FastQCFastQC Report
Thu 26 May 2016
SRR1926618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598418
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11450.19133782740492433No Hit
GGTATCAACGCAGAGTACTTTTTTT7080.11831194917265189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGT508.471268E-515.2474591
AGGACGT1250.014.4887375
TGAGGCG400.00515744214.2980975
AGTTGGC400.005362157714.21446817
CGTGCCG759.83564E-713.90905110
TCCTATA551.895036E-413.870652
GACTGTC707.2186085E-613.5761577
AGCACCG801.9194795E-613.1065885
GTCCAAG801.9249237E-613.1032851
GTGCCGA802.0348762E-613.03864511
CAAGGGT603.970935E-412.7094194
GGACGTG1301.8553692E-1012.4650076
TAGGACG1301.8553692E-1012.4650074
GCACCGT1001.3749195E-712.3916856
ACAAGAC701.051697E-412.2596333
ATAAGCC550.00298072412.1357983
CCTATAC550.00298072412.1357983
CGAGCTC951.0679323E-611.97007815
CTATACT802.7624741E-511.915084
GTCCTAA802.7696145E-511.9120771
Produced by FastQC (version 0.11.5)