##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926621_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714532 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.87721193732401 27.0 2.0 31.0 2.0 31.0 2 16.238701695655337 16.0 2.0 30.0 2.0 31.0 3 19.661351765911114 22.0 15.0 31.0 2.0 31.0 4 21.76299172045479 28.0 2.0 35.0 2.0 37.0 5 22.542234637496993 30.0 2.0 35.0 2.0 37.0 6 22.789148701527715 30.0 2.0 35.0 2.0 37.0 7 22.569747750975463 30.0 2.0 35.0 2.0 37.0 8 22.98030039242469 30.0 2.0 35.0 2.0 37.0 9 25.732497634815516 31.0 17.0 37.0 2.0 39.0 10 26.433875599693227 31.0 20.0 37.0 2.0 39.0 11 23.57613234956587 30.0 7.0 37.0 2.0 39.0 12 24.70735390437377 31.0 10.0 37.0 2.0 39.0 13 21.424802528088314 27.0 2.0 35.0 2.0 39.0 14 24.712984163060575 30.0 11.0 38.0 2.0 40.0 15 25.762511685970676 31.0 12.0 38.0 2.0 40.0 16 26.112333667351496 31.0 10.0 38.0 2.0 40.0 17 25.41374913929677 31.0 8.0 38.0 2.0 40.0 18 25.482591402484424 31.0 10.0 38.0 2.0 40.0 19 25.872158279825115 31.0 10.0 38.0 2.0 40.0 20 26.126110517093707 31.0 8.0 39.0 2.0 40.0 21 23.99079537375513 30.0 2.0 37.0 2.0 40.0 22 24.896522479049224 31.0 2.0 38.0 2.0 40.0 23 24.873405249869844 31.0 2.0 38.0 2.0 40.0 24 23.971442846506523 31.0 2.0 38.0 2.0 40.0 25 23.76281957980888 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.5822901153801325 1101 2 10.25454409807098 1101 3 6.90463729520285 1101 4 8.31757049163162 1101 5 8.182291181689259 1101 6 7.38105744358576 1101 7 7.078717615850945 1101 8 5.901627630148326 1101 9 5.828618376519351 1101 10 5.344579323435049 1101 11 7.9928563902698855 1101 12 5.666185542422205 1101 13 10.026200603480298 1101 14 7.478982478115196 1101 15 6.619029971297596 1101 16 6.273587465235021 1101 17 2.0697013650925093 1101 18 4.755348354500388 1101 19 5.16550222367561 1101 20 3.624446134373791 1101 21 0.9087991838107001 1101 22 4.382171339477832 1101 23 5.576707650555509 1101 24 6.900514268907479 1101 25 7.295082042967994 1102 1 6.003667065536536 1102 2 10.680542914820837 1102 3 6.901320926146475 1102 4 8.436833607894119 1102 5 8.407976208345076 1102 6 6.6973911985745325 1102 7 6.4404992898557225 1102 8 6.905783387706258 1102 9 6.416047160876918 1102 10 5.849595715698815 1102 11 8.27489092679211 1102 12 5.804275410597679 1102 13 9.844311521741247 1102 14 7.40710689588748 1102 15 5.339415540875649 1102 16 5.88104324861526 1102 17 6.654501503683132 1102 18 6.521664949093392 1102 19 5.397199599279084 1102 20 5.744133520992605 1102 21 4.482885351877982 1102 22 5.738098213311332 1102 23 6.1327057808895304 1102 24 7.510493447627283 1102 25 7.058973301625823 1103 1 4.572169498479795 1103 2 8.73443093240773 1103 3 5.627025675412568 1103 4 7.411089985154479 1103 5 6.954008433114179 1103 6 6.330119312138695 1103 7 6.206169991320852 1103 8 5.589655587810814 1103 9 5.020736106065318 1103 10 4.39961046080947 1103 11 5.1785765342236445 1103 12 4.055938927172523 1103 13 8.009879677664092 1103 14 6.3135954148081375 1103 15 5.422744852815168 1103 