##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926618_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 598418 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.735666373671915 27.0 2.0 31.0 2.0 31.0 2 16.16671289967882 16.0 2.0 30.0 2.0 31.0 3 19.550259183380177 22.0 12.0 30.0 2.0 31.0 4 21.680487552179248 28.0 2.0 35.0 2.0 37.0 5 22.458122917425612 29.0 2.0 35.0 2.0 37.0 6 22.67309639750141 30.0 2.0 35.0 2.0 36.0 7 22.415460430668862 30.0 2.0 35.0 2.0 36.0 8 22.823018358404994 30.0 2.0 35.0 2.0 37.0 9 25.60056849894221 31.0 17.0 37.0 2.0 39.0 10 26.27484968700808 31.0 18.0 37.0 2.0 39.0 11 23.452991053076612 30.0 7.0 37.0 2.0 39.0 12 24.536400977243332 31.0 8.0 37.0 2.0 39.0 13 21.312966855943504 27.0 2.0 35.0 2.0 39.0 14 24.550640187962262 30.0 10.0 37.0 2.0 40.0 15 25.592086133772714 31.0 10.0 38.0 2.0 40.0 16 25.97494560658269 31.0 10.0 38.0 2.0 40.0 17 25.254347295703003 31.0 8.0 38.0 2.0 40.0 18 25.299030109388422 31.0 10.0 38.0 2.0 40.0 19 25.673977052829294 31.0 8.0 38.0 2.0 40.0 20 25.917692983834044 31.0 2.0 38.0 2.0 40.0 21 23.78909391094519 30.0 2.0 37.0 2.0 40.0 22 24.693546985551905 31.0 2.0 38.0 2.0 40.0 23 24.659554024110236 31.0 2.0 38.0 2.0 40.0 24 23.812948474143493 31.0 2.0 38.0 2.0 40.0 25 23.60189533068858 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.640698414897603 1101 2 10.388868588980314 1101 3 7.185934879998669 1101 4 8.522860958980523 1101 5 8.30348813196995 1101 6 7.739371981087345 1101 7 7.254759682038536 1101 8 5.874374742599784 1101 9 6.208121861105692 1101 10 5.775944089878408 1101 11 8.347017456576111 1101 12 6.189608738244733 1101 13 10.44597618604675 1101 14 8.044150737802326 1101 15 7.195713992205398 1101 16 6.727818414816774 1101 17 2.7793613872334078 1101 18 5.034057647097683 1101 19 5.679308951195818 1101 20 3.9195629254959528 1101 21 0.703994239348976 1101 22 4.732993644271705 1101 23 6.111691262969252 1101 24 7.489449693354562 1101 25 7.78166926526805 1102 1 6.028853981652553 1102 2 10.847646472794922 1102 3 7.385810137793271 1102 4 8.704121391396338 1102 5 8.721808794497605 1102 6 7.37183998238838 1102 7 7.307714356654394 1102 8 7.615209407350637 1102 9 6.899429581182755 1102 10 6.505323202528839 1102 11 8.93923911882855 1102 12 6.333996947425618 1102 13 10.417340880070917 1102 14 8.110684084003047 1102 15 6.407579156246761 1102 16 6.7786303114344335 1102 17 7.678863490694383 1102 18 7.716921141952511 1102 19 6.264912842866686 1102 20 6.939809407515565 1102 21 5.305534037116484 1102 22 6.941177304829935 1102 23 7.31628813833785 1102 24 8.52780702794604 1102 25 8.238261479781752 1103 1 4.952930391487122 1103 2 9.053901842505185 1103 3 6.047318226633411 1103 4 7.518960985583341 1103 5 7.22174298698458 1103 6 6.27677554586521 1103 7 6.2958397565264335 1103 8 6.018386447840538 1103 9 5.44550946419162 1103 10 4.777620067532041 1103 11 5.407799579481139 1103 12 4.163027200601743 1103 13 8.087758574979976 1103 14 6.586289604522197 1103 15 5.669116492870472 1103 16 5.375160793108872 1103 17 2.8155146194760263 1103 18 1.5268270006491598 1103 19 4.11730841913942 1103 20 4.489155902351499 1103 21 