FastQCFastQC Report
Thu 26 May 2016
SRR1926620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA2791.4242687222420747No Hit
CCCATGTACTCTGCGTTGATACCAC2481.2660166419929553No Hit
GTACATGGGAAGCAGTGGTATCAAC2111.0771351268569094No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1430.7300015314717443No Hit
GAGTACATGGGAAGCAGTGGTATCA1160.5921690744805758No Hit
GCGTTGATACCACTGCTTCCCATGT810.4134973709735056No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA800.40839246515901784No Hit
ACTCTGCGTTGATACCACTGCTTCC790.4032875593445301No Hit
CATGTACTCTGCGTTGATACCACTG780.3981826535300424No Hit
ACGCAGAGTACATGGGAAGCAGTGG780.3981826535300424No Hit
GGTATCAACGCAGAGTACATGGGAA510.2603501965388738No Hit
GNACATGGGAAGCAGTGGTATCAAC480.2450354790954107No Hit
GTATCAACGCAGAGTACATGGGAAG470.23993057328092296No Hit
TATCAACGCAGAGTACATGGGAAGC430.21951095002297208No Hit
GCTTCCCATGTACTCTGCGTTGATA400.20419623257950892No Hit
CNCATGTACTCTGCGTTGATACCAC390.1990913267650212No Hit
AAACAAAAAAAAAAAAAAAAAAAAA370.18888151513604573No Hit
ATACCACTGCTTCCCATGTACTCTG340.17356679769258257No Hit
CCACTGCTTCCCATGTACTCTGCGT330.16846189187809485No Hit
GTACTCTGCGTTGATACCACTGCTT300.15314717443463166No Hit
GATACCACTGCTTCCCATGTACTCT300.15314717443463166No Hit
CATGGGAAGCAGTGGTATCAACGCA290.14804226862014394No Hit
CAGTGGTATCAACGCAGAGTACATG280.14293736280565622No Hit
GTATCAACGCAGAGTACTTTTTTTT260.13272755117668078No Hit
GTGGTATCAACGCAGAGTACATGGG260.13272755117668078No Hit
CTGCTTCCCATGTACTCTGCGTTGA260.13272755117668078No Hit
NNANNNNAAAANNAAAAAAAAAANA240.12251773954770535No Hit
NNAANANAAAAANAAAAAAAAAAAA240.12251773954770535No Hit
GGGAAGCAGTGGTATCAACGCAGAG240.12251773954770535No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA230.11741283373321763No Hit
GCAGAGTACATGGGAAGCAGTGGTA230.11741283373321763No Hit
GAACAAAAAAAAAAAAAAAAAAAAA230.11741283373321763No Hit
GNGTACATGGGAAGCAGTGGTATCA210.10720302210424218No Hit
NNAAAANAAAAAAAAAAAAAAAAAA210.10720302210424218No Hit
ANAAAAANAAAAAAAAAAAAAAAAA200.10209811628975446No Hit
GGTATCAACGCAGAGTACTTTTTTT200.10209811628975446No Hit
ATGGGAAGCAGTGGTATCAACGCAG200.10209811628975446No Hit
TACCACTGCTTCCCATGTACTCTGC200.10209811628975446No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)