16 5.162686926313949 1103 17 2.7789569399373377 1103 18 1.6853216024894593 1103 19 3.661409864557026 1103 20 3.899246723228501 1103 21 6.018289032793785 1103 22 1.4731171808854917 1103 23 3.9489254026104206 1103 24 5.517831150875228 1103 25 1.495980848441551 1104 1 5.017865577774263 1104 2 9.768808958642435 1104 3 6.444436590830765 1104 4 6.914981690617367 1104 5 7.059968723736283 1104 6 6.359221656483424 1104 7 6.356206500334178 1104 8 6.467064276438126 1104 9 5.327077799358534 1104 10 4.220818572542587 1104 11 7.326695672714504 1104 12 4.524578720443472 1104 13 8.402013668332284 1104 14 6.11067909553811 1104 15 5.508433578521139 1104 16 4.919744040910825 1104 17 5.428975323908588 1104 18 2.9276650668582143 1104 19 3.689015598284623 1104 20 3.041222767101459 1104 21 2.0943844326581065 1104 22 4.108585040007309 1104 23 4.5767354314551305 1104 24 5.58762809861814 1104 25 4.468826946119993 1105 1 3.229927051012208 1105 2 8.980722048202583 1105 3 5.966535159988158 1105 4 5.018988049917844 1105 5 2.1110021100637866 1105 6 4.328124677151148 1105 7 5.350642112416846 1105 8 2.9235030681710334 1105 9 3.6756840526706718 1105 10 3.2442313971318804 1105 11 6.379117506312806 1105 12 4.038940140687245 1105 13 4.75846305889932 1105 14 3.396010309104618 1105 15 4.067723456634546 1105 16 4.420379970958518 1105 17 5.057570978386593 1105 18 2.3864144044310827 1105 19 2.273520102789128 1105 20 1.5735598100267367 1105 21 2.4276541729866707 1105 22 3.9349125439818735 1105 23 1.3205828082436817 1105 24 4.250924336032025 1105 25 5.134189956188884 1106 1 6.542483624203282 1106 2 11.048514828235945 1106 3 7.800205257240542 1106 4 9.099322295975817 1106 5 8.810331729552033 1106 6 7.958021759579626 1106 7 7.880525976074669 1106 8 7.560657255688739 1106 9 6.839698152692705 1106 10 5.976213897997397 1106 11 8.29831997381666 1106 12 5.793923931956563 1106 13 4.899432435413971 1106 14 6.132085593150823 1106 15 6.327141201260485 1106 16 6.261745281127528 1106 17 6.800492956166469 1106 18 6.97691136726116 1106 19 6.458068618824758 1106 20 6.008848262486371 1106 21 6.225862714830374 1106 22 6.657267373554152 1106 23 7.212706309329512 1106 24 8.072311677485462 1106 25 7.15738071563873 1107 1 3.9285893704434436 1107 2 -13.690650500817025 1107 3 -5.4800436562958605 1107 4 1.669586247626487 1107 5 4.585113417404276 1107 6 4.6995923219457865 1107 7 4.609710173821547 1107 8 5.137368937844538 1107 9 3.5157021661516303 1107 10 2.883179003888209 1107 11 5.6858566269033055 1107 12 2.6120325647238367 1107 13 4.518953934233288 1107 14 3.860834834757835 1107 15 3.4652274189585057 1107 16 3.3647353574549186 1107 17 4.031467640977727 1107 18 -0.882047308198544 1107 19 1.8143143789291472 1107 20 2.2108971283258825 1107 21 -0.9623707330496458 1107 22 2.2381086676969453 1107 23 -0.44515373117577894 1107 24 2.9208622973597436 1107 25 3.4337706291047 1108 1 5.6355699620227675 1108 2 10.669125140208617 1108 3 0.7994631388720208 1108 4 6.448961654744387 1108 5 7.623238158329247 