##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19589 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.830925519424166 31.0 28.0 31.0 16.0 34.0 2 28.035836438817704 31.0 28.0 31.0 16.0 34.0 3 28.428761039358825 31.0 28.0 33.0 20.0 34.0 4 32.122926132012864 35.0 32.0 37.0 25.0 37.0 5 31.823829700342028 35.0 32.0 37.0 19.0 37.0 6 31.943641839808055 35.0 32.0 37.0 21.0 37.0 7 31.74700086783399 35.0 32.0 37.0 17.0 37.0 8 31.707386798713564 35.0 32.0 37.0 17.0 37.0 9 32.83051712695901 37.0 32.0 39.0 16.0 39.0 10 32.668640563581604 37.0 32.0 39.0 16.0 39.0 11 32.923885854305986 37.0 32.0 39.0 17.0 39.0 12 32.681147582827094 37.0 32.0 39.0 13.0 39.0 13 32.77063658175506 37.0 32.0 39.0 15.0 39.0 14 33.22890397672163 38.0 32.0 40.0 10.0 41.0 15 33.2720914799122 38.0 32.0 40.0 10.0 41.0 16 33.10184287099903 38.0 32.0 40.0 10.0 41.0 17 33.22869978048905 38.0 32.0 40.0 10.0 41.0 18 33.190923477461844 38.0 32.0 40.0 10.0 41.0 19 33.116034509163306 38.0 32.0 40.0 8.0 41.0 20 33.273520853540255 38.0 33.0 40.0 2.0 41.0 21 33.120118433814895 38.0 32.0 40.0 2.0 41.0 22 33.102710704987494 38.0 32.0 40.0 2.0 41.0 23 32.69258257185155 38.0 32.0 40.0 2.0 41.0 24 32.78447087651233 38.0 32.0 40.0 2.0 41.0 25 32.573995609781 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.79804277887202 1101 2 0.550929379836564 1101 3 1.1408288650384968 1101 4 1.717306726289337 1101 5 1.7711281887819084 1101 6 2.732865545467412 1101 7 1.8432739256662565 1101 8 2.4255968226804967 1101 9 2.0719642845560386 1101 10 0.6937532602450815 1101 11 1.8763244390917038 1101 12 1.2438848853536015 1101 13 0.8790657175589303 1101 14 1.370968598264291 1101 15 0.32456536731064745 1101 16 1.7824375996879738 1101 17 0.5286447227484743 1101 18 1.8626490763536054 1101 19 1.0865807907400935 1101 20 1.7205386227285757 1101 21 1.408103140002403 1101 22 1.5812604858566104 1101 23 1.1723961965760523 1101 24 1.1372049167617106 1101 25 1.528756270675693 1102 1 0.8824830065760025 1102 2 0.9323335544096203 1102 3 0.23665429198346644 1102 4 -0.10053008585487788 1102 5 -1.7383594772522457 1102 6 -1.432219843526891 1102 7 -1.2724755999504431 1102 8 -2.6000198756117214 1102 9 -1.9849617116488965 1102 10 -1.8669678023735123 1102 11 -1.2147571548361888 1102 12 -2.232395949561006 1102 13 -1.0336477549268395 1102 14 -2.3652742859861853 1102 15 -1.8471613879075655 1102 16 -1.8968792883575674 1102 17 -1.3897613493577872 1102 18 -1.2597418154870041 1102 19 -2.6971004236811567 1102 20 -2.5318332937799646 1102 21 -1.221877884665524 1102 22 -1.4604852446936718 1102 23 -2.1454406155681625 1102 24 -2.5041613071472106 1102 25 -1.9503708640491624 1103 1 0.145508216660037 1103 2 0.4163671678550003 1103 3 0.3316122751767452 1103 4 -0.6467485732498339 1103 5 -0.003065359605187723 1103 6 -0.19524505361092537 1103 7 0.22668406391510487 1103 8 -0.036154329393234264 1103 9 1.369660137090598 1103 10 0.9960574077105235 1103 11 1.1869235174327173 1103 12 1.6365116134642008 1103 13 0.6873606484345061 1103 14 1.7506921005684362 1103 15 1.0259478557899158 1103 16 1.2930366780289901 1103 17 