##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1004270 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.38416162984058 27.0 7.0 31.0 2.0 31.0 2 16.401974568592113 16.0 2.0 30.0 2.0 31.0 3 20.145010803867486 25.0 15.0 31.0 2.0 31.0 4 22.36120565186653 28.0 2.0 35.0 2.0 37.0 5 22.655730032760115 29.0 2.0 35.0 2.0 37.0 6 23.395745168132077 30.0 2.0 35.0 2.0 37.0 7 23.34496002071156 30.0 7.0 35.0 2.0 37.0 8 23.461546197735668 30.0 10.0 35.0 2.0 37.0 9 26.294872892747968 32.0 17.0 37.0 2.0 39.0 10 27.043200533721013 32.0 23.0 37.0 2.0 39.0 11 24.129546835014487 30.0 7.0 37.0 2.0 39.0 12 25.31365469445468 31.0 12.0 37.0 2.0 39.0 13 21.934518605554285 27.0 2.0 37.0 2.0 39.0 14 25.31584733189282 31.0 17.0 38.0 2.0 40.0 15 26.419823354277238 31.0 17.0 38.0 2.0 40.0 16 26.784836747089926 31.0 17.0 39.0 2.0 40.0 17 26.118337698029414 31.0 13.0 38.0 2.0 40.0 18 26.19222918139544 31.0 17.0 38.0 2.0 40.0 19 26.59338624075199 31.0 18.0 39.0 2.0 40.0 20 26.852059705059396 31.0 18.0 39.0 2.0 40.0 21 24.694623955709122 30.0 7.0 38.0 2.0 40.0 22 25.628672568134068 31.0 7.0 38.0 2.0 40.0 23 25.61601262608661 31.0 2.0 38.0 2.0 40.0 24 24.657448694076294 31.0 2.0 39.0 2.0 40.0 25 24.457339161779203 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.107134980439245 1101 2 10.686016151510728 1101 3 7.554007839483365 1101 4 8.95065179352698 1101 5 8.513631478243752 1101 6 8.024960280557483 1101 7 7.701190211392337 1101 8 6.067680250666129 1101 9 5.996035219753836 1101 10 5.68205354678415 1101 11 8.33106533372953 1101 12 5.855777759514513 1101 13 10.589599435673705 1101 14 7.970444069281324 1101 15 7.095877795621234 1101 16 6.597130006245788 1101 17 2.989228506892399 1101 18 5.320256380167752 1101 19 5.835530843666792 1101 20 3.718579292181115 1101 21 1.1013730508333701 1101 22 4.7446455891033175 1101 23 5.982482368496957 1101 24 7.322855352026409 1101 25 7.631153580220666 1102 1 6.142151750202373 1102 2 10.9416700807921 1102 3 7.461929490011599 1102 4 8.772902807691917 1102 5 8.277428373525144 1102 6 7.234365307183442 1102 7 7.121882733660261 1102 8 7.256387266183623 1102 9 6.454568554126208 1102 10 5.677611990569108 1102 11 8.431867212674849 1102 12 5.845285942460958 1102 13 10.338550711371727 1102 14 7.868385178088101 1102 15 5.821564519030769 1102 16 6.132944860200279 1102 17 7.06764709739203 1102 18 6.87065223738065 1102 19 5.6586915823291974 1102 20 6.17157431868171 1102 21 5.120739848701394 1102 22 5.920759442564147 1102 23 6.421745129119472 1102 24 7.761888394507665 1102 25 7.755145471644624 1103 1 5.033787055729675 1103 2 9.301599620906472 1103 3 6.406676418884771 1103 4 7.952291567169979 1103 5 7.231542911065382 1103 6 7.087175674292155 1103 7 6.838687673289009 1103 8 6.246125556588282 1103 9 5.404901839120626 1103 10 4.625322128794743 1103 11 5.48193889286458 1103 12 4.472368107462973 1103 13 8.638837803968471 1103 14 6.7877518098358145 1103 15 5.646272736207038 1103 16 5.584586507252503 1103 17 3.3348838928396525 1103 18 1.9409475002682939 1103 19 4.070554695984132 1103 20 4.607831683183164 1103 21 