6.782664098354029 1103 22 2.367465578296713 1103 23 4.387541223065021 1103 24 5.877760414290982 1103 25 2.3738852801656307 1104 1 4.9455586472093 1104 2 9.293433544688822 1104 3 5.931122488669718 1104 4 6.655040964536251 1104 5 6.820906365042987 1104 6 6.228656629790493 1104 7 6.2915303642612415 1104 8 6.496744774596653 1104 9 5.353491574162678 1104 10 4.305981025174766 1104 11 7.218186319812631 1104 12 4.077228726515976 1104 13 8.405980095632437 1104 14 6.0735034956034895 1104 15 5.402402470244844 1104 16 4.722427301922341 1104 17 5.291810331130854 1104 18 2.5380135305097227 1104 19 3.685800921112584 1104 20 2.8048886042720476 1104 21 2.1457054032737872 1104 22 4.167986493845252 1104 23 4.183679081523323 1104 24 5.35559782549856 1104 25 4.261030768195095 1105 1 3.6690229593553916 1105 2 9.30184131736055 1105 3 6.578372966286285 1105 4 5.5644349508703925 1105 5 2.55242389486175 1105 6 4.807483156012072 1105 7 5.907117015093412 1105 8 3.4036912987528645 1105 9 4.690404880301987 1105 10 4.092884865039828 1105 11 7.127051915929737 1105 12 4.950058798287547 1105 13 5.513401654641747 1105 14 4.26474977363657 1105 15 4.90584991521137 1105 16 5.182601825596635 1105 17 5.693529750762185 1105 18 2.865429581868561 1105 19 2.7060845651118264 1105 20 1.9863966678165639 1105 21 3.097546163329554 1105 22 4.457167402951807 1105 23 2.3175545741998675 1105 24 4.861525434317684 1105 25 5.610146962408621 1106 1 6.509575656334633 1106 2 11.064731162997427 1106 3 7.801443427275466 1106 4 9.169608390612769 1106 5 8.817104142682815 1106 6 7.557085872242954 1106 7 7.134004550533923 1106 8 7.39455552221186 1106 9 6.526065911489638 1106 10 5.798593850303575 1106 11 8.203717182477394 1106 12 5.389067864058138 1106 13 4.202734497933427 1106 14 5.5226726970850635 1106 15 6.00027219110482 1106 16 5.934679252177894 1106 17 6.316103169502778 1106 18 6.354611912366654 1106 19 6.411026985217536 1106 20 5.702585543871031 1106 21 6.1535736756681025 1106 22 6.452842785359312 1106 23 6.934641276569568 1106 24 7.273795727292587 1106 25 6.541226104135667 1107 1 3.337995602284817 1107 2 -13.581336252732909 1107 3 -5.450716350254668 1107 4 1.309461440542396 1107 5 3.88105144922298 1107 6 4.2061659007332715 1107 7 4.309227684946208 1107 8 5.056991240075902 1107 9 3.214020231555889 1107 10 2.5736770890667664 1107 11 5.221466549453844 1107 12 2.4459692691489074 1107 13 3.967443136702755 1107 14 3.5134354947254103 1107 15 3.24004157627963 1107 16 3.198369770967531 1107 17 3.801653533640419 1107 18 -1.317443548334765 1107 19 1.5047758137405722 1107 20 1.8481792569162572 1107 21 -1.7924830050733291 1107 22 1.497177437562712 1107 23 -1.6890010881269717 1107 24 1.8729742450462155 1107 25 2.442547700547795 1108 1 5.408604732854204 1108 2 10.76173632031139 1108 3 0.9238740365162741 1108 4 6.310866196300996 1108 5 7.656149880750569 1108 6 7.687870200503692 1108 7 7.688381383921971 1108 8 7.356182776551098 1108 9 6.2084886841548155 1108 10 5.020912558892391 1108 11 7.49411437684212 1108 12 2.9824599011089035 1108 13 3.921444675244775 1108 14 5.5004496511987355 1108 15 5.647879564545889 1108 16 5.624151412919474 1108 17 