##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926622_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 798106 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.184652163998265 27.0 7.0 31.0 2.0 31.0 2 16.269758653612428 16.0 2.0 30.0 2.0 31.0 3 19.96523268838976 24.0 15.0 31.0 2.0 31.0 4 22.14804800364864 28.0 2.0 35.0 2.0 37.0 5 22.446761457751226 28.0 2.0 35.0 2.0 37.0 6 23.154554658153177 30.0 2.0 35.0 2.0 37.0 7 23.111567886972406 30.0 2.0 35.0 2.0 37.0 8 23.206961230713716 30.0 7.0 35.0 2.0 37.0 9 26.007729549708937 31.0 17.0 37.0 2.0 39.0 10 26.75372945448349 32.0 23.0 37.0 2.0 39.0 11 23.86110867478756 30.0 7.0 37.0 2.0 39.0 12 25.00211375431334 31.0 10.0 37.0 2.0 39.0 13 21.66972682826592 27.0 2.0 35.0 2.0 39.0 14 25.013260143389473 30.0 16.0 38.0 2.0 40.0 15 26.097880482041234 31.0 17.0 38.0 2.0 40.0 16 26.464395706835933 31.0 17.0 38.0 2.0 40.0 17 25.7816167276026 31.0 10.0 38.0 2.0 40.0 18 25.854251189691595 31.0 11.0 38.0 2.0 40.0 19 26.255016250974183 31.0 16.0 39.0 2.0 40.0 20 26.50917923183136 31.0 13.0 39.0 2.0 40.0 21 24.35447296474403 30.0 2.0 37.0 2.0 40.0 22 25.28128594447354 31.0 2.0 38.0 2.0 40.0 23 25.273025137011874 31.0 2.0 38.0 2.0 40.0 24 24.313612477540577 31.0 2.0 38.0 2.0 40.0 25 24.109724773401027 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.169425009246272 1101 2 10.828736361450655 1101 3 7.56717250576364 1101 4 9.00652481082047 1101 5 8.71534426725417 1101 6 8.070017430570505 1101 7 7.796589229359601 1101 8 6.290181754162507 1101 9 6.257443743930267 1101 10 5.948091047703372 1101 11 8.484878734220473 1101 12 6.386446333786633 1101 13 10.82039028399884 1101 14 8.115484460420646 1101 15 7.512305699826122 1101 16 6.900849072597104 1101 17 2.5364269981224226 1101 18 5.268207000358572 1101 19 5.989782253762385 1101 20 4.17784778905607 1101 21 1.7296529550708009 1101 22 5.305587838075237 1101 23 6.467624265602566 1101 24 7.63939722244498 1101 25 7.9623378238942415 1102 1 5.9118491956514525 1102 2 10.627048827284028 1102 3 7.082235926942062 1102 4 8.10312095669314 1102 5 7.960839026391017 1102 6 6.84749584782719 1102 7 6.7060634528694045 1102 8 7.069249992339866 1102 9 5.940432924168952 1102 10 5.418171515881863 1102 11 7.900594193248253 1102 12 5.280002805440546 1102 13 9.736500557358248 1102 14 7.263059455336915 1102 15 5.270170691810993 1102 16 5.70393364209103 1102 17 6.527476464792965 1102 18 6.49318250602462 1102 19 5.38678219302172 1102 20 5.808941200278305 1102 21 4.977077682860184 1102 22 5.813393541887901 1102 23 6.136814750973308 1102 24 7.633834433761759 1102 25 7.453636327297037 1103 1 4.9722777184355635 1103 2 9.305892979044504 1103 3 6.520628105719684 1103 4 8.26066089073063 1103 5 7.269351969890593 1103 6 6.834551533631458 1103 7 6.776691019941158 1103 8 6.208414811176489 1103 9 5.544606820589458 1103 10 4.677563950450214 1103 11 5.532505362722372 1103 12 4.222158800854778 1103 13 8.171056961385947 1103 14 6.616347723883507 1103 15 5.568152226001416 1103 16 5.534398154793404 1103 17 3.0592784208588704 1103 18 1.9240915840972725 1103 19 4.0195009226053 1103 20 4.504449221842929 1103 21 6.888389443965767 1103 22 2.5012685726398125 1103 23 4.600667611023194 1103 24 6.041568111625107 1103 25 1.684972157712675 1104 1 5.792813742494772 1104 2 10.408366850149953 1104 3 7.134663389786642 1104 4 7.852246456698129 1104 5 7.680697593970269 1104 6 7.46355240819198 1104 7 7.550517198239586 1104 8 7.2151283022718005 1104 9 6.050649069163963 