -3.6586794510439233 2214 1 -16.11379971759862 2214 2 -12.086099533668081 2214 3 -8.941500235823392 2214 4 -17.000444416995894 2214 5 -17.796343052420983 2214 6 -18.07168761570271 2214 7 -13.206885439474927 2214 8 -10.323348738658218 2214 9 -8.47711059804848 2214 10 -7.782757974271194 2214 11 -10.217874715274256 2214 12 -6.691654211377809 2214 13 -16.66267032869187 2214 14 -12.20091800065701 2214 15 -20.779277230901357 2214 16 -13.353692053055246 2214 17 -20.32088693529255 2214 18 -12.849928657732608 2214 19 -8.970663782375507 2214 20 -7.320497256264604 2214 21 -4.371100298155987 2214 22 -5.3143164639183915 2214 23 -5.105773300808266 2214 24 -4.204019917471843 2214 25 -3.8691324127163966 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 6375.0 3 1.0 4 34.0 5 125.0 6 179.0 7 152.0 8 142.0 9 94.0 10 114.0 11 93.0 12 97.0 13 98.0 14 122.0 15 258.0 16 333.0 17 433.0 18 392.0 19 450.0 20 512.0 21 610.0 22 667.0 23 682.0 24 668.0 25 698.0 26 540.0 27 500.0 28 495.0 29 530.0 30 539.0 31 598.0 32 744.0 33 943.0 34 1086.0 35 1285.0 36 1267.0 37 1278.0 38 832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99213477690926 21.634218575520705 9.918920231101616 22.454726416468418 2 14.311270125223613 26.45597296760088 31.643808387994433 27.588948519181077 3 21.136625587374766 33.12350385672489 24.02695274403759 21.712917811862752 4 12.450092511442206 23.73648846041484 28.327977407732007 35.485441620410946 5 12.341587054006629 43.52700331448626 30.01559758237473 14.115812049132384 6 30.216574367088604 34.938686708860764 16.697982594936708 18.146756329113924 7 23.738954344624446 37.95563328424153 19.297680412371136 19.007731958762886 8 27.018479193963763 36.83187999819275 18.370758595761984 17.778882212081506 9 26.796976151693606 22.202731487282296 16.10491584179092 34.89537651923318 10 17.509859632755184 35.723675840719224 25.507824095670244 21.25864043085535 11 27.90316144193677 31.18875537622489 17.416751651664967 23.49133153017337 12 23.85991322057912 35.07039759142832 23.160364827769413 17.90932436022315 13 28.02007528230866 27.18193224592221 20.015056461731493 24.78293601003764 14 21.3072284039752 32.29423256604094 20.997196976131825 25.401342053852034 15 30.4391045034143 29.341343244878555 19.124384130002593 21.095168121704553 16 18.906330860790064 33.090391581852685 26.479213386234257 21.524064171122994 17 23.302815684919675 33.900500950077735 23.08688892727587 19.709794437726725 18 23.920054315539335 30.060256301451243 25.299159806500892 20.72052957650853 19 22.15342860777745 34.97731224290742 25.0922352741614 17.777023875153724 20 23.57590966122961 31.551652028439985 23.345880384776244 21.52655792555416 21 20.19743164147812 32.584956757228966 25.20310998514895 22.014501616143967 22 21.608531248672303 32.285337978501936 20.19373751964991 25.912393253175853 23 18.84893288226204 33.18715282128388 24.453911372843837 23.51000292361024 24 21.142672692059396 36.571981923821824 21.110393802453196 21.174951581665592 25 21.39665037798104 30.718790460677443 19.801194503612745 28.083364657728772 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 849.0 1 849.0 2 746.5 3 644.0 4 644.0 5 644.0 6 588.5 7 533.0 8 533.0 9 533.0 10 466.0 11 399.0 12 399.0 13 399.0 14 366.5 15 334.0 16 334.0 17 334.0 18 361.0 19 388.0 20 388.0 21 388.0 22 462.5 23 537.0 24 537.0 25 537.0 26 686.5 27 836.0 28 836.0 29 836.0 30 1022.0 31 1208.0 32 1208.0 33 1208.0 34 1311.0 35 1414.0 36 1414.0 37 1414.0 38 1579.0 39 1744.0 40 1744.0 41 1744.0 42 2151.5 43 2559.0 44 2559.0 45 2559.0 46 3352.5 47 4146.0 48 4146.0 49 4146.0 50 3663.5 51 3181.0 52 3181.0 53 3181.0 54 2485.0 55 1789.0 56 1789.0 57 1789.0 58 1536.5 59 1284.0 60 1284.0 61 1284.0 62 1091.5 63 899.0 64 899.0 65 899.0 66 753.5 67 608.0 68 608.0 69 608.0 70 486.5 71 365.0 72 365.0 73 365.0 74 259.0 75 153.0 76 153.0 77 153.0 78 111.0 79 69.0 80 69.0 81 69.0 82 45.5 83 22.0 84 22.0 85 22.0 86 13.5 87 5.0 88 5.0 89 5.0 90 2.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.057414670783611 2 37.023282984227656 3 5.874989568555454 4 14.303596762079613 5 14.39539347408829 6 15.613786197112574 7 9.338229157973796 8 7.648335141450388 9 0.09596928982725528 10 1.6064424601518819 11 5.895852457648335 12 5.758157389635317 13 16.861386964866895 14 1.7524826838020529 15 3.45489443378119 16 3.2462655428523743 17 3.3797880330468164 18 1.6690311274305265 19 1.6064424601518819 20 0.23366435784027373 21 4.473003421513811 22 1.7900358841692399 23 0.09596928982725528 24 9.513477426354001 25 0.09596928982725528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 23966.