##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635421 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.44476339308899 31.0 28.0 31.0 23.0 34.0 2 28.90504405740446 31.0 28.0 33.0 23.0 34.0 3 29.256113663224856 31.0 29.0 33.0 25.0 34.0 4 33.024242195331915 35.0 33.0 37.0 27.0 37.0 5 32.73284955958333 35.0 33.0 37.0 26.0 37.0 6 32.8147070997024 35.0 33.0 37.0 26.0 37.0 7 32.64395888710005 35.0 33.0 37.0 25.0 37.0 8 32.60471718750246 35.0 33.0 37.0 25.0 37.0 9 33.86042324694966 37.0 34.0 39.0 24.0 39.0 10 33.77423471997306 37.0 33.0 39.0 23.0 39.0 11 34.00389662916397 37.0 34.0 39.0 25.0 39.0 12 33.80436907184371 37.0 34.0 39.0 25.0 39.0 13 33.94427631444349 37.0 34.0 39.0 25.0 39.0 14 34.60575114766431 38.0 34.0 40.0 23.0 41.0 15 34.72892617650345 39.0 34.0 40.0 25.0 41.0 16 34.689261135530614 39.0 34.0 40.0 24.0 41.0 17 34.59463725624428 38.0 34.0 40.0 24.0 41.0 18 34.570522535452874 38.0 34.0 40.0 24.0 41.0 19 34.62783257084673 39.0 34.0 40.0 23.0 41.0 20 34.61081708032942 39.0 34.0 40.0 23.0 41.0 21 34.52704270082355 39.0 34.0 40.0 22.0 41.0 22 34.46254058332979 39.0 34.0 40.0 21.0 41.0 23 34.13244132630177 39.0 34.0 40.0 18.0 41.0 24 34.198871299500645 39.0 34.0 40.0 18.0 41.0 25 33.97835922955017 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3545363738370817 1101 2 0.4677415054601539 1101 3 0.5289868885439866 1101 4 0.2594400751384782 1101 5 0.16096710017424698 1101 6 -0.15906897328935798 1101 7 0.05327295107844776 1101 8 0.05007213163101909 1101 9 0.4459190499538721 1101 10 0.35199138202393243 1101 11 0.05650136027641395 1101 12 0.46049136117476763 1101 13 0.17172534115803728 1101 14 0.4698536389154526 1101 15 0.22784716922910775 1101 16 0.21000325398769348 1101 17 0.20712078895807906 1101 18 0.20302931141844738 1101 19 0.286112773648469 1101 20 0.24052133569475842 1101 21 0.1076211066773638 1101 22 0.2691938051296958 1101 23 0.1151487307136776 1101 24 0.2842345398851407 1101 25 0.37507863446529655 1102 1 0.2738108625938622 1102 2 0.32786365837398534 1102 3 0.1897761034575538 1102 4 -0.0273440404789298 1102 5 0.13732718354448537 1102 6 -0.5127944870346894 1102 7 -0.18355157358357843 1102 8 0.10910937050476832 1102 9 0.4618249506752292 1102 10 0.4965106394983536 1102 11 0.24119446421648405 1102 12 0.41768779917378396 1102 13 0.11458470016800248 1102 14 0.424778162826847 1102 15 0.2954866554962905 1102 16 0.29999907546729077 1102 17 0.17808632742423924 1102 18 0.2729382096652202 1102 19 0.2796598370493797 1102 20 0.3444188693668764 1102 21 0.5204414077810426 1102 22 0.19079025013461148 1102 23 0.6024968214801518 1102 24 0.5914783928312488 1102 25 0.5743915556013519 1103 1 0.17058620155236426 1103 2 0.09227592501482818 1103 3 0.10838783680311792 1103 4 -0.16471641870573706 1103 5 0.09999906253963076 1103 6 -0.11097628822717098 1103 7 -0.024898352277936908 1103 8 0.24355278709435169 1103 9 0.4272966723491223 1103 10 0.6560022845673217 1103 11 0.30832188169290475 1103 12 0.3494628705502336 1103 13 0.1877195470813362 1103 14 0.4225406671909795 1103 15 0.3534398311855682 1103 16 0.4779734198700254 1103 17 0.013752721412046753 1103 