1.4724235245917896 1103 18 1.218409445017194 1103 19 0.9953365511087569 1103 20 1.2127045213460832 1103 21 1.8302229556706067 1103 22 2.1748088729533848 1103 23 1.694055182751164 1103 24 1.944578188651107 1103 25 1.9176963628415962 1104 1 -0.4600217372570121 1104 2 0.0025422830623718085 1104 3 -0.40755823173569894 1104 4 -0.4439835962913108 1104 5 0.2227410920077162 1104 6 -0.5251989706616165 1104 7 0.2626287643759291 1104 8 0.29656456461598424 1104 9 -0.9280357154439649 1104 10 -1.9191499655613669 1104 11 -1.4462562060695845 1104 12 -0.11095382432381484 1104 13 -0.5080310566346213 1104 14 -0.6612894662518372 1104 15 0.7761782705364553 1104 16 0.6856634061395894 1104 17 1.947999561458154 1104 18 0.507810366676182 1104 19 -0.23599985442119475 1104 20 -0.956880732110136 1104 21 -0.9144775051588852 1104 22 0.1941637116630659 1104 23 0.07562200302766087 1104 24 0.07268878772944731 1104 25 0.6255304642240844 1105 1 1.7247289958808167 1105 2 -0.33861118712335525 1105 3 0.6207897643542353 1105 4 0.2060652795640152 1105 5 -0.42730778384761336 1105 6 0.013412955047080288 1105 7 1.5011429383739774 1105 8 -0.4101803034192102 1105 9 -0.7550151875847284 1105 10 -0.3580551464020303 1105 11 -0.7316912011820023 1105 12 -1.3660857891332014 1105 13 -0.16199000091614835 1105 14 -0.003420453544116242 1105 15 0.3177227475648081 1105 16 0.9319977169900255 1105 17 -0.6072301550618846 1105 18 0.38464321125096035 1105 19 0.6291027848749948 1105 20 1.5269902356318 1105 21 1.1471060725537257 1105 22 1.7142028123473239 1105 23 2.5702456589416443 1105 24 0.807781651854576 1105 25 1.9471335922788242 1106 1 1.24461535951718 1106 2 1.0922600249978558 1106 3 0.43518370374817295 1106 4 1.3755728553215931 1106 5 1.5906846403948123 1106 6 -0.5131021964680684 1106 7 0.9168626353436728 1106 8 0.9165242420353366 1106 9 1.739302994233455 1106 10 1.7764145505676652 1106 11 0.5315663745755757 1106 12 2.2106187563213453 1106 13 0.07664636272021852 1106 14 0.7247992434255792 1106 15 1.461662141504199 1106 16 1.5939295351718457 1106 17 1.9429592388775063 1106 18 0.9755523021600538 1106 19 0.9756936939659013 1106 20 2.9332402356318 1106 21 1.382901527099179 1106 22 0.21230887295338619 1106 23 -0.7318376743916915 1106 24 -0.2670289542060331 1106 25 0.49448207712730863 1107 1 1.755032026183848 1107 2 2.1765403280281603 1107 3 1.590486734051204 1107 4 1.539398612897351 1107 5 2.0878437313039058 1107 6 0.8618978035319316 1107 7 2.228415665646704 1107 8 2.5292136359747275 1107 9 2.6995302669607284 1107 10 1.7934600051131184 1107 11 2.9652784957877003 1107 12 3.330883907836494 1107 13 4.141040302114156 1107 14 3.2996098494861883 1107 15 3.8328742627163237 1107 16 1.6592704442627522 1107 17 3.332163784332053 1107 18 3.142218968826718 1107 19 3.3563755121477215 1107 20 3.5269902356318 1107 21 3.45013637558403 1107 22 2.8354149335594485 1107 23 3.752063840759824 1107 24 4.201721045793967 1107 25 4.522891168036402 1108 1 1.6994764706282908 1108 2 2.4846211361089665 1108 3 1.8379614815259515 1108 4 1.7540450775438146 1108 5 -0.05862091516074486 1108 6 2.5563422479763744 1108 7 