##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926619_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635421 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.569551525681398 27.0 2.0 30.0 2.0 31.0 2 16.02420599885745 16.0 2.0 30.0 2.0 31.0 3 19.410088744312826 22.0 12.0 30.0 2.0 31.0 4 21.511615133903348 27.0 2.0 35.0 2.0 37.0 5 22.284387831060037 29.0 2.0 35.0 2.0 37.0 6 22.49074235821605 30.0 2.0 35.0 2.0 36.0 7 22.22583137793683 29.0 2.0 35.0 2.0 36.0 8 22.648993344569977 30.0 2.0 35.0 2.0 37.0 9 25.369503053880813 31.0 17.0 37.0 2.0 39.0 10 26.04826406429753 31.0 17.0 37.0 2.0 39.0 11 23.244354530303532 30.0 7.0 37.0 2.0 39.0 12 24.36081904752912 30.0 8.0 37.0 2.0 39.0 13 21.12554668479638 27.0 2.0 35.0 2.0 39.0 14 24.337683205307975 30.0 10.0 37.0 2.0 40.0 15 25.37073058649305 31.0 10.0 38.0 2.0 40.0 16 25.7328700184602 31.0 7.0 38.0 2.0 40.0 17 25.031344573125534 31.0 7.0 38.0 2.0 40.0 18 25.072218261593495 31.0 8.0 38.0 2.0 40.0 19 25.458269399343113 31.0 2.0 38.0 2.0 40.0 20 25.68936500367473 31.0 2.0 38.0 2.0 40.0 21 23.59272985941604 30.0 2.0 37.0 2.0 40.0 22 24.48374856984582 30.0 2.0 38.0 2.0 40.0 23 24.436932364526825 31.0 2.0 38.0 2.0 40.0 24 23.581733999977967 30.0 2.0 38.0 2.0 40.0 25 23.37680215164434 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.5752410075716945 1101 2 10.093510922721972 1101 3 6.7609403088188955 1101 4 8.384572662349743 1101 5 7.9853493046521145 1101 6 7.246016649687533 1101 7 6.876463491111775 1101 8 5.363964834332947 1101 9 5.555744977482053 1101 10 4.890712427084445 1101 11 7.780950769407774 1101 12 5.435670874481904 1101 13 9.681904952041904 1101 14 7.436408962681089 1101 15 6.606618644997933 1101 16 5.998517363179765 1101 17 2.243671725566898 1101 18 4.719364915167862 1101 19 5.164287452188237 1101 20 3.321767821327356 1101 21 0.6380585744960534 1101 22 4.046798207808589 1101 23 5.111534985499919 1101 24 6.6055605068529 1101 25 7.153812133595164 1102 1 5.982464093021186 1102 2 10.571979032104128 1102 3 7.19739051812023 1102 4 8.41528603836904 1102 5 8.41778613267973 1102 6 6.924573333693807 1102 7 6.859221464704525 1102 8 7.354096520278354 1102 9 6.432338507531121 1102 10 5.795424647129835 1102 11 8.423975017336652 1102 12 5.831524381061197 1102 13 10.245749991487635 1102 14 7.5888098604374505 1102 15 5.680273198959643 1102 16 6.20713061984851 1102 17 7.163864487969224 1102 18 6.871618188550688 1102 19 5.730797236681671 1102 20 6.178054242637312 1102 21 4.980857382491791 1102 22 6.2198640157517815 1102 23 6.704004140763228 1102 24 8.021388341803846 1102 25 7.849763472598276 1103 1 4.999332805373875 1103 2 8.72695491149343 1103 3 6.064200065108064 1103 4 8.011225247124663 1103 5 7.454550207708039 1103 6 6.7756932282919635 1103 7 6.765799650905958 1103 8 6.486773045857074 1103 9 5.938431308384665 1103 10 4.945031896139991 1103 11 5.555007824595176 1103 12 4.491305269586515 1103 13 8.422310823081688 1103 14 6.757353424069738 1103 15 5.870895255026156 1103 16 5.588372183581033 1103 17 3.1226262711360206 1103 18 2.156311900542356 1103 19 