FastQCFastQC Report
Thu 26 May 2016
SRR1926622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798106
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17690.22164975579684904No Hit
CTGTAGGACGTGGAATATGGCAAGA16270.20385763294599965No Hit
GTCCTACAGTGGACATTTCTAAATT14290.17904889826664627No Hit
CTTTAGGACGTGAAATATGGCGAGG13130.16451448805045946No Hit
GTCCTAAAGTGTGTATTTCTCATTT11560.14484291560268936No Hit
GGTATCAACGCAGAGTACTTTTTTT11330.14196109288741096No Hit
GTACATGGGAAGCAGTGGTATCAAC8220.10299383791125488No Hit
TATCAACGCAGAGTACTTTTTTTTT8190.10261794799187075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG3600.013.15598219
CTAATAC1001.3603494E-712.4033673
GTCTATT550.002978558412.1377951
CCCTTAC550.002978558412.1377951
GGTTAGG550.002978558412.1377951
CGGTTTC550.003113433712.06624813
GTCTAGA959.902378E-712.0465331
TAGGACC3900.011.9859624
CTTACAC1052.565539E-711.8127313
GTATTAC657.7597186E-411.7376481
CCCTTAT657.7597186E-411.7376481
TGTAGGA9300.011.2872512
GGACCTG5950.011.223356
TTTATAC700.001437354410.9040583
GTCCTAC7650.010.8458171
ACGAAAT700.00151561610.83499812
TAACTCC803.6233733E-410.7323284
GTTTTAG803.633956E-410.7289441
GTATAAG1252.1173582E-710.6812581
TGGCGAG9050.010.6759718
Produced by FastQC (version 0.11.5)