1104 10 5.104157092211626 1104 11 7.9368902098554805 1104 12 4.909141812210208 1104 13 9.333089416588034 1104 14 6.870853362693744 1104 15 6.384603237022159 1104 16 5.811093435204366 1104 17 6.250708047515857 1104 18 3.8779887611480817 1104 19 4.770447830226448 1104 20 3.9983137081305564 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19 4.3212986224616365 2208 20 5.21135649397592 2208 21 7.795623063866898 2208 22 6.986375408460027 2208 23 2.296495275077561 2208 24 3.5443022876856745 2208 25 5.214123780203305 2209 1 2.818766125183668 2209 2 4.090712332485953 2209 3 4.687945037502725 2209 4 0.40369174034213984 2209 5 4.7848144877903955 2209 6 5.0883084713191415 2209 7 5.717076201007934 2209 8 4.7390974117628595 2209 9 4.093660622889466 2209 10 3.0949705832621497 2209 11 -0.286852388685638 2209 12 2.990963422855181 2209 13 2.2681107809257917 2209 14 3.5633616224249103 2209 15 3.733480111546136 2209 16 3.329115329873648 2209 17 1.0205566686017988 2209 18 2.8784227161188696 2209 19 3.5672251024469723 2209 20 3.7473903165510514 2209 21 5.817284273415286 2209 22 4.563820090122622 2209 23 0.21091098365258176 2209 24 -2.9424133857194192 2209 25 0.0037922416922313573 2210 1 -2.747323673302052 2210 2 -13.743104793784003 2210 3 -7.975175703189217 2210 4 -2.6862565582721416 2210 5 -1.5693378793595691 2210 6 -1.9018612528763832 2210 7 -1.9386973328975898 2210 8 -20.653500556306806 2210 9 -13.373299790381619 2210 10 -7.835364296366908 2210 11 -10.374508868783899 2210 12 -5.397336721432698 2210 13 -5.89910165800686 2210 14 -4.8388849334723005 2210 15 -6.464185782326837 2210 16 -5.8585247876816595 2210 17 -5.367203724269231 2210 18 -5.440988997336877 2210 19 -10.755813995530659 2210 20 -8.03740399353401 2210 21 -6.2909114480984805 2210 22 -10.504594464465583 2210 23 -8.772416222581334 2210 24 -11.578126866967306 2210 25 -9.322293051781664 2211 1 -0.3285488240180747 2211 2 4.4949213310345755 2211 3 0.30115258467253625 2211 4 1.1213985618225486 2211 5 0.738370365874573 2211 6 -0.42693100618448554 2211 7 -20.550703189412964 2211 8 -13.263805345856879 2211 9 -9.436092642712858 2211 10 -7.670631738943946 2211 11 -9.706300864737889 2211 12 -5.286975619234802 2211 13 -7.375575721251275 2211 14 -5.1137065923791525 2211 15 -5.6758087128370285 2211 16 -11.731842580140865 2211 17 -7.903245943880059 2211 18 -6.178906746870972 2211 19 -8.78473425894635 2211 20 -6.931854966467814 2211 21 -4.727708469690665 2211 22 -6.422391811183619 2211 23 -8.439306723168897 2211 24 -21.827028770334802 2211 25 -21.626831851196012 2212 1 -1.3095388303032145 2212 2 -13.743104793784003 2212 3 -7.850564236205495 2212 4 -19.60719360073188 2212 5 -19.817507718305396 2212 6 -20.530704591090146 2212 7 -9.822433808050395 2212 8 -9.446766022242594 2212 9 -5.215635930057445 2212 10 -5.890123517684259 2212 11 -5.412773423222088 2212 12 -2.9933890769373974 2212 13 -5.8515467073137195 2212 14 -3.8113674516363254 2212 15 -3.889410303102675 2212 16 -4.046762799768462 2212 17 -4.282220045235626 2212 18 -3.3784264393650574 2212 19 -3.782971464479548 2212 20 -3.9410025172518672 2212 21 -1.958383366520021 2212 22 -3.2566499202086483 2212 23 -3.3039494532613105 2212 24 -2.268688911290166 2212 25 -2.756040934986146 2213 1 -18.658011813858423 2213 2 -13.743104793784003 2213 3 -8.598336648769308 2213 4 -5.6157187499219905 2213 5 -19.817507718305396 2213 6 -10.523884471738057 2213 7 -20.550703189412964 2213 8 -10.5222132587791 2213 9 -8.22361165107344 