FastQCFastQC Report
Thu 26 May 2016
SRR1926623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035041
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA6550.014.8896182
GTCCTAC6750.014.2748071
ATCCTAC852.7028373E-514.1698471
GTCCTAA2200.013.9909331
TAGGACC3500.013.8469334
TCTAGTA903.6215737E-513.7361972
GTAGGAC7300.013.5596933
CTGTAGG6650.013.48325351
GCCCTAC700.00142929513.3826331
GTCTAAG904.6279936E-513.3826311
TCCTACA8100.012.8882852
GTCTAGG854.2842905E-412.5954191
TAGGACA2400.012.564814
GTATAAG750.002401618312.4904581
TCTAGGA1654.7184585E-912.48745252
GTCCTAG1307.295912E-712.3531981
TAAAGGT1057.632443E-712.3083854
CCTACAG9300.012.2993073
GTATTAT1102.2312332E-512.1660291
GTGTATA3900.012.0100551
Produced by FastQC (version 0.11.5)