FastQCFastQC Report
Thu 26 May 2016
SRR1926624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004270
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22560.22464078385294792No Hit
GTCCTACAGTGGACATTTCTAAATT19790.19705855994901772No Hit
GTCCTAAAGTGTGTATTTCTCATTT17660.17584912423949736No Hit
GTATCAACGCAGAGTACTTTTTTTT17500.1742559271908949No Hit
CTTTAGGACGTGAAATATGGCGAGG17010.16937676122954984No Hit
GTCCTACAGTGTGCATTTCTCATTT15770.1570294841028807No Hit
CTGTAGGACCTGGAATATGGCGAGA14060.14000219064594183No Hit
CCCATGTACTCTGCGTTGATACCAC11010.10963187190695729No Hit
GTACATGGGAAGCAGTGGTATCAAC10380.10335865852808507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.013.8391084
GGCGAGG5600.012.5176519
GGGTTAC802.7666529E-511.91517451
TGTAGGA14550.011.8673632
GTCTAAG657.794982E-411.7318641
GTAGGAC13600.011.7119473
CTGTAGG14850.011.4899191
GGACGTG11450.011.4092686
ACTGAGC1604.5474735E-1111.2860558
TTAGGAC7700.011.2720333
AGGACGT11950.011.2510725
TGGCGAG13400.011.16999518
ATAGGAC1201.204844E-711.127523
AGGACCT9500.011.0410865
GACGTGG5000.011.0235927
TAGGACG12600.010.9733734
GGACCTG9700.010.9117386
TGGCAAG9050.010.78175318
GACGTGA7000.010.7249247
GTACTAA803.652335E-410.7236571
Produced by FastQC (version 0.11.5)