6.8520505195339965 1103 22 2.379669842834037 1103 23 4.668893555620148 1103 24 6.122353719110247 1103 25 2.2155425471064056 1104 1 5.952752380555051 1104 2 10.582603441609166 1104 3 7.345767393623493 1104 4 7.715945655715746 1104 5 7.7574176681684754 1104 6 7.3054901010554545 1104 7 7.441853210813299 1104 8 7.395000024841 1104 9 6.311473841641497 1104 10 5.354827866413565 1104 11 8.321598049421482 1104 12 5.265932652392337 1104 13 9.800912406142725 1104 14 7.531391521510617 1104 15 6.5900837886727714 1104 16 6.184839907809195 1104 17 6.628983139202763 1104 18 4.558429512767635 1104 19 5.188693843087751 1104 20 4.273233316500743 1104 21 3.7650021764847885 1104 22 5.51105048198114 1104 23 6.003628141513747 1104 24 7.03986461664714 1104 25 5.9800244545692465 1105 1 3.5298696771647826 1105 2 9.417283948851779 1105 3 6.409413816446815 1105 4 5.716517406922851 1105 5 2.365275191142363 1105 6 4.849157356130476 1105 7 5.842280239524165 1105 8 2.954221054382561 1105 9 3.887804899218988 1105 10 3.5699961468766794 1105 11 6.742243394938331 1105 12 4.255496408168256 1105 13 4.9844536293398605 1105 14 3.7348976349003706 1105 15 4.330830310141021 1105 16 4.5906898117601855 1105 17 5.148552800694901 1105 18 2.558690402648743 1105 19 2.5882194388512083 1105 20 1.8532311099694105 1105 21 2.9714679622695357 1105 22 4.266839706320479 1105 23 1.8734398724726802 1105 24 4.740668053644182 1105 25 5.471509839544069 1106 1 6.588434904552795 1106 2 11.27456772732345 1106 3 8.067086563583967 1106 4 9.12412606226572 1106 5 8.793558972516301 1106 6 7.956435894392552 1106 7 7.557254114257681 1106 8 7.385453864479622 1106 9 6.3321330737137735 1106 10 5.674022530445189 1106 11 8.079672583582973 1106 12 5.332625198976185 1106 13 4.818593095019068 1106 14 5.870701939353648 1106 15 6.02307715400309 1106 16 5.970455379294233 1106 17 6.374674132991583 1106 18 6.381001505991801 1106 19 6.153480686678943 1106 20 5.79325731424213 1106 21 5.8990295621426085 1106 22 6.3236951742453975 1106 23 6.711988531124138 1106 24 7.367603182429185 1106 25 6.429770360836894 1107 1 3.830343843969686 1107 2 -13.920237467481744 1107 3 -5.708653338083826 1107 4 1.7201307998399145 1107 5 4.3200701071928655 1107 6 4.639913604381398 1107 7 4.64556717977117 1107 8 5.225030512645876 1107 9 3.376523730776757 1107 10 2.587186513863678 1107 11 5.292884080463612 1107 12 2.666958151283687 1107 13 4.246172938293501 1107 14 3.6354242266104 1107 15 3.30922181528031 1107 16 3.2565139654588613 1107 17 3.858182141420059 1107 18 -1.3356392233742724 1107 19 1.5160497189192341 1107 20 1.825810921822253 1107 21 -1.2896264266194244 1107 22 1.7215687746640675 1107 23 -1.2624333884197334 1107 24 2.166084128842261 1107 25 2.8963215720858777 1108 1 5.367375112966791 1108 2 10.73436965518643 1108 3 -0.006660467520450908 1108 4 5.973152475182317 1108 5 7.534490295230505 1108 6 7.587026922063728 1108 7 7.88326207046693 1108 8 7.306703799444012 1108 9 5.895896859268305 1108 10 4.992612117916725 1108 11 7.515181995153533 1108 12 2.696274011146638 1108 13 3.7339928573478254 1108 14 5.203330919732522 