18 0.21186494230148156 1103 19 0.46707600529138205 1103 20 0.2342685052429232 1103 21 0.31940233995196365 1103 22 0.2716892238937305 1103 23 0.4489786271748102 1103 24 0.1432979932590399 1103 25 0.35295678913252715 1104 1 0.2245661216059993 1104 2 0.580004752383303 1104 3 0.26374178192698494 1104 4 -0.03003904726067219 1104 5 0.024638158178227343 1104 6 -0.19196483943272824 1104 7 -0.04542131351837497 1104 8 -0.010768305336206652 1104 9 0.18089892646312933 1104 10 0.11836723716812969 1104 11 -0.0030216754959084824 1104 12 -0.11181321598194671 1104 13 -0.23679818624408 1104 14 0.1780371918156476 1104 15 0.034059203326549436 1104 16 -0.021949349567037757 1104 17 0.060040986609919855 1104 18 0.16491300347487936 1104 19 -0.09907833701823421 1104 20 -0.04077923342921963 1104 21 0.28928581515756235 1104 22 -0.02348174420410487 1104 23 0.23678044166869228 1104 24 0.07708424282408544 1104 25 0.07581024626107791 1105 1 0.9502992072356413 1105 2 0.8164286530617595 1105 3 0.8685654567258609 1105 4 0.879880012556491 1105 5 0.8593671436024479 1105 6 0.755303736298309 1105 7 0.6148279724238961 1105 8 0.6966799919736957 1105 9 1.1284621277332931 1105 10 1.0204697177514888 1105 11 0.8038537388212603 1105 12 0.7450781572185861 1105 13 0.7620110355352949 1105 14 0.9784578516131788 1105 15 1.1870214176011586 1105 16 0.9890614615534332 1105 17 0.9252571680992503 1105 18 1.027693613840782 1105 19 0.9822579534825451 1105 20 0.9932058225087061 1105 21 1.0907294802992666 1105 22 1.0526928705320842 1105 23 1.3846669126276154 1105 24 0.9696514951954143 1105 25 1.1154120107406982 1106 1 0.8027169446914328 1106 2 0.6758405256932107 1106 3 0.6909117344287736 1106 4 0.7026517589075851 1106 5 0.6715377612753741 1106 6 -0.34039349451764167 1106 7 -0.3494049819418237 1106 8 -0.24106793445977104 1106 9 -0.4124350575306224 1106 10 -0.1186276876805934 1106 11 -0.36751838966078054 1106 12 -0.13558184113647798 1106 13 -0.4840735457339562 1106 14 -0.03529158689474343 1106 15 -0.08330854994301973 1106 16 -0.02537995663321624 1106 17 -0.14293090485992366 1106 18 -0.186415970433643 1106 19 0.0064685430650541775 1106 20 -0.13144225639753415 1106 21 -0.1084807929496705 1106 22 -0.13430060034308866 1106 23 -0.011513201379003135 1106 24 -0.25151390930032136 1106 25 0.1668636654815856 1107 1 0.8148909548550556 1107 2 1.0531696893349896 1107 3 0.7467488372377318 1107 4 0.7901291885905763 1107 5 1.0202002954798743 1107 6 0.7418276108229165 1107 7 0.8648710925914145 1107 8 1.0224951972764273 1107 9 1.2130884807504998 1107 10 0.8285135606208129 1107 11 0.6536511715908659 1107 12 0.6421587072524204 1107 13 0.6277984410123807 1107 14 1.0291757536238677 1107 15 0.9073678149794517 1107 16 0.7646672316171887 1107 17 0.9653577532067601 1107 18 0.9550347657775262 1107 19 0.8819967350197828 1107 20 0.8293179677233837 1107 21 0.922175281955866 1107 22 0.8609130963490799 1107 23 1.2218615496494607 1107 24 1.2176904055858486 1107 25 1.2266261419805318 1108 1 1.3756082876862372 1108 2 1.2697163588223859 1108 3 1.0887163880166462 1108 4 0.9748682751241589 1108 5 -0.10735085540040501 1108 6 0.7291653317831717 