1.085695491070659 1108 18 4.411622117564917 1108 19 5.099537223950694 1108 20 5.566984545590135 1108 21 5.475273047683054 1108 22 2.266041943809931 1108 23 5.371954849024359 1108 24 6.235107930111635 1108 25 6.777221755189192 1109 1 3.418729980026434 1109 2 -13.581336252732909 1109 3 -5.532296405309987 1109 4 1.9134092770179336 1109 5 2.000315920899979 1109 6 2.8752702018941143 1109 7 3.7686672942854784 1109 8 3.839097973757543 1109 9 3.2393004220921675 1109 10 2.558301486569622 1109 11 0.8516562707350914 1109 12 1.5387118812179317 1109 13 2.6688835687077095 1109 14 3.2625704013509385 1109 15 3.366862491164415 1109 16 3.236058981678294 1109 17 3.583897378269743 1109 18 3.9850577197017962 1109 19 2.2958101001068556 1109 20 1.488849815744043 1109 21 0.40698945336431436 1109 22 2.905606980273731 1109 23 3.490831904985839 1109 24 4.404657900366551 1109 25 4.746340496182491 1110 1 3.70655014757234 1110 2 9.529184469902587 1110 3 6.9173015270736755 1110 4 8.05165872355207 1110 5 8.688198125894555 1110 6 4.48084416587432 1110 7 6.749704439487822 1110 8 7.5133330598541015 1110 9 6.166330272855149 1110 10 5.941417446207208 1110 11 3.6862055305969754 1110 12 6.500620439810287 1110 13 10.883627205110031 1110 14 8.403727993267701 1110 15 7.66341941276319 1110 16 7.239568464785407 1110 17 7.567698213423704 1110 18 7.778621950297456 1110 19 6.872039455018172 1110 20 7.137711137410452 1110 21 4.864826944363848 1110 22 6.875971220723713 1110 23 7.1715764439546525 1110 24 8.309614065421997 1110 25 8.488805577721422 1111 1 5.090437796362949 1111 2 8.877036953008718 1111 3 7.08219177413854 1111 4 7.87215305667015 1111 5 8.064846199561476 1111 6 7.294273837047712 1111 7 7.267354908041586 1111 8 7.45109549318116 1111 9 5.743586474900209 1111 10 5.161033145681959 1111 11 7.152392880916548 1111 12 6.572327724639109 1111 13 8.389567643104833 1111 14 6.110463676064686 1111 15 5.586268463706652 1111 16 5.621102921156265 1111 17 6.588606439936267 1111 18 6.437758875791559 1111 19 5.59841117603165 1111 20 5.269766519804222 1111 21 7.781246919042115 1111 22 7.039240470071604 1111 23 6.988470819784091 1111 24 6.46421290916453 1111 25 6.9818278638976885 1112 1 -2.1380403044835745 1112 2 7.02539451649786 1112 3 5.10586218267737 1112 4 -0.4991434100210519 1112 5 3.2449023276439206 1112 6 2.205209611799276 1112 7 4.862634628321306 1112 8 5.193734433188524 1112 9 3.7041449953271517 1112 10 2.6606328880448586 1112 11 -0.9535465743650136 1112 12 3.166333061407606 1112 13 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3.7919248036537816 2204 4 6.290448137760151 2204 5 1.7518481660552752 2204 6 4.176203052189269 2204 7 5.182071505516802 2204 8 5.290117686468328 2204 9 4.117964086301413 2204 10 3.7727117108995465 2204 11 5.070371806880953 2204 12 5.283896148367244 2204 13 2.458125592243544 2204 14 4.295791224368642 2204 15 4.787709667325714 2204 16 4.515474111353225 2204 17 -0.4857457541981418 2204 18 3.097330706325277 2204 19 3.8805321354665843 2204 20 4.243307590985946 2204 21 5.802806112289147 2204 22 4.870893439655607 2204 23 3.728017857334759 2204 24 5.168648445179013 2204 25 5.187883416232733 