1108 6 7.62451607986608 1108 7 7.738085551330876 1108 8 7.250595828352029 1108 9 5.960574701237789 1108 10 5.311244971792515 1108 11 7.416468562119743 1108 12 3.006285540385587 1108 13 3.8887816863503026 1108 14 5.504958272905526 1108 15 5.387112596906039 1108 16 5.382974239435139 1108 17 0.4339906616445113 1108 18 4.135571644250085 1108 19 5.158593632200894 1108 20 5.680820368096118 1108 21 5.3159535696684195 1108 22 2.3678386374962024 1108 23 5.063393894216535 1108 24 5.8414446980229755 1108 25 6.490820800834435 1109 1 4.117170815988079 1109 2 -13.690650500817025 1109 3 -5.129313781998274 1109 4 2.708877692521874 1109 5 2.0992272796496927 1109 6 3.1697547267613544 1109 7 4.69821728706502 1109 8 4.744977199992437 1109 9 4.212447577357654 1109 10 3.6905092817797183 1109 11 1.7502335671949893 1109 12 2.093281877381923 1109 13 3.4229003036767516 1109 14 4.243536211031657 1109 15 4.273388469627694 1109 16 3.972298569723325 1109 17 4.589291811298072 1109 18 4.616896669626318 1109 19 2.797380924226136 1109 20 2.2067055020384814 1109 21 1.1577024332546344 1109 22 3.4590717016208323 1109 23 3.810261860587513 1109 24 4.736789814753635 1109 25 5.151704721131004 1110 1 3.4851269825131936 1110 2 9.68838880049302 1110 3 6.665070993535551 1110 4 7.731487407739575 1110 5 8.49189088478765 1110 6 3.98584879736692 1110 7 6.656216219789055 1110 8 7.421627907626469 1110 9 5.9002888989760045 1110 10 5.663551544404765 1110 11 3.2806809963376367 1110 12 5.924598159353227 1110 13 10.331967639770973 1110 14 7.93651548934265 1110 15 7.190340119020021 1110 16 6.916912208307608 1110 17 7.415724929923542 1110 18 7.48136088957536 1110 19 6.3227948694643565 1110 20 6.440839542880578 1110 21 4.723778841194566 1110 22 6.64631249260761 1110 23 7.0745103705349734 1110 24 7.673761907741934 1110 25 8.065478593914715 1111 1 4.923794772640491 1111 2 9.271719395099709 1111 3 6.961222674705816 1111 4 7.790294977813101 1111 5 8.00869909117809 1111 6 7.481382342106642 1111 7 7.174642489188852 1111 8 7.380565188455499 1111 9 5.844326574531298 1111 10 5.234162793229153 1111 11 6.983807689832172 1111 12 6.151042371491496 1111 13 7.703324987658014 1111 14 5.9319396015581916 1111 15 5.673231235406583 1111 16 5.242882679007309 1111 17 6.575439076786882 1111 18 6.425279692740162 1111 19 5.341481609249197 1111 20 4.79101942477736 1111 21 7.557848367558016 1111 22 7.102975464906848 1111 23 6.902848149928797 1111 24 6.910035468074916 1111 25 7.72159195622028 1112 1 -2.135740067304539 1112 2 7.588936472971696 1112 3 5.672707191946294 1112 4 -0.3583627887496341 1112 5 3.95830389018807 1112 6 2.8997230512108594 1112 7 5.632331595199634 1112 8 6.015209838249994 1112 9 4.561739901539209 1112 10 3.403356130424722 1112 11 -0.44479253745052816 1112 12 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2208 20 4.916093608934428 2208 21 7.479846849143893 2208 22 6.615379909010073 2208 23 2.4983802833981272 2208 24 4.1825493696070986 2208 25 5.3115129036327 2209 1 2.2834752648182786 