3.9443200690759177 1103 20 4.531598090840987 1103 21 6.567302949931385 1103 22 2.609037900762857 1103 23 4.516713693612381 1103 24 5.715700057055766 1103 25 1.8474461270801896 1104 1 5.3475747492077375 1104 2 9.901276173661486 1104 3 6.766080756618642 1104 4 7.402991280953348 1104 5 7.5131138889749565 1104 6 6.628465466026306 1104 7 6.593623870749969 1104 8 6.5995754968665885 1104 9 5.769713929810628 1104 10 4.835479069586256 1104 11 7.4477540924918735 1104 12 4.491475855774972 1104 13 8.738655695736124 1104 14 6.489360824624779 1104 15 6.109165120678924 1104 16 5.675579916821576 1104 17 6.131132665996368 1104 18 3.5766922822819964 1104 19 4.512698821335551 1104 20 3.7079646898955474 1104 21 2.675244118913554 1104 22 4.756366656841578 1104 23 5.336396454223095 1104 24 6.137838919305299 1104 25 5.18491313435344 1105 1 3.2456752328846648 1105 2 9.037531483059222 1105 3 6.170553335983396 1105 4 5.331574050269737 1105 5 2.621204633641007 1105 6 4.574669297795122 1105 7 5.412259754503825 1105 8 2.8137813582780105 1105 9 3.7835024050416237 1105 10 3.5832018596901136 1105 11 6.5162267045928 1105 12 3.7120840938324093 1105 13 4.366669674081148 1105 14 3.6041690315828525 1105 15 3.9610494965229037 1105 16 4.121949066254015 1105 17 4.837650437645205 1105 18 2.6146907927862166 1105 19 2.689519157044984 1105 20 1.7322704784689869 1105 21 2.5216329100485986 1105 22 3.9051558277162073 1105 23 1.5754081492201912 1105 24 4.430662332214808 1105 25 4.872975071189 1106 1 6.059462315087103 1106 2 10.57855836508578 1106 3 7.309112183516945 1106 4 8.624816058102155 1106 5 8.230182338451176 1106 6 7.20068551938046 1106 7 6.909262493952518 1106 8 6.9350462496806955 1106 9 6.110378579429991 1106 10 5.616092642362627 1106 11 7.594939986173962 1106 12 4.931291393545813 1106 13 4.662790317377091 1106 14 5.456204796255985 1106 15 5.64055297935548 1106 16 5.531403327583501 1106 17 6.014547331882241 1106 18 5.907699504612662 1106 19 5.769481956217454 1106 20 5.268916931420694 1106 21 5.622151855265955 1106 22 6.063593867460359 1106 23 6.407015882943249 1106 24 7.3080142722885775 1106 25 6.162008260906891 1107 1 3.927707507842264 1107 2 -13.494136670375212 1107 3 -5.268832195776069 1107 4 1.4671938060964216 1107 5 4.381908385993107 1107 6 4.494206674980692 1107 7 4.5555693385756655 1107 8 5.184273739861446 1107 9 3.343027357375302 1107 10 2.9315605227073718 1107 11 5.5968852217429195 1107 12 3.0333999730584793 1107 13 4.031777771073038 1107 14 3.744916380180335 1107 15 3.5215492378018958 1107 16 3.3969168139296215 1107 17 3.9787419671273057 1107 18 -0.9571996968169465 1107 19 1.6815647543783214 1107 20 2.0962772746756606 1107 21 -1.5741703464801056 1107 22 1.623665813254366 1107 23 -1.4310688511458416 1107 24 1.9700156311612709 1107 25 2.6685815748809425 1108 1 5.356090850978404 1108 2 10.230398316338693 1108 3 1.0352066372794546 1108 4 6.024629330375994 1108 5 7.309982921406029 1108 6 7.467263297995817 1108 7 7.647480901003256 1108 8 7.267190744589264 1108 9 6.191035063232263 1108 10 5.395400571930622 1108 11 7.321909186307138 1108 12 