1108 15 5.231662556852019 1108 16 5.42585563318049 1108 17 0.4301326715528333 1108 18 4.2896579931623116 1108 19 5.249423454064676 1108 20 5.609255851060585 1108 21 5.602468772918314 1108 22 2.586015472473971 1108 23 5.361999910361067 1108 24 6.17490881213509 1108 25 6.871719814043011 1109 1 3.6129034095144235 1109 2 -13.920237467481744 1109 3 -5.7888452880355725 1109 4 2.253612729015842 1109 5 2.075743355025377 1109 6 2.5971549997506926 1109 7 3.9202530198723835 1109 8 3.887027578675731 1109 9 3.2635781696355615 1109 10 2.5816490979824636 1109 11 0.8377590762592888 1109 12 1.57614931729103 1109 13 2.4829758939967626 1109 14 3.273554325159715 1109 15 3.270828590836768 1109 16 3.104753454006289 1109 17 3.688574605204675 1109 18 3.8744552897910793 1109 19 2.129325804475254 1109 20 0.9626215099875353 1109 21 0.5016800697700319 1109 22 2.485172021177064 1109 23 2.8965268632704024 1109 24 4.065983742562064 1109 25 4.436214122544214 1110 1 3.3931066980664504 1110 2 9.35940855021737 1110 3 6.659167433848754 1110 4 7.799201890631274 1110 5 8.483166863663246 1110 6 4.04350967734937 1110 7 6.563098853076642 1110 8 7.441656299981787 1110 9 5.655631122413826 1110 10 5.403361320422686 1110 11 3.553308963875761 1110 12 5.932153489077773 1110 13 10.384966442077292 1110 14 7.968628381258544 1110 15 7.075896003743836 1110 16 6.742087159082995 1110 17 7.300947808014779 1110 18 7.31121176001891 1110 19 6.081778450221027 1110 20 6.227507250531314 1110 21 4.0539502858975 1110 22 6.273367792251989 1110 23 6.569843950041502 1110 24 7.570965447969211 1110 25 7.953502598843851 1111 1 5.175720728762585 1111 2 9.544543453206 1111 3 7.47381754992864 1111 4 8.03824498007414 1111 5 8.492216677395522 1111 6 7.4199697561757105 1111 7 7.439489725712711 1111 8 7.423136527135156 1111 9 5.882454656445109 1111 10 5.283362694730009 1111 11 7.175258615145275 1111 12 6.524130479246569 1111 13 8.142788950286324 1111 14 5.956264523577882 1111 15 5.693181844942622 1111 16 5.468708964431997 1111 17 6.6859894544349565 1111 18 6.395081809444893 1111 19 5.259657010535943 1111 20 4.72946220270051 1111 21 7.598894552827247 1111 22 7.039328163118636 1111 23 6.999885326508451 1111 24 7.111930113546247 1111 25 7.754264357760899 1112 1 -2.4989521648994923 1112 2 7.24339889615462 1112 3 5.402585575441673 1112 4 -0.7368952533751632 1112 5 3.6755994863502934 1112 6 2.5486719192372753 1112 7 5.091285028995117 1112 8 5.387045479386455 1112 9 3.9427238416414987 1112 10 2.571873320959021 1112 11 -0.5891974051239721 1112 12 3.4022962887559736 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2204 7 5.390351350100914 2204 8 5.384915628243444 2204 9 3.866690481726561 2204 10 3.446444692569866 2204 11 5.207270564041366 2204 12 5.326771302046776 2204 13 3.1621231982745712 2204 14 4.514169963828536 2204 15 4.77607195985831 2204 16 4.725655963098934 2204 17 -0.6053038092289214 2204 18 3.0959760305773116 2204 19 3.727190653295086 2204 20 4.2080485743423175 2204 21 5.798860496527318 2204 22 5.3037305723585995 2204 23 3.920783245182008 2204 24 5.708112888842781 2204 