2209 2 4.077278588626971 2209 3 4.348807520987354 2209 4 -0.35948092671390697 2209 5 4.28150976518063 2209 6 5.074490511155982 2209 7 5.481181999604598 2209 8 3.7700784106367884 2209 9 3.5716604158186698 2209 10 2.5843549534037074 2209 11 -1.1614895310226743 2209 12 2.6020881396128424 2209 13 1.9777808133983825 2209 14 3.216402465967363 2209 15 3.613917386261189 2209 16 3.191854588202677 2209 17 0.6606760012176451 2209 18 2.897926625757968 2209 19 3.522824427258641 2209 20 3.7013922032580027 2209 21 5.99533614988702 2209 22 4.645597475488721 2209 23 0.43942245369090216 2209 24 -2.894807600578236 2209 25 0.3581598729227018 2210 1 -2.8976531371324157 2210 2 -13.690650500817025 2210 3 -7.758252327261076 2210 4 -2.6931001610891023 2210 5 -1.2366347481477042 2210 6 -1.9951045451736178 2210 7 -1.868525323343583 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20 -6.223206090484563 2211 21 -4.000985702134784 2211 22 -5.551869125631548 2211 23 -7.403001078601061 2211 24 -21.48868350710523 2211 25 -21.27600612224249 2212 1 -1.1533196586305543 2212 2 -13.690650500817025 2212 3 -7.659451947183477 2212 4 -19.225717832435098 2212 5 -19.936056713465625 2212 6 -20.14952238167238 2212 7 -9.609319611998947 2212 8 -9.347467922965146 2212 9 -5.221349253802604 2212 10 -5.569704273026591 2212 11 -5.541173253826866 2212 12 -2.894487202852911 2212 13 -6.149032240590337 2212 14 -3.7373142947095097 2212 15 -3.6317010665994083 2212 16 -3.846521721225205 2212 17 -3.970568962521277 2212 18 -2.986541545072008 2212 19 -3.6968572810411153 2212 20 -3.898023590456745 2212 21 -1.8279918114344937 2212 22 -3.0532844745515675 2212 23 -3.268172228017395 2212 24 -2.245532822173722 2212 25 -2.728060916763038 2213 1 -18.371641576438773 2213 2 -13.690650500817025 2213 3 -8.557346650800673 2213 4 -5.773183270223116 2213 5 -19.936056713465625 2213 6 -10.54214910978298 2213 7 -20.00229906405374 2213 8 -10.576198748039321 2213 9 -8.438865509089517 2213 10 -7.395732459378465 2213 11 -6.502306547179558 2213 12 -5.358331025843881 2213 13 -18.746634980316365 2213 14 -12.300431364338952 2213 15 -7.098634183195582 2213 16 -6.189541416951485 2213 17 -4.971593203415157 2213 18 -5.0273707225229245 2213 19 -5.83367787317545 2213 20 -5.77825368956665 2213 21 -3.8975360321276327 2213 22 -4.79372936439438 2213 23 -4.6020195230114 2213 24 -3.641678898810298 2213 25 -4.548817182150323 2214 1 -18.371641576438773 2214 2 -13.690650500817025 2214 3 -11.99168662559455 2214 4 -19.225717832435098 2214 5 -19.936056713465625 2214 6 -20.14952238167238 2214 7 -15.875994262383594 2214 8 -14.511619122667723 2214 9 -13.605986912166204 2214 10 -13.399677891009262 2214 11 -14.111616457418824 2214 12 -11.971138045075577 2214 13 -18.746634980316365 2214 14 -16.284088753484927 2214 15 -23.250707915914475 2214 16 -17.74587325266914 2214 17 -22.896938825534974 2214 18 -17.225756376198497 2214 19 -14.460252053254917 2214 20 -13.074086020748162 2214 21 -10.677485619882459 2214 22 -11.748977914080267 2214 