3.009455556898807 1108 13 3.4686806427136396 1108 14 5.238076502215566 1108 15 5.364673364557973 1108 16 5.308449794361984 1108 17 0.6172679972548494 1108 18 3.99494014309899 1108 19 5.2984757937313525 1108 20 5.494347865718922 1108 21 5.213152960869984 1108 22 1.6835621127536875 1108 23 5.066541666759967 1108 24 5.751454930876797 1108 25 6.19300993187662 1109 1 4.096077363977113 1109 2 -13.494136670375212 1109 3 -4.891787980149177 1109 4 2.658970869466458 1109 5 2.429576983631417 1109 6 3.204472921285703 1109 7 4.703997900390053 1109 8 4.7388042756154825 1109 9 4.351035971683128 1109 10 3.7732855585571805 1109 11 1.903324727455594 1109 12 2.559302430517569 1109 13 3.579042573031966 1109 14 4.464508753618862 1109 15 4.3470240859902525 1109 16 4.372091144948897 1109 17 4.811622324538249 1109 18 5.03748883693882 1109 19 3.5922819058586484 1109 20 2.6581537856739175 1109 21 1.4126959402044719 1109 22 3.9387092730585778 1109 23 4.279870387958145 1109 24 5.331216043239937 1109 25 5.517924371528405 1110 1 3.4157055989899625 1110 2 9.021014844776303 1110 3 6.037427249066052 1110 4 7.690768132032954 1110 5 8.070495039934091 1110 6 3.853866262986738 1110 7 6.223500619892771 1110 8 7.111029486302229 1110 9 5.610942731589887 1110 10 5.591108760995766 1110 11 2.6896315829932185 1110 12 5.525096465542372 1110 13 9.77155348092749 1110 14 7.5742093094732645 1110 15 7.056306256810885 1110 16 6.4360562396832215 1110 17 6.920521971899436 1110 18 7.122582375878512 1110 19 6.0196861255705585 1110 20 6.066300339974319 1110 21 4.090682910499137 1110 22 5.971379167266935 1110 23 6.340918741314191 1110 24 7.164639030509079 1110 25 7.539662462497265 1111 1 4.47279888062312 1111 2 8.930929061438984 1111 3 6.841880192169302 1111 4 7.887795372004977 1111 5 7.878064157932656 1111 6 7.1047714586772095 1111 7 7.0870289797401504 1111 8 7.162815532566803 1111 9 5.4791736826429265 1111 10 4.810573345491026 1111 11 6.443079294109641 1111 12 6.009481950541353 1111 13 8.023046500518667 1111 14 5.780070728218117 1111 15 5.1707150245938855 1111 16 4.915900253883063 1111 17 6.317905093208935 1111 18 6.097384737797437 1111 19 5.12008892658686 1111 20 4.3851744447379915 1111 21 7.320769933224195 1111 22 6.812300209414971 1111 23 6.623369896157982 1111 24 6.601948142664817 1111 25 6.821089351899612 1112 1 -1.8416765406706581 1112 2 7.363006186767645 1112 3 5.596875255349502 1112 4 0.01671293266129581 1112 5 4.056505232273398 1112 6 2.9409577931162403 1112 7 5.582075518974001 1112 8 5.54191337035067 1112 9 4.434659099610055 1112 10 3.3796999166755306 1112 11 -0.37835205081319856 1112 12 3.830530553254942 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2204 6 4.027442020562493 2204 7 5.070732943125094 2204 8 5.3021810014537465 2204 9 3.7982154588626145 2204 10 3.6227451246321287 2204 11 5.0489447143063515 2204 12 5.271076263522172 2204 13 3.450745400119409 2204 14 4.806249713513665 2204 15 5.083578984083612 2204 16 5.223086542713524 2204 17 -0.5013770179995554 2204 18 3.3543803556764935 2204 19 4.229302287186723 2204 20 4.730663976337954 2204 21 