25 5.8535491754623905 2205 1 6.0644081329947035 2205 2 10.604056317828991 2205 3 7.524584163012296 2205 4 7.791902255613024 2205 5 7.985001771917833 2205 6 7.468728416412414 2205 7 7.503781818928349 2205 8 7.237626501471713 2205 9 6.154816163161787 2205 10 5.462888344071498 2205 11 7.809451156766116 2205 12 6.915194337035377 2205 13 10.222079586420076 2205 14 7.744549585198346 2205 15 6.524203588364607 2205 16 6.065540986391628 2205 17 6.798432933758495 2205 18 6.622361202546781 2205 19 5.380803498968593 2205 20 5.756110820352827 2205 21 -0.6839128209471603 2205 22 4.183877266778264 2205 23 5.45601964125694 2205 24 7.217638114335891 2205 25 7.611354706238483 2206 1 4.103426196176052 2206 2 8.73722732125065 2206 3 6.061405997976884 2206 4 1.901672288238153 2206 5 5.784311074058362 2206 6 6.011629665716146 2206 7 6.208850649993838 2206 8 6.270872303970322 2206 9 5.065318271859169 2206 10 4.214208468206138 2206 11 3.389062838153876 2206 12 4.759898721534462 2206 13 8.013137118050537 2206 14 0.7598054139562045 2206 15 2.981401010183653 2206 16 3.8863956056324973 2206 17 4.901878465707245 2206 18 -0.6572106920979195 2206 19 1.4140747649827574 2206 20 3.020861357473855 2206 21 4.028174391593623 2206 22 -1.1901579047025983 2206 23 -2.1532172490112202 2206 24 1.9353511723449621 2206 25 3.4609719577702727 2207 1 1.6362345934255629 2207 2 -13.920237467481744 2207 3 -6.165424056530725 2207 4 -4.291145018130244 2207 5 1.256652795462113 2207 6 2.1158657087714907 2207 7 3.2095423215875165 2207 8 3.4343659744463118 2207 9 0.9030187472821503 2207 10 0.6667538034724814 2207 11 -0.9653772237240048 2207 12 -2.0723684872385917 2207 13 0.7567615095982134 2207 14 -5.3790216600461775 2207 15 -1.3605909068157551 2207 16 -0.08955106924135592 2207 17 1.1048055162219512 2207 18 1.1507618354191074 2207 19 0.4689286959289838 2207 20 0.35451239538620527 2207 21 -3.5655228117529667 2207 22 -4.220460247800869 2207 23 -1.2437779565572455 2207 24 0.5567800330306412 2207 25 1.2002921110071156 2208 1 4.296049945224134 2208 2 6.220122991283958 2208 3 5.933692211159862 2208 4 7.892650511482589 2208 5 3.164817685545966 2208 6 5.7579291557200705 2208 7 6.213593690431988 2208 8 6.406898949919199 2208 9 4.600323079514496 2208 10 4.0527503255764685 2208 11 6.092515580825086 2208 12 5.606878360152624 2208 13 8.284859950951823 2208 14 2.7381951186507827 2208 15 4.182210234728078 2208 16 4.642169484294971 2208 17 5.33088131953145 2208 18 0.8510180138351089 2208 19 3.098492884843374 2208 20 3.945609936330939 2208 21 6.393011585153673 2208 22 5.416902040990127 2208 23 1.4443184622525393 2208 24 2.7527971919909646 2208 25 4.2586848984399595 2209 1 3.135127774184703 2209 2 4.879876104352441 2209 3 5.207181685606354 2209 4 0.6384012594532926 2209 5 5.187390630328405 2209 6 5.459319278692128 2209 7 6.0584127585909044 2209 8 4.6719136457175665 2209 9 4.443712110187263 2209 10 3.4388861881315975 2209 11 -0.1826325014019119 2209 12 3.1914972850906977 2209 13 2.615135342490877 2209 14 3.7441280219494217 2209 15 4.322303666324931 2209 16 3.8057848770931777 