23 -11.636737589644074 2214 24 -10.517911064516502 2214 25 -10.305233679653762 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 138134.0 3 2.0 4 579.0 5 2525.0 6 3367.0 7 3227.0 8 2670.0 9 2586.0 10 2499.0 11 2336.0 12 2262.0 13 2355.0 14 3522.0 15 7436.0 16 10477.0 17 11330.0 18 11649.0 19 13700.0 20 16182.0 21 18228.0 22 19578.0 23 20925.0 24 22133.0 25 22356.0 26 18652.0 27 14898.0 28 16077.0 29 18755.0 30 18011.0 31 21003.0 32 26571.0 33 33275.0 34 40195.0 35 45538.0 36 44273.0 37 45188.0 38 32038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.625995003195634 19.625530184184534 13.648732399821819 25.099742412798015 2 13.739508449283422 21.525068734904146 40.72644716917687 24.008975646635562 3 17.57815804196427 26.150277728723665 29.74732102709914 26.524243202212926 4 11.402694217076363 18.863253385628894 39.01419837463839 30.719854022656357 5 12.404733042117343 37.902694584127055 36.578141968604996 13.114430405150607 6 29.12911236073155 38.43020811435779 19.03050767290309 13.410171852007569 7 25.949961956764984 32.36483848215981 23.09509882752756 18.59010073354765 8 24.070067335115393 36.40265822842388 21.94296908285661 17.58430535360412 9 25.57247362691099 16.740904104099616 21.06176115768368 36.62486111130571 10 14.14112281648919 30.1708448473052 34.54830895567979 21.13972338052582 11 31.543326322913686 23.779571033559478 25.121890765095532 19.555211878431308 12 22.375243273759118 26.03630907281128 31.691699722185078 19.896747931244523 13 26.557731370763584 22.430297754251846 25.894980504384428 25.116990370600146 14 20.436981054764196 22.80342823972638 28.73996255342397 28.019628152085456 15 22.134503598449896 30.64420326628529 24.687442332533678 22.53385080273113 16 21.470205290614256 29.450441269447182 27.257662672097972 21.821690767840597 17 20.88570193139233 28.757999423464973 28.756125684635343 21.60017296050735 18 20.73517306678002 28.607518634516115 29.801719934205195 20.85558836449867 19 21.728430846000176 28.12721119172045 28.284925107202834 21.85943285507654 20 22.228960142394712 28.2320258960732 28.62998430626093 20.90902965527116 21 21.89855719608518 26.98636253833935 27.56524094312966 23.549839322445813 22 21.75277406574077 28.117506251165196 28.7402745816309 21.389445101463135 23 21.71746114926166 27.994669977147034 28.51646296096501 21.771405912626296 24 21.453754268315233 28.24946684582291 28.483993047422985 21.812785838438874 25 22.023030510241544 27.863610504692698 28.342408591181822 21.77095039388394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 935.0 1 935.0 2 1849.0 3 2763.0 4 2763.0 5 2763.0 6 3866.0 7 4969.0 8 4969.0 9 4969.0 10 6059.0 11 7149.0 12 7149.0 13 7149.0 14 9157.5 15 11166.0 16 11166.0 17 11166.0 18 15356.0 19 19546.0 20 19546.0 21 19546.0 22 26230.0 23 32914.0 24 32914.0 25 32914.0 26 41256.0 27 49598.0 28 49598.0 29 49598.0 30 58092.0 31 66586.0 32 66586.0 33 66586.0 34 72717.5 35 78849.0 36 78849.0 37 78849.0 38 82265.0 39 85681.0 40 85681.0 41 85681.0 42 85994.5 