2205 1 6.212426903467708 2205 2 10.44141169995135 2205 3 7.410976097425042 2205 4 7.899998292225746 2205 5 8.123089665783489 2205 6 7.349023043590414 2205 7 7.785114855100893 2205 8 7.603852022605476 2205 9 6.662609858489464 2205 10 5.974950974417087 2205 11 7.982158087215446 2205 12 7.185732690440993 2205 13 10.512484083017586 2205 14 7.904700280894655 2205 15 6.836947854714573 2205 16 6.54140889744615 2205 17 7.173810039554887 2205 18 6.959171556305815 2205 19 5.571199835578607 2205 20 6.130253870201059 2205 21 0.06920944260637896 2205 22 4.447131941657343 2205 23 5.986158147541818 2205 24 7.556322743681335 2205 25 7.977579547681554 2206 1 3.8950131279545417 2206 2 7.788764020740201 2206 3 5.401773233795804 2206 4 1.2345437437549798 2206 5 4.891384867437765 2206 6 5.304044721539128 2206 7 5.580987128146003 2206 8 5.529689134822348 2206 9 4.330276577965115 2206 10 3.5168482656481146 2206 11 2.8869791601557075 2206 12 4.185124695935336 2206 13 7.285688861041528 2206 14 0.13418212524971906 2206 15 1.7865423729763883 2206 16 2.895954299207755 2206 17 3.8555502143885576 2206 18 -1.4422557792266915 2206 19 0.5125022377789996 2206 20 1.9353381309402913 2206 21 2.434551930971125 2206 22 -2.4850820624551204 2206 23 -3.1830434884859606 2206 24 0.3974296420550125 2206 25 1.7005762555530488 2207 1 1.0159407705685943 2207 2 -13.581336252732909 2207 3 -6.197473969010046 2207 4 -4.764224902429486 2207 5 0.5022267501811584 2207 6 1.2740760937738038 2207 7 2.5045519486062595 2207 8 2.585750695691221 2207 9 0.1681812981263171 2207 10 -0.11773384578258472 2207 11 -1.5708533671979978 2207 12 -2.4410255411684894 2207 13 -0.2124133703182558 2207 14 -5.4235282047343425 2207 15 -1.8238774044078028 2207 16 -0.7934418172523898 2207 17 0.27206502261202203 2207 18 0.33663946445600956 2207 19 -0.2537477526276959 2207 20 -0.3405959796922424 2207 21 -3.9104812533320157 2207 22 -4.5777337624311585 2207 23 -1.7334049215293916 2207 24 -0.03449670183891129 2207 25 0.351677609445332 2208 1 5.065248892658083 2208 2 7.1035453501814825 2208 3 6.712845158717723 2208 4 8.647719767784746 2208 5 3.3315686440233527 2208 6 6.373913198740567 2208 7 7.137151231936269 2208 8 6.767624022651884 2208 9 5.310802204238456 2208 10 5.139550850774299 2208 11 6.949183914635967 2208 12 6.633061028340489 2208 13 9.199280823223535 2208 14 3.4218867942177447 2208 15 5.141913786651372 2208 16 5.410232351786988 2208 17 6.250253105034183 2208 18 1.051901161461025 2208 19 3.9089087313954707 2208 20 5.052465816481085 2208 21 7.512187907877838 2208 22 6.564327404994604 2208 23 1.9963520479414214 2208 24 3.3421580203279326 2208 25 4.380187648107341 2209 1 1.940319554048699 2209 2 3.867897463742187 2209 3 4.05261652986275 2209 4 0.5110135307393371 2209 5 4.0560834358077855 2209 6 4.433425792170631 2209 7 5.052812936603061 2209 8 3.4890925233742003 2209 9 3.3985636513837356 2209 10 2.4655511020147998 2209 11 -1.3361467806721166 2209 12 2.0469954545699984 2209 13 1.1347591223253488 2209 14 2.6076634414132727 2209 15 3.2056189559246597 2209 16 2.6862828511274834 2209 17 0.06978391340049228 2209 18 2.5874673947378284 2209 19 