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.69615288325127 #Duplication Level Percentage of deduplicated Percentage of total 1 94.4779935803275 72.46098639739631 2 3.062945432783853 4.698322623716932 3 0.8106196616070942 1.8651422849036134 4 0.42435123225069366 1.3018442793958107 5 0.23937761819269898 0.9179671200867896 6 0.16321201240411295 0.751064007343737 7 0.10880800826940863 0.5841608946006843 8 0.0707252053751156 0.43394809313193694 9 0.09248680702899734 0.6384044062421764 >10 0.4624340351449867 7.139280647584077 >50 0.059844404548174746 3.6217975465242427 >100 0.027202002067352158 5.587081699073688 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 433 1.8067261954435452 No Hit CCCATGTACTCTGCGTTGATACCAC 322 1.3435700575815739 No Hit GTACATGGGAAGCAGTGGTATCAAC 318 1.3268797463072686 No Hit GAGTACATGGGAAGCAGTGGTATCA 150 0.6258866727864475 No Hit CATGTACTCTGCGTTGATACCACTG 116 0.4840190269548527 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 95 0.39639489276475004 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 93 0.38804973712759744 No Hit GCGTTGATACCACTGCTTCCCATGT 91 0.3797045814904448 No Hit ACTCTGCGTTGATACCACTGCTTCC 82 0.34215138112325794 No Hit GGTATCAACGCAGAGTACATGGGAA 79 0.329633647667529 No Hit GTATCAACGCAGAGTACATGGGAAG 77 0.3212884920303764 No Hit GNACATGGGAAGCAGTGGTATCAAC 76 0.3171159142118 No Hit ACGCAGAGTACATGGGAAGCAGTGG 74 0.3087707585746474 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 68 0.2837352916631895 No Hit CNCATGTACTCTGCGTTGATACCAC 68 0.2837352916631895 No Hit TATCAACGCAGAGTACATGGGAAGC 65 0.2712175582074606 No Hit CATGGGAAGCAGTGGTATCAACGCA 48 0.2002837352916632 No Hit GCTTCCCATGTACTCTGCGTTGATA 46 0.19193857965451055 No Hit GTGGTATCAACGCAGAGTACATGGG 42 0.1752482683802053 No Hit CAGTGGTATCAACGCAGAGTACATG 42 0.1752482683802053 No Hit GTACTCTGCGTTGATACCACTGCTT 42 0.1752482683802053 No Hit GNGTACATGGGAAGCAGTGGTATCA 37 0.1543853792873237 No Hit GTATCAACGCAGAGTACTTTTTTTT 36 0.15021280146874738 No Hit ATACCACTGCTTCCCATGTACTCTG 36 0.15021280146874738 No Hit CTGCTTCCCATGTACTCTGCGTTGA 36 0.15021280146874738 No Hit GGTATCAACGCAGAGTACTTTTTTT 35 0.14604022365017108 No Hit TACCACTGCTTCCCATGTACTCTGC 35 0.14604022365017108 No Hit GATACCACTGCTTCCCATGTACTCT 34 0.14186764583159475 No Hit CTTCCCATGTACTCTGCGTTGATAC 31 0.12934991237586582 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 30 0.1251773345572895 No Hit GGGAAGCAGTGGTATCAACGCAGAG 30 0.1251773345572895 No Hit GCAGTGGTATCAACGCAGAGTACAT 29 0.12100475673871318 No Hit ACATGGGAAGCAGTGGTATCAACGC 28 0.11683217892013686 No Hit ACACAAAAAAAAAAAAAAAAAAAAA 28 0.11683217892013686 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 28 0.11683217892013686 No Hit NNANNNNAAAANNAAAAAAAAAANA 27 0.11265960110156054 No Hit GNTATCAACGCAGAGTACATGGGAA 26 0.10848702328298424 No Hit CNTGTACTCTGCGTTGATACCACTG 26 0.10848702328298424 No Hit GNAAAAAAAAAAAAAAAAAAAAAAA 26 0.10848702328298424 No Hit CCACTGCTTCCCATGTACTCTGCGT 24 0.1001418676458316 No Hit ATGGGAAGCAGTGGTATCAACGCAG 24 0.1001418676458316 No Hit GTTGATACCACTGCTTCCCATGTAC 24 0.1001418676458316 No Hit GCAGAGTACATGGGAAGCAGTGGTA 24 0.1001418676458316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGTA 140 9.528867E-7 11.831868 2 CCCATGT 140 1.3855151E-6 11.478678 1 ATACCAC 135 1.9372237E-9 10.634074 19 GATACCA 130 1.2178134E-8 10.417996 18 ATGTACT 170 1.7127604E-8 10.14379 4 GTACTCT 175 4.503454E-8 9.538871 6 TGTACTC 175 4.503454E-8 9.538871 5 TGATACC 135 4.8716174E-8 9.537287 17 CATGTAC 190 6.8714144E-8 9.270907 3 TACTCTG 165 2.989218E-7 9.242853 7 CTCTGCG 150 3.2721255E-7 9.202564 9 ACTCTGC 160 3.685218E-7 9.118394 8 TGCGTTG 150 3.9498082E-7 9.086076 12 TTGATAC 145 1.1050906E-7 9.041726 16 CTGCGTT 160 8.3323357E-7 8.627403 11 TCTGCGT 160 8.3323357E-7 8.627403 10 TACATGG 280 7.903327E-8 8.380906 2 GTACATG 305 6.148184E-9 8.342427 1 GCGTTGA 165 1.5639725E-6 8.260069 13 GTTGATA 160 4.8168295E-7 8.212814 15 >>END_MODULE