FastQCFastQC Report
Thu 26 May 2016
SRR1926623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035041
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18370.17748089206128065No Hit
CTGTAGGACGTGGAATATGGCAAGA17390.1680126680972058No Hit
GTCCTACAGTGTGCATTTCTCATTT16720.16153949457074646No Hit
GTATCAACGCAGAGTACTTTTTTTT16370.1581579860121483No Hit
CTGTAGGACCTGGAATATGGCGAGA14420.13931815261424427No Hit
CTTTAGGACGTGAAATATGGCGAGG13870.1340043534507329No Hit
GTCCTAAAGTGTGTATTTCTCATTT13350.12898039787795848No Hit
GTACATGGGAAGCAGTGGTATCAAC11450.11062363713128273No Hit
CCCATGTACTCTGCGTTGATACCAC11270.10888457558686081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT653.4750938E-614.57197919
TAGGACC5300.014.3985974
TCCAACG1203.6379788E-1214.20767918
GCCGGTT551.9936168E-413.78980111
TGGCGAA500.001532927513.26050118
GTAGGAC12100.012.772563
TGTAGGA12950.012.6727352
AGGACCT8150.012.4066435
CTGTAGG13050.012.0573731
TAGGACA4050.012.0121624
GGACCTG7900.011.7108196
CCGGTTT658.158934E-411.66829312
CCTACAC1751.8189894E-1211.4479983
GTATTAG752.0004089E-411.4435431
CCAACGA752.1305797E-411.36614319
TGGCGAG10150.011.29148918
GTCCTAC10750.011.1774141
CCCTTAC951.3026178E-511.0420151
ATAGGGC700.001439309810.9028553
CGGTTTC700.001516455210.83484513
Produced by FastQC (version 0.11.5)