FastQCFastQC Report
Thu 26 May 2016
SRR1926618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598418
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAATC250.00269257521.835293
CACCGTG654.267349E-918.7822237
CTAGGAC300.007732095718.1960753
CGAGCTC352.972482E-416.46181715
TGCCGAG455.3304975E-516.09614412
GTGCCGA403.540547E-416.08774211
GTGTTAA500.002520250915.9198841
CTTAATG603.948397E-415.85646252
TCCAGAT902.3871708E-615.1013952
TAAGACA506.0942624E-414.9776824
GTGTAAG903.0694155E-614.7406321
GCACCGT507.583647E-414.5520436
CTCTAGA658.7089144E-414.2870751
GTCCTAC955.375712E-613.964811
GTAGGAC1353.6379788E-1113.7481473
TCAGACC652.4775503E-413.4371023
GTCCTAA600.00845797213.2665691
CCCTTAC600.00845797213.2665691
AAGGCGT550.001558022813.229136
GCCGAGC552.939073E-413.16957213
Produced by FastQC (version 0.11.5)