6.3495111933274195 2204 22 5.5944094702972365 2204 23 4.351181367576817 2204 24 6.108962262832314 2204 25 6.2882281190629215 2205 1 6.103811463840014 2205 2 10.087258678461996 2205 3 7.174832307401342 2205 4 8.111330343292838 2205 5 8.15966581747696 2205 6 7.781123329778687 2205 7 7.942684708844304 2205 8 7.5248028791453905 2205 9 6.514356716399224 2205 10 5.918138750816453 2205 11 7.760048934805919 2205 12 7.259596504693576 2205 13 10.218393978930777 2205 14 7.827603718681626 2205 15 6.824491990112897 2205 16 6.2440822360813115 2205 17 7.11494684074291 2205 18 7.050097840603279 2205 19 5.478976705791371 2205 20 5.90533408831039 2205 21 -0.6216653785933381 2205 22 4.088707489246421 2205 23 5.462099541564758 2205 24 7.387487671962372 2205 25 7.685719936461716 2206 1 4.152729073666677 2206 2 8.337737235053858 2206 3 5.850836295750057 2206 4 2.00174126086948 2206 5 5.6656709673341865 2206 6 6.138100903613672 2206 7 6.081136523588221 2206 8 5.876268567128001 2206 9 4.858063678360573 2206 10 3.9258484521277524 2206 11 3.654025080136279 2206 12 4.693517983701195 2206 13 7.935909828996621 2206 14 0.10880040430954097 2206 15 2.2950598169304683 2206 16 3.143484285465032 2206 17 4.3110471890446185 2206 18 -0.8393009052692157 2206 19 1.0166593605472976 2206 20 2.679741228721685 2206 21 3.4105612030569397 2206 22 -2.092082065020918 2206 23 -2.8911762895549202 2206 24 1.0024302527136122 2206 25 2.5525620361674157 2207 1 1.2179394762238402 2207 2 -13.494136670375212 2207 3 -6.129550217911415 2207 4 -4.300684859420038 2207 5 0.9815090259480748 2207 6 1.5332563173767895 2207 7 2.6847671311592833 2207 8 2.806425756698502 2207 9 0.307912428559586 2207 10 0.23184302673003288 2207 11 -1.7800848117231745 2207 12 -2.2942702788243707 2207 13 -0.08493447556046618 2207 14 -5.528296518032565 2207 15 -1.817916742412116 2207 16 -0.9278909242639415 2207 17 0.27026944664691044 2207 18 -0.021570693274558295 2207 19 -0.39070625982182605 2207 20 -0.447705099031122 2207 21 -4.2713411765249525 2207 22 -4.739634818747053 2207 23 -1.8808567054612553 2207 24 -0.297482181612132 2207 25 0.3455870934218943 2208 1 5.13661278404809 2208 2 6.3317638442045485 2208 3 6.492563517409867 2208 4 8.39650474342994 2208 5 4.041163993621911 2208 6 6.448301178176369 2208 7 6.990777158867424 2208 8 6.8601432656046875 2208 9 5.260742617238829 2208 10 4.809805158937447 2208 11 6.940937090902501 2208 12 6.513108662988532 2208 13 8.888610847041459 2208 14 3.2236661419489643 2208 15 4.969208867826705 2208 16 5.466950083784614 2208 17 6.129403237384473 2208 18 1.3423460503591684 2208 19 3.7325125025478805 2208 20 4.716976917065988 2208 21 7.232897509317507 2208 22 6.284758241023365 2208 23 2.1874405633077103 2208 24 3.472243375849274 2208 25 4.981076603911404 2209 1 2.7299682252525876 2209 2 4.305863329624788 2209 3 4.683083814722618 2209 4 0.4691115663763874 2209 5 4.547262716701766 2209 6 5.136775353895828 2209 7 5.231621832013985 2209 8 4.127069890342636 2209 9 3.8844376810848367 2209 10 2.9134117912987954 2209 11 -0.7323886190269846 2209 12 2.503965152411059 2209 