2.8396291635291284 2209 20 3.1411537129700022 2209 21 5.12425687339508 2209 22 3.721494040256207 2209 23 -0.6674724605693534 2209 24 -3.5206960717517113 2209 25 -0.8322833022098486 2210 1 -2.6620691313681135 2210 2 -13.581336252732909 2210 3 -7.507185065665471 2210 4 -2.6833522122408766 2210 5 -0.7878304455948637 2210 6 -1.3380827421393704 2210 7 -1.488301860040032 2210 8 -20.21780402834994 2210 9 -12.652786174705222 2210 10 -7.156514869007708 2210 11 -9.91039681330443 2210 12 -4.875571902366097 2210 13 -5.321799588966966 2210 14 -4.187534233630302 2210 15 -5.938491103274199 2210 16 -5.199289058976252 2210 17 -4.566287079159338 2210 18 -4.602423882068656 2210 19 -9.888478392579255 2210 20 -7.151849918814641 2210 21 -5.280063261126838 2210 22 -9.623266361773906 2210 23 -7.667666334908066 2210 24 -10.269768930112111 2210 25 -8.125192876146995 2211 1 0.17924813780486915 2211 2 4.785030113633459 2211 3 0.4892773285925145 2211 4 1.6185348451572033 2211 5 1.0620204072620005 2211 6 -0.129516460426796 2211 7 -19.750826910140233 2211 8 -13.037623848169758 2211 9 -9.195728917046763 2211 10 -7.181138691226721 2211 11 -9.888276496594935 2211 12 -5.121923464348921 2211 13 -6.5412876140662135 2211 14 -4.926051307818717 2211 15 -5.555184951430974 2211 16 -11.588906130958051 2211 17 -7.8757909718295664 2211 18 -6.034111080288923 2211 19 -8.798733336902337 2211 20 -6.739744426548828 2211 21 -4.619443723753793 2211 22 -6.457711026879892 2211 23 -8.622159906636108 2211 24 -21.290811014280447 2211 25 -21.08611471983437 2212 1 -1.4084565320915843 2212 2 -13.581336252732909 2212 3 -7.762011895299448 2212 4 -19.098181871559515 2212 5 -19.78682844158685 2212 6 -19.980367311277647 2212 7 -9.595927015514311 2212 8 -9.42539096196427 2212 9 -5.628981980436812 2212 10 -5.784164266855036 2212 11 -5.798501790381874 2212 12 -3.2294651472330713 2212 13 -6.370104840143714 2212 14 -3.8301587872686618 2212 15 -3.954528446664881 2212 16 -3.994710249090936 2212 17 -4.152667426788213 2212 18 -3.177218360382504 2212 19 -3.684168825077549 2212 20 -3.8789057163950496 2212 21 -1.7405342558032295 2212 22 -3.195552134584016 2212 23 -3.247840381631651 2212 24 -2.33612186781048 2212 25 -3.1651452783169844 2213 1 -18.186117227560498 2213 2 -13.581336252732909 2213 3 -8.921966691347157 2213 4 -6.660054781258511 2213 5 -19.78682844158685 2213 6 -10.818717367019007 2213 7 -19.750826910140233 2213 8 -10.884470695016605 2213 9 -9.25659915183004 2213 10 -7.971989742947336 2213 11 -7.3350543781554265 2213 12 -6.2327332708109004 2213 13 -18.590336663115263 2213 14 -12.597083544068346 2213 15 -7.697919659383071 2213 16 -6.927301382396781 2213 17 -5.743953448687694 2213 18 -5.675103243020214 2213 19 -6.613323847145018 2213 20 -6.378195586739118 2213 21 -4.655593585990612 2213 22 -5.499930503881981 2213 23 -5.2045148368171255 2213 24 -4.479216811381896 2213 25 -4.936727874795352 2214 1 -18.186117227560498 2214 2 -13.581336252732909 2214 3 -11.935326341912795 2214 4 -19.098181871559515 2214 5 -19.78682844158685 2214 6 -19.980367311277647 2214 7 -15.603285926533676 2214 8 -14.438484986735816 2214 9 -13.47881528210034 2214 