1108 7 1.2464566981993883 1108 8 1.330839515175569 1108 9 1.8751334930601828 1108 10 1.9198478037164222 1108 11 1.4796145780694019 1108 12 1.3855848223892693 1108 13 1.3853607430674018 1108 14 1.8314155976469024 1108 15 1.545941115548942 1108 16 1.478075841192016 1108 17 1.6052104554099458 1108 18 1.5812650565217368 1108 19 1.620683407157422 1108 20 1.7353513034589838 1108 21 1.599817990257712 1108 22 1.5953438788608807 1108 23 2.0442734735796932 1108 24 1.892997489100324 1108 25 1.675237359643873 1109 1 0.6335323376999504 1109 2 -0.27128272748568705 1109 3 0.6023064635403621 1109 4 0.7530472853669394 1109 5 -0.6236907418091064 1109 6 0.3256436870678243 1109 7 0.4888855464059887 1109 8 1.0795235716882061 1109 9 1.5394408080113635 1109 10 -0.5220069734248582 1109 11 0.4213418226855197 1109 12 1.1422518908707389 1109 13 0.9957986687945564 1109 14 1.409072423344881 1109 15 1.3060332184904055 1109 16 1.373257208165981 1109 17 1.5214853526414416 1109 18 1.4436094187979691 1109 19 1.3199416220864109 1109 20 1.4597491490328238 1109 21 1.6032517598221645 1109 22 1.552462377541005 1109 23 1.4884081129858515 1109 24 1.2854840763474158 1109 25 1.6178057948197875 1110 1 0.6758310646140622 1110 2 -0.5565256896517283 1110 3 0.10529492806728769 1110 4 0.4876238199434795 1110 5 1.037602665638147 1110 6 0.7985921061700836 1110 7 0.9632803823392848 1110 8 1.230321491716893 1110 9 1.3888221958542886 1110 10 1.2565657359170856 1110 11 1.1254001575130843 1110 12 1.1666317844814031 1110 13 0.9739051776702965 1110 14 1.3225743060775343 1110 15 1.1052203561390783 1110 16 0.8721753840072353 1110 17 1.115735546891635 1110 18 1.1234432601702693 1110 19 1.2095955578941968 1110 20 1.1390928822227124 1110 21 1.253774741114377 1110 22 1.3454275320446172 1110 23 1.549420604767569 1110 24 1.3978144809855166 1110 25 1.3177340716711399 1111 1 -0.19349143292929227 1111 2 -0.3352763868073332 1111 3 0.11823767720635558 1111 4 0.15078656040682858 1111 5 0.2615919006703322 1111 6 -0.35536729502857867 1111 7 0.027862221767747997 1111 8 0.2513822305690425 1111 9 0.41372061032326 1111 10 0.18786204696800013 1111 11 -0.20505514042687878 1111 12 0.04159880791468851 1111 13 0.0029440324760230396 1111 14 0.13882002355024525 1111 15 0.0669958676078437 1111 16 0.01425259786358879 1111 17 -0.07065154931140682 1111 18 0.1731409380888067 1111 19 -0.04538810874591803 1111 20 -0.19460695577160436 1111 21 0.08056499244450777 1111 22 0.28549959358907273 1111 23 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0.3141880657660465 2204 7 0.5233207863796849 2204 8 0.5419982593936581 2204 9 1.016014115046211 2204 10 0.7868687662201168 2204 11 0.7078243999373228 2204 12 0.8180257238753441 2204 13 0.6059051776702944 2204 14 1.102897538400768 2204 15 0.7857860127047402 2204 16 0.7077713436031914 2204 17 0.7788870620431538 2204 18 0.9832412399682511 2204 19 0.9821612144598504 2204 20 0.8455979327277632 2204 21 0.9812898926295262 2204 22 0.9525386431557337 2204 23 1.3458650492120157 2204 24 1.273410440581472 2204 25 1.0638754858125523 2205 1 0.5255530910802761 2205 2 0.71032107254387 2205 3 0.6926412595996645 