13 1.7316342890082979 2209 14 3.3289163801803348 2209 15 3.6871345396391675 2209 16 3.141308764935747 2209 17 0.5644007597782235 2209 18 2.9454914667876047 2209 19 3.5493022871867232 2209 20 3.636441754115733 2209 21 5.651733415549639 2209 22 4.5588539147416824 2209 23 -0.0274852990898502 2209 24 -3.735482181612131 2209 25 -0.6006607698259678 2210 1 -2.6628902453853485 2210 2 -13.494136670375212 2210 3 -7.677397807584098 2210 4 -2.6542504953050674 2210 5 -1.2357960074215946 2210 6 -2.0554358839579976 2210 7 -1.7818897860679748 2210 8 -20.073818998546255 2210 9 -12.84080862225272 2210 10 -7.50247920996863 2210 11 -10.027555496931503 2210 12 -5.020037382443185 2210 13 -5.6571844689131225 2210 14 -4.58535400427256 2210 15 -6.030954180259439 2210 16 -5.639409443767253 2210 17 -4.991007774231072 2210 18 -5.393247020749364 2210 19 -10.131456479184212 2210 20 -7.352638093793015 2210 21 -5.7041398417380655 2210 22 -9.928568983441673 2210 23 -8.141416013073796 2210 24 -10.905847200623539 2210 25 -8.735394187654443 2211 1 -0.26382207208700237 2211 2 4.295534691127134 2211 3 0.5101260682437463 2211 4 1.1294934130117547 2211 5 0.6874818090335353 2211 6 -0.3644578229429847 2211 7 -19.642155945763793 2211 8 -12.759265007935927 2211 9 -8.886667170245365 2211 10 -6.932109335461767 2211 11 -9.02678611511462 2211 12 -4.76127428420866 2211 13 -6.2194267438555535 2211 14 -4.5204983303048 2211 15 -4.99842414580684 2211 16 -11.045107510494613 2211 17 -7.595511603289069 2211 18 -5.531891944792992 2211 19 -8.64342384739857 2211 20 -6.665567635555629 2211 21 -4.497224330929232 2211 22 -5.893665647073654 2211 23 -7.67194226309611 2211 24 -21.047482181612132 2211 25 -20.837105214270412 2212 1 -1.7848782744262799 2212 2 -13.494136670375212 2212 3 -7.884970135181042 2212 4 -18.985999544734725 2212 5 -19.674959505520455 2212 6 -19.83300242388195 2212 7 -9.672984462141443 2212 8 -9.665341156542402 2212 9 -5.717661227071872 2212 10 -6.0568155690094905 2212 11 -6.064347514338479 2212 12 -3.566069529669864 2212 13 -6.30591484393968 2212 14 -4.084242258226858 2212 15 -4.258167965177787 2212 16 -4.438434330761108 2212 17 -4.616904314081765 2212 18 -3.582205386134685 2212 19 -4.171812677488937 2212 20 -4.245569806577912 2212 21 -2.1636114784773355 2212 22 -3.3833939979108543 2212 23 -3.4951179259363485 2212 24 -2.5975785207258113 2212 25 -2.8631167749640554 2213 1 -18.0469206636363 2213 2 -13.494136670375212 2213 3 -8.444039099816028 2213 4 -5.847082282221956 2213 5 -19.674959505520455 2213 6 -10.315127040838028 2213 7 -19.642155945763793 2213 8 -10.238272522550341 2213 9 -8.278910860510894 2213 10 -7.194897367706993 2213 11 -6.317711510866729 2213 12 -5.28817104098356 2213 13 -18.48703237765837 2213 14 -11.952620789266948 2213 15 -7.0583799309090125 2213 16 -6.140234760203285 2213 17 -4.696661094721833 2213 18 -4.600683995702468 2213 19 -5.6481608838494815 2213 20 -5.578087357222369 2213 21 -3.782135157824552 2213 22 -4.631685184112023 2213 23 -4.42657643516975 2213 24 -3.4621910682311317 2213 25 -4.397881516619748 2214 1 -18.0469206636363 