2.809223746454787 1108 8 2.801940908702001 1108 9 3.5379141053445693 1108 10 3.783358995012108 1108 11 2.816288596797797 1108 12 3.762702089654681 1108 13 3.0072019182757757 1108 14 3.387993687870022 1108 15 2.8783288081708633 1108 16 3.3890684240607314 1108 17 3.2867092388775063 1108 18 2.0588856354933895 1108 19 3.482638138410344 1108 20 4.165879124520686 1108 21 3.2405404159880646 1108 22 3.7470310951756076 1108 23 4.848023436719423 1108 24 5.007276601349524 1108 25 4.348648743793973 1109 1 0.6026510738028961 1109 2 0.10208145356928355 1109 3 0.8744694180338861 1109 4 -0.1324628589641179 1109 5 -0.6887796453194746 1109 6 -1.10953076789664 1109 7 0.34096977820081875 1109 8 -0.836154329393235 1109 9 0.7410887085191717 1109 10 -1.2325140208609078 1109 11 -1.498790768281566 1109 12 0.6650830420356328 1109 13 -0.08406792299406618 1109 14 -0.7350221851458514 1109 15 -0.8597664299243704 1109 16 0.20732239231470118 1109 17 0.10099495316322304 1109 18 -0.3244476978399433 1109 19 -0.17609202031981397 1109 20 -0.7587240500824848 1109 21 -0.512634187186535 1109 22 -0.4537625556180416 1109 23 0.00834089703688079 1109 24 0.8588639029368252 1109 25 0.0034106485558780264 1110 1 2.209577480729301 1110 2 0.5704797219675513 1110 3 1.0147291582936262 1110 4 1.8424289159276555 1110 5 -0.15458051112034 1110 6 1.558867500501627 1110 7 0.834476271707306 1110 8 1.4686075753686723 1110 9 2.184378751809213 1110 10 1.2177024293555405 1110 11 -0.277145746636549 1110 12 1.8763384532910408 1110 13 1.9895251505990075 1110 14 2.269306819183157 1110 15 2.8328742627163237 1110 16 1.8410886260809392 1110 17 1.0291334813017485 1110 18 -0.19111436450661046 1110 19 -0.15877600300379413 1110 20 0.5875962962378622 1110 21 1.207712133159788 1110 22 0.9263240244685349 1110 23 1.4793365680325508 1110 24 2.3229331670060915 1110 25 0.2804669256121599 1111 1 -1.3116346404828185 1111 2 -0.21220426071642962 1111 3 1.1601837037481744 1111 4 1.4103942838930195 1111 5 1.9112203546805233 1111 6 1.9761835178176455 1111 7 1.140969778200816 1111 8 0.8781313848924768 1111 9 2.312517279947741 1111 10 1.9103431219962346 1111 11 1.529780660289859 1111 12 0.46508304203563 1111 13 1.8587892198630769 1111 14 2.3221206719970056 1111 15 2.2545192843613435 1111 16 2.578750963743275 1111 17 1.8724235245917953 1111 18 2.161266587874337 1111 19 0.4810508368230444 1111 20 1.6127045213460889 1111 21 1.1730800985277483 1111 22 1.1748088729533848 1111 23 1.0369123256083057 1111 24 2.030292474365396 1111 25 1.003410648555878 1112 1 -0.5783013071494878 1112 2 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0.43211535951718005 2203 2 2.248510024997856 2203 3 -0.15856629625182705 2203 4 0.9693228553215931 2203 5 1.6219346403948123 2203 6 0.7368978035319316 2203 7 1.0731126353436728 2203 8 1.1665242420353366 2203 9 0.520552994233455 2203 10 0.8389145505676652 2203 11 1.5940663745755757 2203 12 2.2418687563213453 2203 13 1.6078963627202185 2203 14 2.381049243425579 2203 15 2.680412141504199 2203 16 2.3751795351718457 2203 17 1.5367092388775063 2203 18 2.069302302160054 2203 19 2.0381936939659013 