2205 4 0.3882424089341612 2205 5 0.4191232983215727 2205 6 0.4249408650769837 2205 7 0.33794955468287213 2205 8 0.4217708692127786 2205 9 0.8166273794734309 2205 10 0.8568084733691279 2205 11 0.6002964068279368 2205 12 0.5914693069933463 2205 13 0.4965580631138806 2205 14 0.9999449113551151 2205 15 0.820183945521272 2205 16 0.6862933074274835 2205 17 0.8084064418881169 2205 18 0.8484608782111422 2205 19 0.8753774073969751 2205 20 0.76444720060028 2205 21 0.6986576790205703 2205 22 0.7919391599515961 2205 23 0.8665610009777325 2205 24 0.8880736619423715 2205 25 0.8615051154421849 2206 1 0.290563325931263 2206 2 0.5066648850975142 2206 3 0.6197211517754724 2206 4 0.35558893519405643 2206 5 0.6031371700518093 2206 6 0.3789119420065603 2206 7 0.6560150848813393 2206 8 0.5300083780937968 2206 9 0.6622659150072963 2206 10 0.6932318718826949 2206 11 0.398491844965541 2206 12 0.37804362619097276 2206 13 0.46100383499662456 2206 14 0.45960662577185474 2206 15 0.4814201827767377 2206 16 0.537336628678112 2206 17 0.4823951375442235 2206 18 0.5491061942395064 2206 19 0.48569808933822856 2206 20 0.3234955781840725 2206 21 0.6843130488856062 2206 22 0.5003409393222995 2206 23 0.8910824066745562 2206 24 0.6531683701397526 2206 25 0.6435314500079272 2207 1 -0.9807540169710194 2207 2 -0.19061659874263626 2207 3 0.024778222550580153 2207 4 0.18107758839724397 2207 5 0.048422401457884234 2207 6 0.026311608889585614 2207 7 0.17476056881947244 2207 8 0.3349252213206242 2207 9 0.49808793012002894 2207 10 0.5789433582947083 2207 11 0.3261965833095104 2207 12 0.2589589018085192 2207 13 0.01458116834628953 2207 14 -5.135642477139246 2207 15 -2.0669047409860184 2207 16 -0.9052527434208955 2207 17 -0.7054959993399095 2207 18 -0.30932674759973366 2207 19 -0.09215269385406089 2207 20 -0.06609359796377134 2207 21 0.085320972660611 2207 22 0.0722534838705684 2207 23 0.18580366615063326 2207 24 -0.07804021986918741 2207 25 -0.20878107984400884 2208 1 0.48983906918810405 2208 2 1.0398722520292338 2208 3 0.9281460811071192 2208 4 0.9784947938333204 2208 5 0.8148658636560668 2208 6 0.7498404385504926 2208 7 0.783234641916188 2208 8 1.1714306969770405 2208 9 1.1735563296450309 2208 10 1.1563942093304647 2208 11 1.127934939293148 2208 12 1.2607236464977944 2208 13 1.078078991278069 2208 14 1.343329023058665 2208 15 1.488791920842722 2208 16 1.1360785667783446 2208 17 1.12273001992385 2208 18 1.044331386718774 2208 19 0.8907276333658913 2208 20 1.1191386226458064 2208 21 1.2152193761439207 2208 22 1.3118464380861639 2208 23 1.4030321923414206 2208 24 1.4773265831391242 2208 25 1.3548981654390104 2209 1 -0.3176234808404814 2209 2 -0.19923276035880022 2209 3 0.23458785736021426 2209 4 0.4190985674182244 2209 5 0.32317842321390344 2209 6 0.2635213990993819 2209 7 0.5002096752685787 2209 8 0.2664427038381021 2209 9 0.3191252261573254 2209 10 0.07042432177566837 2209 11 0.09893551104843823 2209 12 0.3575812794308959 2209 13 0.14901628878140372 2209 14 0.5295642050674374 2209 15 0.4355637904825116 2209 16 0.43932689915875045 2209 17 0.6891092842653777 2209 18 0.5459079066349162 2209 