2214 2 -13.494136670375212 2214 3 -12.577445780292958 2214 4 -18.985999544734725 2214 5 -19.674959505520455 2214 6 -19.83300242388195 2214 7 -16.14098772146473 2214 8 -15.135734886396722 2214 9 -14.694105413411528 2214 10 -14.597824968825815 2214 11 -14.767814371882643 2214 12 -13.280480330454985 2214 13 -18.48703237765837 2214 14 -16.888910203412607 2214 15 -22.8275321270275 2214 16 -18.240378670162904 2214 17 -22.513821462444 2214 18 -17.716778522828182 2214 19 -15.417036238254607 2214 20 -14.373763853360904 2214 21 -12.083861600026683 2214 22 -12.989920747771299 2214 23 -12.890243346857243 2214 24 -11.89210834983643 2214 25 -11.645516429223683 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129064.0 3 1.0 4 586.0 5 2283.0 6 3025.0 7 2817.0 8 2501.0 9 2442.0 10 2320.0 11 2092.0 12 2060.0 13 2146.0 14 3193.0 15 6266.0 16 9032.0 17 9921.0 18 10207.0 19 12047.0 20 14103.0 21 16260.0 22 17091.0 23 18804.0 24 19461.0 25 19635.0 26 16625.0 27 13719.0 28 14818.0 29 17060.0 30 16531.0 31 18832.0 32 23982.0 33 29452.0 34 35856.0 35 39607.0 36 38440.0 37 37945.0 38 25197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13657350713437 17.748387623479466 11.710162280821109 26.404876588565052 2 14.418671248568154 20.35588734842201 39.94178812655528 25.283653276454555 3 19.410687725523417 23.506459724269487 28.151324960481293 28.931527589725803 4 11.50065578548528 16.877003788982805 37.47395074322355 34.14838968230837 5 12.708699592701384 37.249988170504885 36.55313991198856 13.488172324805179 6 32.19622340778784 36.800559331163896 17.47116640471078 13.532050856337488 7 28.24859149186475 30.879426151141985 21.232164825383165 19.6398175316101 8 25.11180900671365 36.98723691247049 20.2305326298364 17.670421450979457 9 25.861987478613763 15.784855235256492 19.464863553439603 38.888293732690144 10 13.897692419455012 29.671242018945378 34.37086378201802 22.060201779581593 11 33.803653051797426 22.642384139091458 23.571906220660605 19.982056588450515 12 22.875524811468203 24.88402855041109 31.293692587668744 20.94675405045196 13 27.823219417817914 20.983976588233748 25.119467873499975 26.073336120448364 14 20.377393484350417 21.787422775354624 27.744113019787413 30.09107072050754 15 22.92019410711537 30.286380906859367 23.289613325016 23.503811661009262 16 22.290635000705574 28.749965532286918 26.38539931453856 22.574000152468944 17 21.607984034522755 28.134456013943876 28.22938062501215 22.028179326521226 18 21.491401841686532 27.81205467546825 29.11471879453245 21.581824688312768 19 22.460528332737216 27.408857493229778 27.24407286290941 22.8865413111236 20 22.783755245958304 27.58959757442987 28.002171775442207 21.62447540416962 21 22.177896297076188 26.290611001347326 26.850927252657225 24.68056544891926 22 22.249869271743666 27.507623816049755 27.992317815982588 22.250189096223995 23 22.10329469851768 27.402098788966313 27.899777391622173 22.594829120893838 24 21.97981553245144 27.423840933518644 27.987559084827723 22.608784449202194 25 22.566410022513477 27.289828289464356 27.721349674113917 