10 -13.367314779042152 2214 11 -13.726450105260348 2214 12 -11.840807543888738 2214 13 -18.590336663115263 2214 14 -16.238474702209324 2214 15 -23.015645411891434 2214 16 -17.71047662957773 2214 17 -22.76067585671445 2214 18 -17.227417880153098 2214 19 -14.68083485834812 2214 20 -13.27687424236717 2214 21 -10.79403319342523 2214 22 -11.835000043513173 2214 23 -11.645584339896608 2214 24 -10.613635730547786 2214 25 -10.40893943610171 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 117956.0 3 1.0 4 547.0 5 2189.0 6 2761.0 7 2629.0 8 2248.0 9 2201.0 10 2094.0 11 1950.0 12 1955.0 13 2052.0 14 3031.0 15 5925.0 16 8544.0 17 9135.0 18 9703.0 19 11505.0 20 13123.0 21 15419.0 22 16177.0 23 17819.0 24 18487.0 25 18421.0 26 15602.0 27 13069.0 28 14158.0 29 15906.0 30 15743.0 31 17915.0 32 23112.0 33 28396.0 34 33997.0 35 38146.0 36 36819.0 37 36137.0 38 23546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98615373388283 16.91395342584662 11.48471917621438 26.61517366405617 2 15.158917824164696 19.647974443696267 39.067320189793136 26.125787542345897 3 19.782902511339593 22.385524947105484 27.619605290159143 30.211967251395777 4 12.02200256580676 16.021994837473144 35.855410606365055 36.10059199035504 5 13.60357775000048 36.41115620106739 35.68177747304072 14.303488575891404 6 34.18111113282395 35.23870009575362 16.517499462607237 14.062689308815196 7 29.576110026005747 30.055947296984144 20.374968274461768 19.99297440254834 8 26.32649889586428 35.856581624723965 19.383997079732694 18.43292239967906 9 26.242104065737 15.395231658681514 18.49304756296673 39.86961671261476 10 14.542642966992162 28.851126457986094 33.360688676413986 23.24554189860776 11 34.636378273462505 22.00095897124679 22.966476700714274 20.396186054576436 12 23.508172033485405 23.962793993887583 30.53815830269744 21.990875669929576 13 28.359553951750886 20.177569759548394 24.613439406751063 26.849436881949657 14 21.35979203420365 20.719662174437932 26.566747546027376 31.353798245331042 15 23.63339515327101 29.088803939042556 22.722784030499366 24.555016877187068 16 23.624699112575804 27.70850336270752 25.12199069530392 23.544806829412757 17 22.742438678742307 27.305201170873993 27.05960656707588 22.892753583307822 18 22.602965180004546 26.984393610848116 27.63762907763152 22.775012131515812 19 23.61455916866767 26.533989933932954 26.052561963290056 23.798888934109318 20 23.591982436441032 26.605104828302807 26.868223030384286 22.93468970487188 21 23.053847255253242 25.379172821786156 26.149217557718433 25.41776236524217 22 23.26073111705515 26.20235214197767 27.099392858659193 23.437523882307985 23 23.355253802903135 26.1735759919835 26.835874178400616 23.635296026712748 24 22.962595987119148 26.377673189662293 26.672538280167707 23.987192543050853 25 23.432802611173102 26.14937288682853 26.67083794097424 23.746986561024126 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 506.0 1 506.0 2 1115.0 3 1724.0 4 1724.0 5 1724.0 6 2553.0 7 3382.0 8 3382.0 9 3382.0 10 3942.5 11 4503.0 12 4503.0 13 4503.0 14 5205.0 15 5907.0 16 5907.0 17 5907.0 18 7969.0 19 10031.0 20 10031.0 21 10031.0 22 13781.0 