FastQCFastQC Report
Thu 26 May 2016
SRR1926621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23600.33028611734673885No Hit
GGTATCAACGCAGAGTACTTTTTTT13470.18851500002799035No Hit
TATCAACGCAGAGTACTTTTTTTTT11200.1567459539950625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7460.10440400150028271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT853.783478E-612.33731651
TCCAATA550.002967279212.1436552
TAGATCA550.00297355312.1402324
CCTACAC1052.569759E-711.8107193
TAGGACC1655.456968E-1211.5621264
CCCTACA855.0827133E-511.2252282
GGTATAT855.1266972E-511.2157421
GCATTAG600.00571904111.1222771
GGTATCA6200.011.0710231
CTACACT1303.063178E-811.0062554
ATAGGAC1053.2895878E-610.9022023
AAATAGC700.001440771610.9006656
CTCTTAT909.165631E-510.5926451
TGGCGAG1752.4738256E-1010.28533818
TGTTGGA750.00255073310.1775412
TAGAACA2350.010.1476124
CCATAAG856.374619E-410.0941681
GTAGGAC2002.3646862E-1110.0163973
GTCCTAC2053.8198777E-119.76590251
GTAGAAC1502.4827386E-79.5394273
Produced by FastQC (version 0.11.5)