22.422412013908254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 684.0 1 684.0 2 1398.5 3 2113.0 4 2113.0 5 2113.0 6 3025.0 7 3937.0 8 3937.0 9 3937.0 10 4598.0 11 5259.0 12 5259.0 13 5259.0 14 6412.0 15 7565.0 16 7565.0 17 7565.0 18 10376.0 19 13187.0 20 13187.0 21 13187.0 22 18273.0 23 23359.0 24 23359.0 25 23359.0 26 29938.0 27 36517.0 28 36517.0 29 36517.0 30 43939.5 31 51362.0 32 51362.0 33 51362.0 34 57589.0 35 63816.0 36 63816.0 37 63816.0 38 68706.0 39 73596.0 40 73596.0 41 73596.0 42 75681.5 43 77767.0 44 77767.0 45 77767.0 46 77641.5 47 77516.0 48 77516.0 49 77516.0 50 73015.0 51 68514.0 52 68514.0 53 68514.0 54 60337.0 55 52160.0 56 52160.0 57 52160.0 58 44072.5 59 35985.0 60 35985.0 61 35985.0 62 28993.0 63 22001.0 64 22001.0 65 22001.0 66 16917.5 67 11834.0 68 11834.0 69 11834.0 70 8550.0 71 5266.0 72 5266.0 73 5266.0 74 3625.0 75 1984.0 76 1984.0 77 1984.0 78 1351.5 79 719.0 80 719.0 81 719.0 82 464.5 83 210.0 84 210.0 85 210.0 86 133.5 87 57.0 88 57.0 89 57.0 90 34.5 91 12.0 92 12.0 93 12.0 94 6.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.253575188733139 2 36.25139867898606 3 5.619109220501054 4 13.606884254690984 5 13.52599300306411 6 14.690260472977759 7 9.301234929283105 8 7.594807222298288 9 0.10496977594382306 10 1.4311771250871468 11 5.627450147225225 12 5.6529450553255245 13 16.054552808295604 14 1.5695106079276575 15 2.8699082970188265 16 2.974720696986722 17 2.8467740285574443 18 1.490822619963772 19 1.4398328037631742 20 0.2892570437552426 21 4.335708136810084 22 1.5860350854000733 23 0.10607140777531747 24 9.072725012235983 25 0.1085894233901618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 635421.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.7871560945966 #Duplication Level Percentage of deduplicated Percentage of total 1 97.6726981091432 93.55789979960907 2 1.810504185944049 3.468460941378863 3 0.27407167746043704 0.7875763965003245 4 0.09223375205238651 0.3533923522012906 5 0.046221454918876456 0.2213710858616889 6 0.029527386995599297 0.16970066563278188 7 0.013597791886413132 0.09117456697759856 8 0.010115214743682682 0.07751261228667983 9 0.006897939296245831 0.0594660589290496 >10 0.039969407425204585 0.7172204198612194 >50 0.001831787198309191 0.11404954591361507 >100 0.002331292935580786 0.38217555484777704 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 40 2.0492227E-5 23.313253 1 ACACCGT 40 0.0028543316 15.627149 6 GTACTAC 95 3.0421506E-7 15.425309 1 GTCCTAC 150 7.2759576E-12 15.098105 1 GTCCTAA 90 2.9407493E-6 14.802064 1 ATGCTAC 55 0.0046241446 14.532936 1 TAGACCC 45 0.005156624 14.297351 4 TATAACA 45 0.005156624 14.297351 4 GTCCTGA 75 1.5408263E-4 14.209982 1 GTAGGAC 185 0.0 14.200122 3 GTGTAGG 60 0.008229812 13.321858 1 GTGTAAG 60 0.008229812 13.321858 1 GGTTACA 60 0.008229812 13.321858 1 AGACAAT 55 0.0015316518 13.259399 6 CCTACAG 250 0.0 13.135114 3 TCCTGAA 95 6.394399E-5 12.926307 2 TGTAGGA 180 1.05501385E-10 12.88641 2 TAGGACA 125 3.0122465E-9 12.867616 4 GTATAGC 95 8.001167E-5 12.6207075 1 CAATAAG 75 0.0024819006 12.433734 1 >>END_MODULE