FastQCFastQC Report
Thu 26 May 2016
SRR1926619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635421
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG402.0492227E-523.3132531
ACACCGT400.002854331615.6271496
GTACTAC953.0421506E-715.4253091
GTCCTAC1507.2759576E-1215.0981051
GTCCTAA902.9407493E-614.8020641
ATGCTAC550.004624144614.5329361
TAGACCC450.00515662414.2973514
TATAACA450.00515662414.2973514
GTCCTGA751.5408263E-414.2099821
GTAGGAC1850.014.2001223
GTGTAGG600.00822981213.3218581
GTGTAAG600.00822981213.3218581
GGTTACA600.00822981213.3218581
AGACAAT550.001531651813.2593996
CCTACAG2500.013.1351143
TCCTGAA956.394399E-512.9263072
TGTAGGA1801.05501385E-1012.886412
TAGGACA1253.0122465E-912.8676164
GTATAGC958.001167E-512.62070751
CAATAAG750.002481900612.4337341
Produced by FastQC (version 0.11.5)