2209 17 1.4204042713074578 2209 18 3.399778694534195 2209 19 3.8659246262171685 2209 20 3.919042128642605 2209 21 6.224935194914398 2209 22 4.9967349458704575 2209 23 0.7888486386486413 2209 24 -2.67009227767943 2209 25 0.35824447418620053 2210 1 -3.19704154654913 2210 2 -13.920237467481744 2210 3 -8.154417611116843 2210 4 -3.0616365100906364 2210 5 -1.6019109160514766 2210 6 -2.261930630009541 2210 7 -2.171638309254117 2210 8 -20.960113611522637 2210 9 -13.674409177425181 2210 10 -8.07849076310292 2210 11 -10.50847147549161 2210 12 -5.715096790354952 2210 13 -5.995022756608774 2210 14 -5.086444647245305 2210 15 -6.87047991616237 2210 16 -6.397117850539061 2210 17 -5.760674371594664 2210 18 -6.022648650089206 2210 19 -11.397352454183924 2210 20 -8.3462086407309 2210 21 -6.241967432700928 2210 22 -10.69513236327326 2210 23 -8.870237469989465 2210 24 -11.206393152235194 2210 25 -8.990743479698107 2211 1 -0.1701423146569283 2211 2 4.723614040638907 2211 3 0.510372698411512 2211 4 1.259451772568731 2211 5 0.7460685329649337 2211 6 -0.3703536028044887 2211 7 -20.796783152823064 2211 8 -13.250716859782498 2211 9 -9.22354777826991 2211 10 -7.134128841032123 2211 11 -9.6417430178639 2211 12 -4.973101834002939 2211 13 -6.692403877341771 2211 14 -4.974782017827071 2211 15 -5.398205865408649 2211 16 -12.324108626255349 2211 17 -8.095456588702739 2211 18 -6.225893414884759 2211 19 -8.819265975515922 2211 20 -6.8779821443724884 2211 21 -4.722186707716858 2211 22 -6.198608608927952 2211 23 -8.035721415541726 2211 24 -22.19422629244377 2211 25 -21.974520999976207 2212 1 -1.4473435462454205 2212 2 -13.920237467481744 2212 3 -8.153487328926685 2212 4 -19.83973616246607 2212 5 -20.06019596819516 2212 6 -20.801328080762726 2212 7 -10.197019280331919 2212 8 -9.764718097945305 2212 9 -5.732519664852006 2212 10 -6.181631696750543 2212 11 -6.186253190247939 2212 12 -3.4080195317871222 2212 13 -6.709049223137084 2212 14 -4.371508419457502 2212 15 -4.427028076734551 2212 16 -4.407810623477701 2212 17 -4.684306412154967 2212 18 -3.693429991364596 2212 19 -4.321828589025593 2212 20 -4.4792902962501415 2212 21 -2.361429346537644 2212 22 -3.561064335020042 2212 23 -3.694150784106089 2212 24 -2.6104010268003215 2212 25 -3.205925958653893 2213 1 -18.884179437626766 2213 2 -13.920237467481744 2213 3 -8.561566150985948 2213 4 -5.599364316516535 2213 5 -20.06019596819516 2213 6 -10.59150072352501 2213 7 -20.796783152823064 2213 8 -10.567019322014005 2213 9 -8.368728077952849 2213 10 -7.124443683749419 2213 11 -6.563328909409858 2213 12 -5.000008129932908 2213 13 -19.352496684766365 2213 14 -12.375487779467287 2213 15 -7.025824547678795 2213 16 -6.16772256955408 2213 17 -4.782652892428285 2213 18 -4.772540546389674 2213 19 -5.6428240248345 2213 20 -5.709108528241501 2213 21 -3.8135366983190266 2213 22 -4.834730786882798 2213 23 -4.62214678906696 2213 24 -3.582008496959041 2213 25 -4.581426710467575 2214 1 -18.884179437626766 2214 2 -13.920237467481744 2214 3 -12.72778723157587 2214 4 -19.83973616246607 2214 5 -20.06019596819516 2214 6 -20.801328080762726 2214 7 -16.811403035864 2214 8 -15.335113611522637 2214 9 -14.590327062133088 2214 10 -14.494929576435982 2214 11 -14.940536453502494 2214 12 -13.015772557602348 2214 13 -19.352496684766365 2214 14 -17.281151673598366 2214 15 -23.970711665872685 2214 16 -18.794519475497555 2214 17 -23.6784032244336 2214 18 -18.33297133784374 2214 19 -15.770031568927136 2214 20 -14.375577156651834 2214 21 -12.026870497624728 2214 22 -12.990275275594618 2214 23 -13.003778158326764 2214 24 -11.890863719344354 2214 25 -11.595866029215973 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 177806.0 3 4.0 4 1012.0 5 3528.0 6 4668.0 7 4257.0 8 3778.0 9 3553.0 10 3304.0 11 3038.0 12 2924.0 13 3147.0 14 4743.0 15 10252.0 16 15081.0 17 15980.0 18 16686.0 19 19133.0 20 22788.0 21 26161.0 22 27531.0 23 29525.0 24 31016.0 25 32066.0 26 26216.0 27 20706.0 28 22158.0 29 25943.0 30 25003.0 31 28620.0 32 37065.0 33 47131.0 34 57472.0 35 66058.0 36 65847.0 37 69018.0 38 51052.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11917644900954 19.47909024211299 12.786821349963315 25.61491195891416 2 13.816375707526449 20.49097001719399 42.122296966578396 23.570357308701166 3 18.31728785078493 25.0022429547472 29.90887798431726 26.771591210150607 4 10.975514219816732 17.16354805337241 40.38448688615621 31.476450840654646 5 12.702121607506642 37.92947782636284 36.74735216519587 12.621048400934646 6 30.322997234988602 38.375158801330194 18.59528546874416 12.70655849493704 7 26.78826007780631 31.38283033084157 23.77441881410179 18.054490777250333 8 23.615064676552468 38.431138875125626 21.559210647046186 16.394585801275717 9 25.802010833700507 15.709762890688081 20.940303000699785 37.54792327491163 10 13.573991253270432 30.13683552573782 35.78939183874189 20.499781382249854 11 32.58894920536387 22.708519402972467 25.94808419532403 18.754447196339626 12 22.939673022741154 25.149865455999205 32.90384033197717 19.006621189282473 13 27.78601365759244 22.140930794099578 26.1149865867167 23.958068961591284 14 19.36676267607343 23.30510360116182 28.695519381224766 28.63261434153998 15 21.6404838264973 32.77708250695186 23.925447834701156 21.65698583184969 16 20.57854224509605 29.068500403233255 29.377338085945116 20.975619265725584 17 20.341501150120134 29.226063925162784 29.808040082583236 20.624394842133846 18 20.092212835226395 28.285675888960633 31.832626529933805 19.789484745879168 19 22.086903131728086 27.721832422454085 28.147279682343484 22.04398476347435 20 22.297214982574918 28.536592672478356 29.202939796091588 19.963252548855138 21 21.21933367275438 27.03436586048962 27.660212460917123 24.086088005838874 22 21.33317958506174 29.329505810572016 28.811920346257292 20.525394258108957 23 20.783578407184446 28.88801166705045 29.256544523494092 21.07186540227101 24 21.6594005210572 27.871187466556723 29.683045465311096 20.786366547074977 25 21.41772676839523 28.208093292275056 29.39847098124166 20.975708958088056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1053.0 1 1053.0 2 2280.5 3 3508.0 4 3508.0 5 3508.0 6 4941.0 7 