FastQCFastQC Report
Thu 26 May 2016
SRR1926625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23966
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC9403.922223149461738No Hit
GTACATGGGAAGCAGTGGTATCAAC8043.3547525661353585No Hit
GTATCAACGCAGAGTACTTTTTTTT4271.781690728532087No Hit
CATGTACTCTGCGTTGATACCACTG3741.5605441041475423No Hit
GAGTACATGGGAAGCAGTGGTATCA3701.5438537928732372No Hit
GGTATCAACGCAGAGTACTTTTTTT3671.5313360594175083No Hit
ACGCAGAGTACATGGGAAGCAGTGG2991.2476007677543186No Hit
TATCAACGCAGAGTACTTTTTTTTT2751.147458900108487No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2150.897104230993908No Hit
ACTCTGCGTTGATACCACTGCTTCC2140.8929316531753316No Hit
GCGTTGATACCACTGCTTCCCATGT2110.8804139197196028No Hit
GTATCAACGCAGAGTACATGGGAAG1980.8261704080781107No Hit
TATCAACGCAGAGTACATGGGAAGC1880.7844446298923474No Hit
GGTATCAACGCAGAGTACATGGGAA1710.7135108069765501No Hit
CAGTGGTATCAACGCAGAGTACATG1250.5215722273220396No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1120.46732871568054746No Hit
CATGGGAAGCAGTGGTATCAACGCA1080.45063840440624214No Hit
GTGGTATCAACGCAGAGTACATGGG1050.4381206709505132No Hit
ATACCACTGCTTCCCATGTACTCTG1030.4297755153133606No Hit
GATACCACTGCTTCCCATGTACTCT1030.4297755153133606No Hit
CCACTGCTTCCCATGTACTCTGCGT990.41308520403905535No Hit
GTACTCTGCGTTGATACCACTGCTT980.408912626220479No Hit
GCAGTGGTATCAACGCAGAGTACAT950.39639489276475004No Hit
GCAGAGTACATGGGAAGCAGTGGTA950.39639489276475004No Hit
GCTTCCCATGTACTCTGCGTTGATA940.3922223149461737No Hit
GGGAAGCAGTGGTATCAACGCAGAG930.38804973712759744No Hit
CTGCTTCCCATGTACTCTGCGTTGA880.36718684803471585No Hit
ACATGGGAAGCAGTGGTATCAACGC860.3588416923975632No Hit
GTACTTTTTTTTTTTTTTTTTTTTT830.34632395894183426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA790.329633647667529No Hit
TACCACTGCTTCCCATGTACTCTGC750.31294333639322375No Hit
AAGCAGTGGTATCAACGCAGAGTAC750.31294333639322375No Hit
CTTCCCATGTACTCTGCGTTGATAC720.30042560293749476No Hit
ATGGGAAGCAGTGGTATCAACGCAG660.2753901360260369No Hit
GCAGAGTACTTTTTTTTTTTTTTTT610.2545272469331553No Hit
GTTGATACCACTGCTTCCCATGTAC590.24618209129600266No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA560.23366435784027373No Hit
ATCAACGCAGAGTACATGGGAAGCA520.21697404656596847No Hit
GTGGTATCAACGCAGAGTACTTTTT510.21280146874739211No Hit
ATCAACGCAGAGTACTTTTTTTTTT500.2086288909288158No Hit
CCATGTACTCTGCGTTGATACCACT480.2002837352916632No Hit
AAAAAGTACTCTGCGTTGATACCAC390.16273053492447634No Hit
ACCATGTACTCTGCGTTGATACCAC330.13769506801301845No Hit
GTACATGGTAAGCAGTGGTATCAAC330.13769506801301845No Hit
TCCATGTACTCTGCGTTGATACCAC320.13352249019444212No Hit
TTCCCATGTACTCTGCGTTGATACC320.13352249019444212No Hit
CTGCGTTGATACCACTGCTTCCCAT290.12100475673871318No Hit
CAACGCAGAGTACTTTTTTTTTTTT270.11265960110156054No Hit
TCAACGCAGAGTACATGGGAAGCAG260.10848702328298424No Hit
CTCTGCGTTGATACCACTGCTTCCC250.1043144454644079No Hit
GGAAGCAGTGGTATCAACGCAGAGT250.1043144454644079No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA250.1043144454644079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT2451.0913936E-118.91542114
TGCGTTG2552.5465852E-118.56579712
TGATACC2552.5465852E-118.547914517
CCATGTA2459.822543E-118.5456722
CTGCGTT2603.8198777E-118.4010711
GTTGATA2604.0017767E-118.38353215
TTGATAC2604.0017767E-118.38353216
TACTCTG2501.4915713E-108.3747597
CCCATGT2501.5643309E-108.3572391
CTCTGCG2552.237357E-108.2105489
ACTCTGC2552.237357E-108.2105488
TCTGCGT2552.3464963E-108.19337110
GCGTTGA2708.54925E-118.0899213
GGGAAGC2458.241841E-97.76879267
ATGTACT2602.9504008E-97.6866234
TGTACTC2602.9504008E-97.6866235
GATACCA2852.8376235E-107.64813418
ATACCAC2852.8376235E-107.64813419
GAAGCAG2404.8783477E-87.53411059
AGTGGTA2405.0658855E-87.518348714
Produced by FastQC (version 0.11.5)