19 0.6941612144598537 2209 20 0.3762645993944247 2209 21 0.46573433707397527 2209 22 0.8307608653779539 2209 23 0.7183094936564629 2209 24 0.6511882183592519 2209 25 0.7634310413681078 2210 1 -0.2527667001053757 2210 2 -0.4961385482759937 2210 3 0.23536943741936156 2210 4 0.5293951453649939 2210 5 0.8984948575189335 2210 6 0.7371487755252346 2210 7 1.0447817073767283 2210 8 0.7418723616327796 2210 9 1.4074015252701244 2210 10 1.2650690197055425 2210 11 0.9591002765744179 2210 12 0.8452314695449701 2210 13 0.8924096136652295 2210 14 1.246131167467098 2210 15 0.994506756261984 2210 16 1.2108165485039137 2210 17 1.0628473493266313 2210 18 1.1471364659927517 2210 19 1.0501071375692703 2210 20 1.1923651486128435 2210 21 1.3155982998961093 2210 22 1.3519970292985306 2210 23 1.306243587446069 2210 24 1.0929195238091864 2210 25 1.1426274925721671 2211 1 -1.329717377554097 2211 2 -0.49186499823047214 2211 3 0.35790554124127993 2211 4 0.4059905861975679 2211 5 0.6368059662499022 2211 6 0.45443219722144335 2211 7 0.6338309585236601 2211 8 0.5109873047770677 2211 9 0.7454163059695276 2211 10 0.574662193450159 2211 11 0.46062565189350835 2211 12 0.5244920775529636 2211 13 0.502543675322876 2211 14 0.9897958169610206 2211 15 0.49637130221960746 2211 16 0.7583754124607864 2211 17 0.6635944172857222 2211 18 0.563022147636481 2211 19 0.6231252207196292 2211 20 0.48036475588894945 2211 21 0.7354933355090267 2211 22 0.7197624303231791 2211 23 0.9660090241728909 2211 24 0.9976295329132441 2211 25 1.0634873793962782 2212 1 1.091262839005374 2212 2 0.2146426410541764 2212 3 0.4318261361013853 2212 4 0.4975263130829646 2212 5 0.49800437054852154 2212 6 0.5364154260743277 2212 7 0.7454839206121875 2212 8 0.7603617789826131 2212 9 1.1965818735561626 2212 10 0.6458283037923707 2212 11 0.5831744320503631 2212 12 0.776032146482919 2212 13 0.792209609269527 2212 14 0.8470619571547857 2212 15 0.7904308425056357 2212 16 0.5534797572191223 2212 17 1.1184914294163093 2212 18 0.5350973735584859 2212 19 0.617863205468197 2212 20 0.7376390374162654 2212 21 0.9404215560848925 2212 22 0.9454917581204043 2212 23 1.1623942720764973 2212 24 1.010718083484491 2212 25 0.8288568602505748 2213 1 0.009709171525877025 2213 2 -0.09881373868135057 2213 3 -0.8178692984677269 2213 4 -1.477669563327435 2213 5 -2.246315164347095 2213 6 -1.1506645048842792 2213 7 -0.7104342924989879 2213 8 -0.5452998906460635 2213 9 -1.0927916958719592 2213 10 -1.2262738963607518 2213 11 -1.1045510398538454 2213 12 -1.5034851148489778 2213 13 -1.304406024236414 2213 14 -8.625025285342279 2213 15 -4.234491367842306 2213 16 -2.6421703202261106 2213 17 -1.93975191196661 2213 18 -6.058806200730874 2213 19 -3.5194136351599425 2213 20 -5.334587744266905 2213 21 -2.6209727336330992 2213 22 -2.235959370690601 2213 23 -5.053893434501525 2213 24 -2.8519755541977787 2213 25 -2.2510435926121417 2214 1 0.14844505498506422 2214 2 0.26095519395065025 2214 3 -1.6175409069180802 2214 4 -1.9765836755724315 2214 5 -0.5640375684787173 2214 6 -1.0339458906202488 2214 7 -0.8049793174624718 2214 8 -0.7538189784048868 2214 9 -5.4695040573317755 