6374.0 8 6374.0 9 6374.0 10 7651.5 11 8929.0 12 8929.0 13 8929.0 14 11579.5 15 14230.0 16 14230.0 17 14230.0 18 20146.5 19 26063.0 20 26063.0 21 26063.0 22 36303.5 23 46544.0 24 46544.0 25 46544.0 26 59856.0 27 73168.0 28 73168.0 29 73168.0 30 86975.0 31 100782.0 32 100782.0 33 100782.0 34 109675.5 35 118569.0 36 118569.0 37 118569.0 38 121877.0 39 125185.0 40 125185.0 41 125185.0 42 124540.0 43 123895.0 44 123895.0 45 123895.0 46 119674.0 47 115453.0 48 115453.0 49 115453.0 50 104482.5 51 93512.0 52 93512.0 53 93512.0 54 78952.0 55 64392.0 56 64392.0 57 64392.0 58 52604.5 59 40817.0 60 40817.0 61 40817.0 62 31862.0 63 22907.0 64 22907.0 65 22907.0 66 17205.5 67 11504.0 68 11504.0 69 11504.0 70 8151.5 71 4799.0 72 4799.0 73 4799.0 74 3277.0 75 1755.0 76 1755.0 77 1755.0 78 1169.0 79 583.0 80 583.0 81 583.0 82 381.5 83 180.0 84 180.0 85 180.0 86 117.0 87 54.0 88 54.0 89 54.0 90 33.5 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.138896910193473 2 36.527826182202 3 5.661724436655481 4 13.567666065898612 5 13.534109353062423 6 14.722534776504325 7 9.340515996694117 8 7.755981957043424 9 0.10993059635357025 10 1.3897657004590398 11 5.680145777529947 12 5.601382098439663 13 16.03911298754319 14 1.541218994891812 15 2.850926543658578 16 2.9521941310603723 17 2.8164736574825495 18 1.4540910312963646 19 1.3958397642068368 20 0.282792476126938 21 4.362472243520169 22 1.5574496898244496 23 0.1098310215380326 24 9.188564828183656 25 0.11381401415953878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1004270.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.75812275326827 #Duplication Level Percentage of deduplicated Percentage of total 1 96.80128250105302 89.79105244906475 2 2.412136728254387 4.474905494741747 3 0.3765386292971033 1.0478104919306426 4 0.14375662428970365 0.5333837840983919 5 0.0771911289722289 0.35800521083346853 6 0.03959628882173058 0.22037264514599697 7 0.02750795129032978 0.17861101457355338 8 0.01833877777276874 0.1360856479833112 9 0.013778175008866654 0.11502338839096196 >10 0.07788358026668737 1.3450739321029253 >50 0.0054427559306652225 0.34726170053978234 >100 0.005881077399713252 0.9961354852653629 >500 5.548180357317905E-4 0.34552848672221315 >1k 1.1096360714635809E-4 0.11075026860682197 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1076 0.10714250151851593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 660 0.0 17.052687 2 GTCCTAC 825 0.0 15.2342205 1 GTATTAG 90 2.8385202E-6 14.856067 1 CTGTAGG 630 0.0 14.431608 1 GTGTAGG 140 6.0572347E-10 14.325494 1 GTAGGAC 760 0.0 14.151864 3 CCTTATT 90 3.6482765E-5 13.725332 2 GTCCTAA 315 0.0 13.582689 1 TCCTACA 950 0.0 13.436378 2 GTCTTAC 70 0.0014390475 13.3704605 1 CCAATAC 75 5.382046E-5 13.169898 3 ATTCCCG 65 7.472303E-5 12.717374 11 CCCTTTA 95 7.748232E-5 12.666751 1 GGTCTAC 95 7.748232E-5 12.666751 1 TAGGACC 315 0.0 12.616134 4 CCCCTGT 75 0.0024179348 12.479096 1 GACCTAC 75 0.0024179348 12.479096 1 GTCCTAG 140 1.3406498E-7 12.415428 1 GTGTAAG 130 7.375511E-7 12.341963 1 CCCAATA 80 0.0032228134 12.0096655 2 >>END_MODULE