2203 20 2.0894902356318 2203 21 2.570401527099179 2203 22 2.962308872953386 2203 23 3.2056623256083085 2203 24 2.920471045793967 2203 25 2.7444820771273086 2204 1 1.2095774807293012 2204 2 1.2977524492402814 2204 3 -0.3489072053427371 2204 4 0.7818228553215931 2204 5 1.148449791909961 2204 6 1.1043220459561738 2204 7 1.5617489989800397 2204 8 1.8625469693080632 2204 9 1.4571060245364862 2204 10 1.1267933384464541 2204 11 1.9349754654846691 2204 12 0.8460354229880096 2204 13 1.504676665750523 2204 14 3.3299128797892124 2204 15 2.2268136566557146 2204 16 2.1138158988082125 2204 17 1.726103178271444 2204 18 1.6573704839782337 2204 19 1.68970884548105 2204 20 0.739111447753011 2204 21 0.6925606180082724 2204 22 0.3505664487109641 2204 23 2.176306265002246 2204 24 1.1411149851879046 2204 25 2.6138002589454885 2205 1 1.52586535951718 2205 2 1.4672600249978558 2205 3 1.153933703748173 2205 4 0.8443228553215931 2205 5 1.1219346403948123 2205 6 1.5806478035319316 2205 7 0.6668626353436728 2205 8 1.1040242420353366 2205 9 1.364302994233455 2205 10 0.7451645505676652 2205 11 1.2190663745755757 2205 12 1.7418687563213453 2205 13 1.6703963627202185 2205 14 1.8810492434255792 2205 15 1.992912141504199 2205 16 2.3751795351718457 2205 17 2.5679592388775063 2205 18 2.319302302160054 2205 19 2.0694436939659013 2205 20 2.1519902356318 2205 21 2.007901527099179 2205 22 2.212308872953386 2205 23 2.1431623256083085 2205 24 2.920471045793967 2205 25 2.4944820771273086 2206 1 1.1161431372949586 2206 2 1.5957322472200772 2206 3 1.893517037081505 2206 4 1.8929339664327074 2206 5 1.580267973728148 2206 6 1.8063422479763744 2206 7 1.8370015242325586 2206 8 2.0241631309242223 2206 9 2.482358549789012 2206 10 2.3111367727898866 2206 11 1.9273997079089114 2206 12 2.5404798674324596 2206 13 2.729424140497997 2206 14 3.721327021203358 2206 15 3.0449954748375347 2206 16 3.2501795351718457 2206 17 3.5089314610997278 2206 18 3.586663413271168 2206 19 2.9548603606325656 2206 20 2.721434680076243 2206 21 3.4349848604325146 2206 22 3.3303644285089433 2206 23 3.5702456589416443 2206 24 2.5350543791273026 2206 25 4.098648743793973 2207 1 -0.49986993460046847 2207 2 -0.5088429161786152 2207 3 -2.057463355075356 2207 4 -1.1887653799725229 2207 5 -0.09130065372283624 2207 6 -0.9028080788210104 2207 7 -0.3312991293622112 2207 8 -0.15884340502348593 2207 9 0.573861817762868 2207 10 -1.2787325082558638 2207 11 0.3146546098696916 2207 12 0.8264275798507583 2207 13 -0.5336477549268395 2207 14 -6.629980168339127 2207 15 -2.6118672702605075 2207 16 -2.455702817769332 2207 17 -0.036820172887196634 2207 18 0.09319936098358284 2207 19 0.9793701645541333 2207 20 0.20346082386709696 2207 21 0.13106329180506293 2207 22 0.2748088729533862 2207 23 -0.027793556744633463 2207 24 1.5252504575586698 2207 25 -0.009194393460923322 2208 1 0.3105875817394015 2208 2 0.3457322472200772 2208 3 -1.1620385184740485 2208 4 -0.8848438113450747 2208 5 0.16360130706147658 2208 6 -0.7492133075791791 2208 7 0.003668190899229984 2208 8 -1.0591702024091063 2208 9 -0.5454192279887664 