FastQCFastQC Report
Thu 26 May 2016
SRR1926622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798106
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC452.2843796E-417.0397573
ACTGCGT350.00179302416.7552958
ATAAGGG450.004477616414.6055063
CTGTAGG4350.014.4023561
GTCCTAA3100.014.1898291
TGTAGGA4700.013.6845982
GATAATG600.006893234313.6845972
ATACACT1850.013.3393094
GTATTGG600.00820150413.329841
GTATGGA700.001471626313.329841
CTTTATA1451.0963049E-813.2127162
TCTATAC600.00202182412.7798173
GTCCTAC5700.012.628271
GTGTTAG957.9650825E-512.6282691
GTCTTAA957.9650825E-512.6282691
TCCTAAA3400.012.4771342
AGTGCAA605.3991075E-412.2547610
GTAGGAC4750.011.9918893
TAGGACA1803.6379788E-1211.9216054
TATAGGA1152.795526E-511.899652
Produced by FastQC (version 0.11.5)