2214 10 -2.1202184611942165 2214 11 -1.5244912802288226 2214 12 -1.581876664494331 2214 13 -1.8086493394636634 2214 14 -2.9948428561994405 2214 15 -2.495716583349264 2214 16 -1.7172421558775923 2214 17 -2.0707744125155187 2214 18 -1.1743028929497115 2214 19 -4.365043354595187 2214 20 -2.3088963559534434 2214 21 -4.096864832188746 2214 22 -6.3726929248608855 2214 23 -6.870646892636376 2214 24 -6.045575021516136 2214 25 -4.173628148663042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19984.0 3 10.0 4 364.0 5 2349.0 6 2969.0 7 2386.0 8 1851.0 9 1465.0 10 1314.0 11 1249.0 12 1157.0 13 1131.0 14 1174.0 15 1313.0 16 1207.0 17 1270.0 18 1392.0 19 1729.0 20 2220.0 21 2955.0 22 3822.0 23 4616.0 24 5773.0 25 6674.0 26 7616.0 27 8368.0 28 9874.0 29 11879.0 30 14433.0 31 18628.0 32 23505.0 33 30662.0 34 40259.0 35 53392.0 36 72224.0 37 105593.0 38 168614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09410526912645 18.802783404157466 8.749799182871165 30.353312143844917 2 14.14385028084935 20.734679227223495 38.46644291075791 26.65502758116924 3 18.74356544435034 23.64500458093136 28.602957613004175 29.008472361714116 4 12.277735535394656 16.902725499548975 36.33889201115199 34.480646953904376 5 13.402162466767303 38.17876161078432 34.68919065690949 13.729885265538893 6 33.030980860488185 37.4395768961007 16.642023518409697 12.887418725001421 7 29.188055417563614 31.56577484446337 20.4294309212342 18.816738816738816 8 25.934588651665724 37.38985472731603 18.906109780158946 17.769446840859302 9 26.030677023776665 15.815149244903592 19.09782737273767 39.056346358582076 10 14.408192239345352 30.09713016638519 33.36187479636663 22.13280279790283 11 35.28934074062416 23.069986308053068 22.568263011284053 19.072409940038714 12 22.7595714845539 25.21014222469834 30.847509375939364 21.182776914808397 13 28.930110902850238 21.753451648592037 25.235237740018036 24.081199708539692 14 20.97110773374072 21.82992227673263 26.99540905117766 30.20356093834899 15 23.30928594360824 30.160116585774272 22.837938219611843 23.692659251005647 16 22.75990034733643 28.8526881195832 26.041733880803957 22.345677652276414 17 21.993544132667918 28.625366113149227 26.915395402201163 22.465694351981693 18 21.94759206798867 28.245357255272268 27.94932326093799 21.85772741580107 19 23.156774859617755 27.406170523506155 27.162232770125648 22.274821846750434 20 23.034975732280788 27.588399337752513 27.618143818498865 21.758481111467834 21 22.92149614192795 26.930177000760125 27.169703236122196 22.97862362118973 22 22.706677934786544 27.617437887636704 27.182135938220487 22.49374823935627 23 22.512318604515748 27.28002379524756 27.323616940579555 22.88404065965714 24 22.49436197017077 27.28381519537563 27.942123046755125 22.279699787698473 25 22.738153129972098 27.073546514830326 27.423550685293684 22.764749669903892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 179.0 3 187.0 4 187.0 5 187.0 6 304.0 7 421.0 8 421.0 9 421.0 10 730.0 11 1039.0 12 1039.0 13 1039.0 14 1737.5 15 2436.0 16 2436.0 17 2436.0 18 4287.0 19 6138.0 20 6138.0 21 6138.0 22 9793.0 23 13448.0 24 