FastQCFastQC Report
Thu 26 May 2016
SRR1926624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004270
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10760.10714250151851593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA6600.017.0526872
GTCCTAC8250.015.23422051
GTATTAG902.8385202E-614.8560671
CTGTAGG6300.014.4316081
GTGTAGG1406.0572347E-1014.3254941
GTAGGAC7600.014.1518643
CCTTATT903.6482765E-513.7253322
GTCCTAA3150.013.5826891
TCCTACA9500.013.4363782
GTCTTAC700.001439047513.37046051
CCAATAC755.382046E-513.1698983
ATTCCCG657.472303E-512.71737411
CCCTTTA957.748232E-512.6667511
GGTCTAC957.748232E-512.6667511
TAGGACC3150.012.6161344
CCCCTGT750.002417934812.4790961
GACCTAC750.002417934812.4790961
GTCCTAG1401.3406498E-712.4154281
GTGTAAG1307.375511E-712.3419631
CCCAATA800.003222813412.00966552
Produced by FastQC (version 0.11.5)