2208 10 -0.07775211609899912 2208 11 0.3162885967977971 2208 12 -1.0428534659008761 2208 13 -0.9650203039464493 2208 14 -1.44533964546331 2208 15 -2.093893414051358 2208 16 -1.6387093537170436 2208 17 -2.2132907611224937 2208 18 -2.9133365867288354 2208 19 -2.795139639367431 2208 20 -3.1952319865904215 2208 21 -1.592792917345264 2208 22 -2.558524460379946 2208 23 -2.235309896613913 2208 24 -1.992723398650476 2208 25 -3.0124623673171342 2209 1 -1.676340522835762 2209 2 -2.2147252691197927 2209 3 -1.910404531545943 2209 4 -3.36523596820782 2209 5 -1.9736535948993037 2209 6 -2.3733963141151264 2209 7 -2.684240305832798 2209 8 -1.3941375226705475 2209 9 -1.9849617116488965 2209 10 -3.6022619200205703 2209 11 -1.7441689195420729 2209 12 -1.9971018319139482 2209 13 -3.504235990220959 2209 14 -2.6005684036332433 2209 15 -3.553043740848743 2209 16 -2.6027616412987413 2209 17 -2.2132907611224937 2209 18 -2.171506521369359 2209 19 -2.0794533648576277 2209 20 -3.355362705544671 2209 21 -2.01599553172435 2209 22 -3.754602891752498 2209 23 -3.204264144979927 2209 24 -3.2394554247942686 2209 25 -2.891547334637398 2210 1 0.20957748072930116 2210 2 0.6613888128766447 2210 3 1.0450321885966574 2210 4 1.3272774007761399 2210 5 1.9969346403948123 2210 6 2.2558371974713225 2210 7 2.5011429383739774 2210 8 1.7716378783989697 2210 9 2.9116514790819394 2210 10 2.1570963687494853 2210 11 1.6016421321513334 2210 12 1.2096717866243765 2210 13 2.2016463627202185 2210 14 0.5117310616073993 2210 15 2.1965106263526835 2210 16 1.628967413959721 2210 17 1.9988304509987174 2210 18 1.8694916960994448 2210 19 1.659405815178026 2210 20 1.4360811447227064 2210 21 1.1168030422506945 2210 22 0.6535967517412615 2210 23 0.9035789922749728 2210 24 1.5653574094303337 2210 25 -0.14377549863026928 2211 1 -1.28306321191139 2211 2 -0.6693471178592887 2211 3 -0.6683877248232548 2211 4 0.7246799981787362 2211 5 0.0255060689662443 2211 6 -1.10953076789664 2211 7 -0.4590302217991855 2211 8 0.1924170991781935 2211 9 1.369660137090598 2211 10 0.3674859791390972 2211 11 -0.41307648256728413 2211 12 0.3222258991784912 2211 13 -0.7983536372797815 2211 14 1.2078349577112917 2211 15 -0.25976642992436894 2211 16 -0.9641061791138696 2211 17 -0.6990050468367812 2211 18 0.7612665878743385 2211 19 0.4810508368230444 2211 20 0.6412759499175138 2211 21 1.630222955670611 2211 22 0.974808872953389 2211 23 1.3511980398940224 2211 24 1.2588639029368238 2211 25 0.6891249342701684 2212 1 0.7980427788720199 2212 2 -0.191006104034404 2212 3 0.689215961812689 2212 4 0.9431131779022408 2212 5 0.9646765758786842 2212 6 0.5715752228867714 2212 7 0.7787577966339931 2212 8 -0.21956446764208337 2212 9 -0.34739055415364106 2212 10 0.7260113247612168 2212 11 0.5214857294142874 2212 12 0.1793687563213453 2212 13 1.2016463627202185 2212 14 -0.016128175929260635 2212 15 -0.1593056004312814 2212 16 0.6856634061395894 2212 17 0.5931608517807305 2212 18 0.2174877860310218 2212 19 0.957548532675581 2212 20 0.559248300147928 2212 21 0.6984257206475633 2212 22 0.968357260050162 2212 23 1.849815551414757 2212 