2213 10 -7.2297435644023444 2213 11 -6.1577279301687184 2213 12 -5.06925889917769 2213 13 -19.0308732539073 2213 14 -12.216625806382671 2213 15 -6.999567647849162 2213 16 -6.177339779462319 2213 17 -4.809973511539923 2213 18 -4.748236996797658 2213 19 -5.6674039545774875 2213 20 -5.526831809984927 2213 21 -3.815924585315937 2213 22 -4.677734877292501 2213 23 -4.392464802201609 2213 24 -3.3888361019631077 2213 25 -4.304581211809822 2214 1 -18.658011813858423 2214 2 -13.743104793784003 2214 3 -12.431994492754454 2214 4 -19.60719360073188 2214 5 -19.817507718305396 2214 6 -20.530704591090146 2214 7 -16.364446408219475 2214 8 -14.926556614172991 2214 9 -14.043174478956384 2214 10 -13.98789448575052 2214 11 -14.450576054558814 2214 12 -12.441653756955693 2214 13 -19.0308732539073 2214 14 -16.82358174230685 2214 15 -23.598885635913952 2214 16 -18.29882778027629 2214 17 -23.339385276245807 2214 18 -17.829990556779713 2214 19 -15.087222074450645 2214 20 -13.846634359513269 2214 21 -11.479606678956918 2214 22 -12.464176036034479 2214 23 -12.445899604851341 2214 24 -11.276395858942397 2214 25 -11.083432212859666 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 146483.0 3 2.0 4 857.0 5 2851.0 6 3867.0 7 3676.0 8 3026.0 9 2813.0 10 2787.0 11 2476.0 12 2430.0 13 2565.0 14 3893.0 15 7892.0 16 11856.0 17 12721.0 18 13248.0 19 15390.0 20 18064.0 21 20875.0 22 21847.0 23 23803.0 24 24582.0 25 25281.0 26 21342.0 27 16641.0 28 17696.0 29 20819.0 30 20369.0 31 23123.0 32 29822.0 33 37102.0 34 45617.0 35 51513.0 36 51064.0 37 52591.0 38 37122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42850425094265 19.332629410695585 12.786004633195722 25.45286170516604 2 14.249894045870745 20.630402428567205 41.103499935934714 24.016203589627338 3 18.504637672311855 25.059886954663753 29.785017445186018 26.650457927838378 4 11.46010174450213 17.11389498192868 39.735591873777665 31.690411399791525 5 13.1550063422743 37.562481537900155 36.086237554372694 13.196274565452851 6 30.675613910554016 37.56975337762866 18.46471291074948 13.289919801067848 7 26.66722903595614 31.52609969701202 23.688100772889005 18.118570494142833 8 24.073005289352718 37.40556909768667 21.45671082382178 17.064714789138833 9 25.86584281304368 15.796339571189707 20.670597783071113 37.667219832695494 10 14.330747279052453 29.57618526076118 34.98339351648323 21.10967394370313 11 32.518600622564996 22.765038130446612 25.535385983659324 19.180975263329064 12 23.208230594897085 25.090686746907874 31.952174288695563 19.74890836949948 13 27.690498708369375 21.99537134567143 25.732060976320355 24.582068969638836 14 20.09205225371505 22.732096638323974 28.2085313122727 28.96731979568828 15 22.061699284354344 31.71969083282167 23.935560933073972 22.283048949750015 16 21.334805671943695 28.5627670333753 28.305021775475243 21.79740551920576 17 20.867210419420353 28.779780134756116 29.028788806860312 21.324220638963215 18 20.82612371514115 27.823002549058245 30.799107511775887 20.551766224024714 19 22.43737991887853 27.279762328328474 27.816505880798204 22.466351871994796 20 22.694782391277307 28.085025650175083 28.508570789583125 20.71162116896448 21 21.6599654392219 26.65477084160342 27.3597425890189 24.325521130155785 22 22.11112072783924 28.545645234373808 28.318322752103942 21.02491128568301 23 21.520398883627585 28.166954122111072 28.60886199002791 21.703785004233435 24 22.062327278951567 27.544952335202378 28.926996800260618 21.465723585585437 25 