FastQCFastQC Report
Thu 26 May 2016
SRR1926619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635421
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14120.22221487801001227No Hit
GGTATCAACGCAGAGTACTTTTTTT8390.13203844380339963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT307.5185776E-419.0732846
GCGCCAC456.85752E-414.74831213
TTATTAC400.005133391414.3083643
ATGTCTA400.00526712914.2530189
GTAGGAC2850.012.7185463
GTCTTAA905.1619827E-712.7114951
GTGCGCC604.155479E-412.6414111
GTCCTAC3100.012.6089831
GTCCTAA1155.0258677E-912.4351581
GTTACAA701.1176035E-412.18033715
CCTAGAC550.00297256112.140433
TTAAGTA550.002977860412.1375454
TAGGGCT550.002977860412.1375454
GATATAC1103.6150595E-812.1336991
TTTCTAA2050.012.01529116
TAGGACC1353.45608E-1012.0091064
CTAAATT1950.011.65889119
TGTAGGA4050.011.5418182
CTCTTAT752.003272E-411.4403451
TCCTACA3700.011.3444672
Produced by FastQC (version 0.11.5)