24 2.266237174826223 2212 25 1.6577885287402125 2213 1 1.1228481181378704 2213 2 1.114889335342685 2213 3 0.31880439340334377 2213 4 -1.6664530067473713 2213 5 -0.45134122167415214 2213 6 -0.827757368881862 2213 7 0.3705264284471177 2213 8 0.721481138587059 2213 9 0.6336995459575903 2213 10 0.028569722981458767 2213 11 1.154411202161782 2213 12 0.04143772183858374 2213 13 0.6154394661684961 2213 14 -3.616795584160627 2213 15 -3.21937234125442 2213 16 -1.6291308096557415 2213 17 -0.3857045542259385 2213 18 -2.9037580426675333 2213 19 -2.25490975430996 2213 20 -3.1626649367819937 2213 21 -1.674210541866337 2213 22 -1.4320876787707526 2213 23 -3.349294570943414 2213 24 -1.8327617128267235 2213 25 -1.1887075780451042 2214 1 -0.5187774976256776 2214 2 -0.1264899750021442 2214 3 -0.9041020105375424 2214 4 -1.5038914303926916 2214 5 -2.1102082167480454 2214 6 -0.6738164821823531 2214 7 -1.3376016503706118 2214 8 -1.971868615107521 2214 9 -8.28748272005226 2214 10 -3.4825140208609078 2214 11 -2.9773621968529973 2214 12 -2.03491695796437 2214 13 -1.9769250658512085 2214 14 -3.020736470860136 2214 15 -5.395480715638659 2214 16 -4.535534750542439 2214 17 -4.891862189693921 2214 18 -4.4887334121256615 2214 19 -6.890377734605526 2214 20 -4.401581192939627 2214 21 -6.041205615757963 2214 22 -7.975191127046614 2214 23 -9.120230531534549 2214 24 -11.441136097063175 2214 25 -5.568017922872691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 895.0 3 0.0 4 14.0 5 105.0 6 153.0 7 95.0 8 85.0 9 58.0 10 66.0 11 44.0 12 59.0 13 47.0 14 54.0 15 54.0 16 43.0 17 55.0 18 58.0 19 76.0 20 100.0 21 104.0 22 121.0 23 185.0 24 194.0 25 219.0 26 250.0 27 291.0 28 344.0 29 384.0 30 491.0 31 610.0 32 758.0 33 952.0 34 1276.0 35 1723.0 36 2175.0 37 3143.0 38 4308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.79948914431673 17.89527458492976 9.369093231162196 25.936143039591315 2 14.58865610628513 21.972406745017885 32.8921819110884 30.546755237608586 3 22.375408496732028 27.527573529411764 26.61356209150327 23.483455882352942 4 13.756127450980394 19.14828431372549 29.13092320261438 37.96466503267974 5 12.71511004442629 37.89000663841087 32.89077260889547 16.504110708267376 6 34.1465914915428 30.999487442337266 16.417221937467964 18.436699128651973 7 25.148383135489155 34.54257060990585 19.658207122390504 20.65083913221449 8 29.992321474276938 30.96493473253135 18.520604044023546 20.522139749168158 9 29.154295075253405 14.02170574383127 18.4805979318112 38.343401249104126 10 19.301292200827415 28.78083661065427 27.565248480514835 24.352622708003473 11 31.40187870124566 25.331835817847665 16.928731876659178 26.3375536042475 12 25.501608209526726 29.325573084188495 25.843671823148007 19.329146883136776 13 32.96237684414722 20.179692684669966 22.660676910511 24.197253560671804 14 24.936152824599038 24.609255286546123 23.44468280723261 27.00990908162223 15 33.947623666343354 21.34361121037317 22.50752973607637 22.201235387207106 16 20.819931587277278 25.940674937458517 29.98417317608618 23.255220299178024 17 24.82001531784529 27.388307378095483 