FastQCFastQC Report
Thu 26 May 2016
SRR1926621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714532
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG404.5931397E-420.518292
GGCCAGA450.001190921517.8086781
GTCCTAC1655.14774E-1012.9517651
TGACAGT653.6750565E-412.8259836
TCTACAC809.923133E-512.3352893
TAGGACA705.316482E-412.2761884
GTTTAGA1001.2616378E-412.0208581
GCACTGT801.5945491E-411.723756
TCTACTC757.9870655E-411.6956813
TTCTAGA959.0670784E-411.519042
GTGTAGA1051.9868038E-411.4484361
TCCAACG600.001012841511.36977818
GTATAGC950.001107181711.2475851
TAGGCAG650.005322364611.229225
GTCCTAA1452.778328E-611.0536611
GTCCTGT850.00620384410.9994761
TCTATAC1006.445988E-510.9647033
TATAAAC700.00635502210.9647023
GTATCAA5500.010.9280521
TTAAGGG700.00740397310.7416654
Produced by FastQC (version 0.11.5)