13448.0 25 13448.0 26 19050.5 27 24653.0 28 24653.0 29 24653.0 30 32482.5 31 40312.0 32 40312.0 33 40312.0 34 47715.5 35 55119.0 36 55119.0 37 55119.0 38 61468.5 39 67818.0 40 67818.0 41 67818.0 42 73228.0 43 78638.0 44 78638.0 45 78638.0 46 82549.5 47 86461.0 48 86461.0 49 86461.0 50 84323.0 51 82185.0 52 82185.0 53 82185.0 54 74625.0 55 67065.0 56 67065.0 57 67065.0 58 57722.5 59 48380.0 60 48380.0 61 48380.0 62 39619.0 63 30858.0 64 30858.0 65 30858.0 66 24029.5 67 17201.0 68 17201.0 69 17201.0 70 12571.5 71 7942.0 72 7942.0 73 7942.0 74 5605.0 75 3268.0 76 3268.0 77 3268.0 78 2221.5 79 1175.0 80 1175.0 81 1175.0 82 773.5 83 372.0 84 372.0 85 372.0 86 239.5 87 107.0 88 107.0 89 107.0 90 67.0 91 27.0 92 27.0 93 27.0 94 13.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08104862760280193 2 0.032262075065193 3 0.028170299691070957 4 0.0311604432336986 5 0.02014412491875465 6 0.3753417025877332 7 0.208680544080224 8 0.21591983897290146 9 0.21969686239516795 10 0.013849085881643823 11 0.0017311357352054778 12 0.0028327675666998727 13 0.0 14 0.07491096454161887 15 0.0014163837833499364 16 0.00220326366298879 17 0.004721279277833122 18 0.0033048954944831857 19 0.0020458876870610193 20 0.0014163837833499364 21 0.0 22 0.0 23 0.0 24 6.295039037110829E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 635421.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.03978432251876 #Duplication Level Percentage of deduplicated Percentage of total 1 92.61377035221612 80.61082596752172 2 5.667540844385374 9.866030654687375 3 0.9081780631290016 2.371428682235734 4 0.31456514705476035 1.0951873022011096 5 0.14345021164562788 0.624293774132756 6 0.09038167018669714 0.4720080647855486 7 0.06104624687618257 0.37194165132615153 8 0.039891910218951346 0.2777746609336646 9 0.02741885501358346 0.21478781040774447 >10 0.11832896735534289 1.7697991783875342 >50 0.007804671637621114 0.4734668579157716 >100 0.006534051669642466 1.1170656748159402 >500 9.07507175836578E-4 0.5123241383597167 >1k 1.815014351673156E-4 0.2230655822892137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1412 0.22221487801001227 No Hit GGTATCAACGCAGAGTACTTTTTTT 839 0.13203844380339963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.5737597592777072E-4 0.0 11 0.0 0.0 0.0 1.5737597592777072E-4 0.0 12 0.0 0.0 0.0 1.5737597592777072E-4 0.0 13 0.0 0.0 0.0 1.5737597592777072E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 30 7.5185776E-4 19.073284 6 GCGCCAC 45 6.85752E-4 14.748312 13 TTATTAC 40 0.0051333914 14.308364 3 ATGTCTA 40 0.005267129 14.253018 9 GTAGGAC 285 0.0 12.718546 3 GTCTTAA 90 5.1619827E-7 12.711495 1 GTGCGCC 60 4.155479E-4 12.64141 11 GTCCTAC 310 0.0 12.608983 1 GTCCTAA 115 5.0258677E-9 12.435158 1 GTTACAA 70 1.1176035E-4 12.180337 15 CCTAGAC 55 0.002972561 12.14043 3 TTAAGTA 55 0.0029778604 12.137545 4 TAGGGCT 55 0.0029778604 12.137545 4 GATATAC 110 3.6150595E-8 12.133699 1 TTTCTAA 205 0.0 12.015291 16 TAGGACC 135 3.45608E-10 12.009106 4 CTAAATT 195 0.0 11.658891 19 TGTAGGA 405 0.0 11.541818 2 CTCTTAT 75 2.003272E-4 11.440345 1 TCCTACA 370 0.0 11.344467 2 >>END_MODULE