26.586673474597905 21.205003829461322 18 25.773511692024915 21.132441539875423 30.511589911161035 22.58245685693863 19 23.239904017971103 26.149997447286466 32.0671874202277 18.54291111451473 20 24.46520651452494 22.00949609434829 31.41879818246796 22.106499208658803 21 21.27724743478483 24.677114707233653 32.2681096533769 21.777528204604625 22 23.36515391291031 23.145642962887333 26.96411251212415 26.525090612078206 23 20.302210424217673 23.753126754811372 31.47174434631681 24.472918474654143 24 22.646518276495815 28.10904635491117 28.61956299775373 20.624872370839288 25 23.26816070243504 21.32319158711522 27.301036295880344 28.1076114145694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 48.0 2 44.5 3 41.0 4 41.0 5 41.0 6 75.0 7 109.0 8 109.0 9 109.0 10 128.0 11 147.0 12 147.0 13 147.0 14 123.5 15 100.0 16 100.0 17 100.0 18 143.0 19 186.0 20 186.0 21 186.0 22 315.0 23 444.0 24 444.0 25 444.0 26 570.0 27 696.0 28 696.0 29 696.0 30 832.5 31 969.0 32 969.0 33 969.0 34 978.5 35 988.0 36 988.0 37 988.0 38 934.0 39 880.0 40 880.0 41 880.0 42 1061.5 43 1243.0 44 1243.0 45 1243.0 46 2527.5 47 3812.0 48 3812.0 49 3812.0 50 3608.0 51 3404.0 52 3404.0 53 3404.0 54 2703.5 55 2003.0 56 2003.0 57 2003.0 58 1743.0 59 1483.0 60 1483.0 61 1483.0 62 1309.0 63 1135.0 64 1135.0 65 1135.0 66 1008.5 67 882.0 68 882.0 69 882.0 70 733.0 71 584.0 72 584.0 73 584.0 74 428.5 75 273.0 76 273.0 77 273.0 78 198.0 79 123.0 80 123.0 81 123.0 82 76.0 83 29.0 84 29.0 85 29.0 86 17.5 87 6.0 88 6.0 89 6.0 90 5.0 91 4.0 92 4.0 93 4.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07146868140282811 2 0.09699321047526674 3 0.025524529072438615 4 0.025524529072438615 5 0.03062943488692634 6 0.4032875593445301 7 0.22972076165194752 8 0.275664913982337 9 0.28076981979682475 10 0.05104905814487723 11 0.005104905814487723 12 0.010209811628975446 13 0.0 14 0.05615396395936495 15 0.0 16 0.010209811628975446 17 0.02041962325795089 18 0.01531471744346317 19 0.010209811628975446 20 0.010209811628975446 21 0.0 22 0.0 23 0.0 24 0.005104905814487723 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 19589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.147684925213134 #Duplication Level Percentage of deduplicated Percentage of total 1 93.1770412287793 58.83914441778549 2 4.721099434114794 5.96252999132166 3 0.8084074373484237 1.5314717443463168 4 0.35569927243330635 0.8984634233498392 5 0.137429264349232 0.43391699423145647 6 0.07275666936135812 0.275664913982337 7 0.04042037186742118 0.1786717035070703 8 0.0646725949878739 0.32671397212721426 9 0.056588520614389654 0.3216090663127265 >10 0.30719482619240096 3.8388891724947674 >50 0.14551333872271624 6.820154168155597 >100 0.09700889248181083 13.829189851447241 >500 0.016168148746968473 6.743580580938281 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 705 3.5989585992138444 No Hit GTACATGGGAAGCAGTGGTATCAAC 616 3.144621981724437 No Hit GTATCAACGCAGAGTACTTTTTTTT 438 2.2359487467456223 No Hit GGTATCAACGCAGAGTACTTTTTTT 319 1.6284649548215835 No Hit GAGTACATGGGAAGCAGTGGTATCA 267 1.363009852468222 