23 17531.0 24 17531.0 25 17531.0 26 22960.0 27 28389.0 28 28389.0 29 28389.0 30 34605.5 31 40822.0 32 40822.0 33 40822.0 34 47209.0 35 53596.0 36 53596.0 37 53596.0 38 58866.5 39 64137.0 40 64137.0 41 64137.0 42 67489.0 43 70841.0 44 70841.0 45 70841.0 46 72395.0 47 73949.0 48 73949.0 49 73949.0 50 72180.0 51 70411.0 52 70411.0 53 70411.0 54 64061.5 55 57712.0 56 57712.0 57 57712.0 58 49496.5 59 41281.0 60 41281.0 61 41281.0 62 34225.0 63 27169.0 64 27169.0 65 27169.0 66 21320.5 67 15472.0 68 15472.0 69 15472.0 70 11252.5 71 7033.0 72 7033.0 73 7033.0 74 4906.0 75 2779.0 76 2779.0 77 2779.0 78 1836.5 79 894.0 80 894.0 81 894.0 82 577.5 83 261.0 84 261.0 85 261.0 86 168.5 87 76.0 88 76.0 89 76.0 90 43.5 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.984235099880017 2 35.972179981217145 3 5.538269236553713 4 13.50928615115187 5 13.236399974599694 6 14.486195268190464 7 9.139430966314515 8 7.52717999792787 9 0.1072828691650318 10 1.44531080281676 11 5.552807569291031 12 5.680477525742876 13 15.811021727287617 14 1.583341410184854 15 2.8675608019812238 16 2.9471038638543625 17 2.8361446346867907 18 1.511318175589638 19 1.4561727755515377 20 0.28876136747223513 21 4.298667486606353 22 1.603060068380296 23 0.10878683462061636 24 8.927371837077093 25 0.11346583826021277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 598418.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.17467472662936 #Duplication Level Percentage of deduplicated Percentage of total 1 96.91227448129709 91.26681926293976 2 2.246607204817637 4.23147005504406 3 0.3979761518650495 1.1243782395254005 4 0.15676117112325832 0.5905172920119335 5 0.08069217339007309 0.3799579590997455 6 0.05452610341846896 0.308098683212692 7 0.034773997888662145 0.22923809580764776 8 0.01983502094014132 0.1494365316186953 9 0.015844888753537317 0.13429685199996044 >10 0.07636941356211602 1.185129443842727 >50 0.002892932268614168 0.20705496165808876 >100 0.0014464606753077924 0.19360262323930355 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.6710727284272866E-4 0.0 7 0.0 0.0 0.0 1.6710727284272866E-4 0.0 8 0.0 0.0 0.0 1.6710727284272866E-4 0.0 9 0.0 0.0 0.0 1.6710727284272866E-4 0.0 10 0.0 0.0 0.0 1.6710727284272866E-4 0.0 11 0.0 0.0 0.0 1.6710727284272866E-4 0.0 12 0.0 0.0 0.0 1.6710727284272866E-4 0.0 13 0.0 0.0 0.0 1.6710727284272866E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAATC 25 0.002692575 21.83529 3 CACCGTG 65 4.267349E-9 18.782223 7 CTAGGAC 30 0.0077320957 18.196075 3 CGAGCTC 35 2.972482E-4 16.461817 15 TGCCGAG 45 5.3304975E-5 16.096144 12 GTGCCGA 40 3.540547E-4 16.087742 11 GTGTTAA 50 0.0025202509 15.919884 1 CTTAATG 60 3.948397E-4 15.8564625 2 TCCAGAT 90 2.3871708E-6 15.101395 2 TAAGACA 50 6.0942624E-4 14.977682 4 GTGTAAG 90 3.0694155E-6 14.740632 1 GCACCGT 50 7.583647E-4 14.552043 6 CTCTAGA 65 8.7089144E-4 14.287075 1 GTCCTAC 95 5.375712E-6 13.96481 1 GTAGGAC 135 3.6379788E-11 13.748147 3 TCAGACC 65 2.4775503E-4 13.437102 3 GTCCTAA 60 0.008457972 13.266569 1 CCCTTAC 60 0.008457972 13.266569 1 AAGGCGT 55 0.0015580228 13.22913 6 GCCGAGC 55 2.939073E-4 13.169572 13 >>END_MODULE