21.92636728549925 27.738334169593575 28.691169091821372 21.6441294530858 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 935.0 1 935.0 2 1860.5 3 2786.0 4 2786.0 5 2786.0 6 3994.5 7 5203.0 8 5203.0 9 5203.0 10 6200.0 11 7197.0 12 7197.0 13 7197.0 14 9111.0 15 11025.0 16 11025.0 17 11025.0 18 15510.5 19 19996.0 20 19996.0 21 19996.0 22 27492.0 23 34988.0 24 34988.0 25 34988.0 26 44140.5 27 53293.0 28 53293.0 29 53293.0 30 63839.5 31 74386.0 32 74386.0 33 74386.0 34 81407.0 35 88428.0 36 88428.0 37 88428.0 38 91991.5 39 95555.0 40 95555.0 41 95555.0 42 96111.0 43 96667.0 44 96667.0 45 96667.0 46 94384.0 47 92101.0 48 92101.0 49 92101.0 50 85203.0 51 78305.0 52 78305.0 53 78305.0 54 67566.5 55 56828.0 56 56828.0 57 56828.0 58 47476.5 59 38125.0 60 38125.0 61 38125.0 62 30295.5 63 22466.0 64 22466.0 65 22466.0 66 17186.5 67 11907.0 68 11907.0 69 11907.0 70 8492.5 71 5078.0 72 5078.0 73 5078.0 74 3475.5 75 1873.0 76 1873.0 77 1873.0 78 1284.0 79 695.0 80 695.0 81 695.0 82 446.0 83 197.0 84 197.0 85 197.0 86 125.5 87 54.0 88 54.0 89 54.0 90 34.0 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.137102089196173 2 36.43764111534057 3 5.588606024763628 4 13.574512658719517 5 13.469639371211342 6 14.744031494563378 7 9.394115568608681 8 7.733433904769542 9 0.10988515309996418 10 1.3862820226887154 11 5.60965586024914 12 5.737458432839748 13 16.13783131563978 14 1.534633244205657 15 2.864531778986751 16 2.8720495773744337 17 2.835588255194172 18 1.4460485198707942 19 1.3955539740335243 20 0.27627909074734436 21 4.361951921173378 22 1.5584396057666525 23 0.11038633965914302 24 9.23085404695617 25 0.11351875565401087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 798106.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.99944109050952 #Duplication Level Percentage of deduplicated Percentage of total 1 96.72891986084953 89.9573548434769 2 2.4708827396441424 4.595814275741844 3 0.39296661963753804 1.0963702798055364 4 0.13946167100369883 0.5187942982757004 5 0.07437422699799871 0.3458380771176282 6 0.04163175677548181 0.23230380670415046 7 0.028700249548063706 0.18683750169896507 8 0.019108928850538262 0.14216957623506965 9 0.017601249992430287 0.14732157706113164 >10 0.0746136243366059 1.2591203804797109 >50 0.005939026192119621 0.38918606958563473 >100 0.005247660853665411 0.824513023120049 >500 5.523853181669734E-4 0.30437629069758093 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2529663979471398E-4 0.0 8 0.0 0.0 0.0 1.2529663979471398E-4 0.0 9 0.0 0.0 0.0 1.2529663979471398E-4 0.0 10 0.0 0.0 0.0 1.2529663979471398E-4 0.0 11 0.0 0.0 0.0 1.2529663979471398E-4 0.0 12 0.0 0.0 0.0 1.2529663979471398E-4 1.2529663979471398E-4 13 0.0 0.0 0.0 1.2529663979471398E-4 1.2529663979471398E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAC 45 2.2843796E-4 17.039757 3 ACTGCGT 35 0.001793024 16.755295 8 ATAAGGG 45 0.0044776164 14.605506 3 CTGTAGG 435 0.0 14.402356 1 GTCCTAA 310 0.0 14.189829 1 TGTAGGA 470 0.0 13.684598 2 GATAATG 60 0.0068932343 13.684597 2 ATACACT 185 0.0 13.339309 4 GTATTGG 60 0.008201504 13.32984 1 GTATGGA 70 0.0014716263 13.32984 1 CTTTATA 145 1.0963049E-8 13.212716 2 TCTATAC 60 0.002021824 12.779817 3 GTCCTAC 570 0.0 12.62827 1 GTGTTAG 95 7.9650825E-5 12.628269 1 GTCTTAA 95 7.9650825E-5 12.628269 1 TCCTAAA 340 0.0 12.477134 2 AGTGCAA 60 5.3991075E-4 12.25476 10 GTAGGAC 475 0.0 11.991889 3 TAGGACA 180 3.6379788E-12 11.921605 4 TATAGGA 115 2.795526E-5 11.89965 2 >>END_MODULE