43 86308.0 44 86308.0 45 86308.0 46 83989.5 47 81671.0 48 81671.0 49 81671.0 50 74990.5 51 68310.0 52 68310.0 53 68310.0 54 59115.0 55 49920.0 56 49920.0 57 49920.0 58 41245.5 59 32571.0 60 32571.0 61 32571.0 62 25881.0 63 19191.0 64 19191.0 65 19191.0 66 14362.5 67 9534.0 68 9534.0 69 9534.0 70 6937.0 71 4340.0 72 4340.0 73 4340.0 74 3016.5 75 1693.0 76 1693.0 77 1693.0 78 1146.5 79 600.0 80 600.0 81 600.0 82 383.0 83 166.0 84 166.0 85 166.0 86 110.0 87 54.0 88 54.0 89 54.0 90 33.5 91 13.0 92 13.0 93 13.0 94 8.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.286458828995762 2 36.32069662380411 3 5.692257309679623 4 13.791684627140564 5 13.528155491986363 6 14.783942496627164 7 9.318686916751103 8 7.696646196391484 9 0.11629989979455083 10 1.4942647775047164 11 5.706252484143468 12 5.797081166413821 13 16.081854976404138 14 1.6323971494628653 15 2.924991462943577 16 3.045070059843366 17 2.9014795698443177 18 1.558082773059849 19 1.5011224129920004 20 0.30033644399411086 21 4.406660583430833 22 1.6602475466459166 23 0.11741951375165843 24 9.175796185475248 25 0.12175801783545033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.67534048987609 #Duplication Level Percentage of deduplicated Percentage of total 1 97.58039864370205 93.36037865374038 2 1.8998258547541997 3.6353297105015594 3 0.2678391142527376 0.768767953579125 4 0.10365362192506782 0.39668382282759 5 0.0441426529313213 0.21116816746652967 6 0.0266274204760187 0.15285525122461127 7 0.015640994859776224 0.10475202561666443 8 0.010620062628080787 0.081286248637235 9 0.008264612892357488 0.071164768724399 >10 0.0376463941886412 0.6372635061235471 >50 0.003708891817491146 0.23930552339452987 >100 0.0014833959757659112 0.2539027779216803 >500 1.483395965442565E-4 0.08714159024206589 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3995174463844867E-4 0.0 5 0.0 0.0 0.0 1.3995174463844867E-4 0.0 6 0.0 0.0 0.0 1.3995174463844867E-4 0.0 7 0.0 0.0 0.0 1.3995174463844867E-4 0.0 8 0.0 0.0 0.0 1.3995174463844867E-4 0.0 9 0.0 0.0 0.0 1.3995174463844867E-4 0.0 10 0.0 0.0 0.0 1.3995174463844867E-4 0.0 11 0.0 0.0 0.0 1.3995174463844867E-4 0.0 12 0.0 0.0 0.0 2.7990348927689734E-4 0.0 13 0.0 0.0 0.0 2.7990348927689734E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTAGG 40 4.5931397E-4 20.51829 2 GGCCAGA 45 0.0011909215 17.808678 1 GTCCTAC 165 5.14774E-10 12.951765 1 TGACAGT 65 3.6750565E-4 12.825983 6 TCTACAC 80 9.923133E-5 12.335289 3 TAGGACA 70 5.316482E-4 12.276188 4 GTTTAGA 100 1.2616378E-4 12.020858 1 GCACTGT 80 1.5945491E-4 11.72375 6 TCTACTC 75 7.9870655E-4 11.695681 3 TTCTAGA 95 9.0670784E-4 11.51904 2 GTGTAGA 105 1.9868038E-4 11.448436 1 TCCAACG 60 0.0010128415 11.369778 18 GTATAGC 95 0.0011071817 11.247585 1 TAGGCAG 65 0.0053223646 11.22922 5 GTCCTAA 145 2.778328E-6 11.053661 1 GTCCTGT 85 0.006203844 10.999476 1 TCTATAC 100 6.445988E-5 10.964703 3 TATAAAC 70 0.006355022 10.964702 3 GTATCAA 550 0.0 10.928052 1 TTAAGGG 70 0.007403973 10.741665 4 >>END_MODULE