No Hit CATGTACTCTGCGTTGATACCACTG 250 1.2762264536219308 No Hit TATCAACGCAGAGTACTTTTTTTTT 236 1.2047577722191025 No Hit ACGCAGAGTACATGGGAAGCAGTGG 212 1.0822400326713972 No Hit GCGTTGATACCACTGCTTCCCATGT 210 1.0720302210424217 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 191 0.9750370105671551 No Hit GTATCAACGCAGAGTACATGGGAAG 159 0.8116800245035478 No Hit ACTCTGCGTTGATACCACTGCTTCC 155 0.791260401245597 No Hit GGTATCAACGCAGAGTACATGGGAA 144 0.735106437286232 No Hit TATCAACGCAGAGTACATGGGAAGC 128 0.6534279442544285 No Hit GTGGTATCAACGCAGAGTACATGGG 100 0.5104905814487722 No Hit GCTTCCCATGTACTCTGCGTTGATA 99 0.5053856756342846 No Hit CATGGGAAGCAGTGGTATCAACGCA 90 0.45944152330389504 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 89 0.45433661748940735 No Hit CTGCTTCCCATGTACTCTGCGTTGA 83 0.42370718260248097 No Hit CAGTGGTATCAACGCAGAGTACATG 79 0.4032875593445301 No Hit GCAGAGTACATGGGAAGCAGTGGTA 79 0.4032875593445301 No Hit GCAGTGGTATCAACGCAGAGTACAT 75 0.3828679360865792 No Hit GGGAAGCAGTGGTATCAACGCAGAG 74 0.37776303027209146 No Hit ACATGGGAAGCAGTGGTATCAACGC 73 0.37265812445760377 No Hit GATACCACTGCTTCCCATGTACTCT 73 0.37265812445760377 No Hit ATACCACTGCTTCCCATGTACTCTG 64 0.32671397212721426 No Hit CCACTGCTTCCCATGTACTCTGCGT 63 0.3216090663127265 No Hit GTACTCTGCGTTGATACCACTGCTT 63 0.3216090663127265 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 61 0.31139925468375107 No Hit CCATGTACTCTGCGTTGATACCACT 59 0.30118944305477563 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 57 0.2909796314258002 No Hit GTTGATACCACTGCTTCCCATGTAC 55 0.28076981979682475 No Hit CTTCCCATGTACTCTGCGTTGATAC 46 0.23482566746643527 No Hit AAGCAGTGGTATCAACGCAGAGTAC 43 0.21951095002297208 No Hit GTGGTATCAACGCAGAGTACTTTTT 42 0.21440604420848436 No Hit TACCACTGCTTCCCATGTACTCTGC 41 0.20930113839399664 No Hit ATCAACGCAGAGTACTTTTTTTTTT 38 0.19398642095053348 No Hit ATCAACGCAGAGTACATGGGAAGCA 37 0.18888151513604573 No Hit ATGGGAAGCAGTGGTATCAACGCAG 34 0.17356679769258257 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 30 0.15314717443463166 No Hit GTACATGGTAAGCAGTGGTATCAAC 25 0.12762264536219306 No Hit TCAACGCAGAGTACATGGGAAGCAG 22 0.1123079279187299 No Hit TTCCCATGTACTCTGCGTTGATACC 21 0.10720302210424218 No Hit CTCTGCGTTGATACCACTGCTTCCC 20 0.10209811628975446 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCAGTG 115 1.0007546E-4 9.060046 11 AAGCAGT 115 1.0007546E-4 9.060046 10 GCAGTGG 120 1.5911151E-4 8.682546 12 CTCTGCG 155 3.4116529E-6 8.577171 9 CTGCGTT 155 3.5339217E-6 8.555235 11 TGCGTTG 145 1.4566542E-5 8.492019 12 GAAGCAG 105 0.0041349176 8.13956 9 ACTCTGC 155 3.2626343E-5 7.9645166 8 TCTGCGT 155 3.37067E-5 7.944147 10 GGAAGCA 110 0.006286003 7.7695804 8 GTGGTAT 125 0.0023316862 7.577493 15 TACTCTG 165 6.896143E-5 7.5010514 7 AGTACTT 165 7.357442E-5 7.462683 12 GAGTACT 165 7.357442E-5 7.462683 11 CCATGTA 155 2.737829E-4 7.389757 2 GTACTCT 170 9.673842E-5 7.2991967 6 GTATCAA 275 7.592462E-9 7.2889876 1 TGGTATC 130 0.0034255867 7.286052 16 AGTGGTA 130 0.0034255867 7.286052 14 TGTACTC 160 3.947549E-4 7.1588273 5 >>END_MODULE