FastQCFastQC Report
Thu 26 May 2016
SRR1926620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC7053.5989585992138444No Hit
GTACATGGGAAGCAGTGGTATCAAC6163.144621981724437No Hit
GTATCAACGCAGAGTACTTTTTTTT4382.2359487467456223No Hit
GGTATCAACGCAGAGTACTTTTTTT3191.6284649548215835No Hit
GAGTACATGGGAAGCAGTGGTATCA2671.363009852468222No Hit
CATGTACTCTGCGTTGATACCACTG2501.2762264536219308No Hit
TATCAACGCAGAGTACTTTTTTTTT2361.2047577722191025No Hit
ACGCAGAGTACATGGGAAGCAGTGG2121.0822400326713972No Hit
GCGTTGATACCACTGCTTCCCATGT2101.0720302210424217No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1910.9750370105671551No Hit
GTATCAACGCAGAGTACATGGGAAG1590.8116800245035478No Hit
ACTCTGCGTTGATACCACTGCTTCC1550.791260401245597No Hit
GGTATCAACGCAGAGTACATGGGAA1440.735106437286232No Hit
TATCAACGCAGAGTACATGGGAAGC1280.6534279442544285No Hit
GTGGTATCAACGCAGAGTACATGGG1000.5104905814487722No Hit
GCTTCCCATGTACTCTGCGTTGATA990.5053856756342846No Hit
CATGGGAAGCAGTGGTATCAACGCA900.45944152330389504No Hit
GAGTACTTTTTTTTTTTTTTTTTTT890.45433661748940735No Hit
CTGCTTCCCATGTACTCTGCGTTGA830.42370718260248097No Hit
CAGTGGTATCAACGCAGAGTACATG790.4032875593445301No Hit
GCAGAGTACATGGGAAGCAGTGGTA790.4032875593445301No Hit
GCAGTGGTATCAACGCAGAGTACAT750.3828679360865792No Hit
GGGAAGCAGTGGTATCAACGCAGAG740.37776303027209146No Hit
ACATGGGAAGCAGTGGTATCAACGC730.37265812445760377No Hit
GATACCACTGCTTCCCATGTACTCT730.37265812445760377No Hit
ATACCACTGCTTCCCATGTACTCTG640.32671397212721426No Hit
CCACTGCTTCCCATGTACTCTGCGT630.3216090663127265No Hit
GTACTCTGCGTTGATACCACTGCTT630.3216090663127265No Hit
GCAGAGTACTTTTTTTTTTTTTTTT610.31139925468375107No Hit
CCATGTACTCTGCGTTGATACCACT590.30118944305477563No Hit
GTACTTTTTTTTTTTTTTTTTTTTT570.2909796314258002No Hit
GTTGATACCACTGCTTCCCATGTAC550.28076981979682475No Hit
CTTCCCATGTACTCTGCGTTGATAC460.23482566746643527No Hit
AAGCAGTGGTATCAACGCAGAGTAC430.21951095002297208No Hit
GTGGTATCAACGCAGAGTACTTTTT420.21440604420848436No Hit
TACCACTGCTTCCCATGTACTCTGC410.20930113839399664No Hit
ATCAACGCAGAGTACTTTTTTTTTT380.19398642095053348No Hit
ATCAACGCAGAGTACATGGGAAGCA370.18888151513604573No Hit
ATGGGAAGCAGTGGTATCAACGCAG340.17356679769258257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA300.15314717443463166No Hit
GTACATGGTAAGCAGTGGTATCAAC250.12762264536219306No Hit
TCAACGCAGAGTACATGGGAAGCAG220.1123079279187299No Hit
TTCCCATGTACTCTGCGTTGATACC210.10720302210424218No Hit
CTCTGCGTTGATACCACTGCTTCCC200.10209811628975446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1151.0007546E-49.06004611
AAGCAGT1151.0007546E-49.06004610
GCAGTGG1201.5911151E-48.68254612
CTCTGCG1553.4116529E-68.5771719
CTGCGTT1553.5339217E-68.55523511
TGCGTTG1451.4566542E-58.49201912
GAAGCAG1050.00413491768.139569
ACTCTGC1553.2626343E-57.96451668
TCTGCGT1553.37067E-57.94414710
GGAAGCA1100.0062860037.76958048
GTGGTAT1250.00233168627.57749315
TACTCTG1656.896143E-57.50105147
AGTACTT1657.357442E-57.46268312
GAGTACT1657.357442E-57.46268311
CCATGTA1552.737829E-47.3897572
GTACTCT1709.673842E-57.29919676
GTATCAA2757.592462E-97.28898761
TGGTATC1300.00342558677.28605216
AGTGGTA1300.00342558677.28